industrial biotechnology, past, present, and future swedish-african partnerships
TRANSCRIPT
Industrial biotechnology, past, present and future Swedish-
African Partnerships.
Bo Mattiasson
Lund University and Indienz AB
Sida-supported activities
• BioEARN
• BioInnovate
• Bilateral programs
BioEARN and to some extent also in BioInnovate
• Enzyme and microbial technology
• Environmental biotechnology
Projects
• Upgrading fish waste – production of PUFA
• Biogas from industrial waste
• Enzymes for the leather industry
• Wastewater treatment e.g. from leather industry
Biogas from surplus biomass
Anaerobic degradation of organic material
ORGANIC MATERIAL
SOLUBLE MONOMERS
EXTRACELLULAR
HYDROLYSIS
VOLATILE FATTY
ACIDS
ACETATE HYDROGEN
CARBON DIOXIDE
ACIDOGENESIS
ACETOGENESIS
METHANOGENESIS
METHANE
CARBON DIOXIDE
Enzymatic degradation
Microbial degradation
BIOGAS
METHANOGENESIS
How far can one drive on the
bioenergy produced from 1 hectar
(10.000 m2)?
Project with Tanzania
• Bioenergy carriers from biomass
• Ethanol from wild cassava
• Biogas from residues from starch production
Biomass can be used for many purposes
Platform chemcials
• For industry to build on.
• Relatively simple molecules that are converted afterwards
Sources of biomass
• Waste from traditional farming
• Waste from industrial processing
• Municipal solid waste
• GMO crops specially designed for industrial biotech.
• Algae
• etc
12
3
Mono-, di-, or tri- glycerate
Glycerol carbonate
Glyceric acid
3-Hydroxypropionic acid
1,3-Propanediol
Propionic acid
3-Hydroxypropionaldehyde
Propanol
Glycerol
Glycidol
DihydroxyacetoneDiglyceraldehyde
Branched polyesters and nylons
1,2-Propanediol
Malonic acidAcrylic acid
Ethyl-3HP
Methylacrylate
Acrylamide
2
Acrylonitrile
3-Hydroxypropionic acid
Propiolactone
Poly-3HP
1,3-Propanediol
Enzymes and micororganisms as industrialcatalysts
• Organisms isolated from extreme environments are a good source of enzymes adapted to harsh conditions
• Enzyme properties can be improved using gene technology
A novel alkaline protease from a novel alkaliphilic microorganism isolated from an alkaline soda lake
Nesterenkonia aethiopica Protease crystals
New biology to obtain suitable Biocatalysts
RESEARCH APPROACH
Extremophilicorganisms,
Genesisolation
cultivation
Extreme habitats
Cloning, expression & Production
Rational design,Directed evolution
genes
GREENCHEM
Industrial enzymes
• For food industry
• For leather industry
• For chemical industry
• For cosmetics
• For conversion of biomass
• etc.
Environmental biotechnology
• Remediation
• Degradation of toxic chemicals
• Bioenergy (mainly biogas)
The largest biotech process
The largest biotech process
• Treatment of water
– Wastewater
– Water for consumption
Heavy metals constitute a serious environmental problem today!
• Polluting water and soil
• HM are taken up by the crops
• Influences ecosystems negatively
Bioreactors for removal of heavy metal ions
• Sulfate reducing bacteria grown in bioreactor
• Porous glass used as carrier material
worldwide public
health problem
Microcystins
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Collaboration with the private sector?!
STRATEGY FOR BUILDINGSTRONG BIOTECHNOLOGY
RESEARCH.
Microbialfermentation
Enzymetechnology
Enzyme µbial
technology
Cloning & molecularbiology
Downstreamprocessing ofbiomolecules
Anaerobic fermentation
Processtechnology
The seven pillars for building competence inIndustrial biotechnology.
Microbialfermentation
Enzymetechnology
Enzyme µbial
technology
Cloning & molecularbiology
Downstreamprocessing ofbiomolecules
Anaerobic fermentationProcess
technology
Need for adding application oriented work
Microbialfermentation
Enzymetechnology
Enzyme µbial
technology
Cloning & molecularbiology
Downstreamprocessing ofbiomolecules
Anaerobic fermentationProcess
technology
Need for adding APPLIED RESERCH
Enzymetechnology
Applied projects for e.g. Master students
Enzymetechnology
Applied projects for e.g. Master students
Industrial partner
Enzymetechnology
Applied projects for e.g. Master students
Industrial partner
Fermentationtechnology
Microbialfermentation
Enzymetechnology
Enzyme µbial
technology
Cloning & molecularbiology
Downstreamprocessing ofbiomolecules
Anaerobic fermentationProcess
technology
Add INDUSTRIALCOLLABORATION
Course in entrepreneurship in biotechnology
• Make a market survey
• Write a business plan
• To arrange financial support
• IPR
• To meet others who have made the journey and succeeded or failed.
Why this course?
• Important to discuss project areas that are familiar to the students.
• Important to have concrete objects to work with
• It gives the students the toolbox for starting
In Lund this has resulted in
• Bioprocess Control AB
• Capsenze HB
• Enviro AB
• Protista Int. AB
• Indienz AB
• Cyclicor AB
• Enza AB
• m.fl.
Indienz ABworks with a pilot scale biorefinery
• One of the founders now tries to start a daughter company in India
• One founder from Ethiopia is investigating the possibilities to establish activities in Ethiopia.
Bioprocess Control AB
monitoring and control of anaerobic digestion
• On founder from China is CEO and has started a daughter company there.
Points to focus on …
• Postdoctoral training
• Industrial contacts
• PhD-programme and postdoc programmes to be introduced gradually
When starting …
• Collaborate with established companies
• Start with less demanding projects
• Consulting, trouble shooting etc
Thank you for listening!
Bo Mattiasson
• www.biotek.lu.se
• +46 70-6059830