Letters

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Abstracts / Toxicology

is of Alzheimer’s disease (AD), which is characterizedy progressive decline of cognitive function, formationf A beta plaques, and neurofibrillary trangles, and lossf neurons.

In the present study we have examined that treatmenty vitamin E + Statin in PDAPP aged mice comparedith control groups is able to decrease 20% in ABlaques levels of cerebral amyloidosis in necortex. More-ver, our results suggest that treatment by vitamins isble to prevent the disruption of basal cholinergic fore-rain system and prevent of loss of cholinergic basalorebrain neurons (Ms + HDB, VDB).

oi:10.1016/j.toxlet.2007.05.477

29aternal diets and early environmental exposure

nfluences weight maintenance and development ofhe offspring

ndre Wolterbeek, Simone Eussen, Renate Jansink,inda van der Horst, Marlies Otto, Ine Waalkens, Anjaijkstra, Florence Salmon, Heleen Wortelboer,ilrike Pasman, Henk Hendriks, Ivana Bobeldijk,idima de Groot

TNO Quality of Life, Zeist, Netherlands

besity and related diseases are major threats to healthn western societies.

To study (1) the impact of maternal diets on bodyeight of the offspring and (2) the effects of maternaliets in combination with environmental exposure onhe (neuro)development of the offspring, three groupsf female Wistar rats (n = 28–32) were kept on a con-rol diet, a western style type diet or a deficient dieturing 6 weeks premating, mating, gestation and lac-ation. On postnatal day 1 (PN 1) the F1-pups wereross-fostered to dams of the same group or to damsf the other two dietary groups to obtain nine differentroups. F1-pups were fed the various diets up to PN0 and body weight/mass and food intake were mea-ured regularly. To investigate environmental influences,he vulnerability for chemically induced neurotoxicity1-pups were treated subcutaneously with MAM (fromN day 2 to 5, twice daily) or MeHg (from PN 2

o 21).The results showed that: (1) Food consumption,

nergy intake and body weights of the dams differed

ignificantly between the groups. (2) Birth weight of theups of dams on a deficient diet were slightly, not signif-cantly, decreased. (3) Dietary habits during prematingnd gestation phases appeared to influence the growth

172S (2007) S1–S240 S189

of the pups during lactation, showing ‘catch-up’ growthof the deficient pups fostered to dams on the westernstyle type diet and, vice-versa, decreased growth of thewestern style type diet pups fostered to dams on a defi-cient diet. Dietary induced effects on body weights ofF1 animals were exacerbated by exposure to MAM orMeHg.

It was concluded that maternal diet manipulation had(transitory) effects on the offspring which, in turn, maybe responsible for increased vulnerability of the off-spring for (subtle) environmental influences at or shortlyafter birth causing life-long developmental effects.

doi:10.1016/j.toxlet.2007.05.478

P11 Food Safety

F01Ochratoxin A-induced renal gene expressionchanges in F344 rats after repeated oraladministration

Leire Arbillaga, Ariane Vettorazzi, Ana G. Gil, AdelaLopez de Cerain

Department of Food Sciences, Physiology andToxicology, Faculty of Pharmacy, University ofNavarra, Pamplona, Navarra, Spain

Ochratoxin A (OTA) is a nephrotoxic mycotoxin oftenfound in agricultural products. There is unequivocal evi-dence of renal carcinogenicity in male rats although itsmechanism of action is unknown. Direct genotoxicityversus epigenetic mechanisms are still under debate. Themost recent data based on biochemical and toxicoge-nomic evidence, indicate that chronic administration ofa carcinogenic dose of OTA results in a significant reduc-tion of renal antioxidant defence and increased oxidativeDNA damage.

This study has been designed to analyze renal geneexpression changes in F344 rats after repeated oral car-cinogenic dose of OTA dissolved in 0.1 M NaHCO3 pH7.4. For this purpose, 0.5 mg/kg bw OTA was adminis-tered daily by gavage to two groups (n = 5) of male F344rats and gene expression modulation was studied at 7 and21 days. Two control vehicles of five animals were alsoincluded in order to compare the gene expression pat-tern of each treated group with its corresponding control.Affymetrix Rat Genome 230 2.0 Gene Chips containing28.000 well-substantiated rat genes, were used. Modu-

lated genes were identified using BRB-ArrayTools andclassified in biological pathways using GenMapp.

Histopathological and biochemical data showedslight signs of nephrotoxicity. These findings will be