Final Presentation: PowerPoint File

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<ul><li> 1. Dragonflies Feeding on Amphipods; Size Does Matter Linda White, Kenny Danner, Shane Murnan, Craig Henson, and Lori Espieg Research Scientist: John White</li></ul> <p> 2. Introducingour study subject</p> <ul><li>We will be studying theHylellaamphipod. </li></ul> <p> 3. Introducingour prey </p> <ul><li>Dragonfly nymph </li></ul> <p> 4. Step 1 </p> <ul><li>We added 400 mL of non-chlorinated well water to fifteen individual sandwich sized 900 mL quart serving containers .</li></ul> <p> 5. Step 2 </p> <ul><li>We placed four four inch segments of ambulia plastic aquarium plants.</li></ul> <p> 6. Step 3 </p> <ul><li>All of the containers were left overnight to reach ambient temperature and oxygenate the water.</li></ul> <p> 7. Step 4 </p> <ul><li>Dragonfly larvae were separated into individual plastic beverage containers overnight to rest.Dragonfly larvae were not fed to ensure that each dragonfly had an appetite. </li></ul> <p> 8. Step 5 </p> <ul><li>We separated all of the amphipods into four categories; small, medium, large, and females with broods. The females with broods were placed in a separate container. </li></ul> <p> 9. Step 6 </p> <ul><li>We placed eight small, eight medium, and eight large amphipods into each container. </li></ul> <p> 10. Step 7 </p> <ul><li>After allowing the amphipods to rest in the containers, we placed one dragonfly larvae in each container.</li></ul> <p> 11. Step 8 </p> <ul><li>After allowing nature to take its course, we returned to examine our results. </li></ul> <p> 12. 13. Step 9 </p> <ul><li>The dragonfly larvae had a bigger appetite than expected.We found no amphipods.All of our test subjects had been consumed. </li></ul> <p> 14. Step 10 </p> <ul><li>We conducted our experiment a second time using eight of each size amphipod in each arena.We placed the females with broods in a separate container. </li></ul> <p> 15. Step 11 </p> <ul><li>Our dragonfly larvae were left in the arenas for twelve hours.After twelve hours the dragonfly larvae were separated from the amphipods.</li></ul> <p> 16. Step 12 </p> <ul><li>We placed the remaining amphipods and dragonfly larvae in a vial with ethanol solution killing juice to preserve them for measuring. </li></ul> <p> 17. Step 13 </p> <ul><li>We sorted the amphipods based on size remaining on Petri dishes for each arena. </li></ul> <p> 18. Step 14 </p> <ul><li>Using a microscope, we measured the head length of each of the remaining amphipods. </li></ul> <ul><li>We measured the head width of each dragonfly larvae. </li></ul> <p> 19. Step 15 </p> <ul><li>All of our data was entered into a spreadsheet so we could draw conclusions based on our data. </li></ul> <p> 20. Results </p> <ul><li>We determined that the dragonfly larvae preferred the medium size amphipods to the small or large amphipods. </li></ul> <p> 21. 22. Conclusions </p> <ul><li>Through the analysis of our data, we determined that the dragonfly larvae prefer amphipods that are medium sized.</li></ul> <ul><li>Reasons for this could be the energy used to catch the amphipod in relation to the energy gained by consuming the amphipod. </li></ul> <p> 23. Ideas for Future Study </p> <ul><li>Comparing females with and without broods to males of the same size. </li></ul> <ul><li>Comparing females with and without broods. </li></ul> <ul><li>Increase the variation of dragonfly larvae size. </li></ul> <p> 24. More Ideas for Future Study </p> <ul><li>Painting black dots on males to determine if it increases visibility or attractiveness. </li></ul> <ul><li>Analyze energy content to determine if females with broods provide a higher energy return for dragonfly larvae. </li></ul> <ul><li>Introduce other predators to determine which size amphipod they prefer. </li></ul> <p> 25. Potential Predators </p> <ul><li>Toe Biters </li></ul> <ul><li>Crawfish </li></ul> <ul><li>Tadpoles </li></ul> <ul><li>Fish </li></ul> <p> 26. Disclaimer 27. </p> <ul><li>Questions? </li></ul>