MCB 151

Spring 2015

Course Information

MCB 151 Website: http://www.life.uiuc.edu/mcb/151/

Login: NetID

Password: AD password

syllabus

Required Materials

• Students concurrently enrolled in MCB 150 should purchase the textbook package required for that course. MCB 151 will only use the textbook portion of the package, but the other items will be used in MCB 150 and it is the most cost effective option. This option would include a custom textbook for MCB 150/151, access to Mastering Biology, and a Practicing Biology workbook. The ISBN for this package is: 9781269378208

• For students that have previously taken MCB 150, you will only need to purchase the custom textbook and its ISBN is: 9781269517027

• Laboratory Exercises for MCB 151

• Biology Laboratory Notebook (carbonless)

Add, Drop, Section Change

• Students may use the UI–Integrate Self-Service System to add or change MCB 151 laboratory sections before 5:00 PM, Monday, February 2, 2015.

• Friday, March 13 is the last day to drop the course or to elect the Credit/No Credit option. Students may use the UI-Integrate Self-Service System to drop MCB 151 until this deadline.

Map to Davenport

Course Grading(1000 points total)

Pre-Laboratory Exercises 8 sets @ 7 points each, 52 points (drop 4 points)

Post-Laboratory Exercises* 9 sets @ 15 points each, 125 points (drop 10 points)

Laboratory Reports 7 @ 75 points each, 473 points (drop 52 points)

Laboratory Exams 2 @ 100 points each

Final Exam 1 @ 150 points

*Only students that are present and on time are eligible to complete the Post-Lab Exercise and Lab Report for that particular week. If you are absent/late, you may complete the Post-Lab Exercise for your own benefit (i.e. preparation for exams), but you will NOT earn points for it. If you are absent/late, you are not eligible to complete the Lab Report for that particular week and you may not be eligible to submit the previous weeks Lab Report.

Reading Assignments

The Reading Assignment for each week can be found on the course web site under the Laboratory Exercises link. These readings ARE testable, though much of the exam material will come from the lab

manual and lab experience.

http://www.life.illinois.edu/mcb/151/

LON-CAPA

• You will have Pre/Post Lab Assignments each week. Please make sure to follow the directions on pgs. I-11 through I-13 of your lab manual.

LON-CAPA

http://police.illinois.edu/emergencyplanning/

In an emergency in this building, we’ll have three choices: RUN (get out), HIDE (find a safe place to stay inside), or FIGHT (with anything available to increase our odds for survival).

First, take a few minutes this week and learn the different ways to leave this building. If there’s ever a fire alarm or something like that, you’ll know how to get out, and you’ll be able to help others get out too.

Second, if there’s severe weather and leaving isn’t a good option, go to a low level in the middle of the building, away from windows.

If there’s a security threat, such as an active shooter, we’ll RUN out of the building if we can do it safely or we will HIDE by finding a safe place where the threat cannot see us. We will lock or barricade the door and we will be as quiet as possible, which includes placing our cell phones on silent. We will not leave our area of safety until we receive an Illini-Alert that advises us it is safe to do so. If we cannot run out of the building safely or we cannot find a place to hide, we must be prepared to fight with anything we have available in order to survive. Remember, RUN away or HIDE if you can, FIGHT if you have no other option.

Finally, if you sign up for emergency text messages at emergency.illinois.edu, you’ll receive information from the police and administration during these types of situations.

If you have any questions, go to police.illinois.edu, or call 217-333-1216.

Emergencies in the lab

• Eye Wash

• Fire Extinguishers

• Fire Alarms

• First Aid

Course Coordinator

Melissa Reedy

Course Coordinator MCB 150/151

Office: 208 Noyes Lab

Phone: 265-6379

Email: murray@illinois.edu

Office hours: By appointment

Exercise 1

Introducti

on:

Lab Tech

niques and S

afety

Basic S

kills

Laboratory Safety

Bacteria: Wear gloves Wash you hands before leaving the lab Notify your TA of any spills or contamination

Chemicals: Always handle reagents with caution

UV Light: Never look at UV lamps Wear protective gloves

Laboratory Techniques

Clean up: each student is responsible for cleaning up after every experiment

Labels: always label your experimental work with your name and section, b/c we share incubators, refrigerators, water baths, etc.

Pipetting: always review the proper use of pipettes before beginning experiments

Centrifuges: used to spin down and separate

Exercise 1B:Basic Laboratory Skills

,

Goals

• Understand and manipulate exponential numbers.• Become familiar with the units used for small

concentrations and volumes and be able to make conversions between them.

• Figure out concentrations of solutions or cell suspensions after dilution.

• Understand what is meant by pH and know how to calculate the pH of a solution.

• Understand how buffers work.

Exponential Numbers

• Every number can be expressed as the product of two numbers, the second one being some power of ten. For example, the number 100 can be expressed as

10 X 10 = 102

The same rules are used to express numbers less than one.

10-2 = 1/10 X 1/10 = 1/100

Units of Measurement

Common units

Mass 1 gram (g) = 103 milligrams (mg)

= 106 micrograms (µg)= 109 nanograms (ng)

Volume 1 liter (l) = 103 milliliters (ml)

= 106 microliters (µl)1 ml = 1 cubic centimeter

Length 1 meter (m) = 103 millimeters (mm)

= 106 micrometers (µm)= 109 nanometers (nm)

Dilutions

C1V1 = C2V2

Example: Given a 1 M stock solution of Tris buffer prepare 10 ml of 0.01 M Tris.

Solution: The question you need to answer is "what volume of 1 M Tris do you need to dilute to give a total volume of 10 ml of 0.01 M Tris". Thus,

you know C1, C2, and V2.

(? ml x 1 M) = (10 ml x 0.01 M)

? ml = 10 ml x 0.01 M

(1 M)

Answer: 0.1 ml

Dilution Factor

Dilution = 1/100 Dilution Factor: 100

Dilution = 1/n Dilution Factor: n

Serial Dilution: product of the individual dilutions

1/102 x 1/10 x 1/103 = 1/106 = 10-6

Aqueous Solutions

An aqueous solution is one in which water is the solvent.

Water properties:

High surface tension

High boiling point

Good Solvent

Acids, Bases, pH and Buffer

Acids/Bases:Bronsted Definition: an acid is a molecule that donates

protons and a base is a molecule that accepts protons

pH: Dependent upon the concentrations of H+ and OH- ions

Buffers:A molecule that allows the solutions to resist changes in

the hydrogen ion concentration

Assignments for next week

• Post Lab 1 and Pre Lab 2 are assigned today and due before your lab meets again in LON-CAPA.

• You must come to lab next week with your pre lab write up complete for Exercise 2A and 2B• Purpose• Procedure/Protocol