‍‍‍‍ |   | 

Education8/1998-5/2003 | NORTH CAROLINA STATE UNIVERSITY

· B.‍‍S.‍‍in‍‍Biochemistry,‍‍Minor‍‍in‍‍Genetics

7/2009 | ASCP CERTIFICATION IN CYTOGENETICS

· ASCP‍‍certification‍‍#:‍‍2675‍‍(current)

1/2015-CURRENT | IOWA STATE UNIVERSITY

· Plant‍‍Breeding

Professional Memberships

· American‍‍Society‍‍for‍‍Biochemistry‍‍and‍‍Molecular‍‍Biology· American‍‍Society‍‍for‍‍Clinical‍‍Pathology

Skills & AbilitiesMOLECULAR BIOLOGY/GENETICS

· Experienced‍‍in‍‍DNA/RNA/protein‍‍isolation,‍‍gene‍‍cloning,‍‍sequence‍‍analysis,‍‍and‍‍transformation‍‍into‍‍E.‍‍coli‍‍and‍‍Agrobacterium‍‍via‍‍electroporation‍‍or‍‍heat-shock.‍‍

· Technique‍‍requires‍‍working‍‍knowledge‍‍of‍‍PCR,‍‍restriction‍‍enzyme‍‍digestion,‍‍Gibson‍‍cloning.‍‍

· Specialized‍‍work‍‍includes‍‍Southern‍‍Blotting‍‍and‍‍analysis,‍‍hybridoma‍‍creation,‍‍protein‍‍purification‍‍and‍‍analysis‍‍by‍‍spectroscopy‍‍and‍‍ELISA.‍‍

· Demonstrated‍‍experience‍‍with‍‍cell/‍‍tissue‍‍isolation‍‍and‍‍culture,‍‍cytogenetic‍‍staining‍‍and‍‍analysis,‍‍FISH‍‍and‍‍FISH‍‍analysis.

PROJECT AND LAB MANAGEMENT

· Proficient‍‍at‍‍ordering,‍‍stocking,‍‍managing‍‍and‍‍tracking‍‍limited‍‍laboratory‍‍and‍‍student‍‍purchase‍‍budgets.

· Facilitated‍‍training‍‍of‍‍new‍‍lab‍‍personnel.

· Performed‍‍environmental‍‍and‍‍radioactive‍‍lab‍‍safety‍‍inspections‍‍and‍‍documentation‍‍to‍‍be‍‍in‍‍compliance‍‍with‍‍Federal‍‍and‍‍State‍‍regulations.

· Wrote‍‍and‍‍implemented‍‍SOPs‍‍and‍‍Work‍‍Instructions‍‍for‍‍new‍‍and‍‍existing‍‍lab‍‍processes.

· Responsible‍‍for‍‍researching‍‍new‍‍equipment‍‍cost‍‍and‍‍function.

· Efficient‍‍at‍‍negotiatating‍‍vendor‍‍contracts‍‍and‍‍obtaining‍‍competitive‍‍quotes.

Publications· ‍‍McCann ML,‍‍Owens‍‍EL,‍‍Sadeghpour‍‍A,‍‍Phillips‍‍KK,‍‍and‍‍Buchanan‍‍P‍‍(2010)‍‍Prenatal‍‍evaluation‍‍of‍‍agenesis‍‍of‍‍

the‍‍corpus‍‍callosum‍‍using‍‍high‍‍density‍‍scan‍‍microarray.‍‍‍‍J‍‍Assoc‍‍of‍‍Genet‍‍Technol‍‍‍‍A36,p.140

ExperienceR & D SPECIALIST, VECTORING | BAYER CROPSCIENCE | 11/2012-CURRENT

· Gene‍‍cloning,‍‍sequencing,‍‍and‍‍transformation‍‍into‍‍E.‍‍coli‍‍and‍‍Agrobacterium‍‍gene‍‍optimization,‍‍Vectoring‍‍Platform‍‍validation.‍‍

· QC‍‍of‍‍submitted‍‍constructs‍‍using‍‍restriction‍‍digest,‍‍PCR,‍‍and‍‍evaluation‍‍on‍‍NextGen‍‍Sequencing‍‍results.

· Responsible‍‍for‍‍luciferase‍‍expression‍‍testing‍‍for‍‍stable‍‍versus‍‍transient‍‍transformation,‍‍and‍‍representation‍‍or‍‍status‍‍updates‍‍for‍‍other‍‍groups.

· Experienced‍‍in‍‍the‍‍use‍‍of‍‍DNA‍‍purification‍‍from‍‍culture,‍‍Southern‍‍Blotting‍‍and‍‍analysis,‍‍use‍‍of‍‍VectorNTi,‍‍Sequencher‍‍and‍‍Geneious‍‍software‍‍tools‍‍for‍‍sequencing‍‍analysis,‍‍and‍‍constant‍‍electronic‍‍updates‍‍to‍‍archive‍‍databases‍‍for‍‍compliance‍‍with‍‍safety‍‍and‍‍stewardship‍‍standards.

· Supported‍‍with‍‍other‍‍special‍‍projects‍‍to‍‍establish‍‍tissue‍‍culture‍‍line‍‍and‍‍develop‍‍RNA‍‍constructs.

CONTRACT MOLECULAR BIOLOGIST FOR BAYER CROPSCIENCE | AEROTEK SCIENTIFIC | 6/2011-11/2012

· Performed‍‍gene‍‍cloning,‍‍sequencing,‍‍and‍‍transformation‍‍into‍‍E.‍‍coli‍‍and‍‍Agrobacterium,‍‍and‍‍constant‍‍electronic‍‍updates‍‍to‍‍archive‍‍databases‍‍for‍‍compliance‍‍with‍‍safety‍‍and‍‍stewardship‍‍standards.

· Responsible‍‍for‍‍soybean‍‍DNA‍‍isolation‍‍using‍‍KingFisher‍‍automation,‍‍quantification‍‍by‍‍gel‍‍electrophoresis,‍‍and‍‍protein‍‍detection‍‍using‍‍lateral‍‍flow‍‍strips‍‍for‍‍cotton‍‍and‍‍soybean‍‍seed‍‍samples.

CYTOGENETIC TECHNOLOGIST, CG(ASCP)| ALERE GENETICS (GENECARE) | 7/2009-4/2011

CYTOGENETIC TECHNOLOGIST, CG(ASCP)| GENECARE MEDICAL GENETICS CENTER | 2/2008-7/2009

· Clinical‍‍diagnostic‍‍position‍‍involving‍‍cell‍‍culture‍‍and‍‍harvest‍‍of‍‍human‍‍blood,‍‍bone‍‍marrow,‍‍and‍‍amniotic‍‍fluid‍‍samples‍‍for‍‍microscopic‍‍analysis‍‍of‍‍chromosome‍‍abnormalities.‍‍

· Performed‍‍FISH‍‍analysis‍‍of‍‍samples‍‍for‍‍discrepancies‍‍in‍‍number‍‍of‍‍chromosomes,‍‍and‍‍for‍‍presence‍‍of‍‍common‍‍microdeletions‍‍or‍‍translocations.

· Running‍‍of‍‍wet‍‍and‍‍dry‍‍blood‍‍samples‍‍for‍‍prenatal‍‍screening

· Accessioning‍‍of‍‍samples‍‍received,‍‍generation‍‍of‍‍patients‍‍charts,‍‍and‍‍logistics‍‍for‍‍lab

· Cross-trained‍‍in‍‍patient‍‍data‍‍entry‍‍to‍‍aid‍‍billing‍‍department.

FORESTRY TECHNICIAN (SUMMER SEASONAL POSITION)| NORTH CAROLINA STATE UNIVERSITY | 6/2007-8/2007

· Prepared‍‍cuttings‍‍from‍‍genetically‍‍diverse‍‍group‍‍of‍‍pine‍‍trees‍‍for‍‍testing‍‍resistance‍‍to‍‍a‍‍certain‍‍type‍‍of‍‍fungus,‍‍updated‍‍survival‍‍database.

RESEARCH SPECIALIST| NORTH CAROLINA STATE UNIVERSITY | 3/2006-5/2007

· Performed‍‍cloning‍‍of‍‍genes‍‍related‍‍to‍‍removal‍‍of‍‍reactive‍‍oxygen‍‍species‍‍in‍‍bacteria‍‍for‍‍expression‍‍in‍‍other‍‍bacteria.

ASSOCIATE SCIENTIST| CELLZDIRECT, INC. | 6/2005-3/2006

· Isolated‍‍hepatocytes‍‍from‍‍mammalian‍‍tissue.‍‍Cells‍‍were‍‍plated‍‍and‍‍sold‍‍for‍‍pharmaceutical‍‍testing‍‍for‍‍clients‍‍on‍‍and‍‍off‍‍site.

· Cross-trained‍‍in‍‍logistics‍‍and‍‍sales‍‍order‍‍data‍‍entry‍‍to‍‍provide‍‍assistance‍‍to‍‍other‍‍groups.

RESEARCH TECHNICIAN II| DUKE UNIVERSITY MEDICAL CENTER | 1/2004-5/2005

· Created‍‍and‍‍maintained‍‍hybridoma‍‍cell‍‍lines‍‍from‍‍rodent‍‍lymphocytes,‍‍used‍‍high‍‍density‍‍cell‍‍culture‍‍techniques‍‍to‍‍produce‍‍antibodies‍‍that‍‍were‍‍purified,‍‍tested‍‍and‍‍labeled‍‍for‍‍use‍‍in‍‍staining,‍‍flow‍‍cytometry,‍‍and‍‍ELISA.

INTERNSHIP| SYNGENTA BIOTECHNOLOGY | SUMMER 2002

· Cloned‍‍maize‍‍genes‍‍involved‍‍with‍‍embryo‍‍development,‍‍extracted‍‍DNA‍‍and‍‍RNA‍‍for‍‍Southern/Northern‍‍blotting‍‍and‍‍other‍‍cytogenetic‍‍studies,‍‍and‍‍analyzed‍‍soybean‍‍seed‍‍lines‍‍for‍‍sugar‍‍compositions‍‍using‍‍GC/MS.

Page‍‍2