fungus project wur

Download Fungus project WUR

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  • iGEM 2011 project proposal Wageningen UR 2011Moldly going where no man has gone beforeMold Track `n Trace

  • Following a signal (eGFP) through a hyphaDiffusion of signal carrier through septaA. nidulans

  • Project - Hyphae

  • Initial stimulationProject - Hyphae

  • Initial stimulationFirst signal producedCarrier buildupProject - Hyphae

  • Initial stimulationFirst signal producedCarrier buildupCarrier diffusesProcess start again in cell N+1Project - Hyphae

  • Initial stimulationFirst signal producedCarrier buildupCarrier diffusesProcess start again in cell N+1etc.Project - Hyphae

  • Project - Colony

  • Project - Colony

  • Project - Colony

  • Project - Colony

  • Project - Colony

  • Project - Colony

  • Proposed mechanismsXlnR*eGFP-SvacxpnaxpnaXlnR*XlnR*XlnR*XlnR*XlnR*XlnR*XlnR*

  • Proposed mechanismsGAL4eGFP-SvacUASgal4GAL4UASgal4

  • 3cGAL4 regulation44444410bp10bp8080808080803c3c3c

  • 3c3cCombination448080XlnR*XpnAXpnAGAL3ceGFP-Svac3c

  • S.W.O.T

  • New model organism, A. nidulansVisual signal (GFP)System can be subdivided (modular)Proposed RR have been used beforeSome are even BioBrickedIn house knowledge about A. nidulans

    S.C.O.T

  • Keeping noise from ruining systemTuning the systemLots of constructs need to be made

    S.C.O.T

  • Tuning and noise reduction using the GAL systemOpportunity to gain fundamental knowledge about A. nidulans hyphaeOpportunity to broaden the scope of synthetic biology BioBricksS.C.O.T

  • Long period from transformation until measuring of results, 1 Month.Unknown synthetic territoryEukaryote chassis

    S.C.O.T

  • ?Questions