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Prepared by Lawrence Kok

Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC.

Click here to view TLC and Column ChromatographySeparation using TLC TLC techniques step by step

Column Chromatography Column chromatography separation

Click here to view HPLC and Gas Liquid Chromatography

HPLC works Gas Liquid Chromatography works

Chromatography

Partition Chromatography vs Adsorption Chromatography

Partition Chromatography Adsorption Chromatography

Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html

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Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html

Mobile phase - Liquid Mobile phase - Liquid

Stationary phase - Liquid Stationary phase - Solid

Partition Chromatography Adsorption Chromatography

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Distribution of X, Y and Zbetween two liquid phases

Adsorption of X, Y and Z onto SOLID phase

Type of chromatography

Type of chromatography

Type of chromatography

Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)

Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)

Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)

Type of chromatography

Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)

Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)

Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)

Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent

Type of chromatography

Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)

Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)

Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)

Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent

Solvent moves by capillary actionPartition/Distribution of solutebetween 2 liquid phase

Adsorption of solute onto Stationary solid phase

Adsorption of solute on Stationary phase- strongly adsorp – travel slowly (eluted last)- weakly adsorp – travel fast (eluted first)

Separated components measured using Rf values

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Click here to viewSeparation using TLC TLC techniques step by step

Column Chromatography Column chromatography separation

Type of chromatography

Type of chromatography

We also have HPLC and GLC

Pic from:http://www.comsol.com/stories/waters_corp_hplc_systems/full/ Pic from:http://www.sfu.ca/bisc/bisc-429/GLC.html

High Performance Liquid Chromatography Gas Liquid Chromatography

High Performance Liquid Chromatography Gas Liquid Chromatography

Click here to view

HPLC works Gas Liquid Chromatography works

Summary

Paper Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid)

Thin Layer Chromatography - Adsorption Chromatography- Mobile phase (Liquid)- Stationary phase (Solid)

Column Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid)

High Performance Liquid Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid)

Gas Liquid Chromatography – Partition Chromatography - Mobile phase (Gas) - Stationary phase (Liquid)

Acknowledgements

Thanks to source of pictures and video used in this presentation

Thanks to Creative Commons for excellent contribution on licenseshttp://creativecommons.org/licenses/

Prepared by Lawrence Kok

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