Materials Core Instrumentation:

1. UV-Vis-NIR spectrophotometer DU 640B (Beckman)

2. FTIR spectrophotometer FTIR-480 (Jasco)

3. Spectropolarimeter J-810 (Jasco)

4. Refractive index analysis instrument AR700 (Reichert)

5. CAM 200 optical contact-angle goniometer (KSV Instruments)

6. Ellipsometer GES 5, variable-angle spectroscopic (Sopra Inc)

7. Flame atomic absorption - AAnalyst 200 (Perkin Elmer)

8. SPR spectrometer Biacore X

9. MTS Synergie 100

10. HPLC/GPC system (Waters)

11. Plasma surface treatment unit (Harrick)

More details on each instrument are on the following slides.

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For more info, please contact Dr. Gulya Korneva: gkornev@clemson.edu

Spectrophotometer DU 640B, Beckman

Applications:Measures Absorbance and Transmission: 1) At fixed wavelength;2) Wavelength scan;3) Kinetics/timeCan be used in qualitative and

quantitative analyses.

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Wavelength range 190-1100 nm

Fourier Transform Infrared Spectrophotometer FTIR-480, Jasco

Applications:• structure analysis;• purity examinations;• quantitative analysis;• identification of unknown compounds:

liquids, semi-liquids and flexible solids;• biological materials study;with HATR plates ZnSe highly absorbing substances can be analyzed (polymers, rubber, fibers, etc);Specifications: Wavelength range: 360 to 7800 cm-1;• Resolution range: 0.5;0.9;2;4;8;16 cm-1

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Accessories: 1) MIRacleTM single reflection HATR (horisontal ATR, Pike technologies) with 2 mm round crystal ZnSe. 2) &3) HATR plates in “through” and “flat” configurations. “Through” – for liquid, powder, or paste, good for volatile compounds; “flat” – for the analysis of solid materials including polymer and film samples.

For more info: www.jascoinc.com/Libraries/Application_Notes/IR_01_03.sflb.ashx

Spectropolarimeter J-810, Jasco

SpecificationsLight source: 150W Xe arc lamp (N2 cooled)Wavelength range: 163 ~ 900 nmAccessories: Peltier type temperature controller Jasco PFD-425S Automated pH titrator Jasco ATS 429S

Measures the circular dichroism (CD) and optical rotary dispersion (ORD) of the sample. Applications: • Allows to verify the chirality of

molecules or compounds;• to estimate fractions of each

different conformation in protein structure;

• the conformational changes of molecules with temperature/and pH can be studied.

Useful for verifying that the protein is in its native conformation. • Protein folding studies. • Purity testing of optically active

substances.4

Refractive index analysis instrument – Reichert AR700

For more info: http://www.reichertai.com/products.html?productID=17

Temperature Controlled Automatic Refractometer

Applications:Measures refractive index of transparent, translucent, and opaque liquids.

Features:Reading range of 1.33000 to 1.60000 Refractive Index and the full Brix scale.Precision: to 0.01 Brix or 0.00001

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CAM 200 (KSV)

CAM 200 is a video camera based fully computer controlled contact angle meter.

Applications:• Contact angle

measurements;• Surface free energy

measurements;

For more info: http://www.terralab.biz/edoc/ksv/KSV_Cam_200_Contact_Angle_Surface_Tension_Meter.pdf

CCD Camera

• Absorption and wetting;• Surface/interfacial tension

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Variable-angle spectroscopic ellipsometer GES 5 (Sopra Inc)

Spectroscopic ellipsometry (SE) is a non-contact, non-destructive optical technique.Applications: • measures material refractive

indices; • layer thicknesses of thin film

layers on flat substrates; • up to 7 layers can be analyzed

in terms of thickness and optical characteristics for each layer. Features:

Thickness measured: from monoatomic to several microns thick. The beam size: 1 mm2 and 10 mm2; The variable angles: from 7º to 90º with variable +/- 0.01º;spectral range from 200 nm to 800 nm

7 For more info: http://www.sopra-sa.com/ges5e-for-retd-pdt-5-16.php

Flame atomic absorption spectrometer - AAnalyst 200, Perkin Elmer

Applications: • for determining very precisely the

concentration of a particular element in a liquid sample. Can detect from 1 to 1000 µg/L.

For each element, the specific lamp is required. Currently, Ca lamp is available.The list of lamps/elements for analysis on AAnalyst 200 can be found on https://www.vwrcanlab.com/programs/equipment/pdf/Model%20AAnalyst%20200%20Spectrometers.pdf

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Biacore is a methodology for measuring the biomolecular interactions in real time using the phenomenon of surface Plasmon resonance (SPR).Applications: it allows to study: • Kinetics of binding• Solution affinity• Steady state affinity• Yes/No binding• Ligand fishing• Affinity ranking• Protein concentration assays• Protein percent activity assays• ThermodynamicsInteractions between nucleic acids, lipids, small molecules, whole cells, and surfacescan be studied.

BiAcoreX SPR spectrometer

Specifications:Two Flow cell capabilityTemperature regulation = 4-40oCFlow rates 1-100μL/min, steps of 1 μLRequired sample volume: injection volume

(min-5 μL +20 μl)Molecular weight detection: >180 DaRefractive index range: 1.33 - 1.40 Sensitivity ≈ 20 RU, where RU, resonance

units, 1RU = 1 pg/mm2 of sensor surface.

9Some applications are described in a paper by Y. Wei and R. A. Latour, Langmuir 2009, 25(10), 5637–5646

For more info: http://web.bf.uni-lj.si/bi/sprcenter/BiacoreX.pdf

Materials Testing System (MTS) Synergie 100

Testing: Tensile, Flex, Peel/Tear, Strain, and Compression test methods.1) Loadings available: 10 N (2 lbf)2) Speed range: 0.001-1000 mm/min (0.00004-39 in/min)3) Max specimen thickness for grips: 13 mm (0.5 in) 4) 50 mm (2.0 inch) diameter lightweight compression platensFace set, 25x25 mm rubber, for 100/2000 advantage pneumatic and screw action grips.

For more info: http://www.mts.com/en/Bio/Materials/index.asp

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HPLC/GPC system (Waters)

Applications: high resolutionseparations and purificationsIncludes: I. Binary Gradient Pumping

System (1525)II. Autosampler (2707)III. Photodiode Array Detector (2998)IV. Refractive Index Detector (2414)V. Column HeaterVI. Fraction Collector

Specifications: VII. Binary Gradient Pumping System: Flow Rate Range: 0 to 10 ml/min; max

operating pressure: 6000 psi.VIII.Autosampler: Loop Volumes: 100 and 500 µl.IX. Refractive Index Detector: Refractive index range: 1.00 to 1.75 RIU.X. Column Heater: Temperature range: 20oC to 60oC; column capacity: up to

four 7.8 mm x 300 mm columns

For more info: http://www.waters.com/waters/nav.htm?locale=en_US&cid=51425611

Plasma cleaner/sterilizer, Harrick

For more info: http://harrickplasma.com/applications_biomaterials.php

Applications: • Plasma activation of substrate surfaces to

render surface hydrophilic; promote attachment and adhesion of functional biological species or coatings to:

1) Enhance cell adhesion, coverage, and proliferation on tissue scaffolds.

2) Promote adsorption of selective functional biological species while resisting, adhesion of bacteria and fouling microorganisms. • Removes organic contamination from surfaces.

• Modifies or enhances the physical and chemical characteristics of surfaces: The high energy plasma particles combine with the contaminant to form carbon dioxide (CO2) or methane (CH4).

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