magnetically-guided nanoparticles for targeted drug delivery
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Magnetically-Guided Nanoparticles for Targeted Drug Delivery. Presentation for RET program July 7, 2011 Seth Baker Advisors: Dr . Andreas Linninger , Eric Lueshen , Madhawa Hettiarachchi Laboratory for Product and Process Design University of Illinois- Chicago - PowerPoint PPT PresentationTRANSCRIPT
LPPD University of Illinois Chicago, LPPP. Summer 2008
Magnetically-Guided Nanoparticles for Targeted Drug Delivery
Presentation for RET program July 7, 2011
Seth BakerAdvisors:
Dr. Andreas Linninger, Eric Lueshen, Madhawa Hettiarachchi
Laboratory for Product and Process Design University of Illinois- Chicago
Department of Chemical Engineering
University of Illinois Chicago, LPPD, Summer 2011
LPPD University of Illinois Chicago, LPPP. Summer 2008
Project Summary
• Neurological therapeutics have limited success due to natural barriers such as the blood brain barrier. Methods to circumvent or penetrate the blood brain barrier include:
Receptor- Mediated Transport Molecular Trojan Horses
Intranasal Osmotic DisruptionConvection-Enhanced Delivery Macrophage Transport
• Over 7 million Americans suffer from neurological conditions such as Alzheimer’s, Parkinson’s, Brain Cancer, and Stroke. These conditions have direct and indirect costs of over $200 billion dollars annually in the United States.
Magnetically – guided nanoparticles could offer targeted detection,
diagnosis and treatment options for these neurological conditions.
University of Illinois Chicago, LPPD, Summer 2011
LPPD University of Illinois Chicago, LPPP. Summer 2008
Benefits of Magnetic Nanoparticles
• Nanoparticles can be coated or loaded with various therapeutics.
University of Illinois Chicago, LPPD, Summer 2011
• Allows for more targeted drug delivery resulting in lower dosage and systemic toxicity. Particles are biocompatible and biodegradable.
LPPD University of Illinois Chicago, LPPP. Summer 2008
Determine Magnetic Quality
University of Illinois Chicago, LPPD, Summer 2011
Time = 0 sec Time = 60 sec Time = 120 sec Time = 180 sec
Time = 240 sec Time = 300 sec Time = 360 sec Time = 420 sec
Magnetite nanoparticles were guided toward a 173 lb pull force magnet over an 8
minute experiment.
LPPD University of Illinois Chicago, LPPP. Summer 2008
Agarose Gel Experimental Design
University of Illinois Chicago, LPPD, Summer 2011
0.5 % Agarose gel
173 lb pull force magnet
Syringe loaded with magnetite nanoparticles
?
Control
Petri dish
LPPD University of Illinois Chicago, LPPP. Summer 2008
Agarose Gel Early Results
University of Illinois Chicago, LPPD, Summer 2011
Control 35 lb pull force 173 lb pull force
Base of petri dish Syringe line
Magnetic nanoparticles were guided toward the magnets through 0.5% agarose gel
LPPD University of Illinois Chicago, LPPP. Summer 2008
Rat Brain Experiments
University of Illinois Chicago, LPPD, Summer 2011
Determining permeability of Prussian blue stain on unfixed rat brain
Rat brain in saline solution Rat brain in Prussian blue stain Coronal cross section with no staining
Set up for magnetic nanoparticle movement on unfixed rat brain
Coronal cross section of rat brain
Adding 10 µ l magnetite nanoparticles
173 lb pull force magnet and a control
LPPD University of Illinois Chicago, LPPP. Summer 2008
Future Work
• Continue with magnetic nanoparticle experiments to reduce agglomeration of nanoparticles and allow for convection enhanced delivery.
• Determine the most effective protocol for administrating and measuring magnetically-guided nanoparticles in rat brain samples.
• Investigate methods of manufacturing nanoparticles for better visualization in angiogram – possible gold plated magnetite.
University of Illinois Chicago, LPPD, Summer 2011
LPPD University of Illinois Chicago, LPPP. Summer 2008
Acknowledgements
• NSF EEC-0502272 Grant, Chicago Science Teacher Research
• Dr. Andreas Linninger
• Members of the LPPD – Eric Lueshen, Sukhi Basati, Joe Kanikunnel
University of Illinois Chicago, LPPD, Summer 2011