M A X I M I Z E S C I E N C E F O R L I F E

2008 CATALOG

bioo scientific intRoDUction ............................... 1

fooD & feeD sAfetY oVeRVieW .............................. 2

fooD & feeD sAfetY PRoDUcts ............................. 3

fooD & feeD sAfetY PRoDUct ListinG .................. 4

fooD & feeD sAfetY feAtURe PRoDUcts .............. 5

ReseARcH & PRecLinicAL testinG oVeRVieW ........ 6

ReseARcH & PRecLinicAL testinG PRoDUcts ....... 7

cYtoKine AssAY ListinG .......................................... 8

AntiboDY ListinG ..................................................... 9

ReseARcH & PRecLinicAL feAtURe PRoDUcts .....10

seRVices ...................................................................12

cUstomeR ReVieWs & contAct infoRmAtion .......13

TA B L E O F C O N T E N T S

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bioo scientific corporation (bioo) is a biotechnology and health care company headquartered in Austin, texas. our focus is on maximizing science with a direct impact on life. our products are being sold in over 40 countries under the trademarks MaxSignal™ and MaxSuppressor™.

BIOO Operates In fOur cOre areas

1. fooD AnD feeD sAfetY testinG

2. ReseARcH AnD PRecLinicAL

testinG PRoDUcts

3. bioo seRVices

4. DRUG DeVeLoPment

M A X I M I Z E S C I E N C E F O R L I F E

Our gOal is to become a leader in the discovery, development, manufacture and marketing of effective and innovative testing, diagnosis and healthcare solutions that address the challenges facing patients and society in general. our corporate values lie in the highest of ethical standards, with an ultimate focus on our customers. bioo scientific is dedicated to our customers needs. We offer a unique

environment that is driving technology and growth in our industry. bioo maintains a refreshing and respectful entrepreneurial environment that empowers our employees, collaborators and customers. We are creative thinkers and continue to add new products. We also promote collaborations to advance research and fuel new bioo scientific initiatives for the future.

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antibiotics / Veterinary DrugsMaxSignal™ antibiotics / Veterinary Drugs test Kits are designed for the quantitative analysis of veterinary drug residues such as chloramphenicol (cAP), crystal violet/LcV, diazepam, enrofloxacin, fluoroquinolone, furaltadone (AmoZ), furazolidone (AoZ), gentamicin, malachite green/LmG, streptomycin, sulfamethazine, tylosin and other drug residues in animal feed, crab, eggs, fish, honey, meat, milk, milk powder, serum, shrimp, urine and other matrices.

HormonesMaxSignal™ Hormone elIsa test Kits are designed for the quantitative analysis of beta-agonists such as bitolterol, carbuterol, cimaterol, clenbuterol, diethylstibestrol (Des), ractopamine, salbutamol, zilpaterol and other hormones in animal tissue, animal feed, urine and other matrices.

As one of the leading companies in the field, we develop, manufacture and market a wide range of food and feed safety test products covering Antibiotics/Veterinary Drugs, Hormones, microbiological tests, mycotoxins, Phycotoxins, and scombrotoxin (Histamine). A majority of these determinations can be done by either HPLc, Lc-ms or Gc-ms; however, these traditional methods usually require very expensive preparation procedures, longer processing time, experienced technicians or radioactive materials. bioo eLisA kits, on the other hand, provide a simple, rapid, sensitive and cost-effective screening method.

bioo MaxSignalTM eLisA test kits are developed and produced with bioo proprietary antibodies and reagents to achieve high sensitivity and low detection limits. our rapid, high recovery and cost-effective extraction methods are heavily tested using real samples from different regions of the world and constantly validated by world-wide reference labs. A bioo eLisA kit contains one 96-well plate (8 wells x 12 strips) and reagents, including standards and buffers, required to perform the test. it has the capacity for 96 determinations or testing of 42 samples in duplicate. the shelf life is 12 months when the kit is properly stored. Procedures for sample extraction are described in detail in the manual.

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M A X I M I Z E S C I E N C E F O R L I F E

B i O O F O O d & F E E d SA F E T y O V E R V i E W

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Microbiological tests bacteria exist in a range of environments but impose danger to health when found in food and feedstuffs. food products and their raw materials such as poultry meat, fish, shrimp, pork, and eggs can provide the perfect growth medium for microbes. for example, when salmonella contaminated food is ingested it may result in the disease known as salmonellosis. clinical symptoms include vomiting, diarrhea and nausea; this is referred to as gastroenteritis and in severe cases may result in death. the detection of bacteria in food samples by conventional microbiological techniques is often difficult, laborious and time-consuming. several days are required to culture the bacteria from a sample followed by a final isolation and identification by serological and biochemical techniques. bioo enzyme immunoassays (eLisA), however, offer a faster method of identification as compared with traditional techniques.

Mycotoxins mycotoxin is a toxin produced by fungi, which includes mushrooms, molds and yeasts. it is a secondary fungal metabolite that induces a variety of toxic and carcinogenic effects when food contaminated with the toxin is ingested. there are two major kinds of fungi producing mycotoxins. one is fusarium species, which generate mycotoxins directly on agricultural crops, such as Don, Zearalenone, t-2 toxin and fumonisin, predominantly reach the food chain through cereals and cereal products. the other important group of fungi (e.g. Aspergillus and Penicillium species) occur during inadequate storage areas. the corresponding mycotoxins, such as Aflatoxins and ochratoxin A, are found in incorrectly stored crop products or in food products directly. UsfDA and eU have set up a range of mycotoxins permitted in food and animal feed.

phycotoxinsPhycotoxins are marine biotoxins that accumulate in fish and shellfish from their diet algae. there are three different types of phycotoxins: PsP (paralytic shellfish poisoning), DsP (diarrhetic shellfish poison) and AsP (amnestic shellfish poison). the consumption of phycotoxin-contaminated food can lead to serious health problems that can be recognized by the following symptoms: damaged nervous system, damaged digestive system and memory loss. toxin- contaminated mussels can be found worldwide in the summer time. Phycotoxins-residues can be determined by either HPLc, Lc-ms or Gc-ms. However, bioo enzyme immunoassays (eLisA) have considerable advantages over conventional methods.

scombrotoxin (Histamine)certain bacteria produce the enzyme histidine decarboxylase during growth. this enzyme breaks down free histidine, a naturally occurring chemical that is present in large quantities in some fish (scombridae, such as tuna, kahawai, mackerel, bonito and butter-fly kingfish). this results in the formation of histamine and other amines. Histamine fish poisoning (HfP) is frequently encountered if dead fish remain in set nets during warm sea temperatures, the fish is improperly refrigerated or when refrigeration is delayed. the symptoms of HfP mimic those of an ige-mediated food allergy: nausea, vomiting, diarrhea, hives, itching, red rash (flushing), and hypotension. the MaxSignalTM Histamine enzymatic Assay is an efficient screening tool for the detection of histamine in seafood samples. (See page 5 for details.)

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cat. # analYte fOrMat saMple tYpe1 stanDarDs (ppb) lOD* (ppb)

1008 Ractopamine eLisA Animal feed, Kidney, Liver, meat, milk, Urine 0 / 0.5 / 1.0 / 5.0 / 10 / 25 0.25 - 2.5 1009 beta-agonist eLisA Animal feed, meat, tissue, Urine 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.025 - 0.25** 1010 Diazepam eLisA Animal feed, Kidney, Liver, meat, milk, serum, Urine 0 / 0.01 / 0.05 / 0.1 / 0.5 / 1.0 0.05 - 0.1 1011 sulfamethazine eLisA Animal feed, Honey, Kidney, meat, milk, milk Powder, 0 / 0.25 / 0.5 / 1.0 / 5.0 / 10 0.75 - 6.25 serum, Urine 1012 Diethylstibestrol (Des) eLisA Animal feed, fish, meat, Urine 0 / 0.15 / 0.5 / 1.5 / 4.5 / 9.0 0.075 - 0.15 1013 chloramphenicol (cAP) eLisA Animal feed, crab, eggs, fish, Honey, meat, milk, 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.025 - 0.25 milk Powder, serum, shrimp, Urine 1014 streptomycin eLisA Animal feed, Honey, Kidney, Liver, meat, milk, 0 / 0.5 / 1.0 / 2.5 / 5.0 / 10 5.0 - 25 milk Powder, serum, Urine 1015 furazolidone (AoZ) eLisA eggs, fish, Honey, meat, serum, shrimp, Urine, milk 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.1 - 0.2 1017 enrofloxacin eLisA Animal feed, fish, Kidney, Liver, meat, milk, shrimp, Urine 0 / 0.1 / 0.25 / 0.5 / 1.0 / 5.0 0.5 - 1.0 1018 malachite Green sPs2 Water sample 0 / 1.0 / 2.5 / 5.0 / 10 / 25 ppm 1.0 1019 malachite Green/LmG eLisA Animal feed, fish, shrimp, Water sample 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.05 - 0.2 1020 furaltadone (AmoZ) eLisA eggs, fish, Honey, meat, serum, shrimp, Urine, milk 0 / 0.05 / 0.2 / 0.8 / 3.2 / 9.6 0.1 - 0.2 1021 clenbuterol eLisA Animal feed, meat, tissue, Urine 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.025 - 0.25 1022 salbutamol eLisA Animal feed, meat, tissue, Urine, serum 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.025 - 0.25 1023 total Antibiotics (in milk) microbial growth milk Positive/negative controls 4.0*** 1024 fluoroquinolone eLisA Animal feed, fish, Kidney, Liver, meat, milk, shrimp, Urine 0 / 0.1 / 0.25 / 0.5 / 1.0 / 5.0 0.5 - 1.0 1025 cimaterol eLisA Animal feed, meat, tissue, Urine, serum 0 / 0.25 / 1.0 / 5.0 / 20 / 50 0.125 - 1.0 1026 tylosin eLisA Honey, milk, tissue, Urine 0 / 1.0 / 5.0 / 10 / 25 / 50 2.0 - 20 1027 Gentamicin eLisA milk, serum, tissue, Urine 0 / 0.25 / 0.75 / 1.5 / 3.0 / 15 2.5 - 6.25 1028 total Antibiotics (in Honey) microbial growth Honey Positive/negative controls 4.0*** 1029 crystal Violet/LcV eLisA Animal feed, fish, shrimp, Water sample 0 / 0.05 / 0.15 / 0.5 / 1.5 / 4.5 0.05 - 0.2 1030 total Aflatoxin eLisA feed, Dried meat/fish, nuts, spices 0 / 0.025 / 0.075 / 0.15 / 0.3 / 0.9 0.5 1031 Zilpaterol eLisA Animal feed, meat, tissue, Urine, serum 0 / 0.1 / 0.15 / 0.3 / 0.4 / 0.6 0.05 - 0.5 1032 Histamine enzymatic Assay fish/seafood, fish meal, milk, Wine 0 / 1.0 / 2.0 / 4.0 / 8.0 / 12 ppm 6.0 ppm 1033 sulfadiazine eLisA Animal feed, Honey, Kidney, meat, milk, 0 / 1.0 / 2.0 / 5.0 / 10 / 25 3.0 - 25 milk Powder, serum, Urine 1034 saxitoxin (PsP) eLisA fish/shrimp, mussels, Water 0 / 0.02 / 0.04 / 0.08 / 0.16 / 0.32 0.4 - 1.2 1035 Zearalenone eLisA feed, cereal, Dried meat/fish, milk, seed 0 / 0.1 / 0.25 / 0.5 / 1.5 / 4.5 1.0 1036 ochratoxin A eLisA feed, cereal, Dried meat/fish, milk, seed 0 / 0.15 / 0.45 / 1.35 / 4.05 / 12.15 1.5 1037 t2 eLisA feed, cereal, Dried meat/fish, milk, seed 0 / 1 / 5 / 10 / 25 / 50 10 1038 fumonisin eLisA feed, cereal, Dried meat/fish, milk, seed 0 / 5 / 10 / 25 / 50 / 100 50 1039 salmonella eLisA feed, fish, meat Positive/negative controls 10^5 cells/mL 1040 e. coli o157 eLisA feed, fish, meat, Vegetables Positive/negative controls 10^5 cells/mL

*Detection limits vary in regard to different sample types; see product insert for details ** Detection limit on clenbuterol; see product insert for detailed detection limits on other beta-agonists *** Detection limits on Penicillin and Ampicillin; see product insert for detailed detection limits on other antibiotics 1. Preparation protocols for samples other than above can be made available upon request 2. sPs: solid Phase separation

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B i O O F O O d & F E E d SA F E T y P R O d U C T S

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MaxSignal™ Malachite green/leucomalachite green (lMg) elIsa test Kit (patent-pending) catalOg #: 1019

this product is the first commercially successful eLisA kit for detecting malachite Green/Leucomalachite Green (LmG) in fish, shrimp, fish/shrimp feed or water samples of fish farms or fishponds. malachite Green (basic Green 4) has been widely used all over the world in the fish farming industry as a fungicide, ectoparasiticide and disinfectant. However, it has been shown that malachite Green is highly cytotoxic to mammalian cells and also acts as a liver tumor-enhancing agent. this has led to a prohibition of malachite Green for the treatment of fish and shrimp used for food production.

to prevent malachite Green residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that provide rapid, accurate, and reliable results at the required sensitivity. currently, malachite Green residues are determined by either HPLc or Lc-ms method with extremely expensive and time-consuming preparatory procedures. the MaxSignalTM malachite Green/LmG eLisA test Kit provides a simple, rapid, sensitive and cost- effective enzyme immunoassay (eLisA) screening method, which enables government agencies, seafood processors, as well as quality assurance organizations, to detect malachite Green to as low as 0.1 ng/g or ppb level in fish and shrimp matrices.

The unique features of the kit are:

1. High recovery (80 - 95%), and rapid extraction method for fish/shrimp or water samples.

2. chemical conversion of LmG to malachite Green before detection. chromatic malachite Green can be rapidly bio-converted into colorless LmG in fish (~80%). the special oxidant solution in the kit can convert LmG back to the malachite Green form which can be detected by the kit.

3. Providing a 96-well high throughput quantitative detection method. the detection limit is 0.1 ng/g (ppb) for fish/shrimp samples.

4. the processed samples can be directly used for liquid chromatography confirmation.

5. High reproducibility.

since its launch, this kit has generated enormous interest and positive reviews from both government reference labs and private sector labs. see sample reviews on page 13.

Also, see our similar product MaxSignal™ crystal Violet/ leucocrystal Violet (lcV) elIsa test Kit catalOg #: 1029

MaxSignal™ Histamine enzymatic assay Kit (USDA research grant supported*) catalOg #: 1032

the MaxSignal™ Histamine enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in seafood, meat, milk and wine. Unlike eLisA-based methods, this kit uses a rapid and direct enzymatic reaction to determine the amount of histamine in samples.

The unique features of the kit are:

1. Very rapid and direct procedure (complete procedure takes 35 minutes from start to finish - no wash steps needed).

2. convenient aqueous extraction method with high recovery. Harsh extractions using organic solvents not required.

3. High sensitivity and low detection limit (6 ppm).

4. A very rapid and robust enzyme-based assay which does not require chemical derivatization, sample filtration or expensive instrumentation.

5. Direct and simple assay procedure saves time and effort with minimal handling of samples; compatible with scale-up for high-through sample screening.

6. High reproducibility.

* UsDA sbiR # 2008-00294

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B i O O F O O d & F E E d SA F E T y F E AT U R E P R O d U C T S

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bioo scientific offers a wide range of products to assist with research and preclinical testing. We develop and produce a large assortment of in vivo RnAi products to help facilitate the progress of drug development. We also offer a full line of cytokine eLisAs as well as monoclonal and polyclonal antibodies that cover a wide range of research areas.

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M A X I M I Z E S C I E N C E F O R L I F E

B i O O R E S E A R C h & P R E C L i N i CA L T E S T i N G O V E R V i E W

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categOrY prODuct naMe catalOg # QuantItY*

MaxSuppressorTM In Vivo RNA-LANCEr 3407 1 Kit

3410-02 0.75 mL

MaxSuppressorTM Cell Culture RNA-LANCEr 3410-03 1 mL

3410-04 1.5 mL

Delivery Her2 Antibody conjugated to Protamine 3601 2 mg

MaxSuppressorTM Directed Delivery Agents cD33 Antibody conjugated to Protamine 3602 2 mg

VeGfR2 (KDR) Antibody conjugated to Protamine 3603 2 mg

cD4 V1 Domain Antibody conjugated to Protamine 3604 2 mg

MaxSuppressorTM Rnase Free Sterile Saline 3410-05 100 mL

MaxSuppressorTM Rnase Free Sterile Ringers 3410-06 100 mL

Detection MaxSuppressorTM Anti-siRNA Monoclonal Antibody 3450-01 100 µL

MaxSuppressorTM ß-galactosidase ELISA Kit 3420-01 1 Kit**

Reporter Assays MaxSuppressorTM Luciferase ELISA Kit 3420-02 1 Kit**

MaxSuppressorTM D-Luciferin 3420-03 60 mg

MaxSuppressorTM GAPDH ELISA Kit 3401 1 Kit**

MaxSuppressorTM Twinfilin 1 ELISA Kit 3430-02 1 Kit**

controls miR-1 miRnA molecule 3430-03 1 mg

MaxSuppressorTM In Vivo Synthetic miRNA Controls let-7a miRnA molecule 3430-04 1 mg

negative control miRnA molecule 3430-05 1 mg

MaxSuppressorTM Bradford Assay Kit 3440-01 1 Kit**

other MaxSuppressorTM In Cell ELISA Kit 3440-02 1 Kit***

MaxSuppressorTM Protein Extraction Buffer 3440-03 100 mL

*other quantities are available upon request.**one kit allows for 96 reactions.***one kit allows for 192 reactions.

B i O O R E S E A R C h & P R E C L i N i CA L T E S T i N G P R O d U C T S

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naMe cat. # assaY range (pg/ml) sensItIVItY (pg/ml)

mouse Gm-csf 2002 10 - 640 5

mouse Granzyme b 2014 50 - 3200 25

mouse ifn-y 2013 10 - 640 5

mouse iL-1α 2003 10 - 640 5

mouse iL-1ß 2004 8 - 512 4

mouse iL-2 2005 16 - 1024 8

mouse iL-4 2006 12 - 768 6

mouse iL-5 2007 16 - 1024 8

mouse iL-6 2008 10 - 640 5

mouse iL-12/iL-23 (total p40) 2015 10 - 640 5

mouse iL-12 p70 2009 40 - 2560 20

mouse iL-13 2010 25 - 1600 12.5

mouse iL-15 2011 20 - 1280 10

mouse iL-17 2012 16 - 1024 8

mouse iL-23 2016 30 - 1920 15

mouse mcP-1 (ccL2) 2017 20 - 1280 10

mouse tGf-ß1 2018 60 - 3840 30

mouse tnf-α 2001 15 - 960 7.5

Human Gm-csf 2102 16 - 1024 8

Human ifn-γ 2116 5 - 640 2.5

Human iL-1 2103 16 - 1024 8

Human iL-1α 2109 1.6 - 102.4 0.8

Human iL-1ß 2110 1.2 - 76.8 0.6

Human iL-2 2104 40 - 2560 20

Human iL-3 2117 20 - 1280 10

Human iL-4 2108 4 - 256 2

Human iL-5 2106 16 - 1024 8

naMe cat. # assaY range (pg/ml) sensItIVItY (pg/ml)

Human iL-6 2107 10 - 640 5

Human iL-7 2118 0.25 -16 0.125

Human iL-10 2113 1.25 - 80 0.625

Human iL-11 2119 30 - 1920 15

Human iL-12 2120 8 - 1024 4

Human iL-13 2121 30 - 1920 15

Human iL-15 2122 4 - 512 2

Human iL-16 2123 30 - 1920 15

Human iL-17 2124 30 - 1920 15

Human iL-20 2125 60 - 3840 30

Human iL-22 2126 10 - 640 5

Human iL-27p28 2127 8 - 512 4

Human iL-29 2128 30 - 1920 15

Human insulin 2111 100 - 6400 50

Human Leptin 2112 15 - 960 7.5

Human mcP-1 2101 16 - 1024 8

Human tGf-ß 2115 25 - 1600 12.5

Human tGf-ß1 2129 30 - 1920 15

Human tGf-ß2 2130 30 - 1920 15

Human tnf-α 2105 8 - 512 4

Human tnf-ß 2114 8 - 512 4

Rat Gm-csf 2201 16 - 1024 8

Rat ifn-γ 2204 16 - 1024 8

Rat iL-1ß 2205 30 - 1920 15

Rat iL-6 2202 16 - 1024 8

Rat iL-10 2206 60 - 3840 30

Rat tnf-α 2203 20 - 1280 10

B i O O Cy T O K i N E ASSAy S

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class suBclass naMe/antIgen clOne cat. # QuantItY* integrins integrin αL (cD11; LfA-1 α subunit) 38 3510-02 100 µg integrin αL, αm, αX/ß2 (cD11/ cD18) 24 3510-03 100 µg cell Adhesion Proteins intracellular Adhesion molecules (icAms) icAm-1 (cD54) 15.2 3510-04 100 µg icAm-3 (cD50) icAm 3.1 3510-05 100 µg neural Adhesion molecules neural cell Adhesion molecule (ncAm; cD56) UJ13A 3510-06 100 µg tRf1 tRf1 3H11 3511-01 100 µg cell cycle Proteins tRf2 mtA18 9f10/1 3511-02 100 µg cdc2 (cell Division control Protein 2; cDK1) 17 3511-04 100 µg cytochrome P450 cYP 1A2 D15 (16Vii f10f12) 3512-01 100 µg DnA Repair & Replication Proliferating cell nuclear Antigen (PcnA) Pc10 3524-01 100 µg extracellular matrix Proteins fibronectin fn-3 3521-01 100 µg chitinase Proteins YKL-40 3D5 3513-01 100 µg Growth factors iGf-bP (insulin-like Growth factor binding Protein) iGfbP2 3513-02 100 µg Vascular endothelial Growth factor (VeGf) Vascular endothial Growth factor (VeGf) VG-1 3513-03 100 µg cD1c (Human thymus Leukemia Antigen) m241 3514-02 100 µg cD3 (Purkinje cell antigen, t3) UcH-t1 3514-03 100 µg cD4 V1 domain Qs4120 3514-01 100 µg cD5 (Leu-1) UcH-t2 3514-04 100 µg cD5 (Leu-1) H65 3514-05 100 µg cD8 (Leu-2) 14 3514-06 100 µg cD8 (Leu-2) UcH-t4 3514-07 100 µg cD8 (Leu-2a) m236 3514-08 100 µg cD14 UcH-m1 3514-09 100 µg Leukocyte markers cD35 (Human complement receptor 1; cR1) e11 3514-10 100 µg cD36 (thrombospondin receptor) smϕ 3514-11 100 µg cD45 (Leukocyte common Antigen; LcA) c11 3514-12 100 µg cD45 (Leukocyte common Antigen; LcA) Hle-1 (2D1) 3514-13 100 µg cD45R 351c5 3514-14 100 µg cD45 Ro UcH-L1 3514-15 100 µg cD55 (Decay Accelerating factor:DAf) 67 3514-16 100 µg cD64 (fcg-Receptor i; fcg-Ri) 10.1 3514-17 100 µg cDw90 (thy-1) athy-1A1 3514-18 100 µg fibrin α chain Uc45 3514-19 100 µg eGfR (HeR1) eGfR1 3515-01 100 µg Receptors epidermal Growth factor Receptors (eGfR) c-erb b3 (HeR3) sGP1 3515-02 100 µg c-erbb4 (Her4) HfR1 3515-03 100 µg Alkaline Phosphatase (Human intestinal) AAP.1 3523-01 100 µg signal transduction Alkaline Phosphatases Placental alkaline phosphatase (PLAP) HD 8b6 3523-02 100 µg bcR-AbL 7c6 3523-03 100 µg signaling intermediates GAPDH 6c5 3516-01 100 µg

structural Proteins Keratins Keratin 8 LP3K 3522-01 100 µg

Keratin 19 bA16 3522-02 100 µg tissue specific Antigens mucin 1 (muc1) sm3 3517-01 100 µg c-Jun (phosphorylated) c-J 4c4/1 3518-01 100 µg c-myc 9e10 3518-02 100 µg n-myc nmYc-1 3518-03 100 µg transcriptional Regulators PARn 3518-04 100 µg RnA Polymerase ii (RnAP ii) Phosphorylated RnA polymerase ii (RnA pol ii) ctD 8A7 3518-05 100 µg RnA Polymerase ii (RnA pol ii) ctD 4H8 3518-06 100 µg

tumor suppressor Proteins p53 Do-1 3519-01 100 µg

Rb Rb1 (1f8) 3519-02 100 µg

Viral Proteins Human Papilloma Virus (HPV) Proteins HPV-18 e6 (early protein 6) bf7 3525-01 100 µg

simian Virus 40 (sV40) Proteins sV40 t Antigen PAb 416 3525-02 100 µg*other quantities are available upon request.

B i O O A N T i B O d i E S

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Add RnAi agent into antibody-protamine conjugate

RnAi agent binding to protamine

inject

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In Vivo rna-lancer catalOg #: 3407

MaxSuppressorTM In Vivo RNA-LANCEr uses a novel non-toxic blend of fat-soluble naturally-occurring hydrophobic molecules to efficiently deliver RnAi agents into mouse organs. the advanced formulation enables users to deliver RnAi agents, in vivo, for target validation and drug target identification purposes. After formulating RnAi agents with MaxSuppressorTM In Vivo RNA-LANCEr, the RnA becomes stable in serum, preventing the need for stability modifications which reduces the synthesis cost.

the preparation time takes less than one (1) hour and can be performed in any standard laboratory setting. simply mix the RnAi agent with the semi-dry lipid cake of MaxSuppressorTM In Vivo RNA-LANCEr, sonicate and inject as shown in the figure below.

Dried RnAi agent/RnA-LAncer cake is stable for extended periods of time at -20°c allowing preparation of the small RnAs to be done days before the injection is performed. injection of this agent via the tail vein has been shown to give the most efficient gene reduction in the liver, lung and kidney. However, the delivery route can be focused so that more efficient uptake and reduction may be obtained in your desired destination. the siRnA may be directly injected into a subcutaneous tumor or into a specific organ using surgical procedures.

Directed In Vivo Delivery agents catalOg # Her2 antibody conjugated to protamine 3601 cD33 antibody conjugated to protamine 3602 Vegfr2 (KDr) antibody conjugated to protamine 3603 cD4 V1 Domain antibody conjugated to protamine 3604

to assist in the directed delivery of siRnA molecules into animals, bioo offers antibodies conjugated to protamine sulfate. Protamine sulfate is a small, positively charged, arginine rich protein which is traditionally used to reverse the anticoagulant effects of heparin by binding to it. it has been found that protamine sulfate can also associate with nucleic acids such as siRnA and miRnA molecules. for this reason protamine sulfate conjugated antibodies have significant potential to be used with RnAi agents for the directed delivery of these agents into animal tissues via cell surface receptors.

to generate RnAi agent bound to protamine sulfate conjugated antibody, simply mix the RnAi agent with the conjugated antibody in the antibody storage buffer and incubate at 30°c for 15 minutes. following the incubation the RnA/antibody complex can be diluted into a sterile saline solution and injected directly into the animal.

Add RnAi agent into (In vivo) RnA-LAncer

Dried lipid cake (In vivo RnA-LAncer)

sonicate

inject

Lipid bilayer

encapsulated siRnA

R E S E A R C h & P R E C L i N i CA L F E AT U R E P R O d U C T S

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gapDH elIsa catalOg #: 3401

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis where it serves to break down glucose for energy and carbon molecules. GAPDH has also been found to play a role in membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RnA export, prostate cancer progression, programmed neuronal cell death, DnA replication, and DnA repair.

Particularly useful when studying RnAi induced gene silencing, the MaxSuppressorTM GAPDH ELISA kit can be used to detect GAPDH knockdown in tissues from animals that have been treated with a GAPDH RnAi agent. With this quantitative assay, users can rapidly analyze the impact of siRnA or shRnA on GAPDH levels in mouse, human or rat organs and body fluids including serum.

Human/Mouse twinfilin 1 elIsa catalOg # 3430-02

this kit enables users to detect twinfilin 1 protein (also known as protein tyrosine kinase 9, A6 protein tyrosine kinase, and PtK9 protein tyrosine kinase 9) in a quantitative fashion in human and mouse derived cells and tissues. this kit contains all of the components needed to extract protein from cells or tissues and to detect twinfilin 1 protein. this eLisA assay is particularly useful for researchers studying miRnA function in mammalian cells and animals as miR-1 muscle specific miRnA directly impacts twinfilin 1 mRnA at the posttranscriptional level. following the delivery of the miR-1 miRnA RnAi agent into cells or organs, the level of twinfilin 1 can be monitored to analyze the impact of delivery both in cell culture and in vivo. this enables users to rapidly analyze delivery of miRnA RnAi agents using an easy and cost effective eLisA assay. this method is more economical than performing PcR in standard conditions and allows for a more physiological impact of miRnA function a s compared with using PcR.

ß-galactosidase elIsa catalOg #3420-01

ß-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of ß-galactosides into monosaccharides. in E. coli the ß-galactosidase protein is encoded by the lacZ gene which is part of the lac operon. the ß-galactosidase protein is a large (120 kDa, >1000 amino acids) protein that forms a tetramer. the enzyme’s function in E. coli is to cleave the disaccharide lactose to form glucose and galactose so they can be used as carbon/energy sources. the lacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of gene promoters. ß-galactosidase is not typically expressed in mammalian cells, however; ß-galactosidase positive staining is a marker for mammalian cellular senescence. ß-galactosidase is a useful reporter gene both in cell culture and in vivo applications due to its ease of detection. this eLisA kit contains all the reagents required to quantitate the level of ß-galactosidase protein in cells and tissues. this kit can also be used to study the influence of drug treatments on ß-galactosidase staining in animal organs or cells in culture.

luciferase elIsa catalOg #3420-02

Luciferase is a generic name for enzymes, commonly found in nature, that produce light. the most commonly used Luciferase is produced by, Photinus pyralis, the firefly. Luciferase catalyzes a chemiluminescent oxidation–reduction reaction and although many kits are available to detect the enzymatic activity of this protein using light emission, these methods have several drawbacks. these assays have rapid decay kinetics; require the enzymatic activity of the Luciferase to be retained and they do not enable absolute quantification. in response to these issues, bioo has developed a highly sensitive eLisA assay which allows the researcher to completely measure the amount of firefly Luciferase in cell or tissue lysates. this eLisA Kit provides all of the components needed for rapid, and precise, quantification of firefly, Photinus pyralis, Luciferase in cultured mammalian cells or tissues.

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BIOO scIentIfIc Offers serVIces In tHree MaIn areas:

1. PRecLinicAL testinG seRVices: our Preclinical testing services provide a cost effective way of testing specific drug molecules, including RnA based drugs, in the small animal model system most appropriate for your specific goals. this service enables the researcher to take potential drugs against clinically important targets into a preclinical setting for further validation in an efficient fashion.

2. IN VIVo RnAi AGent seRVices: bioo offers custom RnAi services in the area of the whole organism. these experiments are typically not easy for most researchers to do as the techniques are very specialized and time consuming to initiate. We offer a cost effective way of testing specific siRnA molecules in the model system most appropriate for your goals. this enables researchers the ability to take siRnAs against clinically important targets directly into an animal environment for further validation in an efficient fashion with minimal down time.

3. AntiboDY seRVices: We offer both monoclonal and polyclonal antibody production services. bioo has developed a unique 5-week monoclonal Antibody Production service (patent-pending) to produce custom monoclonal antibodies against peptides and proteins. We understand the importance of antibody availability for research needs and therapeutic development. the benefit of bioo’s high volume throughput production allows end-users to access a greater number of antibody hybridomas for significantly less cost. We provide full antibody services for our customers, from antigen design to affinity purification and ascite fluid production. Using our exclusive method we can generate an effective antibody where other systems fail.

BiOO SERViCES

We have state of the art facilities where we perform these services and research expertise that is aggressively advancing the technology of in vivo RnAi activity, efficacy and delivery and monoclonal antibody manufacturing. our continued efforts in these areas will keep our service offering on the cutting edge of science. bioo services provide customers quality results with minimal start up and overhead costs. bioo scientific has a >2000 square foot, UsDA and oLAW approved, Animal facility. this facility is fully functioning to test drug compounds and RnAi

agents in numerous animal model systems. We follow all guidelines as specified by oLAW and “PHs Policy” and take tremendous care to provide a comfortable and safe environment for all animals.

bioo services provide a means of outsourcing preclinical testing, in vivo RnAi agent experiments and monoclonal and polyclonal antibody-manufacturing to highly trained professionals. We can help researchers achieve their objectives at a fraction of the cost and with minimal down time.

M A X I M I Z E S C I E N C E F O R L I F E

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cOntacttel: 1.888.208.2246 512.707.8993fax: 512.707.8122email: info@biooscientific.com

sales & custOMer suppOrtus: 1.888.208.2246 512.707.8952

Outside us: contact your local distributors or distribution@biooscientific.com

email: sales@biooscientific.com support@biooscientific.com antibodyservice@biooscientific.com foodfeedsafety@biooscientific.com RnAi@biooscientific.com

For up-to-date information on Bioo Scientific Corp’s complete range of products and services, go to www.biooscientific.com.

C U S T O m E R R E V i E W S & C O N TAC T i N F O R m AT i O N

M A X I M I Z E S C I E N C E F O R L I F E

re: Bioo scientific corporation’s MaxSignalTM Malachite green KitsThe recent FDA recall of potentially contaminated seafood products created quite a concern among several of our valued clients. our job at Q Laboratories was to alleviate these concerns by providing rapid and accurate malachite green results. The Bioo Scientific MaxSignalTM kits allowed us to meet the needs of our clients and the subsequent governing bodies in a timely manner. The new changes in the test kit standards are even further proof that Bioo Scientific works hard to improve the quality of their products and we will continue to turn to them for our test kit needs in the future.

erin s. crowley microbiology R&D supervisor Q Laboratories, inc. cincinnati, ohio

re: Bioo scientific corporation’s MaxSignalTM Malachite green KitsGorton’s relies on Bioo Scientific’s MaxSignalTM Malachite Green Kits to provide our laboratory with a rapid, sensitive and cost effective procedure to screen our products. The availability of the kit allowed Gorton’s to react quickly to current food safety and regulatory issues surrounding food contaminants. We are pleased with the accuracy and continuous improvements to the procedure developed by Bioo Scientific, which has resulted in a greater ease of use by laboratory technicians.

Allison Williamson QA Manager Gorton’s

Bioo Scientific Corp.3913 Todd Lane, Ste. 312Austin, TX 78744 USA Made in USA

1.888.208.2246www.biooscientific.come: info@biooscientific.com

Bioo scientific corp.3913 todd Lane, ste. 312Austin, tX 78744 UsA

M A X I M I Z E S C I E N C E F O R L I F E

1.888.208.2246e: info@biooscientific.comwww.biooscientific.com © 2008 bioo scientific corp.