lc columns - satia · lc columns uslc® columns evaluating and extending selectivity the restek...

LC ColumnsUSLC® Columns .......................................................137–139U.S. Pharmacopeia Cross-Reference .........................140Tables and Reference ......................................................141Columns for UHPLC ...............................................142–148Columns for HPLC ..................................................149–154Columns for Bioseparations ...............................155–157Application-Specific Columns ...........................158–163Prep Columns ...........................................................164–167Bulk Packing Materials....................................................167UHPLC Filters ......................................................................168HPLC Guard Cartridge Systems ........................169–171LC Column & Detector Test Mixes .............................449LC Chromatograms................................................690–721

www.restek.com 137

LC COLUMNS

USLC® Columns

Ultra Selective Liquid Chromatography Technology

Choose Columns Fast. Develop Methods Faster.What is Ultra Selective Liquid Chromatography (USLC®) technology? This technique is the directed application of orthogonal selectivity—the most influential factor affecting peak separation, or resolution—to provide the practicing chromatographer with the best tools for choosing columns fast and developing methods faster. Through our extensive study of reversed-phase chromatographyRestek created the widest range of selectivity in the industry using just four unique stationary phases: the USLC® column set. We also defined a simple approach to choosing a column with the appropriate selectivity for any application.

Selectivity Drives SeparationsQuickly and effectively resolve analytes by understanding and controlling selectivity through USLC® technology.One of the most significant, yet least understood, steps of method development is finding the proper stationary phase for a par-ticular separation. As sample complexity increases, achieving adequate resolution between matrix components and target analytes becomes more difficult. Despite recent advancements in column format, such as sub-2-micron packings and pellicular particles, resolution can still be difficult to obtain because, while these formats can increase chromatographic efficiency and analysis speed, they do not significantly influence resolution. Selectivity, as shown in Equation 1, is the single most powerful factor affecting resolution, and it is largely dependent upon sta-tionary phase composition.

Real Diversity in Phase ChemistryA small set of defined orthogonal columns means faster separations and more robust methods.While numerous bonded phases are available for reversed-phase chromatography, many (e.g., C8 and C18) are similar and offer only moderate changes in retention, rather than significant differences in selectivity. Method development is less laborious and time-consuming when you use a full range of column selectivities, including orthogonal phase chemistries like polar-embedded, phenyl,and fluorophenyl columns. Restek has led the development of the unique USLC® column set across these phase classes to provide analysts with a more effective range of column selectivities and innovative column chemistries for method development. The USLC®column set (Figure 1) provides the widest range of reversed-phase selectivity available with just four columns and can be used to guide proper stationary phase selection—the least understood yet most significant part of method development.

Figure 1: Restek columns offer the widest range of unique and effective phase chemistries to aid the chromatographer in choosing columns fast and developing methods faster.

Restek USLC®

Phase

(column class)

Aqueous C18 (alkyl) Biphenyl (phenyl) IBD (polar embedded) PFP Propyl (fluorophenyl)

Ligand Type Proprietary polar modified and functionally bonded C18

Unique Biphenyl Proprietary polar functional embedded alkyl

Fluorophenyl

Properties • General-purpose with a well-balanced retention profile.

• Compatible with 100% aqueous mobile phases.

• Ideal for multi-component LC-MS analyses.

• Increased retention for dipolar, unsaturated, or conjugated solutes.

• Enhanced selectivity when used with protic (methanol) mobile phase.

• Ideal for increasing sensitivity and selectivity in LC-MS analyses.

• Increased retention for acids and water-soluble compounds.

• Compatible with 100% aqueous mobile phases.

• Capable of both reversed-phase and HILIC separations.

• Increased retention for both charged bases and electronegative compounds.

• Capable of both reversed-phase and HILIC separations.

• Ideal for increasing sensitivity and selectivity in LC-MS analyses.

Aqueous C18

Si

OO

P

IBD

CH3 CH3

O

Si

P

Biphenyl

SiCH3 CH3

O

PFP Propyl

SiCH3 CH3

O

FF

FF

F

Equation 1: Selectivity has the greatest mathematical effect on resolution.

R = ¼ N x (k/[k+1]) x (α-1)Efficiency Retention Factor Selectivity

RESTEK USLC®® Ultra Selective Liquid Chromatographywww.restek.com/uslc

138 www.restek.com

Figure 2: Restek has extended the selectivity range for commercially available columns and defined a column set—the four USLC® phases—that is ideal for fast column choice and faster method development.

LC COLUMNS

USLC® Columns

Evaluating and Extending SelectivityThe Restek USLC® column set offers the highest range of alternate selectivity available.Th e diverse selectivity provided by USLC® columns can be demonstrated empirically using the hydrophobic-subtraction model [1]. Th is model is a novel procedure for characterizing selectivity that uses test probes to defi ne the solute and stationary phase interac-tions in reversed-phase separations. Restek is leading the commercial application of this model by implementing it in the develop-ment of USLC® bonded phases. To evaluate phase selectivity using the hydrophobic-subtraction model, the retention characteristics of the solute probes are compared across diff erent phases relative to a C18 benchmark with all columns using the same silica base.

Th e resulting scatter plot is an excellent way to visualize selectivity. Stationary phases with similar selectivity show high linearity when graphed. However, stationary phases with alternate selectivity—even orthogonality—produce signifi cant scatter around the regression line. Th e high degree of scatter shown in Figure 2 shows just how diverse the phases in the USLC® column set are. When we quantify column selectivity based on this correlation by calculating the selectivity (S) statistic [2], the resulting value of 46.7 shows that the USLC® column set truly has the highest range of selectivity available.

All columns were tested using the same silica support.

Orthogonal phases14.00

12.00

10.00

8.00

6.00

4.00

2.00

k Ort

hogo

nal p

hase

0.000.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

k C18 phase

y = 0.4755x + 0.7619R2 = 0.7819

S = 46.7

Aqueous C18

IBD

Biphenyl

PFPP

USLC® Column Interaction Profile

Put simply, selectivity is the retention of one compound relative to another. Therefore, because solutes will be retained to different degrees by different molecular interactions, we can fundamentally define a column’s selectivity based on the molecular interactions it delivers.

Each USLC® column is optimized for a different chemical interaction. The pie chart provided for each USLC® stationary phase in this catalog (Figure 3) identifies the same four molecular interactions (color coded to correspond to the retention of a different solute type). The more rings shown for a given interaction, the more significant a role it plays in defining solute retention.

If you know what type of column interaction you need for your analysis, use these charts to select your USLC® column.

Figure 3: Sample USLC® Column Interaction Profile

Disp

ersion H-Bonding

Polarizability Cation Exchan

ge

References[1] L.R. Snyder, J.W. Dolan, P.W. Carr, The Hydrophobic-Subtraction Model of Reversed-Phase Column Selectivity, J. Chromatogr. A 1060 (2004) 77.[2] U.D. Neue, J.E. O’Gara, A. Mendez, Selectivity in Reversed-Phase Separations influence of the Stationary Phase, J. Chromatogr. A 1127 (2006) 161.

www.restek.com 139

LC COLUMNS

USLC® Columns

USLC® Method Development Toolbox • Ultra Selective Liquid Chromatography (USLC®) method development toolbox

contains all four USLC® stationary phases in one convenient package.• Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm

lengths.• Included selection guide makes it even easier to pick the right column the fi rst time.

Description Size Includes qty. cat.# Pinnacle DB USLC Method Development Toolbox

1.9 μm, 2.1 mm x 50 mm

(1) each: Biphenyl (9409252), Aqueous C18 (9418252), IBD (9425252), PFP Propyl (9419252) kit 25800

Pinnacle DB USLC Method Development Toolbox

1.9 μm, 2.1 mm x 100 mm


Ultra USLC Method Development Toolbox

3 μm, 2.1 mm x 50 mm



3 μm, 2.1 mm x 100 mm



3 μm, 3.0 mm x 100 mm

(1) each: Biphenyl (910931E), Aqueous C18 (917831E), IBD (917531E), PFP Propyl (917931E) kit 25803


5 μm, 2.1 mm x 50 mm



5 μm, 2.1 mm x 100 mm



5 μm, 4.6 mm x 150 mm


USLC® Columns Choose Columns Fast. Develop Methods Faster.

www.restek.com/uslc

Mobile Phase Management 101

Reduce Solvent Waste

Last Drop FilterSee page 320.

Prepare and Maintain Mobile Phases Without Dissolved Gas or Unnecessary Costs

Mobile Phase Sparge FilterSee page 320.

Neatly Keep Mobile Phase Lines Where They Belong

Hub-CapSee page 318.

Avoid Messy Spills Around Mobile Phase Waste Containers

Waste Overflow

IndicatorSee page 319.

Transfer and Filter Mobile Phase in a Single Step

Hub-Cap FiltersSee page 319.

Hub-Cap (assembly of the bott le cap and plug)

140 www.restek.com

LC COLUMNS

U.S. Pharmacopeia Cross Reference

U.S. Pharmacopeia Cross Reference

EXP®

fittings hand-tight

Reusable fittings for easy, yet reliable HPLC & UHPLC connections

www.restek.com/exp

See page 313.

• Hand-tight fitting style achieves effortless HPLC seals—no tools needed!

• Both hand-tight and hex-head styles wrench-tighten for reliable UHPLC use.

• Patented ferrule can be installed repeatedly without compromising high-pressure seal.

• Hybrid design combines the durability of titanium with the sealing ability of PEEK.

• Cutting-edge system provides ZDV (zero dead volume) connection to any 10-32 female port.

• Compatible with 1/16" PEEK and stainless steel tubing.

hex-head

L1Octadecyl silane chemically bonded to porous silica or ceramic microparticles; 1.7 to 10 μm in diameter, or a monolithic rod. Pinnacle® DB C18 (p. 143 ), Pinnacle® DB Aqueous C18 (p. 144 ), Ultra C18 (p. 149 ), Ultra Aqueous C18 (p. 151 ), Viva C18 (p. 155 )

L3Porous silica particles; 5 to 10 μm in diameter. Pinnacle® DB Silica (p. 147 ), Ultra Silica (p. 154 ), Viva Silica (p. 157 )

L7Octylsilane chemically bonded to totally porous silica particles; 1.7 to 10 μm in diameter. Pinnacle® DB C8 (p. 143 ), Ultra C8 (p. 149 ), Viva C8 (p. 155 )

L8An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support; 3 to 10 μm in diameter. Ultra Amino (p. 154 )

L10Nitrile groups chemically bonded to porous silica particles; 3 to 10 μm in diameter. Pinnacle® DB Cyano (p. 147 ), Ultra Cyano (p. 154 )

L11Phenyl groups chemically bonded to porous silica particles; 1.7 to 10 μm in diameter. Pinnacle® DB Biphenyl (p. 145 ), Ultra Aromax (p. 151 ), Ultra Biphenyl (p. 152 ), Viva Biphenyl (p. 156 )

L13Trimethylsilane chemically bonded to porous silica particles; 3 to 10 μm in diameter. Ultra C1 (p. 150 )

L26Butyl silane chemically bonded to totally porous silica particles; 3 to 10 μm in diameter. Ultra C4 (p. 150 ), Viva C4 (p. 156 )

L43Pentafl uorophenyl groups chemically bonded to silica particles by a propyl spacer; 5 to 10 μm in diameter. Pinnacle® DB PFP Propyl (p. 146 ), Ultra PFP Propyl (p. 153 ), Viva PFP Propyl (p. 156 )

L68Spherical, porous silica; 100 μm or less in diameter, the surface of which has been covalently modifi ed with alkyl amide groups and not end-capped.Pinnacle® DB IBD (p. 146 ), Ultra IBD (p. 153 )

www.restek.com 141

Common Classifi cations for LC

Columns by Internal Diameter

LC COLUMNS

Tables and Reference

Classifi cation Internal DiameterCapillary <1.0 mm IDMicro bore 1.0 mm IDNarrow bore 2.1–3.0 mm IDStandard bore 3.2–4.6 mm IDSemi-prep 10–21.2 mm IDPrep 30–50 mm ID

Optimal Flow Rates for Various Particle Diameters

and Column Internal Diameters

Column ID Optimal fl ow rate

(mm) (mL/min)

1.9 μm dp 3 μm dp 5 μm dp 4.6 — 1.50 1.00 3.2 — 0.73 0.50 3.0 1.12 0.65 0.40 2.1 0.55 0.31 0.20 1.0 — 0.07 0.05

Do we have the reference info for this????

Solvent Miscibility and Solubility

AcetoneAcetonitrile

Dimethyl formamideDimethyl sulfoxide

DioxaneEthanol

Isopropyl alcoholMethanol

TetrahydrofuranWater

BenzeneButanol

Carbon tetrachlorideChloroform

Cyclohexane1,2-Dichloroethane

DichloromethaneEthyl acetate

Ethyl etherHeptane

HexaneIsooctane

Methyl tert-butyl etherMethyl ethyl ketone

PentaneToluene

Xylene

Miscible/Water-Miscible Solvents

Miscible Solvents

Immiscible Solvents

Aceton

eAce

tonitr

ileDim

ethyl

formam

ide

Dimeth

yl su

lfoxid

eDiox

ane

Ethan

olIso

propy

l alco

hol

Methan

olTe

trahy

drofur

anWate

rBen

zene

Butano

lCarb

on te

trach

loride

Chlorof

ormCyc

lohex

ane

1,2-D

ichlor

oetha

ne

Dichlor

ometh

ane

Ethyl

aceta

teEth

yl eth

erHep

tane

Hexan

eIso

octan

eMeth

yl ter

t-buty

l ethe

r

Methyl

ethyl

keton

e

Penta

neTo

luene

Xylene

To convert a pressure, multiply the units in the left-most column by the conversion factors listed in the columns to the right.For example: 10 psi x 0.068 = 0.68 atm 10 bar x 29.5300 = 295.300 inches Hg

HPLC Pump Pressure Conversion Table

Pressure psi atm kg/cm2 torr kPa bar inches Hg 1 psi = 1 0.068 0.0703 51.713 6.8948 0.06895 2.0359 1 atm = 14.696 1 1.0332 760 101.32 1.0133 29.921 1 kg/cm2 = 14.223 0.967 1 735.5 98.06 0.9806 28.958 1 torr = 0.0193 0.00132 0.00136 1 0.1330 0.00133 0.0394 1 kPa = 0.1450 0.00987 0.0102 7.52 1 0.0100 0.2962 1 bar = 14.5038 0.9869 1.0197 751.88 100 1 29.5300 1 in Hg = 0.49612 0.0334 0.0345 25.400 3.376 0.03376 1

142 www.restek.com

LC COLUMNS

Columns for UHPLC

Pinnacle® DB Columns: 1.9, 3, or 5 μm particles; 140 Å pore sizeRestek Pinnacle® DB columns are built for optimal UHPLC performance.

Pinnacle® DB columns are 100% manufactured by Restek in our Bellefonte, Pennsylvania, facility. Because performance begins with the support, our Pinnacle® DB UHPLC columns start with base-deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety of phases to give chromatographers a stable and selective column. Get the most out of your UHPLC system. Combine selectivity and effi ciency by using Restek Pinnacle® DB UHPLC columns.

Restek-manufactured silica

www.restek.com/LC-Columns

Get Even More Out of Your UHPLC SystemCombine UHPLC Effi ciency With Restek Selectivity

USLC® Columns:

Phase Page

Aqueous C18 (USLC phase) ............144Biphenyl (USLC Phase) ......................145IBD (USLC Phase) ................................146PFP Propyl (USLC Phase) ..................146

Conventional Columns:

Phase Page

C18 ...........................................................143C8 .............................................................143Cyano ......................................................147PAH ..........................................................148Silica ........................................................147

The following stationary phases are available for UHPLC:

www.restek.com 143

also available

Trident Direct Guard Column System

See page 169.

LC COLUMNS

Columns for UHPLC

Chromatographic P roperties

Th e general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic.

Pinnacle® DB C18 Columns (USP L1)

Looking for an equivalent column?Restek has extensively studied column selectivity and can provide you with an accurate recommendation. Please contact Restek Technical Support or your local Restek representative.

1.0 mm ID 2.1 mm ID 3.0 mm ID 4.6 mm IDLength cat.# cat.# cat.# cat.#

1.9 μm Columns 30 mm 9414232 50 mm 9414252

100 mm 9414212 3 μm Columns

30 mm 9414331 9414332 941433E 9414335 50 mm 9414351 9414352 941435E 9414355

100 mm 9414311 9414312 941431E 9414315 5 μm Columns

30 mm 9414531 9414532 941453E 9414535 50 mm 9414551 9414552 941455E 9414555

100 mm 9414511 9414512 941451E 9414515 150 mm 9414561 9414562 941456E 9414565 200 mm 9414521 9414522 941452E 9414525 250 mm 9414571 9414572 941457E 9414575



Our C8 is a conventional monomeric octylsilane column off ering a shorter alkyl chain to pro-vide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic.

Pinnacle® DB C8 Columns (USP L7)


1.9 μm Columns 30 mm 9413232 50 mm 9413252


30 mm 9413331 9413332 941333E 9413335 50 mm 9413351 9413352 941335E 9413355

100 mm 9413311 9413312 941331E 9413315 5 μm Columns

30 mm 9413531 9413532 941353E 9413535 50 mm 9413551 9413552 941355E 9413555

100 mm 9413511 9413512 941351E 9413515 150 mm 9413561 9413562 941356E 9413565 200 mm 9413521 9413522 941352E 9413525 250 mm 9413571 9413572 941357E 9413575

Column Characteristics: particle size: 1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °CUSP phase code: L7phase category: C8, octylsilaneligand type: monomeric C8

SiCH3 CH3

O

C8


Column Characteristics: particle size: 1.9 μm, 3 μm, or 5 μm, sphericalpore size: 140 Å carbon load: 11% end-cap: yes pH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: C18, octadecylsilaneligand type: monomeric C18

C18

SiCH3 CH3

O

144 www.restek.com


1.9 μm Columns 30 mm 9418232 50 mm 9418252


30 mm 9418331 9418332 941833E 9418335 50 mm 9418351 9418352 941835E 9418355

100 mm 9418311 9418312 941831E 9418315 150 mm 9418361 9418362 941836E 9418365

5 μm Columns 30 mm 9418531 9418532 941853E 9418535 50 mm 9418551 9418552 941855E 9418555

100 mm 9418511 9418512 941851E 9418515 150 mm 9418561 9418562 941856E 9418565 200 mm 9418521 9418522 941852E 9418525 250 mm 9418571 9418572 941857E 9418575

Chromatographic Properties

Th e Restek Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profi le. It can eff ectively retain more types of solutes than a conventional C18 and is ideal for multi-component LC-MS analyses. Th e general-purpose Aqueous C18 boasts high reproduc-ibility and compatibility with many mobile phase conditions—even 100% aqueous and acidic. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time.

Pinnacle® DB Aqueous C18 Columns (USP L1)

LC COLUMNS

Columns for UHPLC

Column C haracteristics: particle size: 1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% pH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: modifi ed C18ligand type: proprietary polar modifi ed and functionally bonded C18

Pesticides on Pinnacle® DB Aqueous C18 (LC-MS/MS, ESI+)

LC_FF0479A

Column Pinnacle® DB Aqueous C18 (cat.# 9418252)

Dimensions: 50 mm x 2.1 mm IDParticle Size: 1.9 μmPore Size: 140 ÅTemp.: 35 °CSample multicomponent pesticide

standardDiluent: waterConc.: 33.3 ppb each pesticideInj. Vol.: 5 μLMobile Phase A: 10 mM NH4OAc in waterB: 10 mM NH4OAc in methanol Time (min) %B 0 10 1 10 8 90 10 90 11 10Flow: 0.60 mL/minMax Pressure: ~517 barDetector Applied Biosystems/MDS

Sciex LC-MS/MSModel #: 4000 QTRAP® LC-MS/MS

systemIon Source: TurboIonSpray®Ion Spray Voltage: 5 kVGas 1: 40 psi (275.8 kPa)Gas 2: 60 psi (413.7 kPa)Source Temp.: 500 °CInstrument Shimadzu UFLCXR

➲ See page 717 for compound listing.

Disp

ersion H-Bonding


ge

USLC® Column Interaction Profi le(See page 138 for more information.)

Aqueous C18

Si

OO

P


www.restek.com 145

Sulfur Antibiotics by UHPLC on Pinnacle® DB Biphenyl

LC COLUMNS

Columns for UHPLC


Th e Restek Biphenyl off ers a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial in-creases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses.

Pinnacle® DB Biphenyl Columns (USP L11)


1.9 μm Columns 30 mm 9409232 50 mm 9409252


30 mm 9409331 9409332 940933E 9409335 50 mm 9409351 9409352 940935E 9409355

100 mm 9409311 9409312 940931E 9409315 150 mm 9409361 9409362 940936E 9409365


100 mm 9409511 9409512 940951E 9409515 150 mm 9409561 9409562 940956E 9409565 200 mm 9409521 9409522 940952E 9409525 250 mm 9409571 9409572 940957E 9409575

LC_GN0532

Column Pinnacle® DB Biphenyl (cat.# 9409212)Dimensions: 100 mm x 2.1 mm IDParticle Size: 1.9 μmPore Size: 140 ÅTemp.: 25 °CSampleDiluent: 0.1% Formic acid in waterConc.: 50 μg/mLInj. Vol.: 2 μLMobile Phase A: 0.1% Formic acid in waterB: 0.1% Formic acid in acetonitrile Time (min) %A %B 0 95 5 8 60 40Flow: 0.4 mL/minDetector UV/Vis @ 265 nmInstrument Shimadzu UFLCXR

Peaks RT (min) 1. Sulfanilamide 1.55 2. Sulfadiazine 3.74 3. Sulfapyridine 4.09 4. Sulfathiazole 4.24 5. Sulfamerazine 4.35 6. Sulfamethazine 4.91 7. Sulfachlorpyridazine 5.87 8. Sulfadoxine 5.99 9. Sulfi soxazole 6.37 10. Sulfadimethoxine 7.14 11. Sulfaquinoxaline 7.40


Column Characteristics: particle size: 1.9 μm, 3 μm, or 5 μm, sphericalpore size: 140 Åcarbon load: 8%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase: L11phase category: phenylligand type: unique Biphenyl

Disp

ersion H-Bonding


ge


Biphenyl

SiCH3 CH3

O

146 www.restek.com

LC COLUMNS

Columns for UHPLC


Th e Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases.

Pinnacle® DB IBD UHPLC Columns (USP L68)

2.1 mm IDLength cat.# 1.9 μm Columns 30 mm 9425232 50 mm 9425252 100 mm 9425212

Disp

ersion H-Bonding


ge

Column C haracteristics: particle size: 1.9 μm, sphericalpore size: 140 Å end-cap: no pH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L68phase category: polar-embedded alkylligand type: proprietary polar functional embedded alkyl

IBD


CH3 CH3

O

Si

P



1.9 μm Columns 30 mm 9419232 50 mm 9419252


30 mm 9419331 9419332 941933E 9419335 50 mm 9419351 9419352 941935E 9419355

100 mm 9419311 9419312 941931E 9419315 150 mm 9419361 9419362 941936E 9419365


100 mm 9419511 9419512 941951E 9419515 150 mm 9419561 9419562 941956E 9419565 200 mm 9419521 9419522 941952E 9419525 250 mm 9419571 9419572 941957E 9419575

Column C haracteristics: particle size: 1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% end-cap: yes pH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L43phase category: fl uorophenyl propylligand type: pentafl uorophenyl propyl

Disp

ersion H-Bonding


ge


PFP Propyl

SiCH3 CH3

O

FF

FF

F


Th e Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, however, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and effi cient with acidic mobile phases. Th is versatile column is also compatible with highly aqueous mobile phases and HILIC separations.

Pinnacle® DB PFP Propyl Columns (USP L43) Restek-manufactured silica

www.restek.com 147

LC COLUMNS

Columns for UHPLC


Th e Restek Cyano is a traditional monomeric cyanopropylsilane that is recommended for assays where alternate selectivity, or confi rmation, to a C18 or C8 column is desired. It can be used in normal-phase, reversed-phase (best with mobile phase pH between 5 and 7), and HILIC modes. It is an excellent choice for the analysis of protonated bases.

Pinnacle® DB Cyano Columns (USP L10)


1.9 μm Columns 30 mm 9416232 50 mm 9416252


30 mm 9416531 9416532 941653E 9416535 50 mm 9416551 9416552 941655E 9416555

100 mm 9416511 9416512 941651E 9416515 150 mm 9416561 9416562 941656E 9416565 200 mm 9416521 9416522 941652E 9416525 250 mm 9416571 9416572 941657E 9416575

Column Characteristics: particle size: 1.9 μm or 5 μm, spherical pore size: 140 Åcarbon load: 4% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °CUSP phase code: L10phase category: cyanoligand type: cyanopropylsilane

Cyano

SiCH3

NC

CH3

O



Base-deactivated spherical silica is useful for normal-phase or HILIC separations.

Pinnacle® DB Silica Columns (USP L3)


1.9 μm Columns 30 mm 9410232 50 mm 9410252


30 mm 9410331 9410332 941033E 9410335 50 mm 9410351 9410352 941035E 9410355

100 mm 9410311 9410312 941031E 9410315 150 mm 9410361 9410362 941036E 9410365


100 mm 9410511 9410512 941051E 9410515 150 mm 9410561 9410562 941056E 9410565 200 mm 9410521 9410522 941052E 9410525 250 mm 9410571 9410572 941057E 9410575

Column Characteristics: particle size: 1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å end-cap: no pH range: 2.5 to 8 temperature limit: 80 °CUSP phase code: L3phase category: bare silicaligand type: none Silica

Si

OO O

OH


148 www.restek.com

LC COLUMNS

Columns for UHPLC


Specifi cally designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Get complete resolution of all 16 EPA 610 PAHs, plus two other routinely analyzed PAH com-pounds, in less than fi ve minutes to greatly reduce run times and increase sample throughput.

Pinnacle® DB PAH UHPLC Columns



Get all four USLC® stationary phases in one convenient package.

• Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.

• Included selection guide makes it even easier to pick the right column the first time.

See page 139.

All the Right Tools—All in One Toolbox

www.restek.com/uslc

PAH Mix on Pinnacle® DB PAH

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00Time (min)

1 2 3 4 5

6

7

8

9 10

11

12

13

1415

1617

18

LC_EV0521

Peaks 1. Naphthalene 2. Acenaphthylene 3. 1-Methylnaphthalene 4. 2-Methylnaphthalene 5. Acenaphthene 6. Fluorene 7. Phenanthrene 8. Anthracene 9. Fluoranthene 10. Pyrene 11. Benzo[a]anthracene 12. Chrysene 13. Benzo[b]fl uoranthene 14. Benzo[k]fl uoranthene 15. Benzo[a]pyrene 16. Dibenzo[a,h]anthracene 17. Benzo[ghi]perylene 18. Indeno[1,2,3-cd]pyrene

Column Pinnacle® DB PAH (cat.# 9470252)

Dimensions: 50 mm x 2.1 mm IDParticle Size: 1.9 μmPore Size: 140 ÅTemp.: 30 °CSample EPA Method 8310 PAH Mixture

(cat.# 31841)Diluent: acetonitrileConc.: 10 μg/mLInj. Vol.: 1 μL

Mobile Phase A: waterB: acetonitrile Time (min) Flow (mL/min) %A %B 0 0.8 60 40 2 0.8 40 60 4 0.8 0 100 4.5 0.8 0 100 4.51 0.8 60 40 5 0.8 60 40Max Pressure: 724 barDetector Photo diode array @ 254, 4.8 nmInstrument Waters

Column C haracteristics: particle size: 1.9 μm, sphericalpore size: 140 Å end-cap: no pH range: 2.5 to 8temperature limit: 80 °C

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LC COLUMNS

Columns for HPLC

Ultra HPLC Columns: 3 or 5 μm particles; 100 Å pore sizeTh e Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, these columns are designed for selective and reliable HPLC applications.



Ultra C8 Columns (USP L7)

1.0 mm ID 2.1 mm ID 3.0 mm ID 4.0 mm ID 4.6 mm IDLength cat.# cat.# cat.# cat.# cat.#


100 mm 9103311 9103312 910331E 9103315 150 mm 9103361 9103362 910336E 9103365


100 mm 9103511 9103512 910351E 9103514 9103515 150 mm 9103561 9103562 910356E 9103564 9103565 200 mm 9103521 9103522 910352E 9103525 250 mm 9103571 9103572 910357E 9103575

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 12%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L7phase category: C8, octylsilaneligand type: monomeric C8

SiCH3 CH3

O

C8






100 mm 9174311 9174312 917431E 9174315 150 mm 9174361 9174362 917436E 9174365


100 mm 9174511 9174512 917451E 9174514 9174515 150 mm 9174561 9174562 917456E 9174564 9174565 200 mm 9174521 9174522 917452E 9174525 250 mm 9174571 9174572 917457E 9174575

C18

SiCH3 CH3

O

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 20%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: C18, octadecylsilaneligand type: monomeric C18

150 www.restek.com

LC COLUMNS

Columns for HPLC


Exceptionally stable C4 packing with high bonding coverage and base deactivation. Less retention than C18 or C8 phases.




100 mm 9102311 9102312 910231E 9102315 150 mm 9102361 9102362 910236E 9102365


100 mm 9102511 9102512 910251E 9102515 150 mm 9102561 9102562 910256E 9102565 200 mm 9102521 9102522 910252E 9102525 250 mm 9102571 9102572 910257E 9102575


Exceptionally stable C1 phase. Least retentive reversed-phase hydrocarbon packing.




100 mm 9101311 9101312 910131E 9101315 150 mm 9101361 9101362 910136E 9101365


100 mm 9101511 9101512 910151E 9101515 150 mm 9101561 9101562 910156E 9101565 200 mm 9101521 9101522 910152E 9101525 250 mm 9101571 9101572 910157E 9101575

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 5%pH range: 2.5 to 8temperature limit: 80 °C

SiCH3 CH3

O

C1

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 9%end-cap: yespH range: 2.5 to 8temperature limit: 80 °C

SiCH3 CH3

O

C4

Search by compound name, synonym, CAS #, or keyword

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Chromatogram Search Tool

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LC COLUMNS

Columns for HPLC


Ultra Aromax is a unique reversed-phase material that exhibits extreme retention and selectivity for aromatic and/or unsaturated compounds. Th is column is a great alternative to our Biphenyl phase when increased retention is required, and it’s an excellent choice for gradient LC-MS analyses when conventional columns are not giving adequate retention or selectivity.

Ultra Aromax Columns (USP L11)



100 mm 9127311 9127312 912731E 9127315 150 mm 9127361 9127362 912736E 9127365


100 mm 9127511 9127512 912751E 9127515 150 mm 9127561 9127562 912756E 9127565 200 mm 9127521 9127522 912752E 9127525 250 mm 9127571 9127572 912757E 9127575

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 17%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L11phase category: phenylligand type: proprietary phenyl ligand

Aromax

SiCH3

Proprietary

CH3

O



Ultra Aqueous C18 Columns (USP L1)



100 mm 9178311 9178312 917831E 9178315 150 mm 9178361 9178362 917836E 9178365


100 mm 9178511 9178512 917851E 9178515 150 mm 9178561 9178562 917856E 9178565 200 mm 9178521 9178522 917852E 9178525 250 mm 9178571 9178572 917857E 9178575

Disp

ersion H-Bonding


ge


Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 15%end-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: modifi ed C18ligand type: proprietary polar modifi ed and functionally bonded C18

Aqueous C18

Si

OO

P

152 www.restek.com

LC COLUMNS

Columns for HPLC



Ultra Biphenyl Columns (USP L11)



100 mm 9109311 9109312 910931E 9109315 150 mm 9109361 9109362 910936E 9109365


100 mm 9109511 9109512 910951E 9109515 150 mm 9109561 9109562 910956E 9109565 200 mm 9109521 9109522 910952E 9109525 250 mm 9109571 9109572 910957E 9109575

Steroid Panel Analysis on the Ultra Biphenyl

1

2

3

15

16

4

5

6

7

8

9

10

11

12

13

4.3 4.4 4.5 4.6 4.7 4.8 4.9 5.0 5.1 5.2 5.3 5.4 5.5 5.6

0.50.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5

Time (min)

14

LC_CF0537

➲ See page 698 for conditions and peak list.

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 15%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase: L11phase category: phenylligand type: unique Biphenyl

Biphenyl

Disp

ersion H-Bonding


ge


SiCH3 CH3

O

www.restek.com 153

LC COLUMNS

Columns for HPLC


Th e Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases.

Ultra IBD Columns (USP L68)



100 mm 9175311 9175312 917531E 9175315 150 mm 9175361 9175362 917536E 9175365


100 mm 9175511 9175512 917551E 9175515 150 mm 9175561 9175562 917556E 9175565 200 mm 9175521 9175522 917552E 9175525 250 mm 9175571 9175572 917557E 9175575

Column Characteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 12%end-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L68phase category: polar-embedded alkylligand type: proprietary polar functional embedded alkyl

IBD

Disp

ersion H-Bonding


ge


CH3 CH3

O

Si

P



Ultra PFP Propyl Columns (USP L43)



100 mm 9179311 9179312 917931E 9179315 150 mm 9179361 9179362 917936E 9179365


100 mm 9179511 9179512 917951E 9179515 150 mm 9179561 9179562 917956E 9179565 200 mm 9179521 9179522 917952E 9179525 250 mm 9179571 9179572 917957E 9179575

Column Characteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 11%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L43phase category: fl uorophenyl propylligand type: pentafl uorophenyl propyl

PFP Propyl

Disp

ersion H-Bonding


ge


SiCH3 CH3

O

FF

FF

F

154 www.restek.com

LC COLUMNS

Columns for HPLC



Ultra Cyano Columns (USP L10)

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 8%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L10phase category: cyanoligand type: cyanopropylsilane

Cyano

SiCH3

NC

CH3

O


Th e general-purpose Restek Amino is an aminopropylsilane that off ers reproducible retention and effi ciency. It is a great choice for the normal-phase or HILIC analysis of simple sugars.

Ultra Amino Columns (USP L8)



100 mm 9107311 9107312 910731E 9107315 150 mm 9107361 9107362 910736E 9107365


100 mm 9107511 9107512 910751E 9107515 150 mm 9107561 9107562 910756E 9107565 200 mm 9107521 9107522 910752E 9107525 250 mm 9107571 9107572 910757E 9107575

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åcarbon load: 2%end-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L8phase category: aminoligand type: aminopropylsilane Amino

SiCH3

NH2

CH3

O



Ultra Silica Columns (USP L3)



100 mm 9100311 9100312 910031E 9100315 150 mm 9100361 9100362 910036E 9100365


100 mm 9100511 9100512 910051E 9100515 150 mm 9100561 9100562 910056E 9100565 200 mm 9100521 9100522 910052E 9100525 250 mm 9100571 9100572 910057E 9100575

Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 Åend-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L3phase category: bare silicaligand type: none Silica

Si

OO O

OH



100 mm 9106311 9106312 910631E 9106315 150 mm 9106361 9106362 910636E 9106365


100 mm 9106511 9106512 910651E 9106515 150 mm 9106561 9106562 910656E 9106565 200 mm 9106521 9106522 910652E 9106525 250 mm 9106571 9106572 910657E 9106575

www.restek.com 155

LC COLUMNS

Columns for Bioseparations

Viva HPLC Columns: 3 or 5 μm particles; 300 Å pore size • Excellent for separating peptides or proteins.• Rugged, spherical particles with 300 Å pore size.• High proportion of pore/surface area available to large molecules. Viva columns are based on a wide-pore material we designed for optimal large-molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean with a signifi cant portion of their pore volume falling below 150 Å. Th is means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.




Viva C18 Columns (USP L1)



100 mm 9514311 9514312 951431E 9514315 150 mm 9514361 9514362 951436E 9514365


100 mm 9514511 9514512 951451E 9514515 150 mm 9514561 9514562 951456E 9514565 200 mm 9514521 9514522 951452E 9514525 250 mm 9514571 9514572 951457E 9514575


Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 300 Åcarbon load: 9%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: C18, octadecylsilaneligand type: monomeric C18

C18

SiCH3 CH3

O






100 mm 9513511 9513512 951351E 9513515 150 mm 9513561 9513562 951356E 9513565 200 mm 9513521 9513522 951352E 9513525 250 mm 9513571 9513572 951357E 9513575

Column C haracteristics: particle size: 5 μm, sphericalpore size: 300 Åcarbon load: 5%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L7phase category: C8, octylsilaneligand type: monomeric C8

SiCH3 CH3

O

C8


156 www.restek.com

LC COLUMNS



Base-deactivated, wide-pore packing exhibits excellent peak shape for a wide range of compounds. Less retention in reversed-phase assays than Viva C18 or Viva C8.




100 mm 9512511 9512512 951251E 9512515 150 mm 9512561 9512562 951256E 9512565 200 mm 9512521 9512522 951252E 9512525 250 mm 9512571 9512572 951257E 9512575


Column C haracteristics: particle size: 5 μm, sphericalpore size: 300 Åcarbon load: 3.5%end-cap: yespH range: 2.5 to 8temperature limit: 80 °C

C4

SiCH3 CH3

O



Viva Biphenyl Columns (USP L11)



100 mm 9516511 9516512 951651E 9516515 150 mm 9516561 9516562 951656E 9516565 200 mm 9516521 9516522 951652E 9516525 250 mm 9516571 9516572 951657E 9516575


Column C haracteristics: particle size: 5 μm, sphericalpore size: 300 Åcarbon load: 7%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase: L11phase category: phenylligand type: unique Biphenyl

Biphenyl

SiCH3 CH3

O



Viva PFP Propyl Columns (USP L43)



100 mm 9519511 9519512 951951E 9519515 150 mm 9519561 9519562 951956E 9519565 200 mm 9519521 9519522 951952E 9519525 250 mm 9519571 9519572 951957E 9519575


Column C haracteristics: particle size: 5 μm, sphericalpore size: 300 Åcarbon load: 5%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L43phase category: fl uorophenyl propylligand type: pentafl uorophenyl propyl

PFP Propyl

SiCH3 CH3

O

FF

FF

F

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LC COLUMNS




Viva Silica Columns (USP L3)



100 mm 9510511 9510512 951051E 9510515 150 mm 9510561 9510562 951056E 9510565 200 mm 9510521 9510522 951052E 9510525 250 mm 9510571 9510572 951057E 9510575


Column C haracteristics: particle size: 5 μm, sphericalpore size: 300 Å pH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L3phase category: bare silicaligand type: none

Silica

Si

OO O

OH

25270

• Significant savings over instrument manufacturer prices.

• High-quality components in every kit.

• Wide range of options for LC systems and pumps.

Choose Cost-Effective Restek LC KitsFor Your Preventative Maintenance

25145

www.restek.com/LC-Maintenance

See page 299 for Agilent-system kits or page 307 for Waters-system kits.

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LC COLUMNS

Application-Specific Columns

Column C haracteristics: particle size: 4 μm, spherical pore size: 110 Å end-cap: no pH range: 2.5 to 8temperature limit: 80 °C

Application-Specifi c LC Phases


Developed specifi cally for challenging analyses of polycyclic aromatic hydrocarbons (PAHs). Th e Pinnacle® II PAH stationary phase incorporates a proprietary C18 bonding that enables unique shape selectivity to baseline resolve all 16 PAHs listed in U.S. EPA Method 610 plus two other routinely analyzed PAH compounds. Every lot of Pinnacle® II PAH bonded phase material is tested to ensure baseline resolution of the Method 610 PAHs using a simple water/acetonitrile mobile phase gradient. Further, because we make Pinnacle® II PAH columns using our own silica, we have greater control over quality and reproducibility. If you are analyzing PAHs using HPLC, Pinnacle® II PAH columns are a reliable, cost-eff ective choice.

Pinnacle® II PAH HPLC Columns

Pinnacle® II PAH Guard Cartridges

2.1 mm ID 3.0 mm ID 4.6 mm IDLength cat.# cat.# cat.#

4 μm Columns 50 mm 9219452 921945E 9219455

100 mm 9219412 921941E 9219415 150 mm 9219462 921946E 9219465 200 mm 9219422 921942E 9219425 250 mm 9219472 921947E 9219475

Guard Cartridges 3-pk.(10 x 2.1 mm)

3-pk.(10 x 4.0 mm)

Pinnacle II PAH Guard Cartridge 921950212 921950210

customer service: 1-814-353-1300, ext. 3 | e-mail: [email protected]

technical service: 1-814-353-1300, ext. 4 | e-mail: [email protected]

sales representatives: 1-814-353-1300, ext. 3 | e-mail: [email protected]

4 ways to order:1. CALL: 1-800-356-1688, ext. 3 or 1-814-353-1300, ext. 3 Monday–Friday 8:00 a.m.–6:30 p.m. Eastern Time

2. FAX: 1-814-353-1309 24 hours a day

3. ONLINE: www.restek.com 24 hours a day

4. E-MAIL: [email protected] 24 hours a day

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LC COLUMNS



Specifi cally designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Get complete resolution of all 16 EPA 610 PAHs plus two other routinely analyzed PAH com-pounds in less than fi ve minutes to greatly reduce run times and increase sample throughput.

Pinnacle® DB PAH UHPLC Columns


Column C haracteristics: particle size: 1.9 μm, sphericalpore size: 140 Å end-cap: no pH range: 2.5 to 8temperature limit: 80 °C

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00Time (min)

1 2 3 4 5

6

7

8

9 10

11

12

13

1415

1617

18

PAH Mix on Pinnacle® DB PAH

LC_EV0521

Peaks 1. Naphthalene 2. Acenaphthylene 3. 1-Methylnaphthalene 4. 2-Methylnaphthalene 5. Acenaphthene 6. Fluorene 7. Phenanthrene 8. Anthracene 9. Fluoranthene 10. Pyrene 11. Benzo[a]anthracene 12. Chrysene 13. Benzo[b]fl uoranthene 14. Benzo[k]fl uoranthene 15. Benzo[a]pyrene 16. Dibenzo[a,h]anthracene 17. Benzo[ghi]perylene 18. Indeno[1,2,3-cd]pyrene

Column Pinnacle® DB PAH (cat.# 9470252)Dimensions: 50 mm x 2.1 mm IDParticle Size: 1.9 μmPore Size: 140 ÅTemp.: 30 °CSample EPA Method 8310 PAH Mixture (cat.# 31841)Diluent: acetonitrileConc.: 10 μg/mLInj. Vol.: 1 μLMobile Phase A: waterB: acetonitrile Time (min) Flow (mL/min) %A %B 0 0.8 60 40 2 0.8 40 60 4 0.8 0 100 4.5 0.8 0 100 4.51 0.8 60 40 5 0.8 60 40Max Pressure: 724 barDetector Photo diode array @ 254, 4.8 nmInstrument Waters


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Carbonyls by CARB Method 1004 on Allure® AK

LC COLUMNS


Column C haracteristics: particle size: 5 μm, sphericalpore size: 60 Åend-cap: yespH range: 2.5 to 8temperature limit: 80 °C

Column Allure® AK (cat.# 9159525-700)Dimensions: 200 mm x 4.6 mm IDParticle Size: 5 μmPore Size: 60 ÅTemp.: 30 °CSample dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketonesDiluent: acetonitrileConc.: 3 μg/mL each analyte, as aldehyde/ketoneInj. Vol.: 10 μLMobile Phase A: waterB: acetonitrile

Time (min) %B 0 60 8 70 10 100Flow: 1.5 mL/minDetector UV/Vis @ 360 nm

1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 Time (min)

1

2

3

4 5

67

89

10

11 12

13

LC_EV0393

Peaks RT (min) 1. Formaldehyde 4.74 2. Acetaldehyde 5.78 3. Acrolein 6.86 4. Acetone 7.09 5. Propionaldehyde 7.31 6. Crotonaldehyde 8.19 7. Methacrolein 8.55 8. Butyraldehyde 8.79 9. Methyl ethyl ketone 9.06 10. Benzaldehyde 10.03 11. Valeraldehyde 10.39 12. m-Tolualdehyde 11.08 13. hexanaldehyde 11.36DNPH derivatives of aldehydes and ketones


Th is highly retentive, highly selective phase—unique to Restek—was developed specifi cally for the analysis of aldehydes and ketones as DNPH derivatives. Allure® AK is a reversed-phase HPLC material that has the unique ability to separate all 13 carbonyl compounds specifi ed in California Air Resources Board (CARB) Method #1004, using a simple acetonitrile/water gradient. Other columns require long analysis times or the use of tetrahydrofuran.

Allure® AK Columns

Allure® AK Guard Cartridge

3.2 mm ID 4.6 mm IDLength cat.# cat.#

5 μm Columns with Trident Integral Inlet Fitt ings 200 mm 9159523-700 9159525-700


Allure AK Guard Cartridge 915950210

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Organic Acids on Allure® Organic Acids

LC COLUMNS


Column C haracteristics: particle size: 5 μm, sphericalpore size: 60 Åend-cap: nopH range: 2.5 to 8temperature limit: 80 °C

0 5

1

23

4

5

10 15 20 25 30 35 40 Time (min)

LC_0238

Column Allure® Organic Acids (cat.# 9165585)Dimensions: 300 mm x 4.6 mm IDParticle Size: 5 μmPore Size: 60 ÅTemp.: ambientSample standard solutionDiluent: waterInj. Vol.: 10.0 μLMobile Phase 100 mM phosphate buff er, pH 2.5Flow: 0.5 mL/minDetector UV/Vis @ 226 nm

Peaks Conc. (mg/mL) 1. Tartaric acid 1.0 2. Quinic acid 1.0 3. Malic acid 1.0 4. Citric Acid 1.0 5. Fumaric Acid 0.005


Allure® Organic Acids columns provide enhanced retention and selectivity for polar organic acids, allowing the separation to be performed on a single 30 cm column. An Allure® Organic Acids column eff ectively resolves key organic acids such as tartaric and quinic acids using the chromatographic conditions specifi ed in AOAC method 986.13. Retention is stable and reproducible, even with the 100% aqueous mobile phase specifi ed in the AOAC method.

Allure® Organic Acids Columns

Allure® Organic Acids Guard Cartridges


5 μm Column 150 mm 916556E 9165565 250 mm 9165575 300 mm 9165585


3-pk.(10 x 4.0 mm)

Allure Organic Acids Guard Cartridge 916550212 916550210

162 www.restek.com

Carbamates on Ultra Carbamate

LC COLUMNS


Column C haracteristics: particle size: 3 μm or 5 μm, sphericalpore size: 100 ÅpH range: 2.5 to 8temperature limit: 80 °C

Column Ultra Carbamate (cat.# 9177352)Dimensions: 50 mm x 2.1 mm IDParticle Size: 3 μmPore Size: 100 ÅTemp.: ambientSample 531.1 Carbamate Pesticide Calibration Mixture (cat.# 32273) 4-bromo-3,5-dimethylphenyl-N-methylcarbamate (BDMC)

(cat.# 32274)Diluent: methanolConc.: 50 μg/mLInj. Vol.: 1 μLMobile Phase A: 2 mM ammonium acetate:methanol (v/v, 90/10)B: 2 mM ammonium acetate:methanol (v/v, 10/90) Time (min) %A %B 0.00 80 20 20 0 100 25 0 100Flow: 0.2 mL/minDetector LECO Unique® TOFMSRun Length: 25 minIonization Source Type: high fl ow ESIIon Mode: positiveDesolvation Temp.: 130 °CNebulizing Pressure: 100 kPaDesolvation Gas (N2): 4 L/minInterface Temp.: 120 °CNozzle Voltage: 62 VCapillary Voltage: 2.75 kVInstrument Agilent 1100Acknowledgement LECO Corporation

0 2.5

12

34

56

7

8

9

10

11

5.0 7.5 10.0 12.5 15.0 17.5 20.0 Time (min) LC_FF0473

Peaks 1. Aldicarb sulfone 2. Aldicarb sulfoxide 3. Oxamyl 4. Methomyl 5. 3-Hydroxycarbofuran 6. Aldicarb 7. Propoxur 8. Carbofurane 9. Carbaryl 10. Methiocarb 11. BDMC (IS)


Restek chemists developed the Ultra Carbamate column specifi cally for carbamates analysis. Th e unique packing separates 10 target carbamates in just over 10 minutes. Th e column is compatible with fl uorescence or LC-MS detection.* An Ultra Carbamate column can process as many as three samples per hour, versus less than two samples per hour on a general-purpose C18 column. In addition to increased sample throughput, this much faster analysis will signifi cantly reduce solvent usage—and the costs of disposing of solvent waste.

Ultra Carbamate Columns

*For post-column derivatization/fl uorescence detection applications using a 4.6 mm ID column, the total system dead volume, including the post-column reactor, must be less than 650 μL. For standard post-column reactor systems, we recommend a 250 mm x 4.6 mm, 5 μm column. Contact Restek Technical Service or your local Restek representative for more information.

Ultra Carbamate Guard Cartridges


3 μm Columns 50 mm 9177351 9177352 917735E 9177354 9177355

100 mm 9177311 9177312 917731E 9177315 5 μm Columns

250 mm 9177575


3-pk.(10 x 4.0 mm)

Ultra Carbamate Guard Cartridge 917750212 917750210

www.restek.com 163

Paraquat and Diquat on Ultra Quat

LC COLUMNS



A retentive, high-purity, base-deactivated, reversed-phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines. Column Characteristics:

particle size: 3 μm or 5 μm, sphericalpore size: 100 ÅpH range: 2.5 to 8temperature limit: 80 °C

Ultra Quat Columns

Ultra Quat Guard Cartridges


3 μm Column 50 mm 9181352

5 μm Column 150 mm 9181565


3-pk.(10 x 4.0 mm)

Ultra Quat Guard Cartridge 918150212 918150210

LC_EV0383 Column Ultra Quat (cat.# 9181352)Dimensions: 50 mm x 2.1 mm IDParticle Size: 3 μmPore Size: 100 ÅTemp.: ambientSampleDiluent: DI WaterConc.: 5 μg/mL each componentInj. Vol.: 10 μLMobile Phase 10 mM heptafl uorobutyric acid:acetonitrile (95:5)Flow: 0.3 mL/minDetector Applied Biosystems/MDS Sciex LC-MS/MSModel #: API 3200™ MS/MS systemIon Source: ElectrosprayIon Mode: ESI+Ion Spray Voltage: 5.5 kVCurtain Gas: 15 psi (103.4 kPa)Gas 1: 70 psi (482.6 kPa)Gas 2: 60 psi (413.7 kPa)Source Temp.: 600 °CMode: MRMDwell Time: 200 msInstrument Applied Biosystems/MDS Sciex LC-MS/MS SystemNotes Collision exit potential: 3V Q1/Q3: unit resolutionAcknowledgement Data courtesy of Houssain El Aribi, Ph.D., LC/MS Product and Application Specialist,

MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8

Peaks Precursor Ion Fragment Ion DP Collision Energy (amu) (amu) (V) (eV) 1. Diquat 183+ 157+ 30 30 2. Paraquat 93 (2+) 171+ 20 20

Fast, sensitive

LC-MS/MS analysis!

164 www.restek.com

LC COLUMNS

Prep Columns

Ultra Preparative ColumnsUsing Restek Ultra columns for preparative applications can save you time, solvents, and money. By utilizing the right phase for your prep analysis, you can make sure your peaks are resolved and your compounds are pure. Th e Ultra line has high loading and features high-purity silica.



Ultra Aqueous C18 HPLC Prep Columns

USLC® Phases for Preparative HPLC

Column Characteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 15%end-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: modifi ed C18ligand type: proprietary polar modifi ed and functionally bonded C18

Dis

pers

ion H-Bondin

g

Po

larizability Cation E

xcha

ng

e


Aqueous C18

Si

OO

P


Th e Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very reten-tive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases.

Ultra IBD HPLC Prep Columns Column Characteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 12%end-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L68phase category: polar-embedded alkylligand type: proprietary polar functional embedded alkyl

IBD

Dis

pers

ion H-Bondin

g

Po


xcha

ng

e


CH3 CH3

O

Si

P

10 mm ID 21.2 mm ID 30 mm IDLength cat.# cat.# cat.#

5 μm Columns 50 mm 9178557 9178558 9178559

100 mm 9178517 9178518 9178519 150 mm 9178567 9178568 9178569 250 mm 9178577 9178578 9178579


5 μm Columns 50 mm 9175557 9175558 9175559

100 mm 9175517 9175518 9175519 150 mm 9175567 9175568 9175569 250 mm 9175577 9175578 9175579

www.restek.com 165

LC COLUMNS

Prep Columns



Ultra Biphenyl Prep Columns

PLEASE NOTE: We strongly recommend ordering a semi-prep or prep column only after evaluating the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns.

Before You Buy a Prep Column...

Column Characteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 15%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase: L11phase category: phenylligand type: unique Biphenyl

Dis

pers

ion H-Bondin

g

Po


xcha

ng

e


Biphenyl

SiCH3 CH3

O



Ultra PFP Propyl Prep Columns Column Characteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 11%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L43phase category: fl uorophenyl propylligand type: pentafl uorophenyl propyl

Dis

pers

ion H-Bondin

g

Po


xcha

ng

e


PFP Propyl

SiCH3 CH3

O

FF

FF

F


5 μ m Co lumns 50 mm 9109557 9109558 9109559

100 mm 9109517 9109518 9109519 150 mm 9109567 9109568 9109569 250 mm 9109577 9109578 9109579


5 μm Columns 50 mm 9179557 9179558 9179559

100 mm 9179517 9179518 9179519 150 mm 9179567 9179568 9179569 250 mm 9179577 9179578 9179579

166 www.restek.com

LC COLUMNS

Prep Columns



Column C haracteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 20%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L1phase category: C18, octadecylsilaneligand type: monomeric C18

Ultra C18 HPLC Prep Columns

Traditional Phases for Preparative HPLC

C18

Column C haracteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 12%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L7phase category: C8, octylsilaneligand type: monomeric C8

SiCH3 CH3

O

C8



Ultra C8 HPLC Prep Columns

SiCH3 CH3

O

PLEASE NOTE: We strongly recommend ordering a semi-prep or prep column only after evaluating the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns.

Before You Buy a Prep Column...


5 μm Columns 50 mm 9174557 9174558 9174559

100 mm 9174517 9174518 9174519 150 mm 9174567 9174568 9174569 250 mm 9174577 9174578 9174579


5 μm Columns 50 mm 9103557 9103558 9103559

100 mm 9103517 9103518 9103519 150 mm 9103567 9103568 9103569 250 mm 9103577 9103578 9103579

www.restek.com 167

LC COLUMNS

Prep Columns, Bulk Packing Materials

Column C haracteristics: particle size: 5 μm, sphericalpore size: 100 Åend-cap: nopH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L3phase category: bare silicaligand type: none

Silica

Si

OO O

OH



Cyano

SiCH3

NC

CH3

O

Column C haracteristics: particle size: 5 μm, sphericalpore size: 100 Åcarbon load: 8%end-cap: yespH range: 2.5 to 8temperature limit: 80 °CUSP phase code: L10phase category: cyanoligand type: cyanopropylsilane

Ultra Cyano HPLC Prep Columns



Ultra Silica HPLC Prep Columns

Ultra Bulk Packing Materials (5 μm) Use our bulk packing materials to pack your own columns! • Prepare your own columns in conventional or custom dimensions.• Consistent, high-quality materials. Our extensive QC program ensures the high quality and reproducibility of these sili-cas. You can be confi dent that you are getting consistent, high-quality product when you source your silica from Restek.

Use these materials for easy scale-up to preparative chromatography or for packing your own columns.

also availableOther stationary phases and particle sizes are also available in bulk; call Customer Service or your local Restek representative for details.

Description qty. cat.# 1-9 bott les 10-49 bott les 50-99 bott les ≥100 bott les5 μm Ultra Bulk Packing Materials Ultra C1 Bulk Packing 10 g/btl. 91015 $398/btl. $373/btl. $352/btl. Ultra C4 Bulk Packing 10 g/btl. 91025 $398/btl. $373/btl. $352/btl. Ultra C8 Bulk Packing 10 g/btl. 91035 $398/btl. $373/btl. $352/btl. Ultra C18 Bulk Packing 10 g/btl. 91745 $398/btl. $373/btl. $352/btl. Ultra Amino Bulk Packing 10 g/btl. 91075 $398/btl. $373/btl. $352/btl. Ultra Cyano Bulk Packing 10 g/btl. 91065 $398/btl. $373/btl. $352/btl. Ultra Silica Bulk Packing 10 g/btl. 91005 $398/btl. $373/btl. $352/btl.


5 μm Columns 50 mm 9106557 9106558 9106559

100 mm 9106517 9106518 9106519 150 mm 9106567 9106568 9106569 250 mm 9106577 9106578 9106579


5 μm Columns 50 mm 9100557 9100558 9100559

100 mm 9100517 9100518 9100519 150 mm 9100567 9100568 9100569 250 mm 9100577 9100578 9100579

168 www.restek.com

LC COLUMNS

UHPLC Filters

UltraLine UHPLC In-Line Filter • In-line design installs easily with standard fi ttings. • Cost-eff ective protection for UHPLC systems. • Reliable way to extend column lifetime. • Leak-tight to 15,000 psi (1,034 bar). • Replaceable 0.5 μm stainless steel fi lter in stainless

steel body. Specifi cations: Inlet/Outlet: Female/Female 10-32Port Geometry: Parker (1/16 CPI)Material: Stainless steel housingFilter: 0.5 μm stainless steel, 0.125" W x 0.062" T, 5 μL volumePressure Rating: 15,000 psig (1,034 bar)Wrench Flat: 3/8"

Description qty. cat.# UltraLine UHPLC In-Line Filter (In-Line Assembly with Filter) ea. 24993

UltraLine Replacement Filters 5-pk. 24994

UltraShield UHPLC PreColumn Filter • Cost-eff ective protection for UHPLC systems. • Reliable way to extend column lifetime. • Universal fi t—connects easily to any brand column. • Leak-tight to 15,000 psi (1,034 bar). • 0.5 μm titanium fi lter in stainless steel body with

PEEK ferrule. Specifi cations:Inlet/Outlet: Female/Male 10-32Port Geometry: Parker (1/16 CPI)Material: Stainless steel, PEEK ferruleFilter: 0.5 μm titaniumPressure Rating: 15,000 psig (1,034 bar)Wrench Flat: 5/16"

Description qty. cat.# UltraShield UHPLC PreColumn Filter ea. 24995 UltraShield UHPLC PreColumn Filter 5-pk. 24996 UltraShield UHPLC PreColumn Filter 10-pk. 24997

24995

24993

Protect Your Column and Your UHPLC Performance With UltraShield and UltraLine UHPLC FiltersA cost-effective way to extend the lifetime of

any UHPLC column without sacrificing your

UHPLC performance on any LC system

Use with any

UHPLC system

www.restek.com 169

LC COLUMNS

HPLC Guard Cartridge Systems

25022

Double the Protection With Cap Frits!

XF Fitting

XG-XF Fitting

GuardCartridge

PEEKTip

CapFrit

*The standard PEEK tip in Trident direct systems is compatible with Parker, Upchurch Scientifi c, Valco, and other CPI-style fi tt ings. To use Trident direct systems with Waters-style end fi tt ings, replace the tip with cat.# 25088.

Trident Direct Guard Cartridge System Easy to Use, Low Dead Volume—The Ultimate Combination of Convenience and Column Protection

Unlike “one size fi ts all” guard systems, the Trident direct system gives you the power to select the right level of protection for your analysis. The system offers three levels of protection and guard cartridges in four dimensions, with a variety of bonded phases to match your analytical column. The economical, leak-free cartridge design provides an unprecedented combination of convenience, economy, and reliability. The founda-tion of the Trident direct system is a reusable direct connect holder that easily attaches to any HPLC column using CPI- or Waters-style end fi ttings.* The system is available in confi gurations to match different protection levels: in-line fi lter, in-line fi lter with holder for 10 mm guard cartridge. The guard cartridges are available in 2.1 and 4.0 mm ID and are interchangeable within the appropriate length holder.

25082 Protection against particulate matter.

25084

Protection against particulate matter and moderate protection against irreversibly

adsorbed compounds.

Description qty. cat.# High-pressure fi lter ea. 25082 10 mm guard cartridge holder without fi lter ea. 25083 10 mm guard cartridge holder with fi lter ea. 25084 PEEK tip for Waters-style end fi tt ings ea. 25088 PEEK tip for standard fi tt ings ea. 25087

Replacement Cap Frits

Replacement guard cartridges can cost as much as an analytical column, so why not protect them, too? The removable cap frit in a Trident direct helps prevent clogged cartridges to extend the life of your column, your cartridge, and your budget!

Description ID Porosity qty. cat.# Replacement Cap Frits 4 mm 2.0 μm 5-pk. 25022 Replacement Cap Frits 4 mm 0.5 μm 5-pk. 25023 Replacement Cap Frits 2 mm 2.0 μm 5-pk. 25057 Replacement Cap Frits 2 mm 0.5 μm 5-pk. 25990

Trident direct 10 mm guard cartridge holder with filter

Components

Assembled

Installed onto column

170 www.restek.com

Trident in-line 10 mm guard cartridge holder with filter

XF Fitting

XG-XF Fitting

GuardCartridge

Body

CapFrit

Components

Assembled

Installed onto column

LC COLUMNS

HPLC Guard Cartridge Systems

25057

Double the Protection With Cap Frits!

Replacement Cap Frits

Replacement guard cartridges can cost as much as an analytical column, so why not protect them, too? The removable cap frit in a Trident direct helps prevent clogged cartridges to extend the life of your column, your cartridge, and your budget!

Description ID Porosity qty. cat.# Replacement Cap Frits 4 mm 2.0 μm 5-pk. 25022 Replacement Cap Frits 4 mm 0.5 μm 5-pk. 25023 Replacement Cap Frits 2 mm 2.0 μm 5-pk. 25057 Replacement Cap Frits 2 mm 0.5 μm 5-pk. 25990

Trident HPLC In-Line Guard Cartridge Holders

A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting it with a short piece of 1/16" tubing, appropriate nuts and ferrules, or fi nger-tight fi ttings. The system can be used with Restek columns, or with columns from other manufacturers. Holders are available for either 10 mm or 20 mm guard car-tridges. Either size can be purchased with or without a prefi lter, which provides added protection against the particles that can shorten the lifetime of the guard cartridge.

25040 25021

Description qty. cat.# Holder for 10 mm guard cartridge ea. 25021 Holder with fi lter for 10 mm guard cartridge ea. 25040 Frit-Type In-Line Filter, 2.0 μm ea. 25041

Hybrid Ferrule U.S. Patent No. 8201854, Optimize Technologies. Optimize Technologies EXP Holders are Patent Pending. Other U.S. and Foreign Patents Pending. The Opti- prefi x is a registered trademark of Optimize Technologies, Inc.

EXP® Reusable Fittings for HPLC & UHPLC for 10-32 fi ttings and 1/16" tubing EXP® Hand-Tight Coupler

Description qty. cat.# EXP Hand-Tight Coupler (2 Nuts, 2 Ferrules, 1/16" x 0.005" ID Tubing) ea. 25940

25940

NEW!

For HPLC tubing, visit www.restek.com/lcacc

also available

Our entire EXP® fi tting selection.

See page 313.

www.restek.com 171

LC COLUMNS

HPLC Guard Cartridges

Trident HPLC Guard Cartridges

Guard Cartridge

Choose Columns Fast. Develop Methods Faster.

RESTEK USLC®Ultra Selective Liquid Chromatography

www.restek.com/uslc

See pages 137–139.

Description3-pk.

(10 x 2.1 mm)3-pk.

(10 x 4.0 mm)Pinnacle DB Guard Cartridges Pinnacle DB C18 Guard Cartridge 941450212 941450210 Pinnacle DB C8 Guard Cartridge 941350212 941350210 Pinnacle DB Aqueous C18 Guard Cartridge 941850212 941850210 Pinnacle DB Biphenyl Guard Cartridge 940950212 940950210 Pinnacle DB PFP Propyl Guard Cartridge 941950212 941950210 Pinnacle DB Cyano Guard Cartridge 941650212 941650210 Pinnacle DB Silica Guard Cartridge 941050212 941050210 Ultra Guard Cartridges Ultra C18 Guard Cartridge 917450212 917450210 Ultra C8 Guard Cartridge 910350212 910350210 Ultra C4 Guard Cartridge 910250212 910250210 Ultra C1 Guard Cartridge 910150212 910150210 Ultra Aromax Guard Cartridge 912750212 912750210 Ultra Aqueous C18 Guard Cartridge 917850212 917850210 Ultra Biphenyl Guard Cartridge 910950212 910950210 Ultra IBD Guard Cartridge 917550212 917550210 Ultra PFP Guard Cartridge 917650212 917650210 Ultra Cyano Guard Cartridge 910650212 910650210 Ultra Amino Guard Cartridge 910750212 910750210 Ultra Silica Guard Cartridge 910050212 910050210 Viva Guard Cartridges Viva C18 Guard Cartridge 951450212 951450210 Viva C8 Guard Cartridge 951350212 951350210 Viva C4 Guard Cartridge 951250212 951250210 Viva Biphenyl Guard Cartridge 951650212 951650210 Viva PFP Propyl Guard Cartridge 951950212 951950210 Viva Silica Guard Cartridge 951050212 951050210

lc columns - satia · lc columns uslc® columns evaluating and extending selectivity the restek...

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