iGEM 2011 project proposal – Wageningen UR 2011

Mold’ly going where no man has gone before

Mold Track `n Trace

• Following a signal (eGFP) through a hypha

• Diffusion of signal carrier through septa

A. nidulans

Project - Hyphae

• Initial stimulation

Project - Hyphae

• Initial stimulation• First signal produced• Carrier buildup

Project - Hyphae

• Initial stimulation• First signal produced• Carrier buildup• Carrier diffuses• Process start again in

cell N+1

Project - Hyphae

• Initial stimulation• First signal produced• Carrier buildup• Carrier diffuses• Process start again in

cell N+1• etc.

Project - Hyphae

Project - Colony

Project - Colony

Project - Colony

Project - Colony

Project - Colony

Project - Colony

Proposed mechanisms

XlnR*

eGFP-Svac

xpna

xpnaXlnR*

XlnR* XlnR*XlnR*XlnR*XlnR*XlnR*

Proposed mechanisms

GAL4

eGFP-Svac

UASgal4

GAL4

UASgal4

3c

GAL4 regulation

4 4 4 44 4

10bp 10bp

80 80 80 80 80 80

3c 3c

3c

3c

3c

Combination

4 480 80

XlnR*

XpnA

XpnA

GAL3c

eGFP-Svac

3c

S.W.O.T

• New model organism, A. nidulans• Visual signal (GFP)• System can be subdivided (modular)• Proposed RR have been used before• Some are even BioBricked• In house knowledge about A. nidulans

S.C.O.T

• Keeping noise from ruining system• Tuning the system• Lots of constructs need to be made

S.C.O.T

• Tuning and noise reduction using the GAL system

• Opportunity to gain fundamental knowledge about A. nidulans hyphae

• Opportunity to broaden the scope of synthetic biology BioBricks

S.C.O.T

• Long period from transformation until measuring of results, 1 Month.

• Unknown synthetic territory• Eukaryote chassis

S.C.O.T

?Questions