Genetic Resources Core Facility (GRCF) Mission

To provide high quality, cost effective research services and products to investigators throughout the

Johns Hopkins Scientific Community.

Contents

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GRCF Services

Each of the GRCF’s Services will have an exhibit table with representatives to answer questions.

Scientific Exhibitors

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Fifteen of the GRCF’s Corporate Partners will have exhibit tables. Please visit their exhibits to learn about their products and services.

Plenary Session

Kevin  V.  Shianna,  Ph.D.,  Sequencing  Specialist,    Illumina,  Inc.  

GRCF Services

Core Store

The Core Store provides one-stop shopping for more than 250,000 products from 16 of the leading life science companies. In addition to its product offering, the store charges no shipping and handling fees and has free delivery to three campuses East Baltimore, Bayview and Homewood. There is also convenient 24/7 access to several hundred products via the Core Store 24/7 at these locations Blalock 1026, CRB I B02A and the Asthma and Allergy Building 1A.C4. Please visit us at http://grcf.jhmi.edu/core-store/.

grcf2

CORE SYMPOSIUM – March 3rd, 2015, 10 a.m. – 2:15 p.m.

Turner Concourse

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Keynote Address

Loyal Goff, Ph.D., Institute of Genetic Medicine, Depart- ment of Neuroscience, Johns Hopkins University

Attend a Seminar

grcf.jhmi.edu

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

The Biorepository & Cell Center facilitates basic scientific research by providing expertise and service in all mammalian cell culture and long-term cryogenic storage of biospecimens. In 2013 the GRCF Biorepository & Cell Center proudly received the international recognition of CAP (the College of American Pathologist) Accreditation. This voluntary program demands that a laboratory go well beyond acceptable quality and regulatory compliance measures to help achieve the highest standards of excellence in our services. Please visit us at http://grcf.jhmi.edu/biorepository-cell-center/

The DNA Services group works together to provide solutions for all of your DNA and RNA needs. We handle basic needs like DNA isolation, plating and storage, “traditional” core services like Sanger sequencing, PCR support and genotyping, as well as more complex needs presented by the constantly changing field of next generation sequencing. For more information on these services please visit us at http://grcf.jhmi.edu

Scientific Exhibitors

Affymetrix

Agilent

Bio-Rad Laboratories

Cell Signaling Technology

Corning Cellgro

Fluidigm Corporation

GE Healthcare Life Sciences

Integrated DNA Technologies (IDT)

Illumina, Inc.

New England Biolabs, Inc.

QIAGEN

Quality Biological, Inc.

Roche Applied Science

Sigma-Aldrich

Thermo Fisher Scientific

GRCF Services

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

Keynote Address – 11:30 a.m. – 12:30 p.m.

Title: “Deep transcriptional profiling of neurons during development and disease: Revealing hidden layers of information”

Room: Tilghman Auditorium Time: 11:30 a.m. – 12:30 p.m. Sponsor: GRCF

Presented by: Loyal Goff., Ph.D., McKusick-Nathans Institute of Genetic Medicine, Department of Neuroscience, Johns Hopkins University School of Medicine

Abstract: The inherent complexity of the mammalian brain has confounded many attempts to establish a comprehensive ‘parts list’ of key cell types, the mechanisms through which they are specified, and their distinct responses to various conditions. Several recent technologies and approaches have been developed that permit the specific isolation of distinct neuronal subtypes from this heterogeneous tissue. We have developed one such approach to purify three developmentally related populations of mouse cortical projection neurons at select time points during cortical development. By combining this enrichment with deep RNA-Sequencing we are able to explore the gene expression changes underlying the specification and development of these closely related cell types, discover thousands of previously uncharacterized non-coding RNAs, and characterize the various transcriptional regulatory events that contribute to specific cellular identities. In a related focus, we are beginning to assess the variability of individual cellular responses during neuronal differentiation through single-cell sequencing of human iPSC. By leveraging the power of single cell RNA-Seq we can identify key cellular decisions that serve to direct lineage commitment and cell fate choices, and ascertain specific genes and pathways that are disrupted in disease.

Plenary Address – 12:30 p.m. – 1:15 p.m.

Title: “Advances in RNA-seq: Complete workflow solutions”

Room: Tilghman Auditorium Time: 12:30 p.m. – 1:15 p.m. Sponsor: Illumina

Presented by: Kevin  V.  Shianna,  Ph.D.,  Sequencing  Specialist,  Illumina

Abstract: In the past, there were limitations to running RNA Seq expression studies. Today, these barriers have been lowered so that the typical bench scientist with no next generation sequencing experience or bioinformatics support/expertise can successfully run a well powered RNA seq experiment. These advances will be discussed with a focus on appropriate project design, sequencing recommendations and a demonstration of easy to use RNA seq analysis apps on Illumina's cloud computing resource (BaseSpace).  

 

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

Seminar Directory – 10:15 a.m. Title: “Translating Genomic Discoveries to the Clinic: The Right Tool for the Job”

Room: West Room Time: 10:15 a.m. Sponsor: Affymetrix

Presented by: Meg Mintz Kelly, Field Application Scientist, Affymetrix

Abstract: During the workshop, we will discuss strategies to determine bio-markers for disease based on a whole genome approach. We will demonstrate how to obtain biological results at the gene or exon level using next generation Gene Chip Human & Mouse Transcriptome Arrays in a simple, 4-step workflow using the free Transcriptome Analysis Console (TAC) software. Discussions will also include: 1. Data analysis of alternative splicing events predicted by HTA and the downstream validation with our leading VeriQuest qPCR reagents and mid-throughput Quantigene Plex platform, integrated with Affymetrix microarray workflows. 2. Move into single-cell analysis with eBioscience’s highly specific RNA in situ hybridization methods: QuantiGene ViewRNA, and Quantigene FlowRNA. These targeted approaches will allow researchers, doctors and clinicians to design diagnostic methods and systems leading to personalized therapeutic treatment plans, optimizing results.

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Title: “CRISPR CRAZE: Be a part of the CRISPR Revolution!”

Room: MRB-G03 Time: 10:15 a.m. Sponsor: Sigma-Aldrich

Presented by: Joe Frangipane, Ph.D.., Regional Manager, Sigma-Aldrich

Abstract: This will be a technical seminar discussing CRISPRs, the cutting edge technology for modulating gene expression through making knockouts and knock-ins. We will summarize the history of genome editing starting with conventional methods to Zinc Finger Nucleases to CRISPRs. We will discuss new advancements and experimental data surrounding CRISPRs. Lastly, we will present the variety of CRISPR vectors and formats offered by Sigma-Aldrich including our new lenti-CRISPRs. Please come to learn about this exciting technology and how Sigma's expertise can be the solution for your next project.

Title: "Conquering Roadblocks Associated with Stem Cell Differentiation and Disease Modeling"

Room: Tilghman Auditorium Time: 10:15 a.m. Sponsor: Thermo Fisher Scientific

Presented by: Navjot Kaur, Ph.D., Staff Scientist at Cell Biology and Stem Cell Systems Group, Thermo Fisher Scientific

Abstract: Thermo Fisher Scientific, with its recently completed acquisition of Life Technologies, stands committed to providing tools and resources that help enable stem cell scientists to realize the vast potential of stem cells. In this symposium, we will discuss challenges associated with stem cell differentiation and present solutions to more effectively initiate and manipulate differentiation towards various lineages. Specifically, we will offer solutions and tools to more effectively initiate and manipulate differentiation towards neural, cardiac, and endoderm lineages and demonstrate how genomic editing technologies can be used to specifically modify disease-relevant genes in stem cells.  

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

Title: “Overview and Update on Agilent NGS Target Enrichment and Data Analysis”

Room: MRB-G01 Time: 1:15 p.m. Sponsor: Agilent

Presented by: Josh Wang, Ph.D. | Field Applications Scientist, Agilent

Abstract: NGS target enrichment applications are not only powerful genetic tools to enable rapid discovery of SNPs, InDels and fusion transcripts etc in a focused set of genes, exons and/or genomic regions, but also emerging molecular diagnostic tools to screen known mutations and identify novel mutations in genetic diseases such as cancer and inherited diseases. With over 600 publications and widest selection of workflows and DNA/RNA target panels, Agilent Technologies is the leading provider for target enrichment

applications in both basic and clinical fields.

Seminar Directory – 1:15 p.m.

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Title: “Advances in Western Blotting Technology: Bio-Rad’s Workflow, delivers more sensitive results, faster at a lower cost.”

Room: MRB-G03 Time: 1:15 p.m. Sponsor: Bio-Rad

Presented by: John Chuckalovcak, Ph.D, Field Specialist, Bio-Rad

Abstract: Although there have been significant advances in western blotting, imaging and reagent technologies to improve sensitivity, dynamic range of detection, and multiplexed target detection, the basic technique has remained essentially unchanged. In the past few years, journal editors and reviewers are requesting the quantitative interpretation of western blot data in terms of fold changes in protein expression between samples. Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the same linear dynamic range. Bio Rad has developed an elegant solution that addresses many of these hurdles in the western blotting process.

Title: “Roche-Nimblegen: A Complete Solution for Targeted Re-Sequencing”

Room: West Room Time: 1:15 p.m. Sponsor: Roche Diagnostics/Life Science

Presented by: James Furbee, Roche Diagnostics/Life Science

Abstract: Roche Nimblegen offers a comprehensive catalog of Sequence-Capture (targeted re-sequencing) products for all major next generation sequencing platforms. These include sequencing library prep for the Illumina platform (now available at the Core Store), DNA targeted capture for exome or gene panels, RNA targeted capture for gene expression quantification, and Methylated DNA capture (SeqCap Epi) for the study of methylated DNA. Come join us to see how Roche Nimblegen can improve your sequencing results and save you money in the process.

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

Symposium Promotions Keynote/Plenary  Address  Attendance  Promotion:  Enter  for  a  chance  to  win  an  iPad!    Here’s  how  you  can  participate.    

1.  Attend  from  start  to  end  the  Keynote  Address,  11:30  a.m.  -­‐  12:30  p.m.,  and/or  the  Plenary  Address,  12:30  p.m.  –  1:15  p.m.  Tilghman  Auditorium  

2.  Turn  in  a  completed  entry  card*  to  a  GRCF  staff  member  at  the  end  of  the  keynote  address.  

3.  One  entry  per  person  per  address.  

Seminar  Attendance  Promotion:    Enter  for  a  chance  to  win  a  GRCF  lunch  tote.    

1.  Attend  from  start  to  end  any  of  the  six  seminars  throughout  the  day.  

2.  Turn  in  a  completed  entry  card*  to  a  GRCF  staff  member  at  the  end  of  the  seminar.  

3.  One  entry  per  person,  per  seminar.  

*Entry  cards  may  be  obtained  on  March  3rd  prior  to  the  start  of  the  Keynote  Address/seminars  at  the  registration  table.    Additionally,  entry  cards  will  be  available  prior  to  the  Symposium  at  any  of  the  GRCF  Divisions  located  on  Blalock  10th  Floor.  

Core  Store:    There  will  be  multiple  Core  Store  promotions  running  concurrent  with  the  Symposium.    Check  out  http://grcf.jhmi.edu/promotions/  on  March  3rd  to  view  the  full  listing.  

Door  Prizes:    Be  sure  to  sign  in  at  the  registration  desk  to  be  eligible  for  one  of  our  door  prizes!  

 

Title: “Novel Reagent Solutions for Flow Cytometry”

Room: Tilghman Auditorium Time: 1:15 p.m. Sponsor: Thermo Fisher Scientific

Presented by: Jolene Bradford, MLS(ASCP)CM, CCy R&D Associate Director, Flow Cytometry Systems, Thermo Fisher Scientific/Life Technologies

Abstract: Life Technologies has developed a number of novel reagents and assays designed for the researcher using Molecular Probes® reagents to accelerate the ability to characterize cell function. This seminar will present recently developed assays and cutting edge reagents for flow cytometry applications including apoptosis, ROS, proliferation and site-specific antibody labeling. The Attune® NxT Cytometer will be introduced with novel applications of acoustic cytometry. This data-rich presentation will also provide tips and tricks on technique.

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Visit our website at http://grcf.jhmi.edu March 3, 2015, 10:00 a.m. – 2:15 p.m. p.m. p.m.A

Coming this Spring

Fluidigm’s C1 Technology

For more information call 410-614-5201 or e-mail Melissa Olson at molson14@jhmi.edu

CORE SYMPOSIUM SEMINAR SCHEDULE

Turner Concourse, March 3, 2015

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West Room

Affymetrix "Translating Genomic

Discoveries to the Clinic: The Right Tool

for the Job"

Roche Diagnostics/Life

Science "Roche-Nimblegen: A Complete Solution for

Targeted Re-Sequencing"

MRB-G01

Dark

Agilent "Overview and

Update on Agilent NGS Target

Enrichment and Data Analysis"

MRB-G03

Sigma-Aldrich "CRISPR CRAZE: Be a

part of the CRISPR Revolution""

Bio-Rad "Advancements in Western Blotting

Technology: Bio-Rad's Western Workflow

delivers more sensitive results"

Tilghman Auditorium

Thermo Fisher Scientific

"Conquering Roadblocks

Associated with Stem Cell

Differentiation and Disease Modeling"

Loyal Goff, Ph.D. "Deep transcriptional profiling of neurons during development

and disease: Revealing hidden layers of

information"

Illumina "Advances in RNA-seq:

Complete workflow solutions"

Thermo Fisher Scientific

"Novel Reagent Solutions for Flow

Cytometry"

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10:15 a.m.– 11:15 am

11:30 a.m. -12:30 p.m. KEYNOTE ADDRESS

1:15 p.m. – 2:15 p.m.

12:30 p.m. – 1:15 p.m., PLENARY ADDRESS

For more information visit us at grcf.jhmi.edu