Mike AndrewsIACUC Training and Compliance Coordinator

Office of the IACUC

We as a research community are charged by thepublic with the responsibility to assure animal welfare in exchange for the privilege of utilizing animalsin the advancement of human welfare.

IACUCAdministrator

VetReview

DMR

IACUC Committee

Approved for3 years

Amendments?Administrative

DMRCommittee PAM every

6 months

2 years, 9 monthsTime to renew!

DefinitionsIACUC – Institutional Animal Care and Use CommitteeOAR – Office of Animal Resources

PHS & NIH – Public Health Services & National Institutes of HealthOLAW – Office of Laboratory Animal WelfareAAALAC – Association for Assessment and Accreditation of Laboratory Animal Care InternationalThe Guide – The Guide to the Care and Use of Laboratory Animals

USDA – United States Department of AgricultureAPHIS – Animal and Plant Health Inspection ServicesAWA – Animal Welfare Act

Compliance – Following all regulations set forth in the PHS Policy on Humane Care and Use of Laboratory Animals, theGuide and AWA

Reportable Event – Departure from guidelines that is reported to Grantors

USDA(United States Department

Of Agriculture)

Regulatory Oversight of Animals Used in Research

PHS(Public Health Services)

AAALAC, International(Association for the Assessment andAccreditation of Laboratory Animal

Care International)

UNIVERSITY OF IOWAIACUC

(Institutional Animal Care & Use Committee)

AP or Protocol(Animal Protocol)

Investigator

NIH(National Institute Of Health)

OLAW(Office of Laboratory Animal

Welfare)

APHIS(Animal and Health Plant

Inspection Services)

AWA (Animal Welfare Act)

“The Guide” (The Guide for the Care and Use of Laboratory Animals)

USDA has announced this is the:

http://nexus.od.nih.gov/nexus/nexus.aspx?ID=473&Month=06&Year=2010

The new “Guide” is out

The IACUC and PAM are here to “guide” youthrough the new Guide:

EducateResourceConnect

Information on Animal Protocols remains up-to-date and in compliance.

Foster a teamwork environment between the laboratories and IACUC.

Allow for an exchange of information and education.

Create consistency in policies which allows consistency in research performed at the University.

Our purpose is to allow your research to proceed without interruption or jeopardy!

Uncovered cages in public areas

All cages and transport vehicles, regardless of whether they contain animals or not, must be covered when outside of Animal Resources facility.

•Animals\caging must not be visible.•Carry no more than 2 mouse/rat cages.•Must use cart for more than 2 mouse/rat cages.

Material covering the cage must be oxygen permeable. A plastic garbageis not an acceptable method!!

If your mouseis looking backat you, he isNOT covered!

Animals cannot be kept outside of the animal husbandry facilitiesfor more than 12 hours (USDA species) or 24 hours (rodents)without IACUC approval.

This is a federal requirement!!!This request requires the convening of the full IACUC before approval can be granted!!

Animals being housed in laboratories without prior approval

You are responsible for cleaning up after your animals!

University custodians are notallowed to handle animal waste.

Not cleaning up animal waste

• Animals should remain in original housing cage.• DO NOT COMBINE animals from any other cage.

• Filter top must remain on cage.• Gently insert tube through water hole flap.

• Turn on CO2 gas to Level 15.• Maintain gas flow until breathing stops, AND• Continue gas flow for 1 additional minute.

• Turn off CO2 gas.

DO NOTTOUCH!

Proper Use of CO2 inEuthanasia Procedures

• Remove tube.• Leave animals in cage with CO2 for at least 5 more minutes.

Filter top mustremain in place

Flap mustremain closed

Flap mustremain closed

• Perform confirmation of death as described in the approved Animal Protocol.

• After confirmation of death has been performed, place animalsin plastic bag.

• Tie a knot in the bag.• Place bag into a red container in the cooler.

Confirmation of death must be performed on every animal

It is required that a confirmation of death be performedon every animal being euthanized. The method of confirmation must be identified in the approved Animal Protocol.

Examples:

Animals under 500 gm:• Cervical dislocation• Decapitation• 15 minutes in a CO2 charged chamber post-respiratory arrest• Thoracotomy• Vital tissue harvest (must include brain, heart and/or lungs)Animals over 500 gm:• Bilateral thoracotomy• Vital tissue harvest (must include brain, heart and/or lungs)

•No photos are allowed in the animal housing facility with a cell phone or any other camera, except with written authorization from the Office of Animal Resources.

Misuse of animal access:Cameras/cell phones

•Do not allow people you do not know personally into the facilities.•Do not admit other people into the animal facility or animal rooms.•You are authorized only in animal rooms where your animals are housed.

Misuse of animal access:Entering facilities

All persons performing survival surgeries must be observed performing

one surgery

The Definition of Surgery:

Disruption of any integumentary surface of a living animal by any means other than a hypodermic needle, biopsy needle, ear punch, or tail snip is considered surgery. 

Surgical reports must be maintained!Surgical records are a record of whatactually happened, not a reiterationof what is stated in the Animal Protocol•Date•Animal Protocol number•Animal ID•Procedure•Anesthetic agents•Time of induction•Time of recovery•Date(s) and time(s) of analgesia•Post-op monitoring•Date when sutures are removed or are no longer present

If it’s not written down,it didn’t happen!!

Performing procedures onThe wrong animal orAnimal Protocol (AP)

Before performing any procedures, double-check the AP number on the animal cage to the approved AP to verify that the procedure is approved under that AP number.

Performing unapprovedprocedures:

Before performing any procedures, double-check that the procedure to be performed is approved under that AP number.

A description of the procedure must be approved on an AP before it can be performed.

Unapproved Personnel:

Before performing any procedures, double-check that the person who is about to perform a procedure is listed on that AP number.

It is required that each person working with animals must be listed on each AP.

You ARE NOT approved if:

•You are listed on one approved AP, but are handling the animals approved under another•Your PI is listed on an approved AP, but you are not •An amendment has been sent in, but not yet approved

IncorrectlyTransferring AnimalsBetween PIs

Animals transferred to a different PI and AP cannot be used until transfer has been approved!!!

Signatures from both labs must be obtained.

Originating lab Receiving lab

1. Originating PI/lab tech labels each cage to be transferred with a Purple Transfer Card (obtained from the Area Supervisor).

2. Originating PI/lab tech fills out the top section of a yellow mouse slip for “Transfer Mice.” Originating lab must sign transfer slip.

3. Receiving PI/lab tech must fill out the bottom section of the transfer slip. Receiving PI/lab must sign the transfer slip.

4. After both signatures have been obtained:1. Give to area Supervisor, OR2. Give to area Caretaker, OR3. Leave slip on one of the labeled cages

to be transferred.

How to Transfer Animals Between PI/Labs

Receiving PI/lab CANNOT touch, use or move the transferred animals until the Receiving PI/lab has received an email from OAR stating that the transfer has been approved.

1. PI/lab fills out yellow mouse slip for “Transfer Mice.”

2. If OAR is moving the animals:1. Label cages with a Purple Transfer

Card (obtained from the area Supervisor) to be moved by OAR

2. Leave slip on one of the labeled cages to be moved, OR

3. If the PI/lab is moving the animals:1. Obtain a signature from the area

Supervisor/Caretaker (to ensure the building/room change is suitable and available)

2. PI/lab can then move the cages3. This option is only available when a

car or van is not required for the move

How to Transfer Animals Between Rooms

To summarize:

Questions

Comments?

Concerns?