gilson product guide

Gilson, Inc.

Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for today’s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendary Gilson Pipetman® products to a full range of system solutions, Gilson is the element of purification for your laboratory. Gilson’s innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson.

Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications.

Gilson offers complete solutions including the revolutionary control software, TRILUTION®. TRILUTION® LC Software is a complete HPLC software package designed specifically to meet purification needs. TRILUTION® LH Software is designed to make automation of liquid handling and SPE applications simple and dependable.

Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide.

Quality Policy

It is Gilson, Inc.’s policy to promote a total commitment to quality by each individual throughout the

Gilson organization worldwide. It is Gilson’s goal that this commitment to quality will result in

complete customer satisfaction with both Gilson’s products and services.

ISO 9001 Certification

Gilson is committed to quality, and it shows in everything Gilson does – from design to manufacturing,

technical support and training. Due to this commitment to quality and customer satisfaction, Gilson

has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Gilson S.A.S. are both

ISO 9001 certified.

Training

Gilson provides an extensive program of instrument and software training for users. Standard courses as

well as sessions customized to the user’s specific needs are offered at Gilson’s innovative training center or

in the user’s own laboratory. Computer assisted training courses are also available on CD-ROM or the Gilson

on-line education center, www.training.gilson.com.

Gilson, Inc. World Headquarters

3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA

Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451

[email protected] | [email protected] | [email protected] | [email protected] | [email protected]

Gilson S.A.S.

19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE

Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020

ANCE

HPLC: Systems 2 – 10GX-281 Preparative HPLC System ......................................... 5GX-281 Analytical to Semi-preparative

HPLC System ........................................................................... 6GX-271 Preparative HPLC System ......................................... 7GX-271 Analytical to Semi-preparative HPLC System ... 8845ZPREP™ Semi-preparative to Preparative HPLC System .................................................... 9cLC™ Analytical to Preparative HPLC System ................... 9GX-271 Fraction Trapping System ......................................10

HPLC: Liquid Handlers/Injectors 12 –16GX-281 Liquid Handler/Injector ..........................................13GX-271 Preparative Liquid Handler/Injector ..................14GX-271 Analytical Liquid Handler/Injector .....................15GX-271 Liquid Handler/Injector without pump ............15215 Liquid Handler/Injector .................................................16Multiple Probe 215 Liquid Handler/Injector ...................16

HPLC: Pumps 17 –21333/334 Prep-scale HPLC Pumps .......................................18331/332 Semi-preparative HPLC Pumps ..........................19321/322 Analytical to Semi-preparative

HPLC Pumps ..........................................................................1930X Series HPLC Pumps .........................................................20402 Syringe Pumps ..................................................................20Minipuls Evolution® Peristaltic Pump ...............................21Minipuls 3 Peristaltic Pump ..................................................21

HPLC: Detectors 22 – 24171/172 Diode Array Detectors...........................................2315X Series UV/VIS Detectors .................................................23112 UV Detector ........................................................................24PREPELS™ II Detector .................................................................24

HPLC: Fraction Collectors 25 – 27PREPFC™ Fraction Collector .....................................................26FC 203B/FC 204 Fraction Collectors ..................................27

Control Software 28 – 30TRILUTION® LC Software ..............................................................29TRILUTION® LH Software .............................................................30

GPC Clean-up Systems 31 – 34Automated GX-271 GPC Clean-up System ......................32GX-271 GPC/SPE Clean-up System.....................................33Manual GPC Clean-up System .............................................34

Solid Phase Extraction Systems 35 – 39GX-271 ASPEC™, with 406 Single Syringe Pump ...........36GX-271 ASPEC™, with 406 Dual Syringe Pump ..............36GX-274 ASPEC™, with two 406 Dual Syringe Pumps ...37Quad-Z 215 Liquid Handler for SPE ...................................38SPE 215 System .........................................................................39Code 300 Series Racks ............................................................39

Liquid Handlers 40 – 47GX-281 Liquid Handler ...........................................................41GX-271 Preparative Liquid Handler ...................................42GX-271 Analytical Liquid Handler ......................................43GX-271 Liquid Handler without pump .............................43GX-274 Liquid Handler ...........................................................44 Quad-Z 215 Liquid Handler ..................................................45Quad-Z 215 Liquid Handler with Disposable Tips ........45Multiple Probe 215 Liquid Handler ....................................46215 Liquid Handler ..................................................................46223 Sample Changer ...............................................................47

Accessories 48 – 52VALVEMATE® II Valve Actuator ............................................49Orbital Shaker ............................................................................50RackCount™ System ................................................................50818 AutoMix ...............................................................................51852/853/854 Peltier Racks .....................................................51242/542 Peltier Racks ..............................................................52Racks - Standard and Custom ..............................................52

References 53 – 70Gilson Rack/Instrument Compatibility Chart ....... 53 – 56Gilson Probe Selection Guide .................................... 57 – 60Periodic Table ............................................................................61Flow Cell Selection Chart .......................................................61pH of Common Acids and Bases .........................................62Column Sizes and Capacities ...............................................62Maximum UV Absorbance

for Commonly Used Solvents..........................................63Optimizing Preparative HPLC ..............................................64Separation Scale-up Equations ...........................................64Solvent Miscibility Table ........................................................65Solvent Polarity Chart .............................................................65Common Conversion Tables ...................................... 66 – 67Gilson Applications ........................................................ 68 – 70

Table of Contents

3Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems.

Gilson HPLC Systems

Gilson’s variety of detection solutions off ers excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection. (see pages 22 – 24)

The GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fulfi lling numerous laboratory needs at an economical price. (see pages 7, 8, 14 & 15)

The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models.(see page 16)

Gilson’s VALVEMATE® II Valve Actuator off ers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions.(see page 49)

The GX-281 Liquid Handler is Gilson’s premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology.(see pages 5, 6, & 13)

Gilson’s accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads. (see pages 17 – 21)

Gilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purifi cation needs. Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints. All models accommodate innovative injection and fraction collection capabilities within a single platform. TRILUTION® LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software.


Column ID

DescriptionPage

Number2

mm

4.6

mm

10

mm

20

mm

30

mm

50

mm

10

0 m

m

15

0 m

m

GX-281 Preparative HPLC System

flow rate 1 – 200 mL/mininjection loop volume 250 μL – 25 mL

sample mass 15 g/injection5

GX-281 Analytical to

Semi-preparative HPLC System

flow rate 0.15 – 30 mL/mininjection loop volume 2 μL – 2 mL

sample mass 300 mg/injection6

GX-271 Preparative HPLC System

flow rate 1 – 200 mL/min injection loop volume 250 μL – 25 mL


GX-271 Analytical to

Semi-preparative HPLC System

flow rate 0.15 – 30 mL/mininjection loop volume 2 μL – 5 mL

sample mass 300 mg/injection8

845ZPREP™ Semi-preparative

to Preparative HPLC System



cLC™ Analyticalto Preparative

HPLC System


sample mass 15 g/injectionflash chromatography

9

GX-271 Fraction Trapping

HPLC System

flow rate 25 – 40 mL/mininjection loop volume up to 25 mL

aspiration fl ow rate5 mL/min for fraction loading

10

HPLC: Systems

The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients.(see pages 17 – 21)

Specifications

Sample Loading Capacity

15 g/injectionOptimal Column Size

4.6 – 100 mm

Maximum Injection

Pressure

3,000 psi (207 bar)

Injection Loop Volume

250 μL – 25 mL Flow Rate

1 – 200 mL/minMaximum Pump Pressure

3,040 psi (210 bar)

SSThe GX-281 Preparative HPLC System allows for injection, fraction collection and reinjection. The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers.

GX Direct Injection technology features an injection port that connects directly to the valve, minimizing volume associated with connective tubing.(located behind the racks)

The GX Z-Injection Module allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover.

The GX Prep Solvent System features a dynamic range of volumes and fl ow rates using syringeless technology within a solvent delivery pump capable of accessing fi ve off -bed solvents.


Specifications


300 mg/injectionInjection Loop Volume

2 μL – 2 mL

Optimal Column Size

2 – 20 mm

Flow Rate

H1: 150 μL/min – 15 mL/minH2: 30 μL/min – 30 mL/min

Maximum Injection Pressure

5,000 psi (345 bar)

Maximum Pump Pressure

H1: 8,700 psi (600 bar)H2: 4,350 psi (300 bar)

HPLC: Systems

Specifications


15 g/injectionInjection Loop Volume

250 μL – 25 mL

Optimal Column Size

10 – 100 mmFlow Rate

1 – 200 mL/min


3,000 psi (207 bar)Maximum Pump Pressure

3,040 psi (210 bar)

The GX Prep Solvent System uses syringeless technology and allows access to fi ve off -bed solvents.

A VALVEMATE® II Valve Actuator can be incorporated for multiple column selection or column regeneration applications.(see page 47)

A single, mid-size instrument for injection and fraction collection with separate fl uid pathways to reduce cross-contamination of fractions.

Mid-to-large collection capacity in a small footprint.

The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve.

The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes. Four rinse solvents are available for selection.

Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection. The cycle time of the injection is minimized by eliminating rinse steps which are required with single fl uid path confi gurations.

Binary gradient preparative HPLC pumps provide high-fl ow capabilities.(see pages 17 – 21)

The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection.

Modular detector options available. (see pages 22 – 24)

Detection solutions are available for DAD, fi xed or variable wavelength UV/VIS, UV and ELS detection.(see pages 22 – 24)

The GX Direct Injection Module can utilize both 1/16" and 1/8" injection valves, allowing for a wide variety of sample volumes and loop options.Semi-preparative

HPLC pumps areoff ered to perform binary gradients.(see pages 17 – 21)


HPLC: Systems

Specifications


300 mg/injectionInjection Loop Volume

2 μL – 5 mL

Optimal Column Size

2 – 20 mm

Flow Rate

H1: 150 μL/min – 15 mL/minH2: 30 μL/min – 30 mL/min


5,000 psi (345 bar)


H1: 8,700 psi (600 bar)H2: 4,350 psi (300 bar)

The GX Analytical Solvent System features innovative syringeless technology and access to fi ve off -bed solvents.

The GX-271 is a mid-size liquid handler/fraction collector with a bench-saving footprint featuring separate fluid pathways that reduce cross-contamination of samples and fractions.

For confi rmation or added detection capabilities of non-chromaphoric compounds confi gure an additional ELS detector with a multi-channel DAD detector.(see pages 22 – 24)

Gilson’s 845ZPREP™ System features Gilson’s first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications.

Specifications


15 g/injectionMaximum Injection Pressure

3,000 psi (207 bar)

Optimal Column Size

4.6 – 50 mmMaximum Pump Pressure

3,040 psi (210 bar)


100 μL – 10 mLCarryover

< 0.01%

Injection Variability

< 1.5%Flow Rate

1 – 200 mL/min

SS

Gilson’s cLC™ System is a complete chromatography system capable of automating the purification of compounds using both normal and reversed-phase columns. The cLC System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the cLC System enabling automated on-line evaluation of the collected fractions for purity and recovery.

Specifications


15 g/injectionInjection Loop Volume

5 μL – 25 mL

Optimal Column Size

4 – 100 mmFlow Rate

1 – 200 mL/min


5,000 psi (345 bar)


33X Pump: 3,040 psi 32X Pump: 8,700 psi

SS

Binary gradients are achieved with analytical to semi-preparative HPLC pumps.(see pages 17 – 21)

The low mount collection valve enables fraction collection to tubes and contains two separate liquid paths which reduce cross-contamination.

Mid-to-large bed capacity, accommodating both samples and fractions.


HPLC: Systems

Gilson’s revolutionary Fraction Trapping System is unlike any other system on the market today. The Fraction Trapping System is ideally suited for processing fractions up to 20 mL in volume and concentrations up to 500 mg that have been purified on virtually any preparative HPLC system. Washing steps can be performed for removal of salts, buffers and mobile phase modifiers such as TFA that can hinder further LC/MS analysis. Fractions containing greater than even 50% water content can be processed and collected in 100% of organic solvent. This allows for fast dry down times at lower temperatures for samples that are heat sensitive. Dry down times of 12 – 24 hours can be reduced to less than 30 minutes.

Specifications

Flow Rate

50 mL/minWavelength

190 – 700 nm


up to 25 mLAspiration Flow Rate

5 mL/min for fraction loading

Detector

Variable Dual Wavelength

SS

Trapping Column Specifi cations

Trapping Columns Recommended Load Capacity Recommended Flow Rate

10 mm ID x 150 mm 200 mg 25 mL/min

21.2 mm x 150 mm 500 mg 40 mL/min

GX-271 Preparative

and Analytical Liquid

Handler/Injector

Bed Capacity

TubeDiameter

Sample Quantity

10 mm 540

13 mm 300

15 mm 220

16 mm 220

17 mm 220

18 mm 220

28 mm 70

Note: tubes up to 200 mm tall can be

accommodated

Dual Column with re-generation for increased throughput and solvent selection of up to eight diff erent solvents.

Product #: 21120001(GX-271 Fraction Trapping System)

Most reversed phase fractions contain high water content, salts, modifi ers and buff ers resulting in long dry down times up to 24 hours.

Process fractions in 8 to 12 minutes with volumes up to 20 mL and concentrations up to 500 mg.

Gilson’s Fraction Trapping System completely removes water content, buff ers, modifi ers and salts while reducing dry down from hours to minutes.

Optional breakthrough detector and secondary fraction collector

TRAP – Sequestration from fraction of desired solute(s) by a solid phase

RINSE – Removal of buff ers, modifi ers, salts, etc. by appropriate rinsing of the solid phase and the ‘trapped’ solute(s)

ELUTE – ‘Trapped’ solute(s) desorbed to produce a relatively small volume of ‘dry’ organic solvent solution of the desired solute

Steps involved in Fraction Trapping

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 13

HPLC: Liquid Handlers/Injectors

Gilson’s liquid handlers and injectors offer the broadest range of instrument capabilities on the market today. From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system.

Specifications

Reproducibility

< 1% CV (total loop)< 3.5% (partial loop)

Flow Rate

1 – 200 mL/min


250 μL – 25 mLMaximum Injection Pressure

5,000 psi (345 bar)

Accuracy

98% (100 μL – 25 mL)Injection Carryover

< 0.005%Dimensions (w x d x h)

87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)

Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology.

Product #s: 261030 (GX-281 Liquid Handler)261031 (GX-281 Liquid Handler with bar code)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)261353 (GX Z-Injection Module)

Injection Loop

Volume

Liquid Handling

Accuracy

Injection

Carryover

Page

NumberLiquid

Handler

Fraction

Collection

GX-281 Liquid Handler/ Injector

250 μL – 25 mL 98% (100 μL – 25 mL) < 0.005% 13

GX-271 Preparative Liquid

Handler/Injector250 μL – 25 mL 98% (50 μL – 25 mL) < 0.05% 14

GX-271 Analytical Liquid Handler/Injector

2 μL – 2 mL 98% (10 μL – 25 mL) < 0.05% 15

215 Liquid Handler/Injector

5 μL – 10 mL 99.7% (50 μL) < 0.1% 16

Multiple Probe 215 LiquidHandler/Injector

5 μL – 5 mL 99.2% (500 μL) < 0.05% 16

Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to fi ll.

Two unique and separate liquid pathways for injection and fraction collection provide a zero-contamination environment.

The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance.

GX-281 Liquid Handler/Injector Bed Capacity

Tube Diameter

Sample Quantity

13 mm 576

15 mm 576

16 mm 450

18 mm 420

25 mm 180

28 mm 162

Note: tubes up to 220 mm tall can be accommodated

Injection, collection and analysis

Time (minutes)

(see page 53 for the Gilson Rack/Instrument

Compatibility Chart)

mVo

lts

% M

obile

Pha

se

Frac

tion

Zone

, 1Fr

actio

n Zo

ne, 2

Frac

tion

Zone

, 3Fr

actio

n Zo

ne, 4

Frac

tion

Zone

, 5Fr

actio

n Zo

ne, 6

Frac

tion

Zone

, 7Fr

actio

n Zo

ne, 8

Frac

tion

Zone

, 9Fr

actio

n Zo

ne, 1

0

Frac

tion

Zone

, 11

Frac

tion

Zone

, 12

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 15

Peak RetentionTime (minutes)

Area Count(μVmin/100)

StandardDeviation CV

Theophylline 2.8 14901136.11 44928.68 0.30%

Caffeine 4.2 12267689.70 36503.01 0.30%

Phenol 8.4 13314605.43 73087.21 0.55%


The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23" of linear bench space. Large-bore injection modules feature a continuous flow path design for preparative applications. Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for bio-compatible applications.

Specifications

GX-271 Preparative Liquid Handler/Injector

Flow Rate

1 μL/min – 50 mL/minReproducibility

< 1% CV (total loop)



5,000 psi (345 bar)

Accuracy



59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Specifications

GX-271 Analytical Liquid Handler/Injector

Flow Rate

1 μL/min – 5 mL/min

Reproducibility

< 1% CV (total loop)< 3.5% (partial loop)



5,000 psi (345 bar)

Accuracy



59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Injection Recovery

1 99.86%

2 96.61%

3 98.22%

4 96.64%

5 100.10%

6 98.99%

7 99.57%

8 99.69%

9 99.81%

10 100.36%

Average Recovery 99.99%

Standard Deviation 0.01

CV 1.39%

The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology.

The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance.

Ethernet based communication provides better connectivity.

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler)2614101 (GX-271 Liquid Handler without pump)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)

Separate fraction collection fl ow paths prevent contamination from the injection process.

(see page 53 for the Gilson Rack/Instrument Compatibility Chart)

Available in an analytical or prep model, the solvent systems feature dynamic fl ow rate ranges and can access fi ve off -bed solvents.

(see page 5353 ffo tr thhe GilGilson RRa k/ck/IInsttr ment

GX-271 Preparative

and Analytical Liquid

Handler/Injector

Bed Capacity

TubeDiameter

Sample Quantity

10 mm 540

13 mm 300

15 mm 220

16 mm 220

17 mm 220

18 mm 220

28 mm 70

Note: tubes up to 200 mm tall can be

accommodated

Jet Wash Rinse technology provides fl owing rinse for fast and effi cient rinsing protocols utilizing up to two separate fl owing rinse solvents.

100

80

60

40

20

0

300

250

200

150

100

50

0

-500 1 2 3 4 5

% M

obile

Pha

se

mVo

lts

Time (minutes)

Frac

tion

Zone

, 1

Frac

tion

Zone

, 2

Frac

tion

Zone

, 3

Recovery data: Theophylline (5 mg/mL), Caff eine (5 mg/mL) and Phenol (3 mg/mL) solution with 1 mL injection loop volumes

calculated from reinjection of collected Theophyline fractions (Zone 2)

100

80

60

40

20

0

% M

obile

Pha

se

1250

1000

750

500

250

0

-2500 2 4 6 8 10 12

mVo

lts

Time (minutes)

Theophylline

Caffeine

Phenol

Series of ten 20 μL total loop injections of Theophylline, Caff eine and Phenol

17Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps.

Specifications

Reproducibility

liquid handling: 1.37% CV (50 μL)injection: < 0.9% (1 mL) (total loop)

Accuracy

liquid handling: 99.7% (50 μL)Injection Carryover

< 0.1%


5 μL – 10 mLDimensions (w x d x h)

91 x 61 x 56 cm (36 x 24 x 22 in)

SS

The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform.

Product #s: 2510121 (215 Liquid Handler)251511 (819 Injection Module) 251551 (845Z Injection Module)251552 (845ZPREP™ Injection Module)


Product #: 25101311 (Multiple Probe 215 Liquid Handler)

Inject onto FIA/MS, parallel HPLC and MUX systems using the optional 849 Multiple Injection Module.

HPLC: Pumps

Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations.

This versatile, large bed liquid handler accommodates more than 3,000 racks to hold virtually any sample vessel.

Customize by adding accessories and components to meet laboratory needs.

The 849 Multiple Injection Module allows four simultaneous injections, ideal for parallel HPLC and MUX systems.

Product #: 2515154 (849 Multiple Injection Module)

Flow Rate Pressure

Column ID

Page

Number

1 m

m

2 m

m

4 m

m

10

mm

20

mm

> 5

0 m

m

333/334Prep-scale

HPLC Pumps1 – 200 mL/min

3,040 psi (210 bar)

18

331/332Semi-preparative

HPLC Pumps0.5 – 50 mL/min

8,700 psi (600 bar)

19

321/322Analytical to

Semi-preparativeHPLC Pumps

H1: 0.15 – 15 mL/minH2: 0.3 – 30 mL/min

H1: 8,700 psi (600 bar)

H2: 4,300 psi (300 bar)

19

30X SeriesHPLC Pumps


8,700 psi (600 bar)

20

402Syringe Pumps


110 psi (8 bar) for 0.1 – 10 mL syringes

40 psi (3 bar) for 25 mL syringe

— 20

Minipuls Evolution® Peristaltic

Pump

0.2 μL/min –340 mL/min

42 psi(3 bar)

— 21

Minipuls 3Peristaltic

Pump

standard(1, 2, 4 or 8 channels):

0.3 μL/min – 30 mL/minhigh-fl ow rate

(2 or 4 channels): 1 – 220 mL/min

standard: 72 psi (5 bar)

high-fl ow rate: 42 psi (3 bar)

— 21

The Multiple Probe 215 Liquid Handler/Injector is capable of high-throughput automation of all basic liquid handling tasks such as sample transfer, dilutions, mixing, reagent addition and plate reformatting.

Confi gure with the 0.015" ID analytical valve or the 0.040" ID preparative valve.

Specifications

Reproducibility

liquid handling: 0.57% CV (500 μL)injection: < 2% CV (500 μL) (partial loop)

Accuracy

liquid handling: 99.2% (500 μL)Injection Carryover

< 0.05%


5 μL – 5 mLDimensions (w x d x h)

97.8 x 61 x 55.8 cm (38.5 x 24 x 22 in)

Multiple Injection Module Option

Valve Switching Speed

RV700-100: < 135 msRV700-112: < 370 ms

Dimensions (w x d x h)

61 x 18 x 13 cm (24 x 7 x 5 in)


Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump)38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump)

Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilson’s 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography. Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary).

The entire preparative HPLC system is controlled with TRILUTION® LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation.

Time (minutes)

Prog

ram

med

Com

posi

tion

(% E

)

Det

ecte

d Co

mpo

sitio

n (m

AU)120

100

80

60

40

20

0

300

250

200

150

100

50

00 5 10 15 20 25 30

Gradient Linearity and Accuracy

Time (minutes)

mVo

lts

% M

obile

Pha

se

210

180

150

120

90

60

30

0

-30

100

80

60

40

20

00 2 4 6 8

Retention Time Repeatability

Series of Ten Injections

Methyl PB Ethyl PB Propyl PBAverage Retention

Time 3.44 4.85 6.08

Standard Deviation

0.02 0.02 0.02

CV 0.72% 0.34% 0.29%

Stackable to conserve bench space.

Capable of milligram-to-gram level mass-throughput per injection up to 15 g depending on column size and load capacity.

Selection of up to four solvents per pump for a total of eight solvents in a binary pumping system.

Product #s: 38103211 (321 Pump, H1)381032211 (left) (322 Pump, H1)38103212 (right) (321 Pump, H2)381032221 (322 Pump, H2)

The 321 and 322 multi-solvent pumps are designed for use with Gilson’s HPLC analytical and semi-preparative chromatography systems.

Specifications

Flow Rate

0.5 – 50 mL/min (8,700 psi)Accuracy

± 2% or 13 μL/min

Reproducibility

H2: ± 0.7% or 7 μL/min


331: 26 x 41 x 51 cm (10 x 16 x 20 in)332: 26 x 41 x 39 cm (10 x 16 x 15 in)

Gilson’s 331 and 332 dual-piston pumps for semi-preparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance.

Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump)38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump)

Smooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with high-effi ciency stationary phases.

Milligram-level sample mass-throughput per injection; 150 mg with a 20 mm bore column.

Solvent selection on each pump with optional 4-solvent valve for up to eight solvents.

High pressure Adjustable Volume Dynamic Mixer (AVDM™) enables fast gradient response times and homogeneous mixtures for analytical to semi-preparative applications.

While the pumps are controlled via TRILUTION® LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control.

A single 321 or 322 Pump is capable of binary gradients.

H1 pump heads are ideal for 2 to 10 mm ID columns.

Specifications

Flow Rate

H1: 0.15 – 15 mL/min (8,700 psi)H2: 0.3 – 30 mL/min (4,300 psi)

Accuracy

± 2% or 13 μL/min

Reproducibility

H1: < 0.7% or 2 μL/minH2: 4 μL/min


321: 26 x 41 x 51 cm (10 x 16 x 20 in)322: 26 x 41 x 39 cm (10 x 16 x 15 in)

The fl ow rate can be doubled with additional parallel pumps to 400 mL/min.

TRILUTION® LC Software provides control within a complete semi-preparative system including data processing and result reporting or stand-alone control via the keypad incorporated on the 331 Pump.

HPLC: Pumps

H2 pump heads are ideal for 4 to 20 mm ID columns.

Specifications

Flow Rate

1 – 200 mL/min (3,040 psi)Accuracy

± 1% or 5.3 μL/min

Reproducibility

< 0.5% or 2.7 μL/min RSD


333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in)


HPLC: Pumps

Specifications

Flow Rate

305/306: 10 μL/min – 200 mL/min307: 10 μL/min – 10 mL/min

Accuracy

< ± 1%

Reproducibility

< 1%


33 x 33 x 15 cm (13 x 13 x 6 in)

SS

Product #s: 360261 (305 Pump)3602661 (right) (306 Pump)3602671 (left) (307 Pump)

Together with Gilson’s manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC.

Tools are not required for pump head removal, allowing accessibility for maintenance. Piston seals, check valves and other parts can be replaced in a matter of minutes.

Specifications

Flow Rate

standard: 0.3 μL/min – 30 mL/min (72 psi, 5 bar)

high-fl ow: 1 – 220 mL/min (42 psi, 3 bar)

Head Speed

continuous adjustment from 0 – 48 rpm by 0.01 rpm increments up to 9.99 rpm,

0.1 rpm increments above 10 rpm

Tubing ID

standard: 0.25 – 4 mmhigh-fl ow: 2 – 8 mm


15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in)

The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device.

Product #: F155004 (Minipuls 3 Pump, single channel)

The 305 and 307 Pumps are programmable through the incorporated keypad.

The 307 model functions as an isocratic pump with a built-in manometric module.

Dynamic mixers ensure effi cient, dependable gradient creation.

Product #s: F410511 (left) (402 Single Syringe Pump)F410512 (right) (402 Dual with Tee Syringe Pump)F410513 (402 Dual Syringe Pump)

The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more. They offer speed and reliability for repetitive liquid handling tasks.

402 Single, ideal for sampling injectors.

Available in three configurations:

402 Dual with Tee, provides rapid dilutions in both small and large volumes.

402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents.

Specifications

Reproducibility

0.8% (10 μL)Flow Rate


Accuracy

98.2% (10 μL)Dimensions (w x d x h)

17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in)

An ideal addition to Gilson liquid handlers and injectors including the GX and 215 Series instruments.

The Minipuls 3 accommodates wide fl ow rate ranges using either the standard or high-fl ow pump head. Pump heads are available with one, two, four or eight channels.

Multiple stainless steel rollers drive the pump to produce smoother, low pulse fl ows.

The 306 model functions as a slave unit, controlled by either a

305 or TRILUTION® LC Software.

Specifications

Reproducibility

≤ 0.1% on all channels

Tubing ID

standard: 0.25 – 4 mmhigh-fl ow: 2 – 8 mm

Flow Rate

standard: 0.2 μL/min – 70 mL/min (42 psi, 3 bar)high-fl ow: 1 – 340 mL/min (29 psi, 2 bar)

Head Speed

continuous adjustment from 0 – 60 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm


Module: 164 x 182 x 169 mmMF1 pump head: 164 x 56 x 169 mmMF4 pump head: 164 x 92 x 169 mmHF2 pump head: 164 x 92 x 169 mm

SS

Offering unmatched flexibility and performance for liquid transfer applications, the Minipuls Evolution® is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations.

Optimized six roller drive limits pulsation.

Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal.

Product #: F110701

(Minipuls Evolution® Pump)

Enhanced reliability and ease of operation are possible because the pump’s speed stability is maintained independent of normal temperature variations from 0˚ to 40˚C. This makes the pump ideal for biological perfusion applications.

(Note: not intended for direct connection to human subjects.)

23Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Detectors.

HPLC: Detectors

Diode Array

(DAD)

Ultraviolet

(UV)

Visible

(VIS)

Evaporative

Light Scattering

(ELS)

WavelengthSpectral

Band WidthChannels

Page

Number

171/172Diode Array

Detectors

190 – 720 or190 – 610 nm

— 8 23

15X Series UV/VIS

Detectors190 – 700 nm 9 nm

dual & single

23

112 UV Detector

254 or 280 nm

0.2 or12 nm

single 24

PREPELS™ II Detector

— — single 24

Time (minutes)

mVo

lts

% M

oblie

Pha

se

120

100

80

60

40

20

0

-20

100

80

60

40

20

00 2 4 6

Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications.

Gilson’s 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC. Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design.

Product #s: 18100001 (171 Diode Array Detector)18100002 (172 Diode Array Detector)

With a remote fl ow cell, the power of the detector is located directly at the outlet of the column, which yields virtually no peak broadening.

An interchangeable fl ow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications.

Specifications

Pixels171: 256172: 512

S/N Ratio171: < 3 x 10-04 AU172: < 2 x 10-04 AU

Wavelength171: 190 – 720 nm172: 190 – 610 nm

Pixel Distance171: ~2.2 nm/pixel172: ~0.8 nm/pixel

Wavelength Accuracy< 0.5 nm

Wavelength Repeatability< 0.1 nm

Baseline Drift5 x 10-04 AU/h

Wavelength Temperature Drift< 0.005 nm/°C

Dimensions (w x d x h)26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)

Gilson’s 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications.(see page 61 for Flow Cell Selection Chart)

Product #s: 10105311 (right) (151 UV/VIS Detector, with fl ow cell/accessory kit)10105411 (152 UV/VIS Detector, with fl ow cell/accessory kit)10105511 (155 UV/VIS Detector, with fl ow cell/accessory kit)10105611 (left) (156 UV/VIS Detector, with fl ow cell/accessory kit)

Specifications

Lamp

UV: deuterium, 750 hrsVIS: tungsten/halogen, 500 hrs

Setting Accuracy

± 2 nm

Wavelength

190 – 700 nmSensitivity

0.001 – 2 AUSetting Precision

± 0.2 nm

Drift

3 x 10 – 4 AU/hrSpectral Band Width

9 nmDimensions (w x d x h)

26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)

The following modes are available to specify detection conditions and basic operating parameters: – Single wavelength with dual sensitivities (151 and 152) – Dual wavelengths (155 and 156) – Scan wavelengths (155 and 156)

Choose either Gilson’s TRILUTION® LC Software or the stand-alone keypad control on the 151 and 155 Detectors.

Up to ten methods can be stored.

25Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors.

HPLC: Detectors

HPLC: Fraction Collectors

Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between.

CapacityFlow Rate Microplate Tubes 1 L Bottles

Page

NumberLarge Small

PREPFC™Fraction

Collector200 mL/min 26

FC 204Fraction

Collector

20 or 200 mL/min

27

FC 203BFraction

Collector20 mL/min 27

The 112 UV Detector is a rugged, economical detector for analytical, preparative HPLC and GPC clean-up.

Product #: 0310511

(112 UV Detector, with lamp and 11 μL fl ow cell/accessory kit)

Specifications

Lamp

mercury (standard): 254 nm, 2,000 hrsphosphor-coated mercury: 280 nm, 500 hrs

Spectral Band Width

254 nm: 0.2280 nm: 12

Flow Cell Sensitivity

< 4 x 10 – 4 AU for fl ow step of 0.5 – 2 mL/min (Methanol)

Drift

3 x 10 – 4 AU/hrWavelength

254 or 280 nmDimensions (w x d x h)

32 x 32 x 13 cm (12.6 x 12.6 x 5.2 in)

SS

Accessible lamp/fi lter assemblies for selecting wavelengths.

Fixed wavelength design for increased sensitivity and durability.

10 mm pathlength analytical fl ow cell provides excellent sensitivity ranging from 0.001 to 1 AUFS (Absorbance Units, Full Scale).

Reduced operating and setup time with a single step baseline function.

Event-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system.

A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample.

Gilson’s evaporative light scattering (ELS) detector, PREPELS™ II, provides capabilities on the analytical-scale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape.

Product #s:

18100100 (PREPELS™ II Detector, 120V)18100200 (PREPELS™ II Detector, 240V)

Time (minutes)

ELSDUV

mVo

lts

% M

oblie

Pha

se

150

125

100

75

50

25

0

100

80

60

40

20

00 2 4 6

Specifications

Light Source

670 nm laser diode, < 5 mWEvaporative Zone Temperature

ambient – 120˚C

Gas Requirement

65 ± 5 psi Nitrogen or Argon

Liquid Flow Rate

0.2 – 5 mL/minSplitting packages available to accommodate

system fl ow rates up to 75 mL/min

Nebulization Temperature

0˚ – 70˚CDimensions

25 x 46 x 29 cm (9.8 x 18 x 11.5 in)

SS

The PREPELS II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides.

27Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors.

Gilson’s PREPFC™ Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications. The PREPFC features an adjustable tray that accommodates various vessel heights, volumes and sizes.

Pocket PC control for stand-alone operation allows the PREPFC to be added to any HPLC system capable of sending contact closures.

Specifications


50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in)

Valve Dead Volume

114 μL internal volume; 6 μL dead volume from common port to normally closed port

Liquid Contact Material

PTFE and 316 stainless steelFlow Rate

200 mL/min


< 20 msCollection Modes

slope, level, time or volume

SS

High-fl ow fraction collection valve.

Product #s: 17110101 (PREPFC™ Fraction Collector - Ethernet)171071 (PREPFC™ Fraction Collector)

PREPFC™ Control Software:• Remote start and tube advance• Up to ten programmable collection windows• Active chromatogram display with start and

stop fraction collection lines• Customizable collection patterns and

rack addition

Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes. Collect fractions by time, drop or peak, accommodating all LC applications. Both models accept a wide variety of collection vessels including tubes, vials and microplates.

Product #s: 171011 (right) (FC 203B)171011DV (FC 203B, 3-way valve)171041 (left) (FC 204)171043 (FC 204, prep valve)

Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the column’s void volume, areas of non-interest and equilibration volumes.

The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B).

In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specifi ed mV threshold.

The fraction collector utilizes multi-column adapters for collecting up to 18 separate channels.

Its compact size fi ts in most fume hoods.

Contact closure inputs are available to remotely start, advance or stop fraction collection.

Specifications


FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in)FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in)


100 ms

Liquid Contact Material

PTFE and 316 stainless steel

Max Flow Rate

FC 203B: 20 mL/minFC 204: 20 or 200 mL/min

Valve Dead Volume

FC 203B/FC 204: 64 μL internal volume; 3.5 μL dead volume from common port to normally closed portFC 204: 114 μL internal volume; 6 μL dead volume from common port to normally closed port

SS

Increased X/Y speed optimizes fraction collection.

300.0

250.0

200.0

150v

100.0

50.0

0.0

-50.00.0 1.0 2.0

mVo

lts

Minutes

HPLC: Fraction Collectors

300.0

250.0

200.0

150v

100.0

50.0

0.0

-50.00.0 1.0 2.0

mVo

lts

Minutes

PREPFC Bed Capacity

Sample

QuantitySize Vessel Rack

320 13 x 100 mm (9 mL) tubes (4) Code 343 Racks




54 28 x 57 mm (25 mL) scintillation vials (2) Code 204 Racks

54 30 x 115 mm (50 mL) conical bottom tubes

(2) Code 222 Racks

18 48 x 113 mm (125 mL) bottles (2) Code 211 Racks

11 (500 mL) bottles (1) Code 91 Rack

8 (1 L) bottle (1) Code 92 Rack

240 12 x 32 mm (2 mL) vials (4) Code 330 Racks

56 (20 mL) scintillation vials (4) Code 333 Racks




Stand-alone instruments are controlled with an incorporated keypad or through Gilson’s TRILUTION® LC Software.

Increase bed capacity by confi guring up to four fraction collectors in a single system (provides over 1,000 fractions).


29Visit www.gilson.com or call 608-836-1551 for more details on Gilson Control Software.

Control Software

Gilson offers powerful software to complement robust instruments. With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilson’s software packages allow effortless yet complete control of instruments focusing on the science rather than the software.

Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries.

Gilson has redefined purification with the HPLC system control software, TRILUTION® LC. This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require.

Fraction Collection Capabilities:

• Collection modes: slope, level, time or volume

• Conditional logic fraction collection

• Graphical fraction collection optimization

• Slope collection settings: front, tail and APEX

• Auto sample list generation

Open Access Module:

• Administrator control and permission-setting capabilities

• Walk-up user access

• Minimal training required

Conforms to the application needs rather than compromising the application in order to fi t the software.

Confi guration and Virtual Lab Screen

Computer Recommendation

Processor

Intel® Pentium® 4 (2.8 GHz);

Intel® Core™ 2 Duo (1.86 GHz)

Fixed Drive

120 GB, available space: 4 GB

(6 GB at install)

Video Card

1280 x 1024 with24 bit True Color

RAM

2 GBAdditional Drive

DVD/CD-RW

I/O Ports

1-Serial port1-Ethernet port

Operating System

Microsoft® Windows® XP Professional SP2 or SP3

HPLC Systems SPE SystemsGPC Clean-up

SystemsLiquid Handlers

Page

Number

TRILUTION® LC 29

TRILUTION® LH 30

Application Run ScreenAA li i R S

Product #s: 210631R21 (TRILUTION® LC Media)21063130 (TRILUTION® LC Lifetime License)21063130L5 (TRILUTION® LC Multi-user Lifetime License - 5)21063130L10 (TRILUTION® LC Multi-user Lifetime License - 10)21063131 (TRILUTION® LC 1 year License)Note: Requires Oracle® Database 10g Express Edition

Method Builder Control Screen

Fraction Collection Settings Page

31Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems.

Main Menu

SS Dispense Task Property Page

Method in Method Builder Screen

Simulation Mode in Application Run Screen

Control Software

TRILUTION® LH Software, designed by scientists for scientists, is Gilson’s complete control solution for liquid handling and solid phase extraction applications. TRILUTION LH features a user-friendly graphical interface with icon-based drag-and-drop functionality minimizing the learning curve and optimizing application performance.

Over 70 Gilson-supplied tasks enable quick and effi cient method programming.

Quick and eff ortless instrument setup, bed layout and method creation.

Sample list with spreadsheet functionality and graphical run simulation.

Run methods in either batch or sequential mode to optimize performance.

Manual control for performing routine tasks such as priming instruments and troubleshooting.

Graphical custom task creation and cartridge monitoring.

Product #s: 210630R20 (TRILUTION® LH Media)21063020 (TRILUTION® LH Lifetime License)21063021 (TRILUTION® LH 1 Year License) Note: Requires Oracle® Database 10g Express Edition

Computer Recommendation

Processor

Intel® Pentium® 4 (2.8 GHz)

Fixed Drive

80 GB, available space: 4 GB

(6 GB at install)

Video Card

1280 x 1024 with 24 bit True Color

RAM

1 GBAdditional Drive

DVD/CD-RWSerial Ports

2

Operating System

Microsoft® Windows® XP Professional SP2 or laterMicrosoft® Windows® 2000 SP4

CC

Liquid Disposable Solid Phase Solvent Tweaks Auxiliary Handling Tips Extraction System

Gilson’s gel permeation chromatography (GPC) clean-up systems are an ideal solution for post-extraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime.

GPC Clean-up Systems

Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements.

Fraction

CollectionMultiple Fractions

Solid Phase Extraction

ControlPage

Number

Automated GX-271 GPC

Clean-upSystem

0.25 – 450 mL (optional)

TRILUTION® LC Software 32

GX-271 GPC/SPE Clean-up

System

0.25 – 450 mL(optional)

TRILUTION® Software 33

Manual GPC Clean-up

System0.25 – < 1000 mL

(optional)

307 Pumpkeypad

34

33Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems.


Specifications

Automated GPC Systems

Flow Rate

0.01 – 10 mL/min


20 μL – 5 mL

Wavelength

254 or 280 nm

Maximum Pressure

4,000 psi (276 bar)

Compatible with various GPC columns from low pressure glass to high pressure stainless steel.

Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector)21110001 (Automated GX-271 GPC Clean-up System)

The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels.

Automated GPC Systems Bed Capacity

SampleQuantity

DescriptionGPC

Collection RackProduct #

36 38 x 200 mm (170 mL) collection tubes Code 320 Rack 26044005085 25 x 200 mm (70 mL) collection tubes Code 321 Rack 26044005113 61 x 137 mm (250 mL) Boston Round bottles Code 322 Rack 2604400528 Labconco RapidVap (450 mL) fl at-bottom evaporation fl asks Code 323 Rack 260440053

20 Caliper TurboVap II (200 mL) evaporation tubes Code 324 Rack 2604400548 Labconco RapidVap (450 mL) evaporation fl asks, with stem Code 325 Rack 260440055

50 Caliper TurboVap II (50 mL) evaporation tubes Code 326 Rack 26044005632 Labconco RapidVap (100 mL) fl at-bottom evaporation fl asks Code 327 Rack 26044005723 49 x 114 mm (125 mL) Boston Round bottles Code 328 Rack 26044005812 Pyrex 4100 (125 mL) fl at-bottom fl asks for rotary evaporators Code 329 Rack 2604400598 Pyrex (250 mL) fl at-bottom fl asks for rotary evaporators Code 331 Rack 260440060

SampleQuantity

DescriptionGPC

Sample RackProduct #

80 13 x 100 mm (8 mL) tubes Code 343 Rack 26044002544 16 x 100 mm (12 mL) tubes Code 346 Rack 260440049

Gilson’s newly released Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system.

Positive Pressure elution increases reproducibility and precision.

Sample Capacity

SPE: 60 (3 mL), 45 (6 mL)GPC: 80

Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute.

The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overfi ll the sample loop.

35Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems.

Cartridge Sizes

Liquid Handler

Multiple Collection

Positive Pressure Elution

1 mL 3 mL 6 mL MicroplatePage

Number

GX-271 ASPEC™

36

GX-274 ASPEC™

37

Quad-Z 215 Liquid

Handler for SPE

38

SPE 215System

39

Code 300 SeriesRacks

39

Solid Phase Extraction Systems

Automate solid phase extraction (SPE) processes with Gilson’s complete instrument line. From 96-well extraction plates to 1, 3 or 6 mL cartridges, Gilson automated SPE solutions meet any application need.

Analyte Interferences

Conditioning Loading Sample Washing Eluting


Specifications

Flow Rate

0.01 – 10 mL/minWavelength

254 or 280 nm

Maximum Fractions

10 separateMaximum Pressure

4,000 psi (276 bar)


20 μL – 5 mL

Collection of multiple fractions from the same sample extract is done with the VALVEMATE® II Valve Actuator.

Integrate a 112 UV Detector for EPA method compatibility.

Product #s: 21110002

(Manual GPC Clean-up System,with 112 UV Detector)21110003 (Manual GPC Clean-up System)

Keypad provides simple setup and control of the system for time-based fraction collection.

Manual injection valve coordinates injection time.

Gilson’s Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space.



Mobile Rack technology enables the entire SPE process to be automated from the initial condition to the fi nal elution and transfer while allowing access to cartridges and compounds of interest. The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated.

On-bed transfer ports allow access to two additional large volume solvent reservoirs; incorporating a VALVEMATE® II provides access to ten off -bed solvents.

Process four SPE cartridges simultaneously for increased throughput.

Built-in pressure sensing on each fl uid line monitors cartridge pressure and responds to blockage by activating an error handling method.

Product #: 2614010

(GX-274 ASPEC™, with two 406 Dual Syringe Pumps)

Positive Pressure elution increases reproducibility and precision due to process consistency.

The GX-274 ASPEC™ is an ideal solution for mid to high-throughput sample clean-up and extraction.

Specifications

GX-27X ASPEC™ Series

Cartridge Capacity

108 (1 mL), 60 (3 mL), 45 (6 mL)Positive Pressure Modes

syringe or gas cylinder/source

Accuracy

98% (100 μL – 25 mL)Dimensions (w x d x h)

59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Choose between the 406 Single or, for more fl exibility, the 406 Dual Syringe Pump for both large and small volume capabilities. Both pumps have built-in pressure sensing capabilities for detecting clogged fl uid lines and monitoring cartridge pressure on each fl uid line.

The GX ASPEC™ Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 mL cartridges.

The GX-271 ASPEC™ is truly the solid solution for low to mid-throughput sample clean-up and extraction.

Product #s: 2614007 (left) (GX-271 ASPEC™, with 406 Single Syringe Pump)2614008 (right) (GX-271 ASPEC™, with 406 Dual Syringe Pump)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)

Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments.

On-bed access to four 500 mL or 700 mL reservoirs.

Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and fl exibility while performing both SPE and liquid handling.

A utomated S olid P hase E xtraction C artridges

Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up.

Optional safetyshield available.


Code 300 Series Rack Capacity

Sample Quantity

SPE Cartridge

Collection Vessel Rack Product #

96 1 mL tabless 96-well microplate, deep Code 300 Rack 2504300

96 1 mL tabless 96-well microplate, shallow Code 301 Rack 2504301

48 1 mL 12 x 32 mm (2 mL) vials Code 302 Rack 2504302


48 3 mL 12 x 75 mm (5 mL) tubes Code 304 Rack 2504304


20 6 mL 12 x 75 mm (5 mL) tubes Code 306 Rack 2504306



The SPE 215 System provides an automated solution for sample preparation, sample reconstitution and parallel injection onto HPLC or LC/MS systems. Additionally, the SPE 215 enables parallel clean-up, filtration and purification of combinatorial libraries and biological samples.

Specifications

Reproducibility

0.98% (100 μL)Accuracy

97.1% (100 μL)


97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in)

Product #: 25101322 (SPE 215 System)

Positive Pressure elution is achieved with Gilson’s integrated Sealing Foot design.

Mobile Racks automate entire SPE and liquid handling processes.

The SPE 215 accommodates various racks off ering sample preparation solutions for virtually any application.

Up to eight samples can be processed every fi ve minutes or 96 samples in an hour; a solution ideal for high-throughput sample clean-up applications.

Gilson’s Code 300 Series Racks provide the ability to add SPE and filtration to Gilson liquid handlers including the 215 and GX Series instruments.

Sealing Cap technology enables Positive Pressure SPE ensuring better reproducibility and higher precision.

Mobile Rack technology enables automated switching from drain and collect positions to accomplish the entire

SPE process from condition to load, wash and elute, including selective evaluation of individual steps for

method development and optimization available through N+1 movement into individual collection tubes.

Gilson’s Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE.

This liquid handler accommodates various racks maximizing flexibility for high-throughput applications.

Specifications

Cartridge Capacity

288 (1 mL), 144 (3 mL), 60 (6 mL)Accuracy

98% (100 μL – 25 mL)


91 x 61 x 61 cm (36 x 24 x 24 in)

Using Code 300 Series Racks with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision.(see Code 300 Series Rack Capacity chart on page 39)

Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE )

Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes.


41Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers.

GX-281 Liquid Handler Bed Capacity

Tube Diameter

Sample Quantity

13 mm 576

15 mm 576

16 mm 450

18 mm 420

25 mm 180

28 mm 162

Note: tubes up to 220 mm can be accommodated

25 mL Transfers at 25 mL/min.

Transfers

Volu

me

Del

iver

ed

25

20

15

10

5

0 0 2 4 6 8 10 12

10 mL Transfers at 25 mL/min.

Mea

sure

d Vo

lum

e

10

7.5

5

2.5

0

Dispenses0 20 40 60 80 100 120 140 160 180

5 μL Dispense

The GX-281 Liquid Handler is Gilson’s premier liquid handling instrument. The GX-281 offers unmatched versatility and performance for liquid handling operations including tube-to-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more. The efficient speed of the GX-281 allows applications to be completed quickly and reliably. The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology.

Direct request aspirating and dispensing eliminates the time spent waiting for syringes to fi ll.

Optional integrated bar code scanner verifi es rack types and positions.

Access fi ve off -bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols.

Product #s: 261030 (GX-281 Liquid Handler)261031 (GX-281 Liquid Handler, with bar code scanner)

Aliquot Dilution Mixing

Liquid Handlers

Multiple

Probes

Disposable

TipsReproducibility Accuracy

Page

Number

GX-281Liquid

Handler< 1% (100 μL +) 98% (100 μL – 25 mL) 41

GX-271Liquid

Handler< 1% (100 μL – 25 mL)

98% (50 μL – 25 mL)98% (10 μL – 5 mL)

42

GX-274 Liquid

Handler0.8% (10 μL) 98.2% (10 μL) 44

Quad-Z215 Liquid

Handler2.1% (1 μL of DMSO) 104% (1 μL of DMSO) 45

Quad-Z 215 Liquid Handler with

Disposable Tips

4.3% (1 μL of DMSO) 113% (1 μL of DMSO) 45

Multiple Probe 215 Liquid

Handler0.57% (500 μL) 99.2% (500 μL) 46

215 LiquidHandler

1.37% (50 μL of DMSO) 99.7% (50 μL of DMSO) 46

223 SampleChanger

0.8% (10 μL) 98.2% (10 μL) 47

Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price. Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilson’s liquid handling solutions meet all needs.

(see page 53 for the Gilson Rack/InstrumentCompatibility Chart)

Specifications

Reproducibility

< 1% (100 μL +)Accuracy

98% (100 μL – 25 mL)


87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)Flow Rate



Liquid Level Detection allows for detecting a variety of solvents as low as 20 μL in a 384-well microplate.

Gilson’s GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and fl ow rates and can access fi ve off -bed solvents.

Specifications

GX-271 Analytical Liquid Handler

Accuracy

98% (10 μL – 5 mL)Flow Rate

1 μL – 5 mL/min


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

GX Analytical Solvent System Transfers

Volume CV Accuracy

1 μL 11.98% ± 300 nL

10 μL 4.5% ± 1 μL

1 mL 0.18% ± 20 μL

5 mL 0.2% ± 100 μL

GX Prep Solvent System Transfers

Volume CV Accuracy

50 μL 1.25% ± 2 μL

100 μL 1.18% ± 4 μL

500 μL 0.48% ± 7 μL

1 mL 0.14% ± 5 μL

5 mL 0.09% ± 16 μL

10 mL 0.07% ± 105 μL

25 mL 0.02% ± 46 μL

Product #s: 2614107 (GX-271 Preparative Liquid Handler)2614108 (GX-271 Analytical Liquid Handler)2614101 (GX-271 Liquid Handler without pump)

GX-271 Preparative and

Analytical Liquid Handler

Bed Capacity

Tube Diameter

Sample Quantity

10 mm 540

13 mm 300

15 mm 220

16 mm 220

17 mm 220

18 mm 220

28 mm 70

Note: tubes up to 200 mm can be accommodated

The GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be confi gured with a 402 Syringe Pump.

Consumes only 23" of linear bench space.

Flexible bed accommodates fi ve racks and virtually any tube, vial or microplate.

Ethernet based communication provides better connectivity.

Jet Wash Rinse technology provides fl owing rinse for fast and effi cient rinsing protocols utilizing up to two separate fl owing rinse solvents.

Liquid Handlers

GX Prep Solvent System Transfers

% CV

Volume

1.4

1.2

1

0.8

0.6

0.4

0.2

0

50 μL

100 μL

500 μL

1 mL

5 mL

10 mL

25 mL

14

12

10

8

6

4

2

0

% CV

GX Analytical Solvent System Transfers

Volume

1 μL

10 μL

1 mL

5 mL

The GX-271 Liquid Handler off ers a medium capacity with a small footprint. The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions. Their modular design allows for future expansion by adding injection and fraction collection capabilities.(see GX-271 Liquid Handler/Injector page 14)

Specifications

GX-271 Preparative Liquid Handler

Accuracy

98% (50 μL – 25 mL)Flow Rate

1 μL – 50 mL/min


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

(see page 53 for the Gilson Rack/InstrumentCompatibility Chart)


Liquid Handlers

The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler. This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates.

Product #s: 25101413 (Quad-Z Liquid Handler)2506163 (Quad-Z 215 Adapter Kit for Disposable Tips)2515154 (849 Multiple Injection Module)F410513 (402 Dual Syringe Pump)

Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 μL to 25 mL.

For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm.

Flexible confi guration enables use of fi xed probes and 10 μL, 200 μL and 1 mL disposable tips.

The GX-274 Liquid Handler is a mid-throughput, four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications. The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications.

The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution® or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps.

Specifications

Accuracy

98.2% (10 μL)Reproducibility

< 0.8% (10 μL +)


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Access four on-bed solvent reservoirswithout consuming a rack position.

Product #s: 2614102 (GX-274 Liquid Handler)F410513 (402 Dual Syringe Pump)

Flexible rack system accommodates tubes, vials and microplates.

Optional fl owing rinse station with solvent ports provides active probe rinsing from off -bed solvents.

A VALVEMATE® II can be incorporated enabling access to ten additional off -bed solvents.

Gilson’s Quad-Z 215 Liquid Handler can be configured with a Disposable Tips option, a requirement in molecular biology and biochemistry applications. Disposable tips prevent cross-contamination, which saves time by eliminating rinsing steps. This system includes on-bed storage of tips, as well as a chute for collection of used tips.

Specifications


91 x 61 x 61 cm (36 x 24 x 24 in)

Accuracy

104% (1 μL of DMSO)

Reproducibility

liquid handling: 2.1% (1 μL of DMSO)injection: < 2.0% (500 μL syringe, partial loop);

< 5.0% (500 μL syringe, total loop)

Injection Carryover

< 0.05%Injection Loop Volume

5 μL – 5 mL

Disposable Tips Option

Accuracy

113% (1 μL of DMSO)Reproducibility

0.8% (10 μL +); 4.3% (1 μL of DMSO)


The 223 Sample Changer is a programmable sampler for automated sample preparation and transfer. It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers.

The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP).

Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing.

Product #: 191015 (223 Sample Changer)

Optional fl ow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination.

Accommodates a wide variety of probes as well as standard and custom racks.(see page 53 for the Gilson Rack/

Instrument Compatibility Chart)

Liquid Handlers

Specifications

Reproducibility

0.8% (10 μL)Accuracy

98.2% (10 μL)


53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in)

Choose between an optional 402 Syringe Pump, Minipuls Evolution® or Minipuls 3 Peristaltic Pump to add aspirating and dispensing fl exibility.

Specifications

Reproducibility

0.57% (500 μL)

Carryover

< 1 ppbAccuracy

99.2% (500 μL)


97.8 x 61 x 56 cm (36 x 24 x 22 in)

Specifications

Reproducibility

1.37% (50 μL)

Carryover

< 1 ppbAccuracy

99.7% (50 μL)


91 x 61 x 56 cm (36 x 24 x 22 in)

Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control.

Product #s: 2510121 (215 Liquid Handler)2510191 (215 Liquid Handler without pump)

Select either a four or eight probe confi guration depending on application and throughput requirements.

Product #: 25101311 (Multiple Probe 215 Liquid Handler)

Ideal solution for plate-to-plate transfers.

The Multiple Probe 215 is a high-throughput automated liquid handler capable of tasks such as sample transfers, dilutions, mixing, reagent addition and microplate reformatting. The Multiple Probe 215 features patented Dual Rail Syringe Drive technology, accurate use of four or eight syringes and accommodates probes that fi t all application requirements.

The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more. The 215 Liquid Handler has a punch strength capable of piercing thick or thin fi lm septa and microplate mats that is distinguished throughout the liquid handling industry.

49Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories.

High Pressure Valves:

• Analytical 2-position, 6-port, stainless steel (0.016" ID ports)

• Analytical 2-position, 10-port, stainless steel (0.016" ID ports)

• Preparative 2-position, 6-port, stainless steel (0.030" ID ports)

• Preparative 2-position, 10-port, stainless steel (0.030" ID ports)

• Preparative 2-position, 6-port, PPS (0.030" ID ports)

• Preparative 2-position, 10-port, PPS (0.030" ID ports)

• Multi-position, 6-port, stainless steel (0.016" ID ports)


• Multi-position, 6-port, PEEK (0.016" ID ports)


• Multi-position, 10-port, PEEK (0.016" ID ports)

Product #: 331052AB (VALVEMATE® II Valve Actuator)

The VALVEMATE® II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life. It is designed to facilitate both solvent selection and column switching. The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations.

Specifications


8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in)


< 180 ms port-to-port (depending on valve)

SS

Contact closure control forhome and switch valve position.

Multi-directional keypad control with valve position LED display.

Fast valve switching times minimize pressure spikes and carryover.

Low Pressure Valves:

• Multi-position, 6-port, PPS (0.030" ID ports)




Accessories

Switching Valve Mixing Racks Heating/CoolingPage

Number

VALVEMATE® II Valve Actuator

49

Orbital Shaker 50

RackCount™System

50

818 AutoMix 51

852/853/854Peltier Racks

51

242/542 Peltier Racks

52

40ºC

4ºC

40ºC

4ºC

Complementing robust and flexible instruments, Gilson’s accessories optimize instrumentation to fit application requirements for the ultimate solution.

6 Column Plumbing Schematic

51Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories.

Designed for Gilson’s 215 Series instruments, the 818 AutoMix provides fully automated, software-driven mixing and sampling. Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more.

Gilson offers flexible, accurate and programmable racks for heating and cooling valuable samples; cooling to reduce evaporation and maintain sample integrity, heating to facilitate reactions and improve kinetics.

Product #: 251520 (818 AutoMix)

Gilson offers a variety of standard and custom rack configurations.

Product #s: 2514852 (far left) (852 Peltier Rack)2514853 (center left) (853 Peltier Rack)2514854 (center right) (854 Peltier Rack)2515850 (far right) (Peltier Controller)

Mix an entire rack of samples with oscillating mixing.

Up to fi ve racks can be controlled from a single programmable Peltier controller integrated with a system or as a stand-alone unit.

Precise temperature control from 4 to 40°C.

Accessories

Specifications

Mixing Speed/Accuracy

10 rpm: ± 0.5 rpm20 rpm: ± 1 rpm

Rack Capacity

(1) Code 200 Series Rack


22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in)

Specifications

Rack Capacity

852: (2) 96-well microplates, shallow853: (96) 12 x 32 mm (2 mL) fl at-bottom vials

854: (2) 96-well microplates, deep


11.9 x 33.8 x 16.8 cm (4.7 x 13.3 x 6.6 in)

Gilson’s RackCount™ System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler.

The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing. It is a fully functional, stand-alone instrument equipped with a single-stepper motor.

The Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits.

Specifications

Circular Rotational Speed

20 – 720 rpm

Rack Capacity

(1) Code 200 Series Rack


11.8 x 31.6 x 4.7 cm (4.65 x 12.44 x 1.85 in)

RackCount overlays can be used with new rack purchases or added to existing Gilson Code 200 Series Racks.

They have sequentially numbered fraction collection positions for fi ve complete rack positions and one position for sample locations.

The numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents.

Product #: 251711 (Orbital Shaker)

53Visit www.gilson.com or call 608-836-1551 for more details on Gilson Instruments and Software.

Accessories

Gilson Rack/Instrument Compatibility Chart

Ra

ck C

od

e

RackProduct # Capacity Description M

icro

pla

te

Ve

sse

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pa

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(m

L)

Ve

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Ve

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GX

-28

1 L

H/I

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GX

-27

1 L

H/I

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21

5 L

H/I

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Mu

ltip

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15 L

H/I

nj

GX

-27

4 L

H

GX

-27

1 L

H

22

3 S

am

ple

Ch

an

ge

r

Qu

ad

-Z 2

15

LH

Qu

ad-Z

215

(Disp

osab

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ips)

GX

-27

4 A

SP

EC

GX

-27

1 A

SP

EC

SP

E 2

15

PR

EP

FC

FC

20

4

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03B

Orb

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0 270430 80 - 12 x 32 mm (2 mL) vials 2 80 12 32 X X X

1 12040101 80 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 6 or 9 80 13 65 or 100 X

2 130402 119 - 6 x 50 mm (1 mL) tubes 1 119 6 50 X


7 2707401 1 - 96-well microplate, shallow fl at-bottom X 1 X

8 270438 120 - 6 x 32 mm (0.3 mL) vials 0.3 120 6 32 X X

9 270439 120 - 7 x 40 mm (0.7 mL) vials 0.7 120 7 40 X X

11 170413 105 - 13 x 100 mm (9 mL) tubes 9 105 13 100 X


15 170415 1 - 96-well microplate, shallow X 1 X

16 170416 4 - 96-well microplates, shallow X 4 X

16D 170416D 4 - 96-well microplates, deep X 4 X

17 170418 8 - 24, 48 or 96-well microplates,Whatman Deep Polyfi ltronics Plates X 8 X

20 150425 108 - 10 x 100 mm (4.5 mL) tubes 4.5 108 10 100 X X X X X X X X X X

21 150422 60 - 13 x 100 mm (9 mL) tubes 9 60 13 100 X X X X X X X X X X

22 150424 44 - 18 x 150 mm (25 mL) tubes 25 44 18 150 X X X X X X X X

22U 150498 44 - 10 x 100 mm (4.5 mL) to 18 x 180 mm (32 mL) tubes 4.5 to 32 44 10 to 18 100 to 180 X X X X X X X X

23 150426 44 - 17 x 55 mm (6.8 mL) scintillation vials 6.8 44 17 55 X X X X X X X X X X

23W 270433 44 - 15 x 45 mm (4 mL) WISP vials 4 44 15 45 X X X X X X X X

24 150427 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X X X X X X X X X

28 150420 108 - 10 x 75 mm (3.5 mL) tubes 3.5 108 10 75 X X X X X X X X X X

29 150429 60 - 12 x 75 mm (5 mL) or 13 x 75 mm (7 mL) tubes 5 or 7 60 12 or 13 75 X X X X X X X X X X

29LE 2704342 60 - (1.5 mL) Eppendorf vials 1.5 60 X X X X X X

29SE 2704341 60 - (0.5 mL) Eppendorf vials 0.5 60 X X X X X X

30 2704430 60 - 12 x 32 mm (2 mL) vials 2 60 12 32 X X X X

30P 2704530P 60 - 12 x 32 mm (2 mL ) vials 2 60 12 32 X X

31 2704431 108 - 7 x 40 mm (0.7 mL) vials 0.7 108 7 40 X X X

31P 2704531P 108 - 7 x 40 mm (0.7 mL) vials 0.7 108 7 40 X

32 2704432 60 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 6 or 9 60 13 65 or 100 X X X X

33 2704433 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X X X

33P 2704533P 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X

Gilson Peltier Racks are individually controlled to cool and heat microplates on the bed of a Gilson liquid handler. The racks provide accurate temperature control from 4 to 40˚C.

Product #s:

25146331 (left) (242 Peltier Rack)2514542 (center) (542 Peltier Rack)2515331 (right) (215 Peltier Controller)

The combination of a 542 and a 242 Peltier Rack can accommodate over 1,000 samples on a 215 Liquid Handler.

A single Peltier controller is able to power a 542 and a 242 Peltier Rack or up to fi ve 242 Peltier Racks.

Gilson offers thousands of standard and custom racks to meet the needs of any and all applications. A variety of vessels can be accommodated including tubes, vials, microplates and microchips.

Specifications

Rack Capacity

242: (2) 96-well microplates, shallow542: (10) 96-well microplates, shallow


242: 11.4 x 30.5 x 12.4 cm (4.5 x 12 x 4.9 in)542: 50.1 x 33.6 x 11.4 cm (20.1 x 13.3 x 4.5 in)

Reference


Gilson Rack/Instrument Compatibility Chart continued

Reference

Ra

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icro

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(m

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Ve

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Ve

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Ve

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GX

-28

1 L

H/I

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GX

-27

1 L

H/I

nj

21

5 L

H/I

nj

Mu

ltip

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rob

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15 L

H/I

nj

GX

-27

4 L

H

GX

-27

1 L

H

22

3 S

am

ple

Ch

an

ge

r

Qu

ad

-Z 2

15

LH

Qu

ad-Z

215

(Disp

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ips)

GX

-27

4 A

SP

EC

GX

-27

1 A

SP

EC

SP

E 2

15

PR

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FC

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20

4

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213 2504613 37 - 16 x100 mm (12 mL) tubes + 37 - 16 x 75 mm (10 mL) Vacutainer tubes 12 + 10 37 + 37 16 + 16 100 + 75 X X X X X X X X

214 2504614 48 - 10.25 x 47 mm (2.5 mL) tubes + 48 - 10.25 x 64 mm (3 mL) Vacutainer tubes 2.5 + 3 48 + 48 10.25 +

10.25 47 + 64 X X X X X X X X

216 2504616 60 - 15 x 45 mm (4 mL) WISP vials 4 60 15 45 X X X X X X X X

217 2504617 96 - 10 x 75 mm (4 mL) tubes 4 96 10 75 X X X X X X X X X X X X

218 2504618 2 - 96-well microplates, shallow or deep + 8 - 10 x 75 mm (4 mL) tubes; also holds Beckman modular reservoirs X 4 2 + 8 10 75 X X X X X X X X X X X

219 2504619 27 - 30 x 95 mm (40 mL) vials 40 27 30 95 X X X X X X X X X

220 2504620 70 - 17 x 62 mm (7 mL) vials 7 70 17 62 X X X X X X X

222 2504622 27 - 29 x 115 mm (50 mL) conical bottom tubes 50 27 29 115 X X X X X X X X X

222F 2504622F 24 - 29 x 115 mm (50 mL) conical bottom tubes, w/ funnel drain 50 24 29 115 X X

223 2504623 96 - 16 x 100 mm (12 mL) tubes, w/screw caps 12 96 16 100 X X X X X X X

224 2504624 192 - 8.5 x 41 mm (27 mL) vials 27 192 8.5 41 X X X X X X X

225 2504625 30 - 25 x 150 mm (45 mL) vials 45 30 25 150 X X X X X X X

226 2504626 96 - 15 x 45 mm (4 mL) WISP vials 4 96 15 45 X X X X X X X

228 2504628 4 - (500 mL or 700 mL) bottles 500 or 700 4 X X X X X X X X X X X

234 2504634 2 - Tipack boxes (10 μL or 200 μL tips) 2 X

235 2504635 2 - Tower Pack boxes (10 μL or 200 μL tips) 2 X

237 2504637 2 - 384-well microplates X 2 X X X X X X X X X X X X

239 2504639 40 - 25 x 200 mm (70 mL) tubes 70 40 25 200 X X X X X X

242 25146331 2 - 96-well microplates, shallow X 2 X X X X X X X X X X

300 2504300 96 - (1 mL) cartridges + 1 - 96-well microplate, deep X 1 96 + 1 X X X X X X X X X

301 2504301 96 - (1 mL) cartridges + 1 - 96-well microplate, shallow X 1 96 + 1 X X X X X X X X X

302 2504302 48 - (1 mL) cartridges + 48 - 12 x 32 mm (2 mL) vials 1 + 2 48 + 48 12 32 X X X X X X X X X


304 2504304 48 - (3 mL) cartridges + 48 - 12 x 75 mm (5 mL) tubes 3 + 5 48 + 48 12 75 X X X X X X X X X


306 2504306 20 - (6 mL) cartridges + 20 - 12 x 75 mm (5 mL) tubes 6 + 5 20 + 20 12 75 X X X X X X

307 2504307 20 - (6 mL) cartridges + 20 - 15 x 45 mm (4 mL) vials 6 + 4 20 + 20 15 45 X X X X X X

330 260440031 60 - 12 x 32 mm (2 mL) vials 2 60 12 32 X X X

332 260440079 44 - 18 x 150 mm (25 mL) tubes 25 44 18 150 X X X X X

334 260440081 14 - (40 mL) scintillation vials 40 14 X X X X X

Ra

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icro

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Ve

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GX

-27

1 L

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21

5 L

H/I

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Mu

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15 L

H/I

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4 L

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GX

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1 L

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22

3 S

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15

LH

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215

(Disp

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34 2704434 36 - 15 x 45 mm (4 mL) WISP vials 4 36 15 45 X X

34P 2704534P 36 - 15 x 45 mm (4 mL) WISP vials 4 36 15 45 X

35P 2704535P 71 - 12 x 32 mm (2 mL) vials + 1 - 28 x 60 mm (20 mL) vial 2 + 20 71 + 1 12 + 28 32 + 60 X

36P 2704536P 120 - 7 x 41 mm (0.7 mL) vials + 1 - 28 x 60 mm (20 mL) vial 0.7 + 20 120 + 1 7 + 28 41 + 60 X

37 2704437 56 - 12 x 32 mm (2 mL) vials + 1 - 28 x 60 mm (20 mL) vial 2 + 20 56 + 1 12 + 28 32 + 60 X

81 1904301 2 - 96-well microplates, shallow X 2 X

91 17041950 11 - Kimax or Pyrex (500 mL) bottles 500 11 X

92 17041951 8 - Kimax or Pyrex (1000 mL) bottles 1000 8 X

121 260440086 60 - 13 x 100 mm (9 mL) tubes 9 60 13 100 X X X X

122 260440087 39 - 16 x 100 mm (12 mL) tubes 12 39 16 100 X X X X

143 2954843 20 - (3 mL) DECs 3 20 X X X X X

146 2704146 8 - (6 mL) DECs 6 8 X X X X X

200 2504600 96 - 13 x 100 mm (9 mL) Vacutainer tubes 9 96 13 100 X X X X X X X X X

201 2504601 2 - 96-well microplates + 2 - (1.5 mL) microcentrifuge tubes + 2 - 13 x 100 mm (9 mL) tubes X 1.5 + 9 2 + 2 +2 13 100 X X X X X X X X X X X X

201H 2504601H 2 - 96-well microplates, w/covers + 2 - (1.5 mL) microcentrifuge tubes + 2 - 13 x 100 mm (9 mL) tubes X 1.5 + 9 2 + 2 + 2 13 100 X X X X X X X X X X X X

202 2504602 96 - 10.25 x 47 mm (1.5 mL) microcentrifuge tubes or (2.5 mL) Vacutainer tubes 1.5 or 2.5 96 10.25 47 X X X X X X X

203 2504603 96 - 10.25 x 64 mm (3 mL) tubes 3 96 10.25 64 X X X X X X X X X

204 2504604 27 - 28 x 57 mm (20 mL) scintillation vials 20 27 28 57 X X X X X X X X X

204F 2504604F 24 - 28 x 57 mm (20 mL) scintillation vials 20 24 28 57 X X

205 2504605 2 - 96-well microplates, deep + 2 - (1.5 mL) Eppendorf vials + 2 - 13 x 100 mm (9 mL) tubes X 1.5 + 9 2 + 2 + 2 13 100 X X X X X X X X X X X X

205H 2504605H 2 - 96-well microplates, deep w/covers + 2 - (1.5 mL) Eppendorf vials + 2 - 13 x 100 mm (9 mL) tubes X 1.5 + 9 2 + 2 + 2 13 100 X X X X X X X X X X X X



208 2504608 70 - 18 x 150 mm (25 mL) tubes 25 70 18 150 X X X X X X X X

209 2504609 96 - 12 x 32 mm (2 mL) vials 2 96 12 32 X X X X X X X X X


211 2504611 9 - 48 x 113 mm (125 mL) bottles 125 9 48 113 X X X X X X X X X

211F 2504611F 8 - 48 x 113 mm (125 mL) bottles, w/funnel drain 125 8 48 113 X X

211H 2504611H 9 - 48 x 113 mm (125 mL) bottles, w/cover 125 9 48 113 X X X X X X X X X

212 2504612 48 - 13 x 100 mm (9 mL) tubes + 48 - 13 x 75 mm (7 mL) Vacutainer tubes 9 + 7 48 + 48 13 + 13 100 + 75 X X X X X X X X X


Tip Design Description Features

Flat The tip is cut perpendicular to the length of the probe. · Aspirates the last drop of liquid· Does not pierce septum· No injection

Constricted The tip of the probe has a smaller outer diameter than the rest of the shaft. Constricted-tip probes accommodate unique tip designs optimizing every application.

· Compatible with injection ports

Beveled The tip is cut at an angle, leaving a bevel. · Sharp tip enables septum-piercing· Compatible with injection ports

Spring Loaded Low volume, touch-off dispensing. · Ideal for MALDI spotting applications· Spring loaded probe removes any potential of damaging spotting surface

Vented Two styles: 1. A shallow groove cut in the probe shaft, above the tip

(shown).2. Two probes are assembled concentrically, creating two

separate channels, the inner channel for liquid and the outer channel for gas.

Vented probes may have diff erent tip designs.

· Sharp-tip enables septum-piercing· Vent allows pressures inside and outside a sealed vessel to

equilibrate as liquid is dispensed or aspirated

Side-entry The liquid fl ow path opens on the side of the probe, not at the tip.

· Recommended for thick septa· Prevents coring of septa· Compatible with injection ports · Not recommended with limited volume

Defl ected Based on the beveled tip design, the probe tip is curved and very sharp.

· Sharp-tip enables septum-piercing· Tip pushes septa out of the way as it pierces to prevent coring

Conical Designed for PEEK injection ports. · Capable of piercing thin septa· Aspirates the last drop of liquid

Reference

Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing solutions for all liquid handling requirements.

Probes

Ra

ck C

od

e


icro

pla

te

Ve

sse

l Ca

pa

city

(m

L)

Ve

sse

l Qu

an

tity

Ve

sse

l Dia

me

ter

Ve

sse

l He

igh

t

GX

-28

1 L

H/I

nj

GX

-27

1 L

H/I

nj

21

5 L

H/I

nj

Mu

ltip

le P

rob

e 2

15 L

H/I

nj

GX

-27

4 L

H

GX

-27

1 L

H

22

3 S

am

ple

Ch

an

ge

r

Qu

ad

-Z 2

15

LH

Qu

ad-Z

215

(Disp

osab

le T

ips)

GX

-27

4 A

SP

EC

GX

-27

1 A

SP

EC

SP

E 2

15

PR

EP

FC

FC

20

4

FC 2

03B

Orb

ita

l Sh

ak

er

335 260440083 48 - 15 x 45 mm (4 mL) WISP vials 4 48 15 45 X X X X X X

336 260440094 2 - 96-well microplates, shallow or deep X 2 X X X X X


338 260440106 64 - 12 x 32 mm (2 mL) vials 2 64 12 32 X X X X X

341 260440039 108 - 10 x 75 mm (3.5 mL) tubes 3.5 108 10 75 X X X X X



371 2604701 36 - (1 mL) DECs 1 36 X X X X

373 2604702 20 - (3 mL) DECs 3 20 X X X X

376 2604703 15 - (6 mL) DECs 6 15 X X

502 2504652 100 - 12 x 32 mm (2 mL) vials (1 - HP 1100LC tray) 2 100 12 32 X X


505H 2504651H 10 - 96-well microplates, shallow or deep w/covers X 10 X X X X X

517 2504653 17 - 96-well microplates, shallow or deep X 17 X X

542 2514542 10 - 96-well microplates, shallow X 10 X X X X

821 2504821 48 - (1 mL) tabbed SPE cartridges 1 48 X

822 2504822 96 - (1 mL) tabless SPE cartridges 1 96 X

823 2504823 48 - (3 mL) tabless SPE cartridges 3 48 X

824 2504824 1 - 96-well wide SPE plate, compatible with porvair-style plates such as Varian & Waters 1 X

825 2504825 1 - 96-well narrow SPE plate, compatible with plates such as 3M & Ansys Spec. 1 X

852 2514852 2 - 96-well Becton Dickinson Falcon™ "U-bottom" microplates, shallow X 2 X X X X X X X X

853 2514853 96 - 12 x 32 mm (2 mL) vials, fl at-bottom 2 96 12 32 X X X X X X

854 2514854 2 - 96-well Beckman or Ritter microplates, deep X 2 X X X X X X X X

VR 2514701 SPE extraction plates: Waters OASIS®, 3M Empore™ and Varian VersaPlate™ X

M 1907152 223 Maldi Rack, 1 - Bruker MALDI Plate X

Gilson Rack/Instrument Compatibility Chart continued

Gilson Probe Product Number Identifi cation

Gilson probes are laser engraved with their product number on the collar of each probe providing a quick and effortless identification reference for re-ordering and support.


Product # Septum-piercing Tip Shape MaterialDimensions

(mm, L x OD x ID)Injection Compatibility Instrument Compatibility

125 mm Z-arm continued

2507242 X Defl ected Stainless steel, Tefl on® interior 274 x 1.8 x 0.8 GX Z-Injection Module

GX-271, GX-274, GX-281,215

2507243 X Defl ected Stainless steel, Tefl on® interior 274 x 1.8 x 0.8 GX Z-Injection Module

GX-271, GX-274, GX-281,215

2507244 X Defl ected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection ModuleGX-271, GX-274, GX-281,215

2507245 X Defl ected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection ModuleGX-271, GX-274, GX-281,215

2507248 Conical, micro- volume Stainless steel 220 x 2.1 x 0.4 841 215

2507252 Micro septum Constricted, beveled Stainless steel 220 x 1.5 x 0.4

Tip: 10 x 0.7 x 0.4

819 (requires InjectionPort #25051213)849 #250512861

215, Multiple Probe 215

2507256 X Beveled Stainless steel 221 x 1.5 x 0.4

819 (requires InjectionPort #2954640)GX Direct Injection Module (requires Injection Port #26035411)

GX-271, GX-274, GX-281,GX-271 ASPEC,GX-274 ASPEC,215

2507414 Constricted, fl at Stainless steel 220 x 1.3 x 0.8

819 (requires InjectionPort #25051015)849 (requires InjectionPort Bar #250512862)GX Direct Injection Module (requires Injection Port #26035410)

GX-271, GX-274, GX-281,215, Multiple Probe 215

27067361 Beveled Stainless steel 220.5 x 1.5 x 1.1

819 (requires InjectionPort #2954640)849 #250512863GX Direct Injection Module (requires Injection Port #26035411)

GX-271, GX-274, GX-281,215, Multiple Probe 215



GX-271, GX-274, GX-281,215, Multiple Probe 215232 XL/233 XL

27067382 X Beveled Stainless steel 221 x 2 x 0.8Tip: 2.1 x 1.5 x 0.8


GX-271, GX-274, GX-281,215, Multiple Probe 215 (183 mm arm)

27067383 X Beveled Stainless steel 221 x 1.5 x 0.4


GX-271, GX-274, GX-281,215, Multiple Probe 215(183 mm arm) , GX-271 GPC Systems

56 mm Z-arm

496215 Flat Stainless steel 51 x 0.7 x 0.4

27067251 X Beveled Stainless steel 103 x 0.7 x 0.4 Requires Injection Port #2954645 231 XL

27067113 Constricted, fl at Stainless steel 93.5 x 1.5 x 1.1 GX-271, GX-274,223, 231 XL

27067131 X Beveled Stainless steel 95 x 0.7 x 0.42 Requires Injection Port #270728 & Probe Sleeve #27067132

223, 231 XL/232 XL/233 XL

27067133 X Beveled Titanium 95 x 0.7 x 0.42 Requires Injection Port #270728 Requires Sleeve #27067132

223, 231 XL/232 XL/233 XL

123 mm Z-arm

27067211 Beveled Stainless steel 160.5 x 1.5 x 1.1

27067212 X Side-entry Stainless steel 160.5 x 1.5 x 1.1 Requires Injection Port #2954645223, 221 XL,231 XL/232 XL/233 XL,ASTED XL

27067213 Tapered, fl at Stainless steel 160.5 x 1.5 x 1.1 223, 221 XL

Reference



175 mm Z-arm

2507214 Constricted, fl at Stainless steel 269 x 1.8 x 1.4 GX Z-Injection ModuleGX-271, GX-274 GX-281,215, 215 SW,Multiple Probe 215


819 (requires InjectionPort #25051015)849 (requires Injection Port #25051213)GX Direct Injection Module (requires Injection Port #26035410)

GX-271, GX-274, GX-281,215, 215 SW,Multiple Probe 215,Quad-Z 215

2507216 Beveled,level-sensing Stainless steel 269 x 1.5 x 1.1

Tip: 1.6 x 1.2 x 0.8

819 (requires InjectionPort #2954674)849 #25051215Seal #2954674GX Direct Injection Module (requires Injection Port #26035410)

GX-271, GX-274, GX-281,215, 215 SW,Multiple Probe 215,Quad-Z 215

2507247 X Beveled Stainless steel 323 x 1.8 x 0.8 215 SPE

2507249 Conical,micro-volume Stainless steel 269 x 2.4 x 0.4 215

2507253 Micro septum Beveled, level-sensing Stainless steel 269 x 1.5 x 0.4

Tip: Con Bev Micro 0.6819 (requires InjectionPort #270728)

GX-271, GX-274, GX-281,Quad-Z 215

2507254 Flat, level-sensing Stainless steel 269 x 1.8 x 1.4 GX Z-Injection ModuleGX-271, GX-274, GX-281,215, 215 SW,Quad-Z 215

2507255 Beveled, level-sensing Stainless steel 269 x 1.5 x 0.4


GX-271, GX-274, GX-281, GX-271 ASPEC,GX-274 ASPEC,215, 215 SW,Quad-Z 215

25073645 Beveled Stainless steel 269 x 1.3 x 0.8

819 (requires InjectionPort #250510153)849 (requires InjectionPort Bar #250512862)

GX-271, GX-274, 215,215 SW,Multiple Probe 215,Quad-Z 215

2507555 FlatStainless steel, Tefl on® coated (plastic tip)

272.5 x 2.7 x 0.8Tip: 10 GX Z-Injection Module

GX-271, GX-274, GX-281,215

251646 Inert Gas Probe X Beveled Stainless steel 317 x 1.8 x 0.8 GX Z-Injection Module

GX-271, GX-274, GX-281,215

25075551 Flat Stainless steel 256.6 x 2.7 x 0.8 GX Z-Injection ModuleGX-271, GX-274, GX-281,215

26017050 X Beveled Stainless steel 269 x 2 x 0.8Tip: 1.5 OD x 0.8


GX-271, GX-274, GX-281,215, 215 SW

125 mm Z-arm

2507234 X Side-entry Stainless steel 274 x 1.8 x 0.8819 (requires InjectionPort #25051015)GX Z-Injection Module

GX-271, GX-274, GX-281,215


GX-271, GX-274, GX-281,215

2507236 X Side-entry Stainless steel 272.5 x 1.5 x 0.8819 (requires InjectionPort #25051015)GX Z-Injection Module

GX-271, GX-274, GX-281,215


GX-271, GX-274, GX-281,215

Gilson Probe Selection Guide


1H

1.008

2He

4.003

87Fr

(223)

103Lr

(260)

102No

(259)

101Md

(258)

100Fm

(257)

99Es

(252)

98Cf

(251)

97Bk

(247)

96Cm

(247)

95Am

(243)

71Lu

175.0

70Yb

173.0

69Tm

168.9

68Er

167.3

67Ho

164.9

66Dy

162.5

65Tb

158.9

64Gd

157.3

63Eu

152.0

94Pu

(244)

93Np

237.0

62Sm

150.4

61Pm

(145)

92U

238.0

91Pa

231.0

60Nd

144.2

59Pr

140.9

90Th

232.0

58Ce

140.1

**Actinides

*Lanthanides

Undiscovered Synthetic From Decay Primordial

*

**

105Ha

(262)

104Rf

(261)

89Ac

227.0

88Ra

226.0

109Une

(276) (281) (280) (285) (284) (289) (288) (291) (294)Not Yet Observed(277)

107Uns

108Hs

110Ds

111Rg

112Uub

113Uut

114Uuq

115Uup

116Uuh

117Uus

118Uuo

(262)

106Unh

(263)

55Cs

132.9

86Rn

(222)

85At

(210)

84Po

(209)

83Bi

209.0

82Pb

207.2

81Tl

204.4

80Hg

200.6

79Au

197.0

78Pt

195.1

73Ta

180.9

72Hf

178.5

57La

138.9

56Ba

137.3

77Ir

192.2

76Os

190.2

75Re

186.2

74W

183.9

37Rb

85.47

54Xe

131.3

53I

126.9

52Te

127.6

51Sb

121.8

50Sn

118.7

49In

114.8

48Cd

112.4

47Ag

107.9

46Pd

106.4

41Nb

92.91

40Zr

91.22

39Y

88.91

38Sr

87.62

45Rh

102.9

44Ru

101.1

43Tc

(98)

42Mo

95.94

19K

39.10

36Kr

83.80

35Br

79.90

34Se

78.96

33As

74.92

32Ge

72.59

31Ga

69.72

30Zn

65.38

29Cu

63.55

28Ni

58.70

23V

50.94

22Ti

47.90

21Sc

44.96

20Ca

40.08

27Co

58.93

26Fe

55.85

25Mn

54.94

24Cr

52.00

11Na

23.00

18Ar

39.95

17Cl

35.45

16S

32.06

15P

30.97

14Si

28.09

13Al

26.98

12Mg

24.31

3Li

6.941

10Ne

20.18

9F

19.00

8O

16.00

7N

14.01

6C

12.01

5B

10.81

4Be

9.012

1A 8A

2B1B5B4B3B 8B7B6B

7A6A5A4A3A2A

Capillary Flow Cell Micro-bore Flow Cell Analytical Flow Cell Semi-preparative Flow Cell Preparative Flow Cell

Product # 104446 104442 104445 104441 104443

Internal Volume 35 nL 1.5 μL 12 μL 0.7 μL 0.16 μL

Pathlength 8 mm 2 mm 5 mm 0.2 mm 0.05 mm

Column Size 180 – 300 μm 1 – 2 mm 4 mm

10 mm

20 mm > 40 mm

Flow Rate 1 – 10 μL/min 25 – 200 μL/min 1 – 15 mL/min 15 – 50 mL/min 30 – 200 mL/min

Purify μg - 5 mg Purify 5 – 300 mg Purify 300 mg - 15 g

Selecting the correct flow cell will optimize sensitivity and peak shape. The following chart pairs flow cells with theappropriate column, flow rate and sample concentration to help choose the correct flow cell for the application.

Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors

Periodic Table of the Elements

Reference



123 mm Z-arm continued

27067217 Flat Tefl on® coated (plastic tip)

157.5 x 0.9 (18 g)Tip: 10 223


157.5 x 0.9 (22 g)Tip: 10 223

27067231 Tapered, beveled Stainless steel 162 x 0.7 x 0.42 Requires Injection Port #270728 & Probe Sleeve #27067232

223,231 XL/232 XL/233 XL,ASTED XL

27067235 X Beveled Stainless steel 162 x 0.7 x 0.42 Requires Injection Port #270728 & Probe Sleeve #27067236

223,231 XL/232 XL/233 XL,ASTED XL

27067237 X Beveled Titanium 162 x 0.7 x 0.42 Requires Injection Port #270728 & Probe Sleeve #27067239

231 XL,ASTED XL

27067238 X Beveled Stainless steel 162 x 0.7 x 0.4 Requires Injection Port #270728223,231 XL/232 XL/233 XL,ASTED XL

27067272 Tapered, beveled Stainless steel 160.5 x 1.5 x 1.1 Requires Injection Port #2954645 223,231 XL/232 XL/233 XL,ASTED XL

27167215 Double probe kit X Beveled Stainless steel probe 1: 181.5 x 1.5 x 1.1

probe 2: 169.5 x 2.5 x 1.1


157.5 x 0.9Tip: 10

183 mm Z-arm

27067361 Beveled, level-sensing Stainless steel 220.5 x 1.5 x 1.1

819 (requires InjectionPort #2954640)232 XL/233 XL (requires Injection Port #2954645)

215, 223,232 XL/233 XL

27067362 X Side-entry Stainless steel 220.5 x 1.5 x 1.1 Requires Injection Port #2954645 223, 232 XL/233 X

27067373 Constricted, fl at,level-sensing Stainless steel 221 x 1.5 x 1.1

Tip: 2 x 1.1 x 0.4

819 (requires InjectionPort #2954640)849 #250512863889 #250512753

215, Multiple Probe 215,223, 232 XL/233 XL

27367423 Flat Tefl on® coated 220.5 x 0.9 232 XL/233 XL

210 mm Z-arm

26037001 X Beveled Stainless steel 304 x 2 x 0.8Tip: 2.1 x 1.5 x 0.8

GX Direct Injection Module (requires Injection Port #26035411)

GX-281

260370011 X Beveled Stainless steel (coated)

301.3 x 2 x 0.8 Tip: 2.1 x 1.5 x 0.8


GX-281

26037002 X Beveled Stainless steel 304 x 1.5 x 0.4GX Direct Injection Module (requires Injection Port #26035411)

GX-281

260370021 X Beveled Stainless steel (coated) 301.3 x 1.5 x 0.4


GX-281

26037004 X Beveled Stainless steel 304 x 1.5 x 0.4GX Direct Injection Module (requires Injection Port #26035411)

GX-281

26037005 Beveled Stainless steel 304 x 1.5 x 1.1GX Direct Injection Module (requires Injection Port #26035411)

GX-281

26037006 Flat Stainless steel 304 x 2.4 x 1.8 GX-281

26037007 Flat Stainless steel 304 x 1.8 x 1.4 GX-281

Gilson Probe Selection Guide continued


Maximum UV Absorbance for Commonly Used Solvents

Operating at a wavelength near or below a mobile phase solvent’s maximum UV absorbancy increases baseline noise due to the UV response of the solvent. Refer to the tables below to optimize chromatographic conditions.

Table 2

Mobile Phase

Conditioners & Buff ers

Maximum UV

Absorbance (nm)

Acetic Acid, 1% 230

Ammonium Acetate, 10 mM

205

Ammonium Bicarbonate, 10 mM

190

Diammonium Phosphate, 50 mM

205

EDTA, Disodium, 1 mM 190

HEPES, 10 mM, pH 7.6 225

Hydrochloric acid, 0.1% 190

MES, 10 mM, pH 6.0 215

Potassium PhosphateMonobasic, 10 mM

Dibasic, 10 mM190190

Sodium Acetate, 10 mM 205

Sodium Chloride, 1M 207

Sodium Citrate, 10 mM 225

Sodium Dodecyl Sulfate 190

Sodium Formate 200

Triethylamine, 1% 235

Trifl uoracetic Acid, 0.1% 190

TRIS HCl, 20 mMpH 7.0pH 8.0

202212

Reference

pH of Common Acids

Acids (pH < 7) Molarity pH

Acetic 1 N 2.4

Acetic 0.1 N 2.9

Acetic 0.01 N 3.4

Alum 0.1 N 3.2

Arsenious Saturated 5

Benzoic 0.1 N 3

Boric 0.1 N 5.3

Carbonic Saturated 3.8

Citric 0.1 N 2.1

Formic 0.1 N 2.3

Hydrochloric 1 N 0.1

Hydrochloric 0.1 N 1

Hydrochloric 0.01 N 2

Hydrocyanic 0.1 N 5.1

Hydrogen Sulfi de 0.1 N 4.1

Lactic 0.1 N 2.4

Malic 0.1 N 2.2

Nitric 0.1 N 1

Orthophosphoric 0.1 N 1.5

Oxalic 0.1 N 1.3

Succinic 0.1 N 2.7

Salicylic Saturated 2.4

Sulfuric 1 N 0.3

Sulfuric 0.1 N 1.2

Sulfuric 0.01 N 2.1

Sulfurous 0.1 N 1.5

Tartaric 0.1 N 2

Trichloracetic 0.1 N 1.2

pH of Common Bases

Bases (pH > 7) Molarity pH

Ammonia 1 N 11.6

Ammonia 0.1 N 11.1

Ammonia 0.01 N 10.6

Barbital Sodium 0.1 N 9.4

Borax 0.01 N 9.2

Calcium Carbonate Saturated 9.4

Calcium Hydroxide Saturated 12.4

Ferrous Hydroxide Saturated 9.5

Lime Saturated 12.4

Magnesia Saturated 10.5

Potassium Acetate 0.1 N 9.7

Potassium Bicarbonate 0.1 N 8.2

Potassium Carbonate 0.1 11.5

Potassium Cyanide 0.1 N 11

Potassium Hydroxide 1 N 14

Potassium Hydroxide 0.1 N 13

Potassium Hydroxide 0.01 N 12

Sodium Acetate 0.1 N 8.9

Sodium Benzoate 0.1 N 8

Sodium Bicarbonate 0.1 N 8.4

Sodium Carbonate 0.1 N 11.6

Sodium Hydroxide 1 N 14

Sodium Hydroxide 0.1 N 13

Sodium Hydroxide 0.01 N 12

Sodium Metasilicate 0.1 N 12.6

Sodium Sesquicarbonate 0.1 N 10.1

Trisodium Phosphate 0.1 N 12

Internal Diameter (mm) Typical Injection Volume (μL) Load Capacity (mg) Typical Flow Rates (mL/min)

4.6 15 1 0.5 – 2

10 100 4.7 4 – 15

21.2 400 19.5 10 – 50

30 1,000 42.5 40 – 100

50 2,000 118 100 – 300

100 10,000 473 400 – 1000

pH of Acids and Bases

Column Sizes and Capacities

Table 1

Solvent Maximum UV Absorbance (nm)

1-Nitropropane 380

2-Butoxyethanol 220

Acetone 330

Acetonitrile 190

Amyl Alcohol 210

Amyl Chloride 225

Benzene 280

Butanol-1 215

Butanol-2 260

n-Butyl Chloride 220

Carbon Disulfi de 380

Carbon Tetrachloride 265

Chloroform 245

Clorobenzene 287

Cyclohexane 200

Cyclopentane 200

Diethyl Amine 275

Dimethyl Sulfoxide 268

Dioxane 215

Ethanol 210

Ethyl Acetate 256

Ethyl Ether 220

Ethyl Sulfi de 290

Ethylene Dichloride 230

Ethylene Glycol 210

Heptane 200

Hexane 195

Isooctane 215

Isopropanol 205

Isopropyl Chloride 225

Isopropyl Ether 220

Methanol 205

Methyl Acetate 260

Methyl Ethyl Ketone 330

Methyl Isobutyl Ketone 334

Methylene Chloride 233

n-Pentane 190

n-Propanol 210

n-Propyl Chloride 225

Nitromethane 380

Petroleum Ether 210

Pyridine 330

Tetrahydrofuran 230

Toluene 285

Xylene 290


Separation Scale-up Equations

Scale-up Factor (diameter preparative)2 scale-up factor = __________________________ (diameter analytical)2

Flow Rate scale-up factorflow rate preparative = __________________________

analytical flow rate

Gradient Duration (GD) length preparative (diameter preparative)2 flow rate analyticalGD preparative = GD analytical x __________ x _____________ x ____________ length analytical (diameter analytical)2 flow rate preparative

Reference

Table 2: Rheodyne 7010 – 156 Factory Installed Large-bore Rotor Seal

Flow Rate

(mL/min)

Valve Position and Pressure

Standard 7010 Bore Size 7010 with Large-bore Rotor

Load Position

Pressure (psi)

Inject Position

Pressure (psi)

Gilson Installed

Load Position

Pressure (psi)

Gilson Installed

Inject Position

Pressure (psi)

25 0 14.5 0 0 – 14.5

50 0 43.5 0 43.5

75 0 87 0 116

100 14.5 145 14.5 203.1

150 43.5 290 72.5 435.1

200 87 435.1 145 696.2

Optimizing Preparative HPLC

There are numerous parameters and techniques to optimize preparative HPLC. These reference tables provide important physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent recovery of collected fractions.

Table 1: 33X Series pump tubing (PEEK)*

Flow Rate (mL/min)Tubing ID (in)

0.02 Pressure (psi) 0.03 Pressure (psi) 0.04 Pressure (psi)

25 0 0 0

50 43.5 0 0

75 101.5 0 0

100 203.1 14.5 0

125 333.6 43.5 0

150 464.1 87 14.5

175 623.7 101.5 14.5

200 797.7 145 29

At high flow rates tubing can cause high back pressure. Ensuring that the appropriate tubing ID/flow rate combination is used will optimize the pumping system.

*30" lengths of tubing used at corresponding fl ow rates for tubing pressure.

Tubing was connected directly to the pump. All pre-fi lters were removed.

Product #: 25051043 (7010 valve with large-bore rotor seal)

· 0.018" make-before-break stator orifi ce · 1 mL stainless steel 0.030" ID loop · All other plumbing used is stainless steel

0.040" ID tubing

Solvent Miscibility TableSolvent Polarity

IndexRefraction

Index @20°C

UV(nm) Cut-off @1AU

Boiling Point (°C)

Viscosity (cPoise)

Solubility in water (%w/w)

Acetic Acid 6.2 1.372 230 118 1.26 100Acetone 5.1 1.359 330 56 .032 100Acetonitrile 5.8 1.344 190 82 0.37 100Benzene 2.7 1.501 280 80 0.65 0.18n-Butanol 4.0 1.394 254 125 0.73 0.43Butyl Acetate 3.9 1.399 215 118 2.98 7.81Carbon Tetrachloride 1.6 1.466 263 77 0.97 0.08Chloroform 4.1 1.446 245 61 0.57 0.815Cyclohexane 0.2 1.426 200 81 1.00 0.011,2-Dichloroethane1 3.5 1.444 225 84 0.79 0.81Dichloromethane2 3.1 1.424 235 41 0.44 1.6Dimethylformamide 6.4 1.431 268 155 0.92 100Dimethyl Sulfoxide3 7.2 1.478 268 189 2.00 100Dioxane 4.8 1.422 215 101 1.54 100Ethanol 5.2 1.360 210 78 1.20 100Ethyl Acetate 4.4 1.372 260 77 0.45 8.7Di-Ethyl Ether 2.8 1.353 220 35 0.32 6.89Heptane 0.0 1.387 200 98 0.39 0.0003Hexane 0.0 1.375 200 69 0.33 0.001Methanol 5.1 1.329 205 65 0.60 100Methyl-t-Butyl Ether4 2.5 1.369 210 55 0.27 4.8Methyl Ethyl Ketone5 4.7 1.379 329 80 0.45 24Pentane 0.0 1.358 200 36 .23 .004n-Propanol 4.0 1.384 210 97 2.27 100Iso-Propanol6 3.9 1.377 210 82 2.30 100Di-Iso-Propyl Ether 2.2 1.368 220 68 0.37Tetrahydrofuran 4.0 1.407 215 65 0.55 100Toluene 2.4 1.496 285 111 0.59 0.051Tichloroethylene 1.0 1.477 273 87 0.57 0.11Water 9.0 1.333 200 100 1.00 100Xylene 2.5 1.500 290 139 0.61 0.018

Xyle

neW

ater

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lene

Tolu

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Acet

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ileAc

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Immiscible

Miscible

Immiscible means that in some proportions two phases will be produced.

Synonym Table1 Ethylene Chloride2 Methylene Chloride3 Methyl Sulfoxide4 tert-Butyl Methyl Ether5 2-Butanone6 2-Propanol

Solvent Miscibility and Polarity

Solvent Polarity Chart

Relative Polarity

Compound Formula

Group Representative Solvent Compounds

Non-polar R - H Alkanes Petroleum Ethers, Ligroin, Hexanes

Incr

easi

ng P

olar

ity

Ar - H Aromatics Toluene, Benzene

R - O - R Ethers Diethyl Ether

R - X Alkyl Halides Tetrachloromethane, Chloroform

R - COOR Esters Ethyl Acetate

R - CO - R Aldehydes and Ketones Acetone, Methyl Ethyl, Ketone

R - NH 2 Amines Pyridine, Triethylamine

R - OH Alcohols Methanol, Ethanol, Isopropanol, Butanol

R - COHN 2 Amides Dimethylformamide

R - COOH Carboxylic Acids Ethanoic Acid

Polar H - OH Water Water


Common Conversion Tables

Reference

Temperature Conversion Formulas

˚C = ˚F – 32 ÷ 1.8

˚C = degrees Celsius˚F = degrees Fahrenheit

˚K = degrees Kelvin˚R = degrees Rankine

˚C = ˚K – 273.15

˚F = 1.8 x ˚C + 32

˚F = ˚R – 459.67

˚K = ˚C + 273.15

˚R = ˚F + 459.67

Metric Prefixes

Prefi x Symbol Scientifi c Notation

yotta Y 1024

zetta Z 1021

exa E 1018

peta P 1015

tera T 1012

giga G 109

mega M 106

kilo k 103

hecto h 102

deka da 10

deci d 10-1

centi c 10-2

milli m 10-3

micro μ 10-6

nano n 10-9

pico p 10-12

femto f 10-15

atto a 10-18

zepto z 10-21

yocto y 10-24

Tubing Internal Diameters & Volumes

Inch Millimeter Micron μL/in μL/mm

0.001 0.025 25 0.013 0.0005

0.002 0.051 51 0.051 0.002

0.003 0.076 76 0.116 0.0046

0.004 0.102 102 0.206 0.0081

0.005 0.127 127 0.322 0.0127

0.006 0.152 152 0.463 0.0182

0.007 0.178 178 0.631 0.0248

0.008 0.203 203 0.824 0.0324

0.009 0.229 229 1.042 0.041

0.01 0.254 254 1.287 0.0507

0.011 0.279 279 1.557 0.0613

0.012 0.305 305 1.853 0.073

0.013 0.33 330 2.175 0.0856

0.014 0.356 356 2.523 0.0993

0.015 0.381 381 2.896 0.114

0.016 0.406 406 3.295 0.1297

0.017 0.432 432 3.72 0.1464

0.018 0.457 457 4.17 0.1642

0.019 0.483 483 4.646 0.1829

0.02 0.508 508 5.148 0.2027

0.028 0.711 711 10.09 0.3973

0.03 0.762 762 11.583 0.456

0.032 0.813 813 13.179 0.5189

0.04 1.016 1016 20.593 0.8107

0.042 1.067 1067 22.703 0.8938

0.055 1.397 1397 38.933 1.5328

0.062 1.575 1575 49.474 1.9478

Pressure Conversion

kPa psi bar MPa

690 100 6.9 0.7

3450 500 34.5 3.4

6890 1000 68.9 6.9

10340 1500 103.4 10.3

13790 2000 137.9 13.8

17240 2500 172.4 17.2

20680 3000 206.8 20.7

24130 3500 241.3 24.1

27580 4000 275.8 27.6

31030 4500 310.3 31

34470 5000 344.7 34.5

37920 5500 379.2 37.9

41370 6000 413.7 41.4

44820 6500 448.2 44.8

48260 7000 482.6 48.3

51710 7500 517.1 51.7

55160 8000 551.6 55.2

58610 8500 586.1 58.6

62050 9000 620.5 62.1

65500 9500 655 65.5

68950 10000 689.5 68.9

Temperature Conversion

Celsius (°C) Fahrenheit (°F)

-100 -148

-50 -58

-40 -40

-30 -22

-20 -4

-10 14

0 32

5 41

10 50

15 59

20 68

25 77

30 86

35 95

40 104

45 113

50 122

55 131

60 140

65 149

70 158

75 167

80 176

85 185

90 194

95 203

100 212

105 221

110 230

115 239

120 248

125 257

130 266

135 275

140 284

145 293

150 302

155 311

160 320

165 329

170 338

175 347

180 356

185 365

190 374

195 383

200 392

Fractional inch-to-decimal-to-millimeter Conversion

Fraction (") Decimal Millimeter

1/32 .0313 .7938

1/16 .0625 1.5875

3/32 .0938 2.3813

1/8 .125 3.175

5/32 .1563 3.9688

3/16 .1875 4.7625

7/32 .2188 5.5563

1/4 .25 6.35

9/32 .2813 7.1438

5/16 .3125 7.9375

11/32 .3438 8.7313

3/8 .375 9.525

13/32 .4063 10.3188

7/16 .4375 11.1125

15/32 .4688 11.9063

1/2 .5 12.7

17/32 .5313 13.4938

9/16 .5625 14.2875

19/32 .5938 15.0813

5/8 .625 15.875

21/32 .6563 16.6688

11/16 .6875 17.4625

23/32 .7188 18.2563

3/4 .75 19.05

25/32 .7813 19.8438

13/16 .8125 20.6375

27/32 .8438 21.4313

7/8 .875 22.225

29/32 .9063 23.0188

15/16 .9375 23.8125

31/32 .9688 24.6063

1 1 25.4


Liquid Chromatography continued

Automated Post-collection Concentration for Purified Preparative Fractions via SPEThe method (as opposed to analytical applications involving SPE) works by collecting and then diluting the fraction(s), passing the fraction(s) through an SPE and ultimately eluting the concentrated fraction in a small amount of 100% organic solvent.

An Automated Neoteric Approach to Trapping Metabolites for NMR AnalysisSPE cartridges are used to trap the metabolites from natural occurring material during a repetitive analytical run with injection of the same sample; in order to concentrate the individual metabolites to a level required for NMR analysis.

A Chiral Piece of the Pie-Optimizing Chiral Separations through Selective Sample Slicing and Column SwitchingSamples are injected onto a primary column and individual peaks are cleaved and transferred onto a secondary chiral column for separation. We demonstrate complete purification of analytes with high resolution of the separated enantiomers

Increasing Throughput of GPC Clean-Up via a Parallel Sample Extraction ProcessThe purpose of this study was to evaluate the effectiveness of developing an automated GPC clean-up system that is capable of running up to four sample extracts in parallel, along with fraction collection, thus improving sample throughput.

Optimization of Steady State Recycle SSR: Parameters Utilizing Polarimetry in Chiral SeparationsProcessing gram to kilogram quantities of target analytes has led to the exploration of several high throughput separation techniques. Among those investigated is Steady State Recycle SSR. Similar to Simulated Moving Bed SMB fractions are collected from the leading and trailing edges of a chromatographic contour while sample material is injected into the interior. The difference SMB is a continuous process while SSR is a repetitive process.

Flash: Eliminating the Concentration/Dry-down Required for a Purified Compound by Preparative ChromatographyEmploying an activated media packed in an SPE/Flash cartridge (60 mL/10 gm), the automated system can intelligently identify the purified fractions and compound mass 0.1 – 2 g, without time-consuming dry-down.

Fast PCLC: A widely Applicable Technique for Laboratory Scale Preparative SeparationsIt is often expected that prep HPLC can be satisfactorily performed via low efficiency, large diameter columns, operated under overload conditions. Evaluation of this preparative HPLC, low-tech route is not the best option. Short, fine particle columns permit baseline resolution of closely eluting compounds. Incorporating gradient elution and multicycle automation, the 1-inch bore can isolate 1 mg of each of several substances, but can also be employed to obtain up to 200 grams of a single compound. This technique, called FAST PCLC (preparative column liquid chromatography), reduces analysis time, so throughput is greater. For example, a 3 um column can handle a throughput of 1 gram in about one-sixth of the time required on a 30 um column.

Multiple Column Injection and Single Concentration Column Fraction collection to increase sample volume and Speed up Dry DownPresented is an application to separate flavonoids via a multiple column injection. The sample injection is optimized for peak resolution on a Prep C18 Column. Identical columns are configured in parallel for injections sequenced by the sample peak elution of the test column. Sample is concentrated on separate C18 prep columns and eluted in high organic for effective dry down of the target sample. This technique provides high throughput of sample for each cycle decreasing separation time to purify gram quantities of target compounds.

FTLC: Automated Fraction trapping of Purified Compounds from Preparative Chromatography via a Specialized Polymer Resin packed within a HPLC columnThis trapping method allows for fractions collected to undergo transformation into 100% organic solvent without operator intervention, drastically reducing the dry down time and eliminating hydrolysis of the compound. The HPLC trapping column has been shown to be very robust and rugged stable for 1000s of fraction trapping samples.

Reference

cLC™ System: An Automated Solution for Normal and Reversed-phase Preparative HPLC with Analytical Purification DeterminationThe cLC system offers researchers the capabilities of both RP and NP chromatography on a single system, including data representing the throughput and analysis capabilities of the system.

Optimizing Purified Sample Recovery on Reversed-phase HPLC SystemsThis application presents the factors that can directly affect purity and recovery when processing compounds on a preparative level. The following system parameters were evaluated: collection valve switch time, detector time constant delay, DMSO sandwich solvent, delay volume from detector to collection valve and software response.

Optimizing Chiral Separations via an Automated Parallel Scouting System for On-line Preparative Chiral PurificationThe system is capable of screening chiral compounds on four separate columns in parallel, increasing the stereoselective screening by four fold.

Determination of Reproducibility within an Analytical System via Repeat InjectionsThe data presented in this application shows that the Gilson HPLC system provides consistent and reproducible chromatography. A mixture of three compounds was injected repeatedly under analytical reversed-phase conditions.

A Multi-purpose Preparative System for the Detection and Collection of Active and Non-active UV/VIS CompoundsThe process relies on an analytical scout analysis to determine the best gradient conditions to optimize the separation of the compounds under preparative conditions and minimize the number of fractions collected per run by incorporating ELS detection in addition to UV/VIS detection.

Systematic Look at the Affects of Various Solvents, Injection Techniques and Sample Amounts in Preparative HPLC ColumnsData is presented documenting the affects elicited in preparative chromatography under various conditions.

A Totally Integrated 2D System Incorporating Ion-exchange and RP-HPLC for Natural and Biological ProductsAn automated two-dimensional application that incorporates ion-exchange and reversed-phase chromatography for the separation of complex mixtures. Data is presented that shows the usefulness of two dimensional chromatography and implementing alternative chromatography modes.

Novel Approach for Screening of Drug Absorption via an Automated SystemThe system presented provides a faster alternative to other screening methods, as well as an inexpensive analysis estimate of drug permeability for hundreds of drug candidates in a fraction of the time of conventional screening methods.

Preparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected FractionsParameters discussed are column performance, mobile phase constituents, detector settings and collection parameters such as collection via level versus slope and delay volumes. The data offers guidelines for all chromatographers purifying compounds by preparative HPLC and can be implemented into existing systems.

Optimized Sample Load Capacity and Separation Through a Series of Short Preparative ColumnsBy placing the smaller preparative columns in series, loading capacity and separations of compounds can increase with short run times and semi-preparative flow rates, minimizing the overall cost of the larger preparative columns and channeling within the column.

“Train Keep a Rolling and Isolation Gradients”: A Method for Purifying a Large Quantity of Sample via Continuous Injection and Simultaneous Fraction Collection on Multiple FC BedsTrain injection employs continuous injection of sample under isocratic conditions and continuous fraction collection. In addition, isolation gradients also offer an efficient purification of preparative samples when the train injection technique is not applicable, as is the case with complicated chromatograms.

Separation of Flavonoids Through Automated Multiple Column Injections and Sample Concentration via C18 Column CaptureIdentical columns are configured in parallel for multiple injections sequenced by the sample peak elution. Sample is concentrated on a separate C18 preparative column, the purified compound is eluted in high organic solvent for ease of dry-down.

Enhanced Recovery of Compound Mixtures by Concurrent Dissection and Concentration of IsolatesA crude separation of a complex mixture into impure isolates. The impure isolates are reintroduced to the column. Front and back sections are cleaved to fractionate uncontaminated compound on separate columns for concentration. Final elution dispenses pure compound in high organic solvent for ease of dry-down.

Liquid Chromatography

Gilson Applications


Solid Phase Extraction continued

Automated Liquid Handling

Comparison of SPE, ILE, SLE Capabilities to Efficiently Extract Organic Compounds via an Automated SystemThere are many sample preparation techniques available, e.g. protein precipitation, liquid-liquid extraction, dilution, solid phase extraction and immobilized liquid extraction. Every technique has its positive and yes, negative aspects, but SPE, ILE and SLE offer the greatest overall benefit based on their mechanism of isolation, media available, various matrices and automation.

The Automated SPE Assay of Fipronil and its Metabolites from Bee PollenFipronil is used as a pesticide on crops that are pollinated by bees. Fipronil’smetabolites are toxic and study of the metabolites have been increasing. Automation of this separation technique would provide laboratories with an efficient method to extract the fipronil and it’s metabolites for analytical analysis. SPE is utilized for this extraction with verification via HPLC.

Isolation and Determination of Flavonoids from Complex via Automated Solid Phase Extraction and UV/ELSD/MS DetectionAutomation allows efficient SPE method optimization. Fractionation targets specific volumes and UV/ELSD/MS allows selectivity and identification of standards and interfering peaks.Flavonoid recovery at 99±1% on all analytes, while SPE of all matrices eliminates interfering peaks.

Optimization of On-line SPE of Biological samples via an Automated System with Trace enrichment (TEC) prior to Analysis via HPLCIncorporating the use of a trace enrichment cartridge (TEC) in conjunction with dilution prior to HPLC analysis eliminates the time consuming step associated with dry down and minimizes the dilution volume that usually causes a reduction in sample analysis. Hence the TEC increases the signal to noise ratio (increased sensitivity), which can be extremely important when analyzing trace analogues or metabolites.

The Automated Extraction of Silymarin Flavanoligans in Milk Thistle SeedAnti-inflammatory and anticarcinogenic properties as well as scavenging abilities make silymarin a likely candidate for further scientific studies. This necessitates SPE extraction and analytical processing of the extract to provide information on its components. Automation of the extraction provides an efficient process to compliment investigation of silymarin flavanolignans.

Ascorbic Acid and Sodium Benzoate in Orange JuiceThe FDA recently tested 84 soft drink products and found that 54 of them had some detectable benzene. And some had levels as high as 79.2 ppb. Federal rules specify less than 5 ppb in drinking water. This application automates the extraction of Sodium benzoate and vitamin C from orange juice and fruit drinks for analytical processing.

Reference

An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thioridazine from PlasmaThis application describes an automated SPE method coupled with HPLC analysis to determine the levels of Chlorpromazine and Thioridazine in human plasma.

An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen in PlasmaThis application describes an automated SPE method coupled with HPLC analysis to determine the various levels of Ibuprofen and Ketoprofen in human plasma.

The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary SupplementsThis application presents the advantages of using an automated SPE method coupled with HPLC, versus manual SPE, to determine the various levels of Ephedra and Ephedra-like compounds in dietary supplements.

Optimization of SPE by an Automated Method Development ProcedureThe system allows for the optimization of SPE process via multiple collection sites and various conditions without manual intervention. Incorporation of a trace enrichment cartridge to concentrate the eluent versus a dry-down step offers advantages to the dry-down bottle neck and analyte degradation.

A Rapid Extraction and Clean-up Procedure for the Determination of Amnesic Shellfish Poison Toxins in ShellfishA rapid sample preparation procedure has been developed via SPE to determine the levels of domoic acid in shellfish. Data supporting this approach is presented along with the automated SPE procedure incorporating trace enrichment cartridges to increase sensitivity of the assay.

Isolation and Determination of Capsaicin and Dihydrocapsaicin from Complex Matrices via Automated SPE and UV/ELS/MS DetectionMatrices tested include dried habanero, topical analgesic cream and pepper spray. The system allows for the extraction procedure to be automatically optimized for each matrix without operator intervention, providing high recovery.

Totally Automated Method for the Determination of Sudan Dyes in Food via On-line Filtration, SPE and HPLC AnalysisThe system presented offers a totally automated approach to the extraction of Sudan dyes from food products. The product is solubilized, filtered and SPE is performed prior to HPLC analysis, at detection limits below the EU of 0.5 – 1 mg/kg.

Optimizing Parameters that Influence Recovery in SPEA totally automated approach to optimize every aspect of an SPE method without manual intervention on an SPE instrument via fractionation, improving recovery and reducing coefficient of variance.

The Automated extraction of Acaricides from Honey via SPEWith the recent decline in hive bee populations this year the investigation of acaricides in the environment have become more evident. One source of these analyses is the quantity of acaricides in honey. Automation of this separation technique would provide laboratories with an efficient method to extract the acaricides for analytical analysis. Bifenthrin and Fipronilare the acaricides extracted. SPE is utilized for this extraction with a separation via HPLC.

Automated SPE Method for the Determination of Cotinine in Biological Fluid This procedure employs SPE, a new polymeric based cation exchange resin that resists drying, shows excellent flow characteristics, greater load capacity and resistance to aggressive washings.

Melamine Determination in Baby Formula via SPE/HPLCThe cleanup procedure for Melamine involves mix mode cation exchange solid phase extraction. Melamine is analyzed using a zwitterionic HILIC LC column. The method limit of quantitation (LOQ) for Melamine was: 100 ppb for liquid formula and 50 ppb for powdered infant formula powder, and CV of 3-10%.

Automated Enrichment and Separation of Bifenthrin Enantiomers via SPE and HPLCThe (-)enantiomer of bifenthrin degrades in field sediments, causing an incremental increase of the (+)enantiomer. These differences in the enantiomers of pesticides have contributed to the investigation of chiral pesticides by the EPA. In this application, we separated enantiomers of bifenthrin by use of both chiral HPLC methodology and SPE enrichment.

The Examination and Automation of GPC, SPE and QuEChERS for Pesticides in Olive OilSeveral techniques for the separation of pesticides from edible oils are used today to investigate the amount of pesticides in these oils. GPC, SPE and QuEChERS can be used to isolate the pesticides that are found in edible oils.

Determination of Veterinary Drug residues in Fish by an Automated SPE-HPLC SystemThis application presents a simple and automated procedure employing SPE to extract and concentrate the residues from fish tissue prior to direct HPLC analysis.

Solid Phase Extraction

Gilson Applications continued

Fully Automated System Employing Disposable Cartridges in Series to Facilitate the Clean-up of Biological SamplesThe system presented in this application is capable of processing large volume samples (25-200 mL range) fast and efficiently. A number of SepPaks® can be linked together, (1 SepPak® for every 75 mL of sample volume). The automated system consists of a series of controllable valves, to access numerous SepPaks® and choices in solvents for the process, a liquid handler is employed to add the samples to the individual SPE and the product is collected via fraction collection events.

A Novel Approach for the Extraction and Concentration of Compounds in Biological Fluids via On-Line Dialysis and EnrichmentOn-line dialysis and trace enrichment prior to analysis offers a unique approach to sample preparation The automated system introduces a biological sample to a dialysis membrane and through positive fluidics separates the drugs/compounds from the matrix and concentrates it onto a trace enrichment cartridge prior to analysis via HPLC.

Quad-Z 215 with Disposable Tips: Implementing the Use of ZipTips® for the Purification of Biological SamplesThis application demonstrates how the Quad-Z 215 automates sample clean-up using ZipTips. The Quad-Z 215 is also capable of completing numerous liquid handling techniques – with or without tips – under varying microplate formats.

A Compact Automated Liquid Handler for Microbatch Protein CrystallographyA system that incorporates fixed and disposable tips on the Quad-Z 215. Independent probe motion and the use of virtual pumps make the system very versatile in the field of protein crystallography via microbatch techniques.

Accessing the E-Gel® 96 System via the Quad-Z 215 with Disposable TipsThis application examines the suitability of the Quad-Z 215 with disposable tips for the delivery of proteins to the wells of the E-Gel 96 gels for electrophoresis.

Gilson, Inc.

Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for today’s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendary Gilson Pipetman® products to a full range of system solutions, Gilson is the element of purification for your laboratory. Gilson’s innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson.

Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications.

Gilson offers complete solutions including the revolutionary control software, TRILUTION®. TRILUTION® LC Software is a complete HPLC software package designed specifically to meet purification needs. TRILUTION® LH Software is designed to make automation of liquid handling and SPE applications simple and dependable.

Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide.

Quality Policy

It is Gilson, Inc.’s policy to promote a total commitment to quality by each individual throughout the

Gilson organization worldwide. It is Gilson’s goal that this commitment to quality will result in

complete customer satisfaction with both Gilson’s products and services.

ISO 9001 Certification

Gilson is committed to quality, and it shows in everything Gilson does – from design to manufacturing,

technical support and training. Due to this commitment to quality and customer satisfaction, Gilson

has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Gilson S.A.S. are both

ISO 9001 certified.

Training

Gilson provides an extensive program of instrument and software training for users. Standard courses as

well as sessions customized to the user’s specific needs are offered at Gilson’s innovative training center or

in the user’s own laboratory. Computer assisted training courses are also available on CD-ROM or the Gilson

on-line education center, www.training.gilson.com.

Gilson, Inc. World Headquarters

3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA

Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451

[email protected] | [email protected] | [email protected] | [email protected] | [email protected]

Gilson S.A.S.

19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE

Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020

ANCE

Automated Liquid Handling continued

Gel Permeation Chromatography (GPC) Clean-up

Gilson Applications continued

Technical Data for the Quad-Z 215 (fixed probes) Dispensing Small Volumes of DMSOThis study determined the accuracy and CVs for the Quad-Z 215 (fixed probes) for low volume dispensing of DMSO. The results of the testing show the Quad-Z 215 is capable of transferring low volumes of DMSO from a 96-well format into an empty, flat-bottomed 96-well microplate.

Quad-Z 215 with Tips: Volume transfer DataData presented for low volume transfers with tips

Reformatting Procedures Employing a Quad-Z 215 with Disposable TipsThe volumetric accuracy and precision data presented show that the Quad-Z 215 with disposable tips offers researchers the consistency they require in all assays. The use of common tubes and microplates, along with the option of designing a custom rack, provides a high degree of versatility to accommodate variations in procedures.

Determination of Chlorinated Pesticides in Poultry Fat Using GPC and Optional Alumina Clean-up with GC-ECD DetectionPoultry Fat is routinely monitored for the presence of chlorinated pesticides. Gel permeation chromatography (GPC) is a common tool for the post-extraction removal of high molecular weight interferents prior to pesticide analysis. GPC removes these interferents via a size separation mechanism that uses organic solvents and a hydrophobic gel (a cross-linked divinylbenzene-styrene copolymer) to separate the interferents from the lower molecular weight compounds of interest. The interferents are discarded to waste and the fraction containing the pesticides is collected for further clean-up and analysis. GPC clean-up may be followed by additional clean-up procedures such as adsorption chromatography using alumina, Florisil™ or silica.

Increasing Throughput of Gel Permeation Chromatography (GPC) Clean-up via a Parallel Sample Extraction ProcessPost-extraction GPC clean-up is used extensively for preparing food and environmental samples (such as soils) prior to analysis for insecticides, fungicides, herbicides, semi-volatiles, PCBs and other environmental contaminants. GPC clean-up has many benefits, including decreased instrument maintenance costs, higher quality of analytical data, enhanced analytical column life and its capabilities of being automated. One of the disadvantages of GPC clean-up is the relatively slow throughput per sample extract compared to other sample clean-up methods. The purpose of this study was to evaluate the effectiveness of developing an automated GPC clean-up system that is capable of running up to four sample extracts in parallel, along with fraction collection, thus improving sample throughput.

Automated Gel Permeation Chromatography (GPC) Clean-up of Soil Extracts Prior to Analysis for Semivolatile Organic Compound by GC/MC (USEPA Method 8270)The Gilson Automated GX-271 GPC Clean-up System was used to perform the post-extraction clean-up of soil extracts prior

to analysis for semivolatile organic compounds. Semivolatiles were extracted from soils using pressurized fluid extraction (USEPA Method 3545). The extracts were then purified using GPC Clean-up (USEPA method 3640A). Analysis was performed by GC/MS. Recoveries for a variety of semivolatiles were determined.

The Examination and Automation of GPC, SPE and QuEChERS for Pesticides in Olive OilSeveral techniques for the separation of pesticides from edible oils are used today to investigate the amount of pesticides in these oils. GPC, SPE and QuEChERS can be used to isolate the pesticides that are found in edible oils. Each technique provides strengths for its separation of esticides. GPC has the ability to process large amounts of sample, SPE provides disposable cartridges with numerous sorbents to provide separation of the analyte from the matrix, and QuEChERS involves uncomplicated sample cleanup of pesticides in aqueous matrices. This application investigates each of these separation techniques in separating pesticides from oil matrices and presents detailed information on the automation of each separation system.

Increasing Throughput of GPC Clean-up via a parallel Sample extraction process, collection Window Determination and Individual collection per ColumnThe purpose of this study was to evaluate the effectiveness of developing an automated GPC clean-up system that is capable of running 2-4 individual sample extracts in parallel, along with fraction collection per column, thus improving sample throughput. Data is presented using a USEPA GPC calibration standard to determine the time window for fraction collection. A very simple acquisition software program is used to determine this collection window. One established unique food/environmental samples can be injected simultaneously onto the individual stainless steel GPC columns with independent fraction collection per column.

Reference

gilson product guide

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