microbial methanol synthesis team 3: robert clifford, patricia firmin, gen liang, rooma raza

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Microbial Methanol Synthesis Team 3: Robert Clifford, Patricia Firmin, Gen Liang, Rooma Raza

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Microbial Methanol Synthesis

Team 3: Robert Clifford, Patricia Firmin, Gen Liang, Rooma Raza

Methanotrophs

Where Do These Bacteria Live?Wetlands Sewage

Lake Basin Bogs

Methanotroph Groupings

Group I: Ribulose Monophosphate Cycle Group II: Serine Cycle

Inhibit Enzyme MDH

Optimize Enzyme MMO

Methane MonooxygenasepMMO – Particulate Methane Monoxygenase

sMMO – Soluble Methane Monoxygenase

Advantages of using ammonia-oxidizing bacteria (AOB)

• Oxidize methane to methanol via the nonspecific action of the

enzyme ammonia monooxygenase

• Contaminants such as moisture and CO2 do not post a limitation for

biological conversion

• Can utilize the CO2 contained in gas mixtures for cell synthesis

Metabolism of AOB

Methanol Production by AOB

• Methanol production rate varies for conditions and performance

• Maximum specific productivity is 0.82 mg methanol/ mg biomass

(COD)/d

Industrial Challenges

• Microorganisms are limited.

• Inhibition on cell growth by H2S when methane in biogas

is used.

• High-cost electron donors required for conversions

• Gas-Liquid Mass transfer limitations

• NH3 may inhibit the growth of microorganisms including

methanotrophs

Changes in the reactor design by using Trickling biofilters for enhanced methane

supply

Methanotrophic strains from AD systems

Genetic engineering of ANME

Microbial electrosynthesis

Potential Strategies

Outlook for Microbial Conversion

Batch and Semi-batch Production

Membrane Semi-batch Process

Methanol Production Results

References

• Fei, Q. G., Michael. Tao, Ling. Laurens, Lieve. Dowe, Nancy. Pienk, Philip T.os. (2014). Bioconversion of natural gas to liquid fuel: Opportunities and challenges. 32(3), 596–614. doi: 10.1016/j.biotechadv.2014.03.011

• Ge, X., Yang, L., Sheets, J. P., Yu, Z., & Li, Y. (2014). Biological conversion of methane to liquid fuels: Status and opportunities. Biotechnology Advances, 32, 1460-1475. doi: - http://dx.doi.org/10.1016/j.biotechadv.2014.09.004

• Pen, N. S., L. Belleville, M.-P. Sanchez, J. Charmette, C. Paolucci-Jeanjean, D. (2014). An innovative membrane bioreactor for methane biohydroxylation. Bioresource Technology, 174, 42–52. doi: 10.1016/j.biortech.2014.10.001

• Thorn, G. J. S. (2005). Development of an Immobilized Nitrosomonas europaea Bioreactor for the Production of Methanol from Methane - thesis_fulltext.pdf. Department of Chemical Process Engineering. University of Canterbury. Retrieved from

http://ir.canterbury.ac.nz/bitstream/10092/1867/1/thesis_fulltext.pdf