methamphetamine &...

Rapid and Accurate Chiral Separation

METHAMPHETAMINE & AMPHETAMINE Enantiomers from Urine

New Lux 3 µm AMP Column• High Efficiency

• LC/MS/MS Compatible

• Quality Tested for METH/AMP Chiral Analysis

Polysaccharide Chiral LC and SFCDependable. Scalable. A�ordable.

Chiral LC Columns

Phenomenex l WEB: www.phenomenex.com

A Trusted and Experienced Solution Provider For over 30 years Phenomenex has developed separation solutions for the forensics and toxicology industries. This strong focus, combined with innovative manufacturing, packing and sorbent technologies has now lead to the development of a novel LC column specifically designed to help you overcome the challenging drug separation of amphetamine and methamphetamine enantiomers.

D-Methamphetamine

S(+)-Methamphetamine

HN

D-Amphetamine

S(+)-Amphetamine

L-Amphetamine

R(-)-Amphetamine

Designed and Tested for Chiral Separation of Amphetamine and Methamphetamine Enantiomers

Lux 3 µm AMP is a unique LC media that was specifically developed for the chiral analysis of amphetamine and substituted amphetamines, including methamphetamine. This type of separation is invaluable for forensics, toxicology, and clinical research labs that need to analyze and confirm which enantiomers are found in samples to differentiate between therapeutic use and abuse.

Lux 3 µm AMPL-Methamphetamine

R(-)-Methamphetamine

HN

Polysaccharide Chiral LC and SFCDependable. Scalable. A�ordable.

Chiral LC Columns


0.00

5.00e5

1.00e6

1.50e6

2.00e6

2.50e6

3.00e6

3.50e6

4.00e6

4.50e6

5.00e6

5.50e6

6.00e6

6.50e6

7.00e6

7.50e6

8.00e6

8.50e6

9.00e6

9.50e6

1.00e71.03e7

0.5 1.0 1.5 2.0 2.5 3.0 3.5 min

0 2 4 6 8 10 12 min767

1.0e6

2.0e6

3.0e6

4.0e6

5.0e6

6.0e6

1

2

3

4

Reversed Phase Screening and Presumptive TestingThe high performance and versatility of the Kinetex® 2.6 µm Biphenyl core-shell column makes it an excellent choice for a rapid reversed phase separation of multi-class screens like illicit and abused drugs from urine samples.

Comparative separations may not be representative of all applications.

Inte

nsity

, cps

Inte

nsity

, cps

Column: Kinetex 2.6 µm BiphenylDimension: 30 x 2.1 mm

Part No.: 00A-4622-ANSecurityGuard™

ULTRA Cartridge:

AJ0-9209

SecurityGuard™ Holder:

AJ0-9000

Mobile Phase: A: 10 mM Ammonium Formate in Water (pH unadjusted)

B: 0.1 % Formic Acid in MethanolGradient: Time (min)

0.00.22.52.93.03.5

% B101090901010

Flow Rate: 0.4 mL/minTemperature: 40 ˚C

Injection Volume: 25 µLDetection: MS/MS (SCIEX 6500 QTRAP®)

Column: Lux 3 µm AMPDimension: 150 x 3.0 mm

Part No.: 00F-4751-Y0Mobile Phase: A: 5 mM Ammonium Bicarbon-

ate, adjusted to pH 11 with Ammonium Hydroxide

B: MethanolFlow Rate: 0.42 mL/min

Gradient: Time (min)0101116

16.3

% B6060959560

Temperature: AmbientDetection: MS/MS (SCIEX 4500 QTRAP®)

Sample: 1. R(-)-Amphetamine2. R(-)-Methamphetamine3. S(+)-Amphetamine4. S(+)-Methamphetamine

Sample:Positive Mode (ESI+) 1. 6-MAM 2. 7-Aminoclonazepam 3. 3 alpha- hydroxyalprazolam 4. Alprazolam 5. Amitriptyline 6. Amphetamine 7. Benzoylecgonine 8. Buprenorphine 9. Carisoprodol 10. Citalopram 11. Codeine 12. Cotinine 13. Diazepam 14. EDDP 15. Fentanyl 16. Fluoxetine 17. Gabapentin 18. Hydrocodone 19. Hydromorphone 20. Imipramine 21. Lorazepam 22. MDMA 23. Meperidine 24. Meprobamate

25. Methadone 26. Methamphetamine 27. Methylphenidate 28. Morphine 29. Naloxone 30. Norbuprenorphine 31. Nordiazepam 32. Norfentanyl 33. Norhydrocodone 34. Noroxycodone 35. Normorphine 36. Nortriptyline 37. O-Desmethyltramadol 38. Oxycodone 39. Oxymorphone 40. Paroxetine 41. PCP 42. Pregabalin43. Sertraline44. Tapentadol45. Temazepam46. Tramadol47. Zolpidem48. Zolpidem-4- carboxylic Acid

Negative Mode (ESI-) 1. Butalbital 2. Phenobarbital 3. Secobarbital 4. THC-COOH

AP

P ID

23560A

PP

ID 23631

Kinetex 2.6 µm BiphenylTIC of ESI+ Panel

Methamphetamine

Amphetamine

Lux 3 µm AMP

Inte

nsity

, cps

Kinetex 2.6 µm Biphenyl

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 min0.0

2000.04000.06000.08000.0

1.0e41.2e41.4e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e4

AP

P ID

23566

Enantiomeric Definitive TestingOnce presence of amphetamine or methamphetamine has been determined, enantiomeric confirmation can then easily be achieved with a Lux® 3 µm AMP column.

TIC of ESI- Panel


Not Affected by Common Interferences Another excellent benefit of the Lux® 3 µm AMP is that its separation of amphetamine and methamphetamine enantiomers isn’t affected by common therapeutics and ingredients such as those seen below. In addition, the separation power of the Lux AMP column can also help with resolution between enantiomers of substituted amphetamines.

1-Minute β-Glucuronidase Removal To remove β-Glucuronidase before it can build up and foul your LC instrumentation, Mass Spectrometer, and column, we recommend the use of Beta-Gone. This novel sorbent offers a simple, fast, and highly targeted method to remove β-Glucuronidase from hydrolyzed urine samples prior to analysis. Within 1 minute, with no necessary method development, your samples will be ready for analysis.

www.phenomenex.com/beta-gone


Inte

nsity

, cps

Column: Lux 3 μm AMPDimension: 150 x 3.0 mm

Part No.: 00F-4751-Y0Mobile Phase: A: 5 mM Ammonium Bicarbon-

ate, adjusted to pH 11 with Ammonium Hydroxide

B: MethanolFlow Rate: 0.42 mL/min

Gradient: Time (min)0101116

16.3

% B6060959560

Temperature: AmbientDetection: MS/MS (SCIEX 4500 QTRAP®)

1. 1S,2R(+)-Ephedrine2. R,R(-)-Pseudoephedrine3. S,S(+)-Pseudoephedrine4. 1R,2S(-)-Ephedrine5. R(-)-Amphetamine6. R(-)-Methamphetamine7. S(+)-Amphetamine8. S(+)-Methamphetamine9. Phentermine10. MDMA11. MDMA

Compounds included in this interference study but not illustrated chromatographically: acetaminophenaspirin(±)-chlorpheniraminecaffeinediphenhydraminedextromethorphanibuprofen(±)-MDA(±)-MDEAphenylephrinenorephedrine

Sample:Positive Mode (ESI+) 1. 6-MAM 2. 7-Aminoclonazepam 3. 3 alpha- hydroxyalprazolam 4. Alprazolam 5. Amitriptyline 6. Amphetamine 7. Benzoylecgonine 8. Buprenorphine 9. Carisoprodol 10. Citalopram 11. Codeine 12. Cotinine 13. Diazepam 14. EDDP 15. Fentanyl 16. Fluoxetine 17. Gabapentin 18. Hydrocodone 19. Hydromorphone 20. Imipramine 21. Lorazepam 22. MDMA 23. Meperidine 24. Meprobamate

25. Methadone 26. Methamphetamine 27. Methylphenidate 28. Morphine 29. Naloxone 30. Norbuprenorphine 31. Nordiazepam 32. Norfentanyl 33. Norhydrocodone 34. Noroxycodone 35. Normorphine 36. Nortriptyline 37. O-Desmethyltramadol 38. Oxycodone 39. Oxymorphone 40. Paroxetine 41. PCP 42. Pregabalin43. Sertraline44. Tapentadol45. Temazepam46. Tramadol47. Zolpidem48. Zolpidem-4- carboxylic Acid

Negative Mode (ESI-) 1. Butalbital 2. Phenobarbital 3. Secobarbital 4. THC-COOH

0 2 4 6 8 10

1 2 3-45

6

7

89

1011

12 14 16 18 min0

1.0e6

2.0e6

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

7.8e6

AP

P ID

23640

β-Glucuronidase Removal Products


0.0

2.0e4

4.0e4

6.0e4

8.0e4

1.0e5

1.2e5

1.4e5

1.6e5

1.8e5

2.0e5

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 min


Exceptional ReliabilityLux® 3 μm AMP media and columns are designed to be consistent and incredibly accurate tools for your amphetamine and methamphetamine analysis. Each batch is specifically tested by LC/MS for the analysis of amphetamine and methamphetamine, and columns are quality tested to ensure dependability and reproducibility.

Amphetamine Enantiomers Methamphetamine Enantiomers

AP

P ID

23629

Excellent Lifetime

Column: Lux 3 µm AMPDimension: 150 x 3.0 mm

Part No.: 00F-4751-Y0Mobile Phase: A: 5 mM Ammonium Bicarbonate, adjusted

to pH 11 with Ammonium HydroxideB: Methanol

Flow Rate: 0.42 mL/minGradient: Time (min)

0101113

13.1

% B6060959560

Temperature: 22 °CSample: 1. Ephedrine

2. Pseudoephedrine3. R(-)-Amphetamine4. S(+)-Amphetamine5. R(-)-Methamphetamine6. S(+)-Methamphetamine

% o

f Ini

tial R

eten

tion

Tim

e

R(-)-Amphetamine R(-)-MethamphetamineS(+)-Amphetamine S(+)-Methamphetamine

Injection Count 1 350 7000

20

40

60

80

100

0.01.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.4e5

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 min

AP

P ID

23628

Resolution

Batch A: 3.38 Batch B: 3.04

Resolution


Ephedrine and Pseudoephedrine

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 min0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e5

AP

P ID

23630

Resolution


R(-)-Amphetamine R(-)-Methamphetamine

1S,2R(+)-Ephedrine

S(+)-Amphetamine

S(+)-Methamphetamine

S,S(+)- Pseudoephedrine


If Phenomenex products in this brochure do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, return the product with comparative data within 45 days for a FULL REFUND.

Ordering Information

Rapid and Accurate Chiral Separation

METHAMPHETAMINE & AMPHETAMINE Enantiomers from Urine

NEW

BR

4244

0416

_W

Lux® 3 µm Analytical ColumnsSecurityGuard™

ULTRA Cartridges‡

Phase 150 x 3.0 150 x 4.6 3/pk 3/pk

AMP 00F-4751-Y0 00F-4751-E0 AJ0-9510 AJ0-9509

for 3.0 mm ID for 4.6 mm ID

Kinetex® 2.6 µm Analytical ColumnsSecurityGuard™

ULTRA Cartridges‡

Phase 30 x 2.1 50 x 2.1 50 x 3.0 3/pk 3/pk

Biphenyl 00A-4622-AN 00B-4622-AN 00B-4622-Y0 AJ0-9209 AJ0-9208

for 2.1 mm ID for 3.0 mm ID

‡SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000

β-Gone™ β-Glucuronidase Removal Products

Part No. Description Unit Price

8B-S139-TAK 1 mL Tubes, Recombinant Enzyme 100/Box

8B-S322-DAK 1 mL Tubes, Non-Recombinant Enzyme 100/Box

8E-S139-TGA 96-Well Plate, Recombinant Enzyme 1/Box

8E-S322-DGA 96-Well Plate, Non-Recombinant Enzyme 1/Box

Verex™ Certified Mass Spec Quality Vials

Description 1000/pk Price

Cert+ MSQ Vial Kit, 9 mm, 2 mL Clear w/ Patch + MSQ PTFE/Silicone

AR0-992A-13-M

Cert+ MSQ Vial Kit, 9 mm, 2 mL Amber w/ Patch + MSQ PTFE/Silicone

AR0-992B-13-M

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

Trademarks Phenomenex, Lux, and Kinetex are registered trademarks of Phenomenex. β-Gone, Verex, and SecurityGuard are trademarks of Phenomenex. QTRAP is a registered trademark and API 4000 is a trademark of AB SCIEX Pte. Ltd. AB SCIEX is being used under license.


© 2016 Phenomenex, Inc. All rights reserved.

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