maglisto 5m cell total rna extraction kit - bioneer.co.kr magnetic nano beads enable the rapid...

K-3610 K-3611

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User’s Guide

MagListo™ 5M Cell Total RNA Extraction Kit



Kit for the extraction of total RNA from wide range of cell types using MagListo™

User’s Guide

K-3610 K-3611

Version No.: 3.0 (2017-03)

Please read all the information in booklet before using the unit

Bioneer Corporation

8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon

34302, Republic of Korea

Tel: +82-42-930-8777

Fax: +82-42-930-8688

Email: [email protected]

www.bioneer.com

MagListo™ is a trademark of Bioneer Corporation.

Copyright 2017. Bioneer Corporation. All Rights Reserved.

mailto:[email protected]


Contents

I. Overview 1

II. Kit Components 1

III. Storage 2

IV. Intended Use 2

V. Safety Warnings and Precautions 2

VI. Warranty and Liability 2

VII. Technical Assistance 3

VIII. Quality Management 3

IX. Kit Specifications 4

Extraction of total RNA from small amount of sample 4

X. Sample preparation 5

XI. Principle 5

XII. Magnetic NanoBead Information 6

XIII. Guidelines for MagListo™ Magnetic Separation Rack 7

XIV. Materials and Equipment Needed But Not Provided 7

Types of the Magnetic Separation Rack 8

XV. Procedure 9

XVI. Protocols 10

Before you begin 10

A. RNA Extraction from Cultured Cells 10

B. RNA Clean up (RNA Purification) 15

C. ONE Step RNA Cleanup (DNase Treatment) 16

XVII. Appendix 18


Troubleshooting guide 18

Experimental data 20

XVIII. Ordering Information 22

XIX. Explanation of Symbols 23

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I. Overview

Description

MagListo™ 5M Cell Total RNA Extraction Kit utilizes Magnetic Nano Beads to extract total RNA from

cultured cells, with the aid of the MagListo™ Magnetic Separation Rack. The use of MagListo™

Magnetic Separation Rack along with this kit greatly increases user convenience by reducing the total

RNA extraction time without centrifugation.

Features and Benefits

- Magnetic Nano Beads enable the rapid nucleic acid extraction

- No requirement of expensive instruments

- A single kit serves mini or midi scale experiment

Applications

Applicable to assays requiring RNA, including, but not limited, RT-PCR, cDNA synthesis, Northern,

dot, and slot blot analyses, Primer extension, Poly A+ RNA selection, Microarrays

II. Kit Components

MagListo™ 5M Cell Total RNA Extraction Kit *K-3611

Buffer ① (Binding) 25 ml x 2 ea

Buffer ② (1st Washing) 80 ml x 1 ea

Buffer ③ (2nd Washing) 80 ml x 1 ea

Buffer ④ (3rd Washing) 120 ml x 1 ea

Buffer ⑤ (Elution) 25 ml x 1 ea

Magnetic Nano Bead - RNA 1.8 ml x 6 ea

*mini – 100 rxn, midi – 20 rxn

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III. Storage

MagListo™ 5M Cell Total RNA Extraction Kit should be stored dry at room temperature. It can be

stored for up to 2 years if it remains sealed.

IV. Intended Use

MagListo™ 5M Cell Total RNA Extraction Kit is intended for research use only. This kit is not intended for

human or veterinary diagnostics.

V. Safety Warnings and Precautions

Please inquire BIONEER’s Customer Service Center to obtain a copy of the Material Safety Data Sheet

(MSDS) for this product.

Before, during and after using this kit, all potentially hazardous materials (i.e. materials that may have

come in contacted with genetically recombinant samples) including tubes, tips and other kit contents

should be processed and discarded in accordance with applicable and appropriate regulations of the

municipality/government in which this product is being used. A user must also be equipped with basic

experimental techniques required for correct execution of the extraction experiments described in this

User’s Guide.

Some inventions applied in this kit may infringe existing patents in certain countries. The purchase of

this kit does not include or provide a license to perform such patented inventions. Users may be

required to obtain a license depending on the patent law of the country where this product is being

used. We do not condone nor recommend the unlicensed use of patented inventions.

VI. Warranty and Liability

All BIONEER products are manufactured and tested under strict quality control protocols. BIONEER

guarantees the quality of all directly manufactured products during the warranty period of one (1) year

from the date of purchase. If you find any issues regarding the product quality, please immediately

contact BIONEER’s Customer Service Center ([email protected]).

BIONEER does not assume any liability for the misuse of the product, i.e. using the product for any

purposes other than its intended purpose as described in the User’s Guide. BIONEER will only assume

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liability under the condition when the users disclose all related information regarding the issue to

BIONEER in written form within 30 days after occurrence of the issue.

VII. Technical Assistance

At Bioneer, we pride ourselves on being responsive to your needs. Our Technical Service Departments

are staffed by experienced scientists with extensive practical and theoretical expertise in Molecular

Biology and the use of Bioneer products. If you have any questions or would like to find out more

information about MagListo™ products, please contact us. We look forward to hearing from you!

Technical Support

For all technical questions and troubleshooting on Bioneer products and applications

Tel: +82-42-930-8777

Email: [email protected]

- In North America

Tel: +1-877-264-4300

Email:[email protected]

VIII. Quality Management

Every aspect of our quality management system from product development to supplier qualification

ensures that our products meet the world-class standards. Each lot of MagListo™ 5M Cell Total RNA

Extraction Kit is carefully tested by the quality control team.

mailto:[email protected]

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IX. Kit Specifications

Micro scale Mini scale Midi scale

Starting Cell Number 10 - 104 105 - 5 x 106 5 x 106 - 2 x 107

Extraction time < 8 min < 8 min < 13 min

Elution volume 30 - 100 µl 80 - 100 µl 100 - 500 µl

Expected RNA yield Up to 1 µg Up to 100 µg Up to 400 µg

Expected purity A260/280 > 1.9

*RNA content can vary greatly among cell types.

Typical RNA yield from cultured cell purified with MagListo™ 5M Cell Total RNA Extraction Kit.

Cell type (1x 106) Yield

Hela 10 - 15 µg

293T 20 - 30 µg

Balb/c 3T3 20 - 30 µg

Huh7 10 - 15 µg

Extraction of total RNA from small amount of sample

MagListo™ 5M Cell Total RNA Extraction Kit is also able to extract total RNA from a small quantity

of sample. Refer to “Cell Total RNA Extraction from Cultured Cell for Micro” in page 10 for more

details about RNA extraction from samples with a low number of cells (< 1 x 104).

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Table1. Growth area and Average cell yield in various culture dishes.

Cell culture dishes Growth area (cm2) Average cell yield

Multi well plate

6 well 9.6 1.2 x 106

12 well 4 4 x 105

24 well 2 2 x 105

48 well 1 1 x 105

96 well 0.35-0.6 4 x 104

Dishes

35 mm 8 1.2 x 106

60 mm 21 3 x 106

100 mm 55 8 x 106

150 mm 148 2 x 107

Flasks

50 ml 25 2.5 x 106

300 ml 75 1 x 107

X. Sample Preparation

Cultured cells can easily be harvested using a centrifuge. However, it might be difficult to extract total

RNA if cultured cells are too clustered. In this case, trypsin can be used to detach clustered cells. For

extraction, the number of cells should be less than 1 x 107, which is calculated with a cell counter. It is

recommended to keep samples on ice before use.

Xl. Principle

The MagListo™ 5M Cell Total RNA Extraction Kit is designed for the extraction of high purified total

RNA from cultured cells. The overall principle is based on adsorption of RNA onto the Magnetic Nano

Bead by chaotropic salt. For example, chaotropic agents in Buffer ① (Binding) contains guanidine

hydrochloride and guanidine thiocyanate, as which remove water molecules around RNA and silica

coated magnetic beads surface resulting in RNA then being captured by magnetic beads. The

Magnetic Nano Beads and RNA complexes are pulled and fixed on the tube wall using a magnetic

force, followed by washing with ethanol to remove debris and excessive salts. Finally, the captured

RNAs are then eluted by Buffer ⑤ (Elution), an aqueous solution with optimal pH.

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Lysis Binding Washing Elution

XII. Magnetic Nano Bead Information

Description

Magnetic Nano Beads have been developed to overcome shortcomings of existing resin and to automate

purification process. The principle of extraction using the Magnetic Nano Beads is that coated functional

group on the surface of the Magnetic Nano Beads bind with DNA and the Magnetic Nano Beads are then

isolated using external magnetic field.

Features

Fast binding guarantees higher throughput automation

Large Surface Area enables more sensitive assay

Globular structure increases specificity by decreasing non-specific binding

Specification

AccuNanoBead™ Silica Magnetic Nano Beads

Matrix Silica-coated Fe3O4

Average size 400nm

Ligand -OH

Working Temp. 0-100℃

Storage Store at room temperature upon receipt

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XIII. Guidelines for MagListo™ Magnetic Separation Rack

Description

MagListo™ Magnetic Separation Rack is designed for a fast, easy separation of the Magnetic Nano

Beads. These racks of different sizes allow users to choose the product according to their needs.

The following are recommended when handling the MagListo™ Magnetic Separation Rack

The product is made of acryl and plastic. Be careful not to drop the product as the dropping may

break the product.

When moving the product, take extra care not to drop the product as it may cause injury.

If the product is broken, do not discard it with bare hands as the sharp edges may cause injury.

When an extracted or purified nucleic acid is spilled on the product, immediately rinse it with

running water and clean it with 70% ethanol.

Acetone, Toluene, or other organic solvent may damage the acrylic and plastic part of the product,

which may lead to malfunction of the product. Rinse the product immediately when spillage of any

above mentioned solvents occurs as the expected DNA yield may not be obtained if the product is

damaged.

Make sure that a corrosive liquid does not spill on the magnet plate part of the product. If spillage

occurs, immediately rinse it off with running water as it may corrode the magnet during storage

and may degrade its performance.

XIV. Materials and Equipment Needed But Not Provided

1. Table-top microcentrifuge, 16,000 x g (>13,000 rpm) (mini scale)

2. Centrifuge with rotor capable of 3,000 x g (midi)

3. 1.5 ml or 2 ml tube (micro / mini scale)

4. 15 ml tube (midi scale)

5. Vortex mixer

6. Absolute ethanol

7. Thermal block

8. MagListo™ Magnetic Separation Rack

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Types of the Magnetic Separation Rack

Tube MagListo™ Magnetic Separation Rack Cat.No

1 ml tube with 8-cap strip MagListo™-8Ch Magnetic Separation Rack TM-1000

1.5 ml or 2 ml microcentrifuge tube MagListo™-2 Magnetic Separation Rack TM-1010

15 ml tube MagListo™-15 Magnetic Separation Rack TM-1020

(Note) Please refer to the ordering information in this User’s Guide for more information regarding

catalog number of racks designed for specific size of tubes.

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XV. Procedure-Cell Total RNA Extraction

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XVI. Protocols

Before you begin

1. Buffer ① (Binding) and Buffer ② (1st Washing) contains chaotropic salt. You should take the

appropriate laboratory safety precautions and wear gloves and lab goggle when handling.

2. The relative centrifugal force (RCF) is calculated in g as follows:

RCF = 1.12 x r x (rpm/1,000)2

Where ‘r’ is the radius of a rotor in cm, and ‘rpm’ is the speed of the rotor in revolutions per minute.

3. To inhibit RNase activity, we recommend adding β-mercaptoethanol to Buffer ① (Binding) before

use. Add 10 µl β-mercaptoehanol(>99%) per 1 ml Buffer ① (Binding).

A. RNA Extraction from cultured cell for Micro/Mini/Midi scale

1. (Harvest cell) Cells grown in suspension :

Count the cell number and centrifuge given number of cells (~1x104 (micro) / ~5x106 (mini) /

~2x107 (midi)) at 300 x g for 5 min.

Discard supernatant carefully and go to lysis & homogenization (go to step 3).

2. Cells grown in a monolayer: There are 2 different ways to collect cells grown in a monolayer.

a. Direct cell lysis on the culture dish:

Completely remove Cell Culture Medium and go to lysis & homogenization (go to step 3).

(Remaining medium may inhibit the RNA extraction)

b. Harvesting cells with trypsin:

Remove Cell Culture Medium and wash the monolayer with DPBS. Add 0.1%-0.25% typsin to

the washed cell monolayer. When the cells are detached, add Cell Culture Medium to inactivate

the typsin. Transfer the cells into a RNase-free tube and centrifuge at 300 xg for 5 min. Discard

supernatant carefully and go to lysis & homogenization (go to step 3).

3. (Lysis & homogenization) Add 50 µl (micro) / 400 µl (mini) / 2 ml (midi) of Buffer ① (Binding) to

each sample and mix thoroughly using a vortex mixer. Make sure that you must completely

resuspend the sample to achieve maximum lysis efficiency.

(Note) Insufficient homogenization can decrease the RNA purification yield, and also cause

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clogging of Magnetic Nano Beads in the following steps. For the sufficient homogenization of the

lysate, make the lysate passed through blunt 20-gauge needle (0.9 mm diameter) 5 to 10 times.

A. (Micro / Mini) please transfer the lysate to a 1.5 ml or 2 ml tube.

B. (Midi) please transfer the lysate to a 15 ml tube.

4. (RNA precipitation) Add 50 µl (micro) / 400 µl (mini) / 2 ml (midi) of absolute ethanol to the each

tube and mix well using a vortex mixer or by pipetting.

5. (RNA binding with Magnetic Nano Bead: 5- 7) Add 50 µl (micro) / 100 µl (mini) / 400 µl (midi) of

Magnetic Nano Bead solution to the tube and mix thoroughly using a vortex mixer until the beads

are fully resuspended.

(Note) Magnetic Nano Bead solution contains Magnetic Nano Beads. Please shake well or mix with

a vortex mixer before use.

6. Place the tubes on the MagListo™-2 (micro, mini) / MagListo™-15 (midi) Magnetic Separation

Rack with the magnet plate attached and invert the rack gently 3 to 4 times until the beads bind

tightly to the magnet.

- Attachment of the magnet plate

Combine the magnet plate to the stand.

7. Without removing the tube from MagListo™ Magnetic Separation Rack, discard the supernatant

carefully and completely remove the remaining supernatant on a paper towel action.

- How to discard the supernatant

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- Discard the supernatant by inverting the MagListo™ Magnetic Separation Rack. The silicone

immobilizer inside the stand prevents the tubes from falling. When discarding the supernatant, invert

the rack completely so that the solution does not to spill on the rack.

8. (1st washing: 8-10) Detach the magnet plate from MagListo™ Magnetic Separation Rack. Add 350

µl (micro) / 700 µl (mini) / 3.5 ml (midi) of Buffer ② (1st Washing) to the each tube and close the

cap. Mix by vortexing or shaking until the beads are fully resuspended.

-Detachment of the magnet plate

Detach the magnet plate gently by pulling it upwards.

9. Attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the

magnet.

10. Without removing the tubes from the MagListo™ Magnetic Separation Rack, discard the

supernatant and remove the remaining supernatant on a paper towel by blotting action.

11. (2nd washing) Repeat the steps 8 - 10 by adding 350 µl (micro) / 700 µl (mini) / 3.5 ml (midi) of

Buffer ③ (2nd Washing) instead Buffer ② for additional washing.

12. (3rd washing) Without removing the tubes from the MagListo™ Magnetic Separation Rack, add 700

μl (micro, mini) / 6 ml (midi) of Buffer ④ (3rd Washing) to “the opposite side of bead pellet”. Close

the cap and gently invert the rack twice in order to remove ethanol from the sample.

(Note) Direct pipetting of Buffer ④ onto the bead pellet, vortexing and/or vigorous shaking of the

tubes may release nucleic acid from the beads, which may result in lower RNA yield than expected.

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13. Discard the supernatant and completely remove the remaining supernatant by blotting action.

- Add Buffer ④ and discard the supernatant

14. (Elution: 14-18) Detach the magnet plate from the MagListo™ Magnetic Separation Rack. Add 50-

100 µl (micro, mini) / 250-500 µl (midi) of Buffer ⑤ (Elution) to the tube with the magnet plate

detached and resuspend completely by pipetting or vortex mixer for 15 sec.

15. Incubate the tube at 55℃ - 65℃ for 1 min.

16. Place the tubes on the MagListo™ Magnetic Separation Rack. Attach the magnet plate and invert the

rack gently 3 to 4 times until the beads bind tightly to the magnet.

17. Without removing the tubes from the MagListo™ Magnetic Separation Rack, transfer the

supernatant containing RNA to a new sterile microcentrifuge tube.

18. Discard the tubes with remaining Magnetic Nano Bead pellet. Do not reuse the beads.

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Summary of reagent volumes required in each step of Cell Total RNA Extraction

Step Buffer Micro Mini Midi Page

Lysis Buffer ① (Binding) 50 μl 400 μl 2 ml P. 10

RNA Precipitation Absolute Ethanol 50 μl 400 μl 2 ml P. 11

Bead Binding Magnetic Nano Bead 50 μl 100 μl 400 μl P. 11

1st Washing Buffer ② (1st Washing) 350 μl 700 μl 3.5 ml P. 12

2nd Washing Buffer ③ (2nd Washing) 350 μl 700 μl 3.5 ml P. 12

3rd Washing Buffer ④ (3rd Washing) 350 μl 700 μl 6 ml P. 12

Elution Buffer ⑤ (Elution) 50 - 100 μl 50 -100 μl 250 - 500 ul P. 13

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B. RNA Clean up (RNA Purification)

1. Transfer the eluted RNA or enzyme reaction product to a new sterile tube.

A. (Micro / Mini) Transfer the eluate to a 1.5 ml or 2 ml tube

B. (Midi) Transfer the eluate to a 15 ml tube

2. (Binding) Add 1 volume of Buffer ① (Binding) to 1 volume of the eluted RNA and mix completely

using a vortex mixer.

3. (RNA precipitation) Add 2 volumes of absolute ethanol to 1 volume of the eluted RNA and mix

completely using a vortex mixer.

4. (RNA binding with Magnetic Nano Bead: 5-7) Add 50 µl (micro) / 100 µl (mini) / 400 µl (midi) of

Magnetic Nano Bead solution to each tube and mix thoroughly using a vortex mixer until the beads

are fully resuspended.

(Note) Magnetic Nano Bead solution contains Magnetic Nano Beads. Please shake well or mix with

a vortex mixer before use.

5. Place the tubes on the MagListo™-2 (micro, mini) / MagListo™-15 (midi) Magnetic Separation

Rack with the magnet plate attached and invert the rack gently 3 to 4 times until the beads bind

tightly to magnet.

6. Without removing the tube from MagListo™ rack, carefully pour the supernatant out and completely

remove the remaining supernatant using paper towel by blotting action.

7. Go to step 8 of “A. RNA Extraction from Cultured Cell” in page 12 and follow the instructions

accordingly.

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C. One Step RNA Clean Up (DNase Treatment)

1. (RNA precipitation) Detach the magnet plate from the MagListo™ Magnetic Separation Rack. Add

400 µl (micro, mini) / 3.5 ml (midi) of absolute ethanol to the each tube and close the cap. Mix by

vortexing or shaking until the bead are fully resuspended

2. Place the tubes on MagListo™-2 (mirco, mini) / MagListo™-15 (midi) Magnetic Separation Rack

with the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the

magnet.

3. Without removing the tubes from the MagListoTM Magnetic Separation Rack, discard the

supernatant carefully and completely remove the remaining supernatant using a paper towel by

blotting action.

4. The beads can be dried with a dry oven at 65℃ for following times. (micro/mini : >5 min, midi :

>25 min) Please use a clean bench during the drying procedure to prevent RNase or other aerosol

contamination.

5. Add 50 μl of DNase Reaction Buffer and 10 μl of RNase-Free DNaseⅠ to the each tube.

6. Detach the magnet plate from the MagListo™ Magnetic Separation Rack and close the cap. Mix

using a vortex mixer until the beads are fully resuspended.

7. Place on the benchtop (20 - 30°C) for 20 min.

8. Detach the magnet plate from MagListo™ Magnetic Separation Rack. Add 350 µl (micro) / 700 µl

(mini) / 3.5 ml (midi) of Buffer ② (1st Washing) to the each tube and close the cap. Mix using a

vortex mixer until the beads are fully resuspended.

9. Place the tubes on the MagListo™ Magnetic Separation Rack with the magnet plate and invert the

rack gently 3 to 4 times until the beads bind tightly to the magnet.

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10. Without removing the tubes from the MagListo™ Magnetic Separation Rack, discard the

supernatant carefully and completely remove the remaining supernatant using a paper towel by

blotting action.

11. Go to step 11 of “A. RNA Extraction from Cultured Cell” in page 12 and follow the instructions

accordingly.

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XV. Appendix

Troubleshooting guide

This troubleshooting guide will help you to solve problem that may arise during RNA extraction. For

other technical assistance or more information, please contact our technical assistance team.

Comments and suggestions

Low yield of RNA

Buffers or other reagents may have been exposed to external factors that

may have reduced its quality. Please make sure that reagents are stored at

room temperature at all times upon arrival and that all reagent bottles are

closed tightly, in order to preserve pH and stability, and to avoid

contamination.

Too much amount of starting sample was used to extract RNA. Appropriate

amount of starting sample (see “Kit Specification” in page 4) should be

used.

Elution may have been incomplete. Please extend incubation time up to 3

minutes at elution step to improve the yield. In addition, make sure that

Magnetic Nano Beads are resuspended completely in the eluting solution

during incubation.

Some of Magnetic Nano Bead pellet may have been lost while discarding

solution. Check that all of the Nano Beads have bound tightly to the magnet

when you discard supernatant.

Insufficient shaking or vortexing during lysis step may lead to low RNA yield

than expected. Shake or mix the cell lysate with a vortex mixer sufficiently

during incubation step.

Cell culture medium may have been removed incomplete. Try to remove the

medium as much as possible. Any leftover in the medium can lead to the

inhibition of RNA extraction.

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Low A260/280 ratio

Beads may have been washed insufficiently. You must properly wash the

beads in the 3rd washing step. Remaining ethanol can decrease the purity of

RNA. Take enough time to wash the beads properly.

Incomplete suspension of beads during the washing step causes salts to

remain in the purified RNA. Make sure that the beads are suspended

thoroughly during the washing process.

Excessively clustered

Magnetic Nano Bead

Excess amount of starting sample is used to extract RNA. Appropriate

amount of starting material (see “Kit Specification” in page 4) should be used

for efficient extraction of RNA.

Presence of a white

precipitate in buffers

A white precipitate may form in Buffer ⓛ (Binding) due to prolonged storage

at low temperatures. Incubate Buffer ⓛ (Binding) at 60℃ until the

precipitate to be dissolved.

Degraded RNA

RNase contamination can be degraded RNA. Use a heat gun or a blow dryer

in a clean bench to prevent the contamination of RNase in the air. Use

RNase-free pipette tips and change the gloves frequently.

Frequent freezing and thawing may result in lower RNA yield than expected.

Avoid repeated freezing and thawing.

Flotation of extracted

RNA floats when loaded

on an agarose gel

Floating of RNA on an agarose gel is caused by the remaining ethanol in the

eluted RNA. Ensure that the 3rd Washing (ethanol removing) step in the

protocol is properly performed. Remaining ethanol may also interrupt the

enzymatic reaction.

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Experimental Data

Figure 1: Comparison of total RNA purified with MagListo™ 5M Cell Total RNA Extraction Kit and competitor

1x106 and 5x106 Hela cells were used for the comparison. Total RNA purification was performed with the

MagListo™ 5M Cell Total RNA Extraction Kit and the competitor’s product respectively. The bands of purified

total RNA were illustrated by 1% denaturing agarose gel electrophoresis. The equivalent level of purification

yield of the MagListo™ 5M Cell Total RNA Extraction Kit and the competitor’s product was confirmed through

the bands. Also the distinctive 28S/18S rRNA band patterns represent the superb quality of RNA purities having

no RNA degradations.

Figure 2: Extraction of RNA from various cell lines

1X106 of Huh7, Hela, 293T, and Balb/c 3T3 cells were used for the RNA purification performed with the

MagListo™ 5M Cell Total RNA Extraction Kit. The figure shows the bands of purified RNA made by using 1%

denaturing agarose gel electrophoresis. The effective RNA purifications from various cell lines could be

confirmed through the bands.

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Figure 3: RT-qPCR comparison of RNA isolated from 10 - 106 cells

(Left) Human GAPDH extracted from 10, 102, 103, 104,105, 106 Hela cells with the MagListo™ 5M Cell Total

RNA Extraction Kit were amplified using the AccuPower® RoketScript RT-qPCR Premix (K-6700) kit. The figure

represents fluorescent signals of amplified Human GAPDH.

(Right) The graph shows the comparison results of amplified Hela cell-GAPDH extracted with the MagListo™

5M Cell Total RNA Extraction Kit and the competitor’s product. According to the results, RNAs were

successfully purified from 10~106 Hela cells. Also the equivalent level of purification yield of the MagListo™ kit

and the competitor’s product was confirmed through the GAPDH Ct value of each cell number.

R² = 0.995

R² = 0.995

14.00

16.00

18.00

20.00

22.00

24.00

26.00

28.00

30.00

32.00

34.00

36.00

1.E+06 1.E+05 1.E+04 1.E+03 1.E+02 1.E+01

Ct

val

ue

Hela Cell-GAPDH

MagListo

Competitor

Cell Number

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XIV. Ordering Information

Cat no. Product Description Size

K-3601 MagListoTM 5M Plamid Extraction Kit, 100 reactions (mini) 1 kit

K-3600 MagListoTM 5M Plamid Extraction Kit, 500 reactions (mini) 1 kit

K-3603 MagListoTM 5M Genomic DNA Extraction Kit, 100 reactions (mini) 1 kit

K-3605 MagListoTM 5M Plant Genomic DNA Extraction Kit, 100 reactions (mini) 1 kit

K-3607 MagListoTM 5M Gel Extraction Kit, 100 reactions (mini) 1 kit

K-3609 MagListoTM 5M PCR Purification Kit, 100 reactions (mini) 1 kit

K-3611 MagListoTM 5M Cell Total RNA Extraction Kit, 100 reactions (mini) 1 kit

K-3613 MagListoTM 5M Tissue Total RNA Extraction Kit, 100 reactions (mini) 1 kit

K-3615 MagListoTM 5M Forensic Sample DNA Extraction Kit, 100 reactions (mini) 1 kit

K-3617 MagListoTM 5M Viral DNA / RNA Extraction Kit, 100 reactions (mini) 1 kit

K-3619 MagListoTM 5M Circulating Cell Free DNA Extraction Kit, 50 reactions (mini) 1 kit

TM-1000 MagListoTM-8Ch Magnetic Separation Rack 1 ml tube x 8 holes

TM-1010 MagListoTM-2 Magnetic Separation Rack 2 ml tube x 8 holes



HT-15-NG 1.5 ml microcentrifuge tube 500 ea / pk

HT-20-NG 2 ml microcentrifuge tube 500 ea / pk

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XIX. Explanation Symbols

Catalog

Number

Contains sufficient for

(n) tests USE BY

Batch code

Caution, consult

accompanying

documents

Temperature

Limitation

Manufacturer

Caution, Potential

Biohazard

DO NOT

REUSE

Consult Instruction

For Use

8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea

+82-42-930-8777 (Korea: 1588-9788)

+82-42-930-8688

[email protected]

www.bioneer.com

1301 Marina Village PKWY, Suite 110, Alameda, CA 94501, USA

+1-877-264-4300 (Toll-free)

+1-510-865-0350

[email protected]

us.bioneer.com

Korea Bio Park BLDG #B-702, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si

Gyeonggi-do, 13488, Republic of Korea

+82-31-628-0500

+82-31-628-0555

[email protected]

www.bioneer.co.kr

maglisto 5m cell total rna extraction kit - bioneer.co.kr magnetic nano beads enable the rapid...

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