Intra-Cytoplasmic Sperm Injection – ICSI

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Injecting A Single Sperm Into An Egg

Possible Even With A Small Number Of Sperm

To Fertilize Egg In IVF Lab

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Specialized tools and inverted microscopes

Pick up a sperm using a specialized ICSI needle Advanced through the egg membrane

The sperm is injected into the inner part of the egg (the cytoplasm).

Then fertilization occurs in 75 - 85% of eggs injected with sperm.

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Indications Of I C S I

Male infertility factors that includes low sperm counts, poor motility or movement of the sperm, poor sperm quality, sperm that lack the ability to penetrate an egg or azoospermia.

Failure of fertilization in previous IVF attempts.If the male partner underwent a vasectomy and sperm have

been collected from the testicles or epididymis.If male partner has problems in erection or ejaculation.It is used for couples with a small number of eggs at egg

retrieval, to get a higher percentage of eggs fertilized than when mixing eggs and sperms together in the IVF procedure.

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The Procedure Of I C S I

Stimulation Of Ovaries Collection Of Eggs by hollow needle &

ultrasound guidance. Sperm Picking by delicate needle. Transferred to the uterus 2 to 3 days

after fertilization. Pregnancy test should be done 2 weeks

after the embryo transfer.

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Methods of sperm retrieval for I.C.S.I If there were sperm in the semen, a sperm

sample can be produced by normal ejaculation into a cup on the same day of egg collection.

If there is no sperm in the semen, the doctor can extract sperm from the male partner under local or general anesthesia.

Percutaneous Epididymal Sperm Aspiration & I C S I (P.E.S.A , M.E.S.A)It is only required if the husband has one of the following problems: Absence of the vas deferens (through which the

sperm passes from the epididymis to the seminal fluid ).

Past Infections that result in obstruction. Cases who have had a vasectomy and surgical

reversal failed.

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Testicular Sperm Aspiration & I.C.S.I (T.E.S.A, T.E.S.E) Obtaining sperm directly from the

testes, either by aspiration, using a needle or surgical extraction

The sperm are then used to do the ICSI procedure as described above.

This method is used in the following cases: Absence of sperms in the epididymis. Absent or obstructed epididymis. TESE might be done before the

beginning of the treatment cycle and the collected sperm are then frozen.

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Disadvantages Of I C S I The risk of multiple births and ectopic

pregnancy. The risk of congenital abnormalities. There is an increased risk of genetic

problem carried by the sperm. ICSI is more expensive than IVF.

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