mito viewer loop presentaion
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Monitoring of Tissue Energy Metabolism and Mitochondrial Function
Very New Development by
Prizmatix Ltd. ISRAEL
www.Prizmatix.com
Optical assessment of intracellular Mitochondrial Function Optical assessment of intracellular Mitochondrial Function Using NADH monitoring by the MitoViewerUsing NADH monitoring by the MitoViewer
Mito
ViewerMitochondrion
By Prizmatix Ltd.Israel www.Prizmatix.com
Mitochondrial NADH (Fluorometry)
ATP
Typical Examples:
Kidney Function
Gastrointestinal Activity
Muscle Contraction
Brain Ionic Homeostasis
Glandular Secretion
Tissue Blood Flow (LDF)
HbO
2
Hemoglobin Oxygenation (Oximetry)
Venule
Arteriole
O2
O2
O2
O2
2
O2
O2O2
O2
O2
O2
O2
O2
O2
O2
O2
O2
O2
Mitochondrial Function represents the energy balance of the tissue
Mitochondrial Function represents the energy balance of the tissue
1. Ionic Homeostasis1. Ionic Homeostasis2. Signal Conduction2. Signal Conduction3. Muscle contraction3. Muscle contraction4. Glandular Secretion4. Glandular Secretion
55 . .G-I tract and kidney G-I tract and kidney FunctionFunction
TBFTBFpOpO22HbOHbO22
Tissue energy balance between supply and demand is reflected by the mitochondria
The MitochondrionThe Mitochondrion The NADH molecule The NADH molecule
is a control marker in is a control marker in the energy the energy generation chain in generation chain in the mitochondriathe mitochondria
An increase in the An increase in the NADH levels NADH levels indicates that indicates that metabolic imbalance metabolic imbalance unfoldsunfolds
More than 1,000 papers were published on NADH monitoring related to cellular energy metabolism under
in vitro and in vivo conditions
Body metabolicimbalances
Cancer
Neuro-degenerative
DiseasesNervous System
Development
Proliferation
Therapy
Parkinson’s
Alzheimer’sHuntington
AmyotrophicLateral sclerosis
Hepatitis C
Hepatopathies
Tubulopathy
Transplantation
Traumatic brain injury
Adults
Newborns & Children
Spinal cord
Stroke & Neuronal activity
Sepsis
Intensive Care Patients
Neonatal
Adults
Liver & Kidney
Human diseases related to mitochondrial activity
Where to Monitor NADH redox state
During the last 60 years NADH was monitored almost in all organs of the body.
Our group (Mayevsky et al) had published more than 150 papers in
various animal models.
More than 1,000 papers had been published in this area of research .
77
kidney
Testis
Small Intestine
Liver
Heart
Urethra
Spinal cord
Animal
ClinicalClinicalPigs
Ischemia
NE
NE
Ischemia
NE
HypercapniaPapaverine
Ischemia
N2
NEHemorrhage AAA
ICU
Bypass
Pacing
Hypopnea
Ischemia
Drugs (Ach, NE,
vasoactive)
Compression
Ischemia
Oxygen deficiencyIschemia
NO
Drugs
TBI
HyperbariaHBO
Clinical
Activation
CO
Hemorrhage
Hypothermia
Aging
Sepsis
Epilepsy
SD
Mannitol
ICP elevation
Retraction
Anoxia
Hypoxia
Hypercapnia
Nimodipine
Ethanol
Anesthetics
UncouplerDuring operation
ICU
Brain
The UV light is helping us to shed new light into the darkness of Mitochondrial Functions
Optical assessment of intracellular Mitochondrial Function Optical assessment of intracellular Mitochondrial Function Using NADH monitoring by the MitoViewerUsing NADH monitoring by the MitoViewer
Mito
ViewerMitochondrion
By Prizmatix Ltd.Israel www.Prizmatix.com
Detailed description of the MitoViewer components
6% Oxygen Air 100% Oxygen Air
Reflectance Fluorescence NADH1212
Most of the information related to NADH monitoring appears in the following review:
Mayevsky, A. and Rogatsky,G.G. Mitochondrial function evaluated by NADH fluorescence: From animal models to human studies.
Am J Physiol, Cell Physiol 292: C615-C640 (2007)
1313
For additional information contact: Prof. Avraham Mayevsky
Prizmatix Ltd.POB 4234,Modiin-Ilite 71919, ISRAELPhone: +972-8-929-7844Fax: +972-8-929-8772www.prizmatix.com