monsanto 08-23-05
TRANSCRIPT
Soybean Breeding at Monsanto
• Overview of Soybean Breeding• High Yielding, Diverse Germplasm Pool• Screening Capabilities and Markers• Roundup Ready™ 2nd Generation• Food Traits• Comprehensive Approach to Soy Improvements
Soy Breeding is a Key Component of Our Integrated Pipeline
ObjectivesEnhance germplasm competitivenessOutstanding germplasm key to delivering value added traits
Germplasm ResourcesSuperior Yield• Disease
resistance• Yield stability
Composition• Protein • Oil
Breeding Technology• Marker Ass’ted Breeding• Markers for Key Traits• Chromosome mapping
Soy varieties with increased value• Higher yields
• Improved consumer uses• Improvements in feed efficiency
Increased value for Monsanto and our stakeholders
Biotechnology• RR2G & Yield• Food & Feed Traits
Success: Integration of Conventional Breeding, Breeding Technology, Biotechnology, & Crop Analytics
Key DriversTop yielding varieties Increased genetic gain through germplasm & technology High value opportunities in food & feedPipeline support
Crop Analytics• HighThroughput• Essential for
Marker Development
• Ames• Beaman
• Bloomington
• Redwood Falls
•Glyndon
• Janesville • Guelph
• Stuttgart
• Stonington• Oxford
• Haubstadt
• Marion
• Mt Olive
• Galena
Global Soybean BreedingNorth America Variety Development
Northern RegionCentral RegionSouthern Region
2005 North America2005 North AmericaSoybean BreedingSoybean BreedingTesting & PurityTesting & Purity
• Findlay
Each station positioned to cover 5 million acresTypical station: 1 Breeder
+ 2-4 RA’s
Soybean Breeding at Monsanto:Excellent Germplasm from Multiple, Complementary Sources
World Class Germplasm Pool
HartzSouthern US
Food
AsgrowElite Varieties in NA & SA
YieldDefensive traits
DeKalbYield
Midwestern US
MonSoyBrazil
High Yield
Germplasm ProvidersHigh Yields
Complementary
First LineCanada
Food
Public SourcesUSDA, AVRDC
Disease, SCN, Diversity
UniversityCollaborations
Disease, Diversity
Experienced, Dedicated Breeding Staff15 Variety Development Breeders in N. America3 Breeders for South America2 Trait Breeders4 Pathology/Nematology Experts9 Leads with avg 15 years industry experienceBreeding Tech expertise
Outstanding GermplasmYield from multiple sources in
North & South AmericaPest tolerance for Yield StabilityUseful variability for protein & oil
quality and quantity Aggressive development of markers
for food, feed, and agronomic traits
Industry Collaborations
Germplasm Diversity
Elite Soy Varieties w/ Value Added
Traits
0
5
10
15
20
25
30
35
40
45
1920 1930 1940 1950 1960 1970 1980 1990 2000
Year
US
Gra
in Y
ield
(Bu/
Acr
e)
Goals• Identify what genes
control agronomic traits
• Implement new breeding procedures that improve our ability to select for these genes
• Increase the rate of genetic gain in our breeding programs
Goals• Identify what genes
control agronomic traits
• Implement new breeding procedures that improve our ability to select for these genes
• Increase the rate of genetic gain in our breeding programs
DNA markers improve the efficiency of plant breeding proceduresDNA markers improve the efficiency of plant breeding procedures
Plant Breeding Improvement for Soybeans
Mon Soybean RM012345678
Soybean Relative Maturity
Biotic and Abiotic Stresses Vary Across the Soy Belt; Diverse Germplasm, Hi Throughput Screening, and Markers are
the Foundation for Success
Scale: +++++ = severe, +++ = moderate, + = minor
+++++++++++++++++--6-8
+++++++++++++++++-+4-5
++++++++++++++++-+++3
--++++++++++++++++++++1-2
--++++++++++++++++++00-0
Root Knot Nema
StemCanker
Soy Cyst Nema
PhytopRoot Rot
Brown Stem Rot
IronDefic
White Mold
RM*
+++++++++++++++++--6-8
+++++++++++++++++-+4-5
++++++++++++++++-+++3
--++++++++++++++++++++1-2
--++++++++++++++++++00-0
Root Knot Nema
StemCanker
Soy Cyst Nema
PhytopRoot Rot
Brown Stem Rot
IronDefic
White Mold
R
RM = Relative Maturity
Broad Approach for Diseases, Nematodes, Insects & Nutritional Stresses Provides Yield Stability
• Breeding and Breeding Tech Provide Solutions for Stresses that Can Limit Yield
– Understand & prioritize key stresses by market
– Field and/or greenhouse screens for important stresses
– Automation to increase capacity – Continually search for new genes– Develop markers for useful
genes– Integrated approach across
Breeding, Breeding Tech, Biotech, Tech Development
– Prioritized application of markers
– Key partnerships with public institutions
Multiple Approaches to Identify Solutions for Asian Soybean Rust
• Monsanto Approach– Map Rpp genes– Utilize markers – Stack & evaluate major genes– Evaluate Soy Germplasm for new
sources– Validate screening protocols and
establish differential lines– Full operations of GC/GH
screening facilities in St. Louis and GH in Brazil
– Field screens in US and Brazil– Confirm USDA phenotypic
scores in FT. Detrick, MD• Identify immune PI accessions
Acquire & screengermplasm
Map genes & develop markers
Stack genes
Biotech solutions
Ag Challenge: Delivering Better Herbicide Tolerance & Increased Yields in Soybeans
DiscoveryDiscovery Phase 1Phase 1Proof of ConceptProof of Concept
Phase 2Phase 2Early DevelopmentEarly Development
Phase 3Phase 3Adv. DevelopmentAdv. Development
Phase 4Phase 4PrePre--LaunchLaunch
LaunchLaunch
Genomics
Biotechnology
MolecularBreeding
Roundup RReady2Yield® Soybeans
Yield estimates based on average yields of 64 bushels obtained in 2004.Lower yielding environments may not see the same level of yield increase
Compared with RRSB, this New Technology will deliver
better yield due to:
New Event
Breeding / Breeding Technology
Improved Tolerance
Up to+5 bu
ConventionalBreeding
Rich Pipeline of Soybean Products with Improved Food Uses
Near TermPursue commercialization of germplasm derived soy quality traits for near term profit opportunity, and development of business model
– Develop and enhance strategic relationships, and generate demand with food industry and supply chain to maximize value
– Oil composition– Improved Soy Protein
Soy Protein: Improved flavor profile
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
16.0
0.0 10.0 20.0 30.0 40.0 50.0
Off-Flavors Type A
Off-
Flav
or T
ype
BImproved
Soy
CommoditySoy
StandardSoybean
Low Lin
Linoleic18:2
Oleic18:1
Sats18:0 / 16:0
Linolenic18:3
Mid Oleic+ Low Lin
Zero Sat+ MO + LL
Progressive improvements in Oil Composition
Long TermContinue development of oil composition improvements to ensure strong position on soy trait business long term
– Multiple new traits with processing and health benefits
Low Linolenic AcidImproved Soybeans for Food
Benefits:• Cost competitive, widely available trans free
oil that doesn’t compromise taste or increase saturated fat
• A more stable soy oil requiring less hydrogenation; reduce/eliminate trans fats.
2005 technical milestones• Successful launch of AG2421V and
AG2321V• Rich Pipeline of new products to advanced
stage testing• Marker utilization for multiple traits
2006 technical milestones• More commercial varieties• Continued yield improvements• Expanded area of adaptation
Zero Sat + Mid Oleic + Low Lin Provides the Next Generation of Improvements
Product Concept: Biotech soybean with broad industry appeal that achieves reduced Sats, low linolenic, and mid oleic levels. Ideal for cooking and salad, frying segments of the oil market.
2005 Technical Milestones: Identify events that meet target composition,
Roundup® tolerance, other selection criteria
2006 Technical Milestones: Successful US field trials for most
promising transgenic events
Key Challenges for Breeding:Initiate crosses with Vistive™ as soon as
possible
Benefits: Compositional change results in healthier oil (lower sats, mid oleic – reduced cardiovascular risk) with improved functionality (low lin,mid oleic – enhanced flavor from improved stability, prolonged shelf life.)
Zero Sat + MO + LL
Zero Sat + MO + LL
Linolenic
SaturatesSaturates
CommoditySoy
CommoditySoy
Per
cent
of S
oy O
il C
ompo
sitio
nOleicOleic
LinoleicLinoleic
Lower affinity for odors, creates balanced flavors.
HBC less binding of off-flavorsGlycinin
β-conglycinin0
5
10
15
20
25
0.3 0.6 0.9 1.3 1.6 1.8 2.12 pentyl pyridine (mM)
Bou
nd 2
pen
tylp
yrid
ine
(mol
es/m
ole
prot
ein)
Highly soluble in beverages, creating smooth mouth feel
Soymilk protein sedimentation*________________________HBC soymilk: 2% sediment
Control soymilk: 15% sediment
*3 wk storage
Soymilk protein sedimentation*________________________HBC soymilk: 2% sediment
Control soymilk: 15% sediment
*3 wk storage
Benefits of High β-Conglycinin Soy Protein in Food
Genomics
ConventionalBreeding
Crop Analytics
MolecularBreeding
Chemistry
Fully Integrated Approach: High Yielding Germplasm, Multi-faceted Approach to Traits, Screening Capabilities, and Markers