ProponentsJohn Rovic Ngo,Trisha Reyes,Lester Almarez

August2013

ProponentsJohn Rovic Ngo,Trisha Reyes,Lester Almarez

August2013

Momordica charantia L. Extract as aProphylactic Medicine for Gram-

Negative Bacteria Escherichia coli

Momordica charantia L. Extract as aProphylactic Medicine for Gram-

Negative Bacteria Escherichia coli

A Science Research Project Presented to The Faculty of Science and Technology

 

A Science Research Project Presented to The Faculty of Science and Technology

 

Abstract

• Since plants are used as therapeutic agents, the study was conducted to

evaluate the second metabolites of Momordica charantia Linn’s extract and it’s

antibacterial activities. Studies on the plant’s extract itself, 75% ethyl alcohol and

sample free-disk as the control setups using Disc Agar Diffusion Method.

• The microorganism.used is Gram Negative bacteria Escherichia coli. The maximum activity was observed against the said bacteria. The minimum zone of inhibitory concentration ranged at 10mm on microorganism. As conducted, Momordica charantia L. was observed to have antibacterial activity and can be used as medicinal purposes.

Introduction

• Bacteria are living things that are ubiquitous. Most of them won’t hurt you. Many are helpful for digestion, destroy disease-causing cells and give the body needed vitamins. But infectious bacteria can cause severe illness. They produce themselves quickly inside the body. Many emit chemicals such as toxins, which can damage tissues.One of the common bacteria that infect us is the gram-negative Escherichia coli.

• So, we, the proponents tried to find a resourceful way of medication for Antimicrobial diseases using Momordica charantia Linn extract.

Some Terms Used

• Momordica Charantia• Escherichia coli• Evaporation• Inoculation

Methodology

• Sterilization Of Materials• Extraction of Plant Material• Test Organism• Control Set-Up(s)• Inoculum Preparation• Inoculation• Application of Paper Discs With Extracts• Gathering Of Results

Gathering of Materials

Sterilization of Materials

Extraction Of Plant Material

Test Organisms

Research Paradigm

Sterilization of Materials

Extraction of Plant Material

Test Organism

Control Set-Up(s)

Inoculum PreparationInoculation

Application of Paper Disks With Extracts

Gathering of Results

Results

Legends

Disc: 10 mm diameterReactivity Rating: 0 – None (No detectable zone around or under specimen)

1 – Slight (Some malformed or degenerated cells under the specimen)

2 – Mild (Zone limited under the specimen)

3 – Moderate (Zone extends 5 to 10 mm beyond specimen)

4 – Severe (Zone extends greater than 10 mm beyond specimen)Inhibitory Activity Rating: (+++) complete, (++) partial, (+) slight and (-) negative

Results and Interpretation

• The sample, “Ampalaya Extract”, produced complete inhibitory activity (+++) with mild reactivity (2) against test organism, Escherichia coli; The sample “Alcohol”, which  served as control, had negative inhibitory activity (-) and no reactivity (0) against the test  organism, E. coli.  The sample free disc, which served as negative control, produced negative inhibitory activity (-) and no reactivity (0) against the test organism.

 

Discussion

• In this experiment, 3 set-ups were used to determine on which will be the most effective prophylactic medicine for gram –ve bacteria Escherichia coli diseases.

• From the tabulation, the “Ampalaya Extract” produced the positive result of reactivity.This shows the capabilities of Momordica charantia L.’s extract can be a new medicine for gram –ve bacteria Escherichia coli.

Conclusion

• The proponents’ research proved that the application of Momordica charantia L.As significance to be the prophylactic medicine for E. coli. 

• This concludes to the fact that the pure extract of the said plant is a good Medicine for diseases of the said bacteria.

• Therefore, the use of the extract of ampalaya can be an alternative agent for E.coli.

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THANK YOU!