keragaman dan karakterisasi mikroalga dari sumber air ... · identifikasi jenis-jenis mikroalga di...

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Lampiran 1 Lokasi pengambilan sample mikroalga

A. Ciater (pH = 2, Suhu = 45o C) B. Cipanas (pH = 7,6 , Suhu = 53o C)

C. Ciwalini (pH = 6,9 , suhu = 49o C) D. Gunung Pancar (pH = 7,5 , suhu=48)

Lampiran 2 Kandungan unsur hara empat lokasi penelitian.

No Lokasi C N P K Ca Mg Fe Cu Zn Mn

1 Ciater 1,536 55,72 2,98 29,00 0,96 2,85 0,49 0,03 0,11 1,88

2 Ciawalini 1,024 27,86 1,28 26,42 20,48 8,69 0,18 tr tr 0,22

3 Cipanas 942 69,38 0,93 5,06 0,09 tr 0,22 tr tr tr

4 Gunung Pancar 1,536 27,86 0,74 17,75 25,51 8,89 0,28 0,05 0,08 0,15

(ppm)

Keterangan : tr = tidak terukur

Bahan KimiaIMK BG 11 MBM Zarrouk

NaNO3 0,2 1,5 2,5 2,5CaCl2 _ _ _ 0,04NaCl _ _ 0,1 _Na2HPO4 1.4 x10-3 _ _ _KH2PO4 _ 0,175 _

K2HPO4 5 x 10-3 0,04 0,075 0,5Na2SO4 _ _ _ 1NaHCO3 _ _ _ 16,8Na2CO3 _ 0,02 _EDTA _ _ _ 0,08NH4Cl 2.68 x 10-3

_ _ _

Fe-EDTA 5.2 x 10-3 _ _ _Mn-EDTA 3.32 x 10-4 _ _ _Na2-EDTA 3.72 x 10-2 0,004 _ _

ZnSO4.7H2O 2.3 x 10-6 0,222 0,222 0,222CaCl2.2H2O _ 0,036 1 _

CoSO4.7H2O 1.4 x 10-5_ _ _

Na2MoO4.H2O 7.3 x 10-8 0,39 _ _

CuSO4.5H2O 2.5 x 10-6 0,079 0,075 0,075MgSO4.7H2O _ 0,075 0,075 0,2MnCl2.4H2O _ 1,81 1,81 1,81Co (NO3)2.6H2O _ 0,0494 _ _(NH4)6.Mo7O24.4H2O _ 0,018 0,018H3BO3 _ 2,86 2,86 2,86H2SeO3 1.7 x 10 -6

_ _ _Fe- Amonium Citrate _ 0,006 _ _FeSO4 _ _ _ 0,01Fe-Citrate _ _ 1 _Citric Acid _ 0,006 _ _Thiamin HCl 2 x 10 -4 _ _ _Biotin 1.5 x 10-6 _ _ _Vitamin B12 1.5 x 10-6 _ _ _MnCl2.4H2O 1.8 x 10-5

_ _ _

Media (g/l)

Lampiran 3 Nama dan komposisi bahan kimia media tumbuh mikroalga

Lokasi Nama Spesies Divisi Jumlah Spesies KelimpahanCiater Chroococcus Cyanophyta 6 30

Galdiera sp Chlorophyta 53Staurastrum Chlorophyta 12Tribonema Crysophyta 33Tetraselmys sp Chlorophyta 17Melosira Bacillariophyta 9

Cipanas Tetraselmys sp Chlorophyta 9 200Anacystis Cyanophyta 30Navicula Bacillariophyta 76Chroococcus Cyanophyta 20Microcystis Cyanophyta 66Nannochloris Chlorophyta 60Synedra Bacillariophyta 12Scenedesmus Chlorophyta 58Chlorococcum sp Chlorophyta 50

Ciwalini Nannochloris sp Chlorophyta 12 80Scenedesmus sp Chlorophyta 56Synechococcus Cyanophyta 23Staurastrum Chlorophyta 30Synedra Bacillariophyta 9Tetraselmys Chlorophyta 78Merismopedia Cyanophyta 36Oscillatoria Cyanophyta 25Chroococcus Cyanophyta 87Goniochloris Cyanophyta 8Rhizosolenia Bacillariophyta 35Navicula Bacillariophyta 21

Gunung Nannochloris sp Chlorophyta 8 32Pancar Navicula Bacillariophyta 78

Chlorosarcinopsis sp Chlorophyta 80Tetraselmys sp Chlorophyta 26Diatom vulgare Bacillariophyta 38Chroococcus sp Cyanophyta 76Anacystis nidulans Cyanophyta 12Microsystis Cyanophyta 7

Lampiran 4 Nama spesies dan kelimpahan mikroalga yang teridentifikasi

Lampiran 5 Gambar mikroalga dari sumber air panas Ciater dan Cipanas, denganperbesaran 400x.

Ciater

Chroococcus sp. Galdiera sp. Staurastrum Tribonema

Melosira Tetraselmis

Cipanas

Tetraselmis sp. Anacystis sp. Navicula sp. Chroococcussp.

Mycrocistis sp. Nannochloris sp Synedra Scenedesmus

Chlorococcum sp

Lampiran 6 Gambar mikroalga dari sumber air panas Ciwalini dan GunungPancar, dengan perbesaran 400x

Ciwalini

Nannochloris sp. Scenedesmus sp. Synechococcus sp. Tetraselmissp.

Oscilatoria sp. Chroococcus sp. Rhizosolenia sp. Navicula sp.

Synedra Merismopodia Goniochloris Tetraselmis

Gunung Pancar

Nannochloris sp. Tetraselmis sp. Diatom vulgare Chroococcus sp.

Anacystis sp. Microcystis sp. Chlorosarcinopsis sp. Tetraselmys sp.

PerlakuanNitrogen (M) F35 F70 F140N0.5 1.017 d 1.204 dc 1.455 bc

N1.0 1.066 d 1.119 d 1.526 b

N2.0 0.811 d 0.893 d 2.480 a

Cahaya (µmol foton/m2 /detik)

Ulangan 2 0.38352363 0.19176181Cahaya (F) 2 3.9726903 1.98634515 67.96 0.0001 **

Galat (F) 4 0.29144793 0.07286198Nitrogen (N) 2 0.17445452 0.08722726 2.98 0.0887 tnF x N 4 2.13362104 0.53340526 18.25 0.0001 **

Galat (N) 12 0.35071844 0.02922654Total 26 7.30645585

SumberKeragaman

DB JK KT F hitung Pr > F

Lampiran 7 Analisis sidik ragam pertumbuhan mikroalga Galdiera sp. pada umurPada umur 14 hari

Keterangan : tn = tidak berbeda nyata

** = berbeda sangat nyata

Uji lanjut dengan LSD pada α = 0,05

Keterangan : Angka yang diikuti huruf yang sama menunjukkan tidak berbedanyata.

Ulangan 2 0.25883267 0.12941633 0.0138Cahaya (F) 2 9.52704422 4.76352211 230.28 0.0001 **

Galat (F) 4 0.13336178 0.03334044Nitrogen (N) 2 1.349864 0.674932 32.63 0.0001 **

F x N 4 0.36535178 0.09133794 4.42 0.02 **

Galat (N) 12 0.24823222 0.02068602Total 26 7.30645585

KT F hitung P > FSumberKeragaman

DB JK

PerlakuanNitrogen (M) 35 70 140

N0.5 1.359 g 2.687 c 2.661 cd

N1.0 1.192 h 2.495 ed 2.826 bc

N2.0 2.029 f 2.881 b 3.113 a


Lampiran 8 Analisis sidik ragam pertumbuhan mikroalga Chlorococcum sp. padaumur 14 hari.

Keterangan : ** = berbeda sangat nyata



Ulangan 2 0.03660452 0.01830226Cahaya (F) 2 2.99521563 1.49760781 90.23 0.0001 **


F x N 4 0.6493137 0.16232843 9.78 0.0009 **

Galat (N) 12 0.19916378 0.01659698Total 26 5.53673519

SumberKeragaman

DB JK KT F hitung P > F


N0.5 0.64 e 1.33 c 1.31 c

N1.0 0.91 d 1.26 c 1.88 b

N2.0 1.39 c 1.39 c 2.18 a


Lampiran 9 Analisis sidik ragam pertumbuhan mikroalga Synechococcus sp. padaPada umur 14 hari.





N0.5 0.69 g 0.62 g 1.25 e

N1.0 1.05 f 1.79 d 2.39 b

N2.0 1.12 e 2.23 c 2.75 a


Lampiran 10 Analisis sidik ragam pertumbuhan mikroalga Chlorosarcinopsis sp.pada umur 14 hari.

Ulangan 2 0.00386052 0.00193026Cahaya (F) 2 6.19085007 3.09542504 499.89 0.0001 **


F x N 4 1.35253437 0.33813359 54.61 0.0001 **

Galat (N) 12 0.07430644 0.0061922Total 26 14.41270296

KT F hitung P > FSumberKeragaman

DB JK


Uji lanjut dengan LSD pada pada α = 0,05


Ulangan 2 1.68518519 0.84259259Cahaya (F) 2 6.74074074 3.37037037 14.86 0.0006 **Galat (F) 4 0.42592593 0.10648148Nitrogen (N) 2 0.7962963 0.39814815 1.76 0.2145 tnF x N 4 2.48148148 0.62037037 2.73 0.0791 tnGalat (N) 12 2.72222222 0.22685185Total 26 14.85185185

Pr > FSumberKeragaman

DB JK KT F value


N0.5 8.33 9.17 10.00

N1.0 8.50 9.17 8.83

N2.0 8.50 9.00 10.17

Rata-rata 8.44 c 9.11 b 9.67 a

Cahaya (µmol fhoton/m2 /detik)

Lampiran 11 Analisis sidik ragam produksi biomasa mikroalga Galdiera sp.

Keterangan : tn = tidak berbeda nyata** = berbeda sangat nyata


Ulangan 2 1.16666667 0.58333333 0.68 0.5229Cahaya (F) 2 15.05555556 7.52777778 8.84 0.0044 **

Galat (F) 4 9.61111111 2.40277778 2.82 0.0732Nitrogen (N) 2 63.16666667 31.58333333 37.08 0.0001 **

F x N 4 24.94444444 6.23611111 7.32 0.0032 **

Galat (N) 12 10.22222222 0.85185185Total 26 124.1666667

SumberKeragaman

KT F valueDB JK Pr > F


N0.5 8.000 bc 10.333 ab 7.333 dc

N1.0 11.500 a 12.333 a 9.333 b

N2.0 10.833 a 12.333 a 13.500 a


Lampiran 12 Analisis sidik ragam produksi biomassa mikroalga Chlorococcumsp.




Ulangan 2 0.72222222 0.36111111Cahaya (F) 2 2.05555556 1.02777778 3.83 0.0518 tnGalat (F) 4 2.88888889 0.72222222Nitrogen (N) 2 2.05555556 1.02777778 3.83 0.0518 tnF x N 4 4.55555556 1.13888889 4.24 0.0228 *Galat (N) 12 3.22222222 0.26851852Total 26 15.5

SumberKeragaman

DB JK KT F value Pr > F


N0.5 5.500 b 6.000 b 6.000 b

N1.0 5.833 b 6.167 b 5.333 b

N2.0 5.500 b 6.333 b 7.333 a


Lampiran 13 Analisis sidik ragam produksi biomassa mikroalga Synechococcussp.

Keterangan : tn = tidak berbeda nyata** = berbeda sangat nyata



Ulangan 2 1.46296296 0.73148148Cahaya (F) 2 8.90740741 4.4537037 16.31 0.0004 **Galat (F) 4 0.59259259 0.14814815Nitrogen (N) 2 19.12962963 9.56481481 35.02 0.0001 **F x N 4 4.25925926 1.06481481 3.9 0.0297 *Galat (N) 12 3.27777778 0.27314815Total 26 37.62962963

SumberKeragaman


PerlakuanNitrogen (M) 35 70 140N0.5 6.33 c 7.33 bc 6.50 c

N1.0 6.83 c 8.17 b 8.67 b

N2.0 7.83 b 8.67 b 9.83 a


Lampiran 14 Analisis sidik ragam produksi biomassa mikroalgaChlorosarcinopsis sp.

Keterangan : * = berbeda nyata** = berbeda sangat nyata


Keterangan : Angka yang diikuti huruf yang sama menunjukkan tidak berbeda

nyata.

Ulangan 2 0.00162222 0.00081111Cahaya (F) 2 0.02195556 0.01097778 12.64 0.0011 **


F x N 4 0.02088889 0.00522222 6.01 0.0068 *

Galat (N) 12 0.01042222 0.00086852Total 26 0.0846

KT F value Pr > FSumberKeragaman

DB JK


N0.5 0.127 d 0.230 b 0.280 a

N1.0 0.117 d 0.163 cd 0.143 d

N2.0 0.163 cd 0.147 d 0.190 cb


Lampiran 15 Analisis sidik ragam kandungan lipid mikroalga Galdiera sp.

Keterangan : * = berbeda nyata**= berbeda sangat nyata



nyata.

Ulangan 2 0.00074074 0.00037037Cahaya (F) 2 0.03851852 0.01925926 26 0.0001 **


F x N 4 0.0437037 0.01092593 14.75 0.0001 **

Galat (N) 12 0.00888889 0.00074074Total 26 0.12296296

SumberKeragaman



N0.5 0.100 c 0.300 a 0.200 b

N1.0 0.100 c 0.200 b 0.200 b

N2.0 0.133 c 0.100 c 0.133 c


Lampiran 16 Analisis sidik ragam kandungan lipid mikroalga Chlorococum sp.




nyata.

Ulangan 2 0.00006667 0.00003333Cahaya (F) 2 0.00275556 0.00137778 18.6 0.0002 **


F x N 4 0.00242222 0.00060556 8.18 0.002 **

Galat (N) 12 0.00088889 0.00007407Total 26 0.0204


DB JK


N0.5 0.090 c 0.120 b 0.147 a

N1.0 0.067 c 0.073 c 0.070 c

N2.0 0.063 c 0.073 c 0.077 c


Lampiran 17 Analisis sidik ragam kandungan lipid mikroalga Synechococcus sp.


uji lanjut dengan LSD pada α = 0,05


nyata.

Ulangan 2 0.00031852 0.00015926Cahaya (F) 2 0.00796296 0.00398148 59.72 0.0001 **Galat (F) 4 0.00048148 0.00012037Nitrogen (N) 2 0.00794074 0.00397037 59.56 0.0001 **F x N 4 0.00659259 0.00164815 24.72 0.0001 **Galat (N) 12 0.0008 0.00006667Total 26 0.0240963


DB JK

PerlakuanNitrogen (M) 35 70 140N0.5 0.117 fg 0.203 a 0.170 b

N1.0 0.120 f 0.123 ef 0.153 c

N2.0 0.110 g 0.137 d 0.123 ef


Lampiran 18 Analisis sidik ragam kandungan lipid mikroalga Chlorosarcinopsissp.


uji lanjut dengan LSD pada α = 0,05


Ulangan 2 0.00074074 0.00037037Cahaya (F) 2 0.01291852 0.00645926 25.65 0.0001 **


F x N 4 0.00992593 0.00248148 9.85 0.0009 **

Galat (N) 12 0.00302222 0.00025185Total 26 0.0388963

Pr > FSumberKeragaman

DB JK KT F value


N0.5 0.067 c 0.133 b 0.177 a

N1.0 0.063 c 0.097 c 0.080 c

N2.0 0.087 c 0.080 c 0.120 bc


Lampiran 19 Analisis sidik ragam produktivitas lipid mikroalga Galdiera sp.




nyata.

Ulangan 2 0.00074074 0.00037037Cahaya (F) 2 0.01851852 0.00925926 25 0.0001 **

Galat (F) 4 0.00148148 0.00037037Nitrogen (N) 2 0.00518519 0.00259259 7 0.0097 **

F x N 4 0.01037037 0.00259259 7 0.0038 **

Galat (N) 12 0.00444444 0.00037037Total 26 0.04074074

DB JK Pr > FSumberKeragaman

KT F value


N0.5 0.100 c 0.200 a 0.100 c

N1.0 0.100 c 0.167 b 0.100 c

N2.0 0.100 c 0.100 c 0.100 c


Lampiran 20 Analisis sidik ragam produktivitas lipid mikroalga Chlorococcum sp.


Uji lanjut denan LSD pada α = 0,05


Ulangan 2 0.0000963 0.00004815Cahaya (F) 2 0.00111852 0.00055926 23.23 0.0001 **


F x N 4 0.00045926 0.00011481 4.77 0.0155 *

Galat (N) 12 0.00028889 0.00002407Total 26 0.00396296

DB JK KT F value Pr > FSumberKeragaman


N0.5 0.030 cd 0.047 b 0.057 a

N1.0 0.023 e 0.030 cd 0.027 de

N2.0 0.020 e 0.027 de 0.037 c


Lampiran 21 Analisis sidik ragam produktivitas lipid mikroalga Synechococcussp.

Keterangan : * = berbeda nyata** = berbeda sangat nyata

Uji lanjut dengan LSD pada pada α = 0,05


Ulangan 2 0.00034897 0.00017448Cahaya (F) 2 0.00403234 0.00201617 49.54 0.0001 **Galat (F) 4 0.00019416 0.00004854Nitrogen (N) 2 0.00006824 0.00003412 0.84 0.4563 tnF x N 4 0.00171824 0.00042956 10.55 0.0007 **Galat (N) 12 0.00048839 0.0000407Total 26 0.00685033

DB JK KT F value Pr > FSumberKeragaman


N0.5 0.046 e 0.093 a 0.070 b

N1.0 0.051 cd 0.063 b 0.083 b

N2.0 0.054 cb 0.074 b 0.076 b


Lampiran 22 Analisis sidik ragam produktivitas lipid mikroalgaChlorosarcinopsis sp.

Keterangan : tn = tidak nyata**= berbeda sangat nyata



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