Isotopic Analysis of Bothrops atrox in Amazonian ForestMELISSA GASTE MARTINEZ1 ANA MARIA MOURA DA SILVA2 HIPÓCRATES CHALKIDIS3 RAIMUNDO COSME DE OLIVEIRA4 PLÍNIO BARBOSA DE

CAMARGO5

1. Isotopic Ecology Lab, Center of Nuclear of Energy in Agriculture CENA/USP, Piracicaba, Sao Paulo, Brazil.2. Butantan Institute, Sao Paulo, Sao Paulo, Brazil.3. Zoology, Integrated Colleges Tapajos, Santarem, Para, Brazil.4. CPATU, Embrapa, Santarem, Para, Brasil5. Isotopic Ecology Lab, Center of Nuclear of Energy in Agriculture CENA/USP, Piracicaba, Sao Paulo, Brazil.

Introduction

Methods

Bothrops atrox

In Brazil, the 26,000 snakebites, 90% are of the genus Bothrops, and Bothrops atrox species predominant in the Amazon region including all the Brazilian Amazon. Research shows that using stable isotopes, we can verify the isotopic composition of tissues of animals that depend mainly on food, water ingested and inhaled gases.

ObjectivesFor this study, samples taken from Bothrops atrox (B. atrox) in forest using pitfall traps and fall ("fall-Pitt traps with drift fence"), were to analyze the feeding habits of Bothrops atrox in different locations, testing a correlation between diet and the composition of the venom of this species and also work with animals collected in areas with different land uses such as native forest, savannah and agricultural areas (pasture) in the region of Santarém, PA, Amazonia. with the use of traps and trapping fall (pit fall traps).

Selection of the Sampled areas

For this study, samples taken from Bothrops atrox (B. atrox) in three areas with different vegetation cover:1. Forest.

2. Savanna. 3. Agricultural land (pasture)

Plateau Shoal

Photo 1: Preserved area Photo 2: Area conserved

Photo 3: Savanna, Tapari Photo 4: agricultural land (pasture)

Methods of collection:1. Interception traps and fall ("pit-fall traps with drift fence"). 2. Active visual search time limited. 3. Casual Encounter.Isotopic analysis