lab safety - site · lab rules: • know the safety rules of the lab. • do not use equipment...

77
LAB SAFETY Forest Biomaterials North Carolina State University

Upload: others

Post on 28-May-2020

9 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

LAB SAFETY

Forest Biomaterials

North Carolina State University

Page 2: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

2

Page 3: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Lab Rules:

•Know the safety rules of the lab.

•Do not use equipment without training.

•Clean up!

•Ask permission to borrow anything.

• If you break something, let someone know.

Developed by Drs. Jameel, Lucia, and Saloni

(September 2008)3

Page 4: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

4

Emergency Numbers

OFFICE HOME

Campus Emergencies (Police, Fire, Spills) 911

(Identify as being at

NCSU)

Fire Protection 515-5127

Hazardous Material Division 515-6863 (6864)

Health & Safety Services 515-6858

Rex Hospital Emergency Room 784-3100

Wake Medical Center Emergency Room 350-8000

Carolinas Poison Center 1-800-848-6946

Barbara White, Lab Manager/CNR Safety 513-0298 753-8523

Vacant, Pilot Plant 515-7761

Tony LaPasha, Hodges Wood Products 515-2850

Page 5: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Common Hazards

Forest Biomaterials

5

Page 6: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Chemicals

6

Page 7: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Equipment

7

Page 8: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Compressed Gases

8

Page 9: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Magnet/Quench

9

Page 10: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Ultraviolet Lamp

10

Page 11: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

High Pressure

11

Page 12: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

High Voltage

12

Page 13: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Radiation

13

Page 14: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Nip/Pinch Point

14

Page 15: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Steam Lines

15

Page 16: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Noise

16

Page 17: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Slip Hazard/Wet Floor

17

Page 18: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Cuts

18

Page 19: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Sawdust

19

Page 20: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Protect yourself!

20

Know what hazards are present in your work area and how to

safeguard against them.

Page 21: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

21

Safety Plan

Location

Page 22: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Review MSDS for chemicals

22

MSDS available at

www3.ncmsds.com

or supplier.

Page 23: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Personal Protective Equipment

(PPE)

Select and wear the proper

personal protective equipment

to protect against the hazards

found in the safety plan and the

MSDS.

23

Page 24: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Safety Glasses

(All labs and shops)

24

Page 25: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Lab Coats

(All labs—not in shops)

25

Page 26: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Gloves

According to Hazard

26

Page 27: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Proper Shoes for Labs

27

Page 28: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Improper Shoes

NOT ACCEPTABLE!!!!

.

28

Page 29: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Suitable Clothing for Labs

29

Page 30: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

NOT PROPER ATTIRE

30

Page 31: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Foot Protection-

Pilot Plant Area

Steel-toes only!

31

Page 32: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Specialty PPE

32

Page 33: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Long Hair

33

Page 34: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

SAFETY EQUIPMENT

34

Page 35: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

35

Fire Extinguishers

Page 36: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Fire Extinguisher

DO NOT USE A FIRE EXTINGUISHER

UNLESS YOU HAVE BEEN CERTIFIED BY

FIRE SAFETY PERSONNEL.

36

Page 37: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

First Aid Kits

37

Page 38: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Eyewash and Safety Shower

38

Page 39: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Use of Eyewash

• Remove contact lenses if possible.

• Forcibly hold eyes open in eyewash.

• Have someone else turn on eyewash and call emergency response.

• Irrigation should last AT LEAST 15 MINUTES, even if emergency crews arrive more quickly.

39

Page 40: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

40

Safety Showers

Used in the event that significant amounts of

caustic, toxic, or otherwise hazardous

materials are spilled on yourself or others.

1. Get under the shower

2. Pull the handle down

3. If necessary, remove contaminated clothing or shoes

Generally located in the hallways outside of

laboratories.

Page 41: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

E Stop

41

Page 42: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

42

E-Stops

• Typically located near the door.

• Shut off power to electrical wall outlets

Useful in fire fighting or electrocution

• Do not cut power to the lighting

• Do not cut power to high voltage equipment

Page 43: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

SAFETY

ALARMS

•Fire Alarm

•Oxygen Sensor (NMR Room)

•Walk-in Refrigerators

•Fume Hood

43

Page 44: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

DO NOT WORK ALONE!

At least one other authorized person must

be present in the general work area.

Friends, wives, children or other unauthorized

students must not be in the laboratory.

44

Page 45: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Security Measures

•No visitors or tours of labs without

permission of PI or responsible staff.

•Lab doors should be locked when

unoccupied.

•Outside doors should be locked after hours.

•Report suspicious people to Campus Police

and department head.

45

Page 46: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

NO FOOD OR DRINK!!!

46

Including empty wrappers and cups!!! This is an OSHA

violation!

Page 47: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Labeling of Materials

•Chemicals and stock solutions

•Experiments

•Hazardous wastes

47

Page 48: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Proper Labeling of Chemicals

and Stock Solutions

•Name(s) and concentration(s) of

components

•Date of preparation

•Your name

•PI name

•Hazard i.d. (HMIS)

48

Page 49: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Labeling of Stock Solutions

49

Page 50: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

50

Hazardous Materials Identification

System (HMIS)

Health (BLUE)

4 Deadly

3 Extreme danger

2 Hazardous

1 Slight Hazard

0 Normal

FIRE (RED)

Flash Points

4 Below 73 F

3 Below 100 F

2 Below 200 F

1 Above 200 F

0 Will not burn

REACTIVITY (YELLOW)

4 May detonate

3 Shock and heat

2 Violent chemical reactions

1 Unstable if heated

0 Stable

SPECIFIC HAZARDS

(WHITE)

Oxidizer OX

Acid ACID

Alkali ALK

Corrosive COR

Use NO Water W

Radiation Hazard

Bio Hazard

0 – 1 REASONABLY SAFE

2 SOME HAZARD – USE CAUTION

3 – 4 HAZARDOUS, BE VERY CAUTIOUS

Page 51: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Proper Labeling of Experiment

• ―Experiment in Progress‖

•Your name

•PI name

•General description of matrix/hazard

•Key to sample IDs referenced

51

Page 52: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Experiment Labeling-Good Start!

52

Page 53: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Hazardous Waste Labeling

•HAZARDOUS WASTE

•Components (with concentrations or

volume)

•PI’s Name (or room number of lab)

•Start date of collection

53

Page 54: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

54

Fire Prevention Guidelines

Page 55: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Fire Safety Tips

•Locate nearest fire alarm box

•Clear exits

•Store flammable/combustible materials in

appropriate containers away from heat and

spark sources.

•Never leave open flame unattended.

•Regularly check electrical cords/switches.

•No extension cords.

55

Page 56: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

In the Event of a Fire

• Activate pull station.

• If there is time, turn off open flames, turn off

instrumentation, and stabilize experiments.

• Pull down fume hood sashes.

• Close the door.

• Alert others.

• Exit the building—do not use elevators!!

• Call 911 from another building or from a cell phone

outside. Identify as being at NC State.

• Assemble at meeting area and account for everyone

• Do not re-enter the building until cleared by first

responders.56

Page 57: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Surviving a Building Fire

• Never use an elevator.

• Check closed doors before opening; if hot, choose another

route.

• Crawl if there is smoke.

• Take short breaths, freshest air is near the door.

• If you cannot leave—stuff towel, clothing, etc., under door,

over vents to restrict smoke and fumes.

• Open window if possible.

• Call 911 and give your exact location.

• Use a towel, sheet, pillowcase, or clothing to hang out the

window to mark your location for first responders to see.

57

Page 58: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Safe Handling of Chemicals

•Proper labeling of all materials.

•Maintain current inventory

•No 20 L cans without permission of lab

manager

•Minimize exposure to all chemicals

•No eating, drinking, or applying cosmetics

in labs.

•Avoid mouth pipetting and inhalation.

58

Page 59: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Safe Handling of Chemicals

(Cont.)

•Clean up residue from counters, containers,

and equipment daily!

•Transport chemicals in bottle carriers.

•Wash your hands when leaving the lab.

59

Page 60: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Chemical Storage

•Large quantities of acids, alkalis, or

solvents should not be in the department.

•Order the least amount of chemicals that are

needed to complete the task.

• Store in proper cabinet(s).

•No chemicals stored overhead.

•Secondary containment

60

Page 61: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Example of Secondary

Containment

61

Page 62: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Major

Hazardous Waste Rules

• Hazardous wastes and stock chemicals MUST be

stored separately.

• NO hazardous waste may be carried through a

door or into a hallway.

• Do not stockpile hazardous waste—submit for

pick-up as soon as the bottle is 2/3 full.

• Separate incompatible chemicals.

• Use suitable container and secondary containment.

62

Page 63: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Chemical Disposal

Following information required:

• Components (with concentrations)

• State (solid, liquid, gas)

• Volume or weight (include units)

• Location

Contact person for submission:

3rd Floor: Barbara White

1st and Second Floors: Linda McMurray

Wood Products: Tony LaPasha

63

Page 64: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Label for Waste Pick-up

HAZARDOUS WASTE DATE_________________

State: Solid / liquid / gas Wt. or Vol.:

Form Number: ________________________________________

Generator: ____________________________________________

Building: _____________________________________________

Corrosive _____ Flammable _____ Air/H2O Reactive______

Constituents___________________________________________

_______________________________________________________________________________________________

_____________

64

Page 65: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Chemical Spills

•When in doubt, evacuate the area and notify

lab manager or PI. After hours, call 911.

•Small spills---use Chemsorb to absorb and

dispose as hazardous waste.

•Large spills (especially of fuming acids)—

evacuate and call 911.

65

Page 66: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Wolf Alert Emergency

Communications

• Text Messaging

• Home Page Messaging www.ncsu.edu

• Broadcast Email

• Audible Alert

• Emergency Listserv

• Campus Hotline—513-8888

• Media

• Crime Alerts

Access:

http://www.ncsu.edu/ehs/campus_alert.htm66

Page 67: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Lab Operations

67

Page 69: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Training for Equipment

•Pilot Plant—Vacant

•Pulping/Bleaching—Linda McMurray

•Hodges Wood Products Lab—Tony

LaPasha

69

Page 70: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Analytical Facilities

•See Barbara White to locate responsible

user.

•Make appointment for training.

•Set up account for instrument reservation.

•Reserve time online:

http://www.cgibin.ncsu.edu/wps-

bin/calendar_admin.pl

70

Page 71: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Equipment Requiring

Reservations (No Use Rate)

•Autoclave

•High speed centrifuge

• Incubator (Biotech Lab)

•DCA

•UV-Vis spectrophotometers

•SPRs

•QCMs

•Muffle Furnace71

Page 72: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Chromatography Service Center

(Use Rate)

•Dionex Ion Chromatographs

•Dionex HPLC

•GCD

•GPC (Waters)

•Wyatt Light Scattering

•GCs

•GC-MS

72

Page 73: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Thermal Analysis Center

(Use Rate)

•TGA

•DSC

•DMA

•Rheometer

•FTIR

73

Page 74: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

NMR Service Center

(Use Rate)

NMR

74

Page 75: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Orders for Chemicals and

Supplies

•Rojas and Hubbe students: Carlos Salas

•Argyropoulos, Kelley, and Lucia students:

Lily Xiao

•Park students: Jun Park

•Heitmann, Jameel, Byrd, Kocurek, Venditti,

Pawlak: Linda McMurray

•Wood Products: Melissa Rabil

75

Page 76: LAB SAFETY - Site · Lab Rules: • Know the safety rules of the lab. • Do not use equipment without training. • Clean up! • Ask permission to borrow anything. • If you break

Good luck with your research!

76