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Liquid Chromatography AutosamplersProduct Line Overview

LC Autosampler (V10-EN).ppt

Copyright. All right reserved 2

Let’s know more about HTA

HT4000L

HT4000E: automated SPE – HPLC autosampler

Appendix

Agenda

Agenda

Copyright. All right reserved

I. Let’s know more about HTA

HTA: introduction

Leading automation provider for the scientific instruments industry. We have supplied a wide range of analyzer front-ends and sample preparation automated devices for 25+ years.

Most of our applications are in the analytical chemistry (chromatography) market, which is served indirectly by OEMs and directly by distributors. By OEM-only we also offers products for life science (capping, sealing and liquid handling add-ons), clinical (niche not-trivial products) and physical properties market.

We are committed to pursuing technical leadership, quality and service as key factors to succeed together with our customers. Our knowledge and experience of applications make our products optimized and cost effective.

A truly global company with a worldwide installed base: our headquarters and main facility is located in Brescia (Italy)within an intensive industrial area with a long tradition of automation techniques, electronics and mechanics.

Quality Management System certified UNI EN ISO 9001:2008



HT4000L

Overview

Key Features

Software

Configurations


Appendix

Agenda

Agenda


Overview

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II. HT4000L: Overview

HT4000L sits just aside the LC system with a very small bench space requirement.

The main module consists of an automatic liquid handler, a linear tray that handles samples in racks and an intuitive keypad for sample loading and basic operations.

We believe in “robust by design” approach: HT4000L – based on cutting edge technologies and DFX design – well incarnates this philosophy making itself the ideal autosampler for installation in any scenario.


Large sample capacity Feel free to use the sample vessel best fits your processes

176 2ml-vial capacity places HT4000L at the top of its category for sample capacity

Sample capacity together with pre-ahead capability, that brings delay time between 2 consecutive run as low as 12 seconds, make HT4000L the ideal instruments also for large routine labs: throughput and autonomy can be maximized without moving to expensive sample changer solution.

Manages samples out of vials (2/4/6/10/20/40 ml), test tubes, tube blocks or plates: choose from standard racks or enquiry for a custom design.

Custom design racks pay themselves in as low as few weeks of usage by allowing you important savings on sample reformatting operations

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II. HT4000L: Key Features


Application versatility

It is able to care about the widest range of liquid chromatography applications: analytical, prep, UHPLC, IC, nano… and many more!

Within the standard configuration the autosampler is equipped with an automatic 6-port 2-way vertical injection port valve for analytical applications.

However a wide selection of valves for inert or extended pressure application is also available as per special injection ports for applications where no valve is needed.

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Handles the most sophisticated sampling techniques. Parameters are easily programmable to optimize both the most convenient sampling method for extremely volatile or viscous samples and the best injection technique.

No compromise in term of solvent capacity and solvent selection: solvent can be located in vials (for accurate micro or nano volume dispensing) or in bottles (for large volume dispensing).


Application versatility: some examples

UHPLC READY UHPLC applications require an extended pressure range and a reduced amount of injected sample. HT4000L is optimized for such requirements and can be supplied with an UHPLC injection valve or can be upgraded in the field at a later time.

LARGE-VOLUME CAPABILITY

The only real autosampler choice in the market for large volume applications.We provide you with everything you need for your large volume applications: large sample vessel support, large injections up to 10ml, large solvent capacity, large bore size injection valve (or injection port) for real and complete automation.

BIOCOMPATIBLE To handle more complex samples and to assure covering a wider pH range, inert flow path valves are available. Biocompatible applications can take advantage of the double wash step capability: as pre- and post-wash solvent in addition to A, B… F solvents you can also choose a combination of A+B, A+C…. F+E solvents for superior analytical performance (carryover adverse!).

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Flexible and Modular (1/2)Endless possibilities!

A wide range of accessories can be sourced and added on the field at any time to implement HT4000L for maximum versatility of supported applications and for dealing with change scenario

Too much options? Don’t worry, you don’t pay for features you don’t need: only at the time your needs change you pay for the optional accessories to perform the new tasks without the hassle of buying a new autosampler.

Your investment is future proof!

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Flexible and Modular (2/2)Endless possibilities!

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Cooling optionThe cooling option offers the capability to maintain samples at a controlled temperature. The cooling option upgrade can be performed in the field in a very short time: you don’t need to make a decision about cooling at the time the autosampler is sourced!

Rack• 2ml vial (176 positions)• 4ml vial (96 positions)• 6/10/20ml vial (54 positions)• Cooled racks for superior thermal

conductivity• Custom racks for your own vessel or

mixed vessel types

Side Modules• Solvent vial, nozzles and bottle holders• Internal/External Needle Cleaning• Active Wash Station• Vortex• Reaction Cell/Heater• Bar Code Reader• …. And many more!

Liquid Handling• Syringes: from 500 nanoliter to 10 milliliter.

Special coating available for IC applications

• Peristaltic pumps for larger solvent dispensation


Lab Automation Ready (1/2)The Wow Factor: Get ready for the lab automation revolution!

HT4000L can fully automate your sample prep. Besides working as a standard HPLC autosampler, you may delegate some sample preparation tasks: HT4000L can take care of them as no standard autosampler has been able to assist.

Dilution, precolumn derivatization, pH spiking and internal standard addition are available within the standard version. These operations can be performed in place or in an empty vial by sample dispatching.

If standard sample prep capabilities are not enough get HTAPREP option that allow you to define any complex or custom autosampler method

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Lab Automation Ready (2/2)The Wow Factor: Get ready for the lab automation revolution!

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Standard Capability Advanced Capability(require HTAPREP)

No additional hardware module required

• Basic Dilution • Derivatisation• pH spiking • Internal standard addition• Sample displacement• Double wash• Prep ahead

• Advanced Dilution• Task (function) iterations• Serial, parallel or batch sample processing• Partial Injection• External events full control• Tracking by manual barcode reader• Handling of basic filtration and purification

procedure such as: Micro Extraction by Packed Sorbent (MEPS), Liquid/liquid extraction (LLE)

1 or more additional hardware module required

• Sample Cooling • Handling of rack with mixed vessels• Vessel displacement• Handling of advanced filtration and

purification procedure such as: Solid Phase Extraction (SPE), Whatman® Mini-UniPrep® syringeless vials, Syringe-filters

• Tracking by integrated a barcode reader• Vortex• Weigh by integrating a micro balance;• Uncap & Recap of screw type vials


A Robust Design

No needs for solvent degassing. While some autosamplers require to devote a degasser line or to degas offline wash solvent on each bottle refill we do not. Enjoy HT4000L!

Plumbing connections are visible and tubes run outside the instrument in a well organized manner so that leakage can be spotted out quickly and tube connection can be done easily without instrument disassembling.

At the same time sample reservoir bottles or vials are always visible: user is able to check if a bottle (a vial) is full or empty without removing it from its position

Preventive Maintenance counters inform operator about maintenance intervals in order to minimize and deeply reduce unscheduled out of service times

The on-board auto diagnosis tools allow to quickly spot the source of trouble. The module containing the failed component can be dismounted in a very quick and straight-forward way: it takes no more then 5 minutes. Then it can be sent back to a service center waiting for the repair. Uninstallation and re-installation can be done - in a large part of cases – directly from the customers.

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Press START and wait for data!

It features a keypad that consist in a combination of 4 keys and 5 status LEDs

All the routine operations such as analysis start, sample loading and extra wash can be managed by its dedicated keypad.

Set once your method by the complimentary HTA Autosampler Manager (Standard Version); then you just need to load samples and press START: experience a seamless integration with your Chromatography Data System.

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II. HT4000L: Software


Software Overview

HTA Autosampler Manager(Standard Version)

HTA Autosampler Manager(CFR 21 Part 11)

HTAPREP

Cost Included with the autosampler

$ $$$

Scope Allow autosampler programming (method &

sequence), installation and servicing.

Methods have a predefined structure: the order of the tasks that can be enabled or not is predefined, while

is possible to edit any relevant parameter.

The same of “HTA Autosampler Manager

(Standard Version)”

Include in additions CFR 21 Part 11 compliant

features

Allow autosampler programming (method &

sequence), installation and servicing.

Methods have NO predefined structure: the order of the tasks as any

parameters is programmable.

Complex sequence are also supported though a powerful runtime engine

Language English, Italian, Russian, Spanish

English, Italian, Russian, Spanish

English, Italian

Require software to stay always

on?

No (unless prep-ahead mode)

No (unless prep-ahead mode)

Yes



HTA Autosampler Manager

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HTA Autosampler Manager allows to perform autosampler setup, edit and download method; it may be also used to run a batch of samples. In additions it offers the following functionalities:


CFR 21 Part 11 (*) Report creation in PDF, CSV or XLS(X) Add name to methods & sequences Printing of relevant data Data Backup & Restore Remote Control (Virtual Terminal) Multi-language user interface

(*) “HTA Autosampler Manager” is provided for free. However CFR 21 Part 11 functionalities require to buy a software upgrade.



HTAPREP: Lab Automation Software

HTAPREP offers a brilliant software interface to assist creation and management from simple to complex routines for laboratory automation: Easy method creation by drag&drop Smart processing engine Sample List: automatic (through

integrated bar code reader) or assisted by the usage of manual barcode readers (i.e. barcode gun) or sample name variables (e.g. date, method name, etc.)

Multiple level access Software integration Advanced Usage Pattern


Chromatography Data System (CDS): For every software we offer integration!Compare below Basic and Advanced Integration at sample processing and autosampler programming

HOW IT WORKS?Basic IntegrationProvided for every CDS

Advanced IntegrationAvailable for some CDS only by purchasing extension or

driver at HTA or CDS vendor

Sample ProcessingThe activities you perform daily for processing your

samples.

Start you sample list with a “Double Start”

1) Load samples2) START the sample list from

your CDS3) Press START on autosampler

For the following samples in the sequence no additional

action required

Start you sample list with a “Single Start”

1) Load samples2) START the sample list from

your CDS

For the following samples in the sequence no additional

action required

ProgrammingTypically performed at installation or during autosampler method

development

Performed by keypad or HTA Autosampler Manager

Performed by keypad or HTA Autosampler Manager or

CDS

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Chromatography Data System (CDS) with ADVANCED Integration (1/2)

Clarityfor HT310L, HT4000L

ChemStation for LCChemStation for LC/MSD

EzChrom EliteOpenLAB CDS:

ChemStation Ed. for LCChemStation Ed. for LC/MSD

EzChrom Edition

for HT4000L


VWR-Clarityfor HT310L, HT4000L

EZChrom Elitefor HT4000L

OpenLAB CDSEzChrom Edition

for HT4000L


Chromatography Data System (CDS) with ADVANCED Integration (2/2)


YL-Clarityfor HT310L, HT4000L

ClarityChromfor HT310L, HT4000L

EZChrom Elitefor HT4000L

OpenLAB CDSEzChrom Edition

for HT4000L

LabSolutions

Lcsolution

LCMSsolution

for HT4000L


Welcome in the “Design by Me Era”Opt for one of the following option..

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II. HT4000L: Configurations

The easy choice

STANDARD CONFIGURATION

STANDARD CONFIGURATION +

ADDITIONAL MODULE

CUSTOM CONFIGURATION

Allows to customize the autosampler without

incurring in a customization fee

Select every component of your autosampler. A

small setup fee is charged


Standard Configuration

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HT4001LAnalytical

Configuration #1

HT4002LAnalytical

Configuration #2

HT4003LBiocompatible Configuration

HT4004LUHPLC

Configuration

HT4005LLarge Volume Configuration

HT4001LCCooling version

Tray 176 positions2ml vial

176 positions2ml vial





Syringe Default (Supported

range)

.1ml(0.1 – 10ml)

.5ml(0.1 – 10ml)

.5ml NeedlePTFE tipped(0.1 – 10ml)

.1ml(0.01 – 0.1ml)

2.5ml (0.1 – 10ml)

.1ml(0.1 – 10ml)

Solvent module(s)

4x10ml Vial 4x10ml Vial 4x10ml Vial 4x10ml Vial 3 Nozzle 7x10ml Vial

Waste module(s)

1x100ml Bottle 1x100ml Bottle

1x100ml Bottle

1x100ml Bottle

Infinite Wastew/internal &

external needle cleaning

1x100ml Bottle

Injection module(Max Pressure)

Valve: HPLC SS/6-2/0.40mm

(40Mpa)

Valve: HPLC SS/6-

2/0.40mm(40Mpa)

Valve: HPLC PEEK/6-

2/0.40mm (40Mpa)

Valve: UHPLC6-2/0.25mm(100Mpa)

Valve: HPLC SS/6-2/0.40mm

(40Mpa)

Loop 20µL (SS) 100µL (SS) 100µL (PEEK)

5µL (SS) 20µL (SS)

Besides the “6 standard configuration”, custom configurations are also available. Contact your sales rep to design Your Own Autosampler!

II. HT4000L: Configurations



HT4000L


Appendix

Agenda

Agenda


HT4000E: flexible HPLC autosampler that can automate your Solid Phase Extraction routine

Designed to automate SPE-LC(MS) applications, it takes care of sample purification up to the collection of eluted sample in vials (offline mode)or can proceed further up to the direct injection into the HPLC or LC-MS analyzer (online mode).

Do more than any HPLC autosampler

Easy SPE method design Constant flow elution technology Improve reproducibility and

throughput Reduce costs and delays

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IV. HT4000E


HT4000E: not only SPE Cartridges…

SPE Cartridges • Normal Phase

Compatible with most of the cartridges on the market• Reversed Phase

• Ion Exchange

• Affinity Enquiry

• Immunoaffinity

SLE Cartridges(Diatomaceous Earth Sorbent) Enquiry

Filter Cartridges Compatible with most of the cartridges on the market

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IV. HT4000E


HT4000L FAQ

HT4000L analytical performances

Appendix

Agenda


Focus: Push-to-fill approach

The injection technique is the most relevant aspect of an LC autosampler. While the 3 approaches available on the market have comparable results in terms of analytical performance, they differ deeply when looking at additional capabilities and user experience

We have evaluated this aspect carefully and opted for a push-to-fill approach that compared to pull-to-fill and integral loop looks a superior approach when considering: robustness, effectiveness of the washing system, variable injection volume and application versatility

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Most of the major LC manufacturers have a push-to-fill autosampler in their catalogue besides other injection-technique autosampler; in such case all of them agree to consider

the push-to-fill autosampler the top choice of their offering

Appendix – FAQ


Cooling Module: how cool is it?!

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Appendix – FAQ


How big is?!

HT4000L main body (21cm) is not wider than a standard column oven compartment, while side modules for a few handful of centimeters more offer extra capabilities and rooms for solvents and additional modules

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ShimadzuCTO-20A

WatersAcquity UPLC 30cm

Column Heater/Cooler

Appendix – FAQ


Lab automation: is not the same as CTC is doing?!

Nope! We are different from CTC and their VAR versions in 3 main aspects:

HT4000L has been designed as a bench-top instrument. It is not a GC autosampler put on a bench; it is not a compromise between a GC autosampler and a LC autosampler. You’ll recognize the difference when looking at footprint, installation and accessibility to sample area and to user interface

HTA Software are years ahead vs. their software (and their VAR software). Even if CTC software has received some major enhancements in 2013, the gap is still big: competitor software are based on macro, old technology and they are complex to program. Furthermore we are not on hold and by our continuous and short development cycle we are constantly committed to step up the competition barrier and maintain or increase the actual gap.

Upgradability cost. HT4000L asks you to pay for upgradability and additional modules only when these additional modules are required; by buying base unit you are not charged for upgradability. Our competitor technology is different: also when buying the base unit you start already paying for upgradability, even if you will never need

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Appendix – FAQ


Upselling IdeasYour sales experience continues year after year

Visit your HT4000L existing customers and check which applications are they running

Help them to achieve savings (e.g. by avoiding sample reformatting steps) and to improve the quantity and quality of their outputs (e.g. by automating sample preparation tasks).

Propose them to use optimized sample racks or to implement their autosampler with modules and options.

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Appendix – FAQ


How CHANGE may impact on your lab

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A modular and scalable autosampler enables laboratories to deal with change scenario.

For Contract labs and R&D labs, change is a need to complete a project/ a contract and to re-use assets for different assignments.

For QC labs, change is typically avoided and undesirable. Furthermore, change is perceived as a threat for asset obsolescence and not as being able to match new regulations or new manufacturing lines.

HT4000L can answer change requirements for Contract, R&D and QC labs offering them flexibility by growing or adapting with their mutating needs.

Appendix – FAQ


HT4000L, HT4000L + HTAPREP, HT4000A…

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Appendix – FAQ

HT4000L HT4000L+ HTAPREP option

HT4000A

Can work as Stand-alone HPLC

autosampler?

Yes Yes No

Can work as sample prep workstation?

No

(only limited sample prep capability provided)

Yes Yes

HTAPREP No Yes Yes

HTA Autosampler Manager

Yes Yes No

ClarityDataApex driver

Should be purchased at DataApex (included within

AS Module)

For “Stand-alone HPLC autosampler”

Purchased at DataApex (included within AS Module)

-For Sample prep workstation:

Included

Included w/HTAPREP

Replace the following HTA product

HT300L HT300LPlus HT400 seriesHT600 series


FAQ

HT4000L analytical performances

Appendix

Agenda


HT4000L analytical performance: published data

RSD (for volume ≥ 5ul) Full loop: ≤ 0.25% (typically 0.15%) Partial loop: ≤ 0.5%

Linearity (r): ≥ 0,9999

Carryover: typically ≤ 0.01% 1

Wait time between inj: as low as 12 sec2

In the next pages the results that have been achieved are shown, further below published data

(1) when using internal/external needle washing moduleTested with Uracil 100PPM in mobile phase 60% methanol : 40% water;

Column C18; detector: UV fixed length

(2) 10uL PL injection, with Prep Ahead Mode enabled

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Appendix – HT4000L analytical performances


HT4000L analytical performance: RSD Full LoopAnalytical volume

Instrument configurationSample loop: 20LSyringe size: 100L

MethodSample: Uracil 50ppm in waterWashing solvent: waterInj mode: full loopInj volume: 60lSample flush: 30l

HPLC system configurationPump: isocratic (flow: 0.5ml/min) Detector: UV fixed length (254nm) Column: noneDegasser

Sample Height Area

1 359,008 2489,465

2 359,258 2488,611

3 359,215 2486,791

4 358,969 2490,320

5 359,300 2487,885

6 359,089 2490,448

7 358,388 2485,720

8 358,858 2487,989

9 359,032 2488,626

Mean 359,013 2488,428

St. Dev. 0,275 1,554

RSD% 0,077 0,062

36



HT4000L analytical performance: RSD Partial LoopAnalytical volume


MethodSample: Uracil 50ppm in waterInj mode: partial loopInj volume: 10lSample flush: 30l

HPLC system configurationPump: isocratic (flow: 0.5ml/min) Detector: UV fixed length (254nm) Column: noneDegasser

Sample Height Area

1 656,223 4307,812

2 655,809 4304,378

3 656,881 4309,616

4 654,458 4298,952

5 654,357 4292,088

6 656,139 4307,586

7 655,915 4306,138

8 656,372 4302,269

9 657,933 4310,346

Mean 656,100 4304,354

St. Dev. 1,109 5,843

RSD% 0,169 0,136

37



HT4000L analytical performance: Linearity


MethodSample: uracil 50ppm in waterInj mode: partial loop Inj volume: 5, 10, 15lSample flush: 30l

HPLC system configurationPump: isocratic (flow: 0,5ml/min) Detector: UV fixed length (254nm) Column: noneDegasser

38


5ul

5ul

5ul

10ul

10ul

10ul

15ul

15ul

15ul

r

Sample Area

2113,059

2099,764

2104,832

4411,178

4413,004

4407,908

6679,163

6702,777

6849,620

0,999994


HT4000L analytical performance: Carryover (1/2)

Sample Height Area

Sample 283,176 3005,007

1° blank 0,121 1,056

2° blank 0,000 0,000

3° blank 0,000 0,000

4° blank 0,000 0,000

Ref std 0,05% 0,198 2,520

CO% 0,031 0,021

39



MethodSample: uracil 100ppm in methanol 60%, water 40%Reference Standard (Sample diluted 1:5000)Washing solvent: methanol 60%, water 40% Inj mode: partial loopInj volume: 10lWashes: pre wash, post wash, stator wash

HPLC system configurationPump: isocratic (flow: 1ml/min) Detector: UV fixed length (254nm) Column: C18Degasser


HT4000L analytical performance: Carryover (2/2) Using the internal/external needle washing module

Sample Height Area

Sample 884,781 19875,690

1° blank 0,191 1,343

2° blank 0,204 1,520

3° blank 0,186 1,413

4° blank 0,000 0,000

Ref std 0,01% 0,359 2,631

CO% 0,005 0,005

40


Instrument configurationSample loop: 20LSyringe size: 100LModule: Internal/external needle washing

MethodSample: uracil 500ppm in methanol 60%, water 40%Reference Standard (Sample diluted 1:10000)Washing solvent: methanol 60%, water 40% Inj mode: partial loopInj volume: 10lWashes: using internal/external needle washing module

HPLC system configurationPump: isocratic (flow: 1ml/min) Detector: UV fixed length (254nm) Column: C18Degasser

Copyright. All right reserved 4141Via del Mella, 77 – 25131 Brescia – Italy

T:+39 030 3582920 – F: +39 030 3582930 – E: [email protected] – W: www.hta-it.com

HTA: Sampling for scienceThanks for your kind attention

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