Field training September 5th, 2014

Highly Confidential and Embargoed

What do all of these technologies have in common?

Launch Objectives

1. REVITALIZE AND GROW THE STRATAGENE-AGILENT QPCR CUSTOMER BASE.

Revitalize: penetrate Stratagene base which needs an upgrade or is not satisfied with their current instrument

Grow: target core lab and service provider section.

Grow : Geographically: US and China

2. IMPROVE CONNECT RATE OF OUR REAGENTS TO THE INSTALLED INSTRUMENT BASE.

Promotions to improve visibility in market: (1) discount with purchase of instrument (2) sponsored research for our newreagents

Evidence driven campaigns: using financial, publications and CRM data to push relevant products to customer.

AGILENT’S COMPLETE PORTFOLIO Reason to believe: validated on over 100,000 samples across 14 assays and different chemistries

Ready, Go! Each instrument is calibrated as it leaves the factory so you have easier first time set up

Blazing Scan times: all channels, 2 seconds or less irrespective of plex number/ optical channels used.

Success is faster than failure: instrument diagnostics most comprehensive in industry

Ultra-fast reagents: SYBR, Probe and (coming soon) HRM

Choices for the long run: instrument’s modular design, intuitive software, multiple chemistries and open platform.

Performance you would expect from a more expensive machine.

More information on the optics -Each cartridge has 8 excitation and emission read points, one for each row, so the motion system only scans left to right, then right to left.

-8 LEDs for excitation in each cartridge, one per row.

-8 Photodiode detectors in each cartridge. Dichromatic mirrors at each of the eight positions reflect emitted light to photodiode.

-Instrument always saves data for all wells and for all the optical cartridges that are inserted.

No compromise optics 8 excitation and emission read points, next generation LEDs

Thermal uniformity and control better than most competitors in this price point

Robust ultra-fast chemistry capture data from the toughest sample types for the challenging alleles.

Why we win…

Unlike the Bio-Rad and Life Technologies solution Aria’s has: (1) more comprehensive instrument health diagnostics to provide the most assurance for successful runs

(2) more agility through our modularity customer has a lower all-around maintenance cost

(3) better results because precision optics, exquisite thermal stability and analytics capture your high quality data.

Who is the target customer

CCore Lab: Academic

• DDominant Application:• NGS library quant.• Gene expression• Genotyping• MMajor Concerns• Timelines• Cost• Instrument failure• Prestige• SSelling Tactics• Promotions• Cross selling• Application breadth

Core Lab Service Provider 2 industry

• DDominant Applications• CT Patient stratification• Multiplexing assays• Gene expression• Pathogen detection• MMajor Concerns• Timelines• Training• Tracking & compliance• Predictable workflow• SSelling Tactics• Platform stability• Quality & Service

Primary Investigator

• DDominant Applications• Genotyping• Multiplexing assays• Gene expression• Methylation• NGS library quant.• MMajor Concerns• Cost• Assay performance• Publications• SSelling Tactics• Application breadth• Promotions

Translational Medicine Investigator

• DDominant Applications• CT Patient stratification• Multiplexing assays• Gene expression• Microarray validation• MMajor Concerns• Training• Tracking & compliance• Predictable workflow• Publications• SSelling Tactics• Application breadth• Cross-selling

NOW

HO

W

Stratagene Loyalists

How we can win

Platform

Temperature

Uniformity (°C)

Roche LightCycler 0.2

Aria MX 0.2 Stratagene Mx4000 0.25 Stratagene Mx3000P 0.25 Stratagene Mx3005P 0.25 Bio-Rad CFX96 0.4 BioRad MiniOpticon 0.4 BioRad MyiQ 0.4 BioRad Opticon 2 0.4 BioRad Chromo4 0.4 BioRad iQ5 0.4 ABI 7900HT Fast 0.5 ABI 7300 0.5 ABI 7500 0.5 ABI 7500 Fast 0.5 ABI StepOne 0.5

Platform Max. heating/

cooling rate (°C/sec) Roche LightCycler 20.0/20.0 AriaMx 6.0/4.5 Bio-Rad CFX96 5.0/3.3 Roche LightCycler 480 4.8 /2.5 ABI 7500 Fast 3.5/3.5 Bio-Rad MyiQ 3.3/2.0 Bio-Rad iQ5 3.3/2.0

ABI 7900HT Fast 3.0/3.0

Bio-Rad Opticon 2 3.0/2.0 Bio-Rad Chromo4 3.0/2.0

Stratagene Mx3000P 2.5/2.5 Stratagene Mx3005P 2.5/2.5 BioRad MiniOpticon 2.5/2.0 ABI StepOne 2.2/2.2 Stratagene Mx4000 2.2/2.2

ABI 7300 1.1/1.1 ABI 7500 0.8/1.6

Platform Temperature

Accuracy (°C) Aria MX 0.2 or better Roche LightCycler 480 0.2 Bio-Rad CFX96 0.2 ABI 7900HT Fast 0.25 ABI 7300 0.25 ABI 7500 0.25 ABI 7500 Fast 0.25 ABI StepOne 0.25 Stratagene Mx4000 0.25 Stratagene Mx3000P 0.25 Stratagene Mx3005P 0.25 Bio-Rad MyiQ 0.3 Bio-Rad Chromo4 0.3 Bio-Rad iQ5 0.3 Roche LightCycler 0.4 BioRad Opticon 2 0.4

Avoid the mistakes that cause mountains of work

How to beat the competition: Agility, Precision, Speed

Product Group COMPETITION

Life Tech Roche Bio-Rad ILMN

INSTRUMENTS

Limited temperaturerange

Well to well thermalvariability (edge effects)

CCD Camera has lessresolution than Aria

HEAVY & BIG machines

Locked in reagents andinstrumentation

Multiplex tops outat 4

Limited temperature range

Long ramp rate

Thermal precision: accuracy &Uniformity

CCD Camera instruments haveless resolution than our Optics

LED instruments are lastgeneration

Locked in choice, sameamount of money

Less value for your money

H A R V E S T

Exited business

TRADE UP

SOFTWARE

Few protocols

Difficult programing

Least protocols

Difficult programing

Biggest opportunities

The Busy PI: Too many conflicting priorities Avoid the mistakes that cause mountains of work

Bias in NGS runs

Missing Alleles in Gene Expression and Genotyping arrays

Missing alleles in competitor qPCR runs

Complex reporting and software

Track experiments remotely

PAIN To Overcome The Busy PI SECTOR SOLUTION

Principal investigator AriaMx and Agilent Reagents

REASONS Is it because today you need to:

IMPACT Is your current solution causing concerns:

CAPABILITIES What if?, Would it help?

You need to run more NGS runs You don’t have the mental bandwidth?

Consortium: is there batch variation? Is therebias?Technician: will my run fail?Bioinformatician: Did SNPs/variants fall out?

When: running an NGS run it was easy and cost effect to confirm your results Who: Consortiums What: could have more confidence in results especially from new PIs.

You can’t afford the time because you are busy running gene expression or genotyping arrays?

•Director: Did I get consistent results on the hard toquery genes?

•Bioinformatician: Are the algorithms biasedtowards western european populations, so am Imissing alleles?

•Technician: The results don’t seem right, is mytechnique contributing to bias or something else?

When: you had a fast way to ensure consistency of your array workflows Who: The Technician, The Manager, The Bioinformatician What: could have more confidence in the results

My current solution is good enough? •Customer: Did you capture all the information from

my sample?•Manager: Is our chemistry helping us, or hindering?•PI: Can our costs be reduced?•PI: Can I cherry pick (best-in-class) chemistries todevelop new applications?

•Technician: I don’t have time to learn a new system

When: you can resolve difficult loci faster and more reliably with better chemistries? You had fewer repeats because your system was always running at top performance Who: The Technician, The Manager What: T: could be more confident in results and workflow

How to order G8830A

Single Filter Bundle 2 fixed filter 3 fixed filter 6 fixed filter

All prices are at list and in USD All include 2 year warranty

4 fixed filter $27,500

G8830-64000

5 fixed filter $30,000

G8830-64010

Trade UP your instrument (competitor or Agilent) 30% off Promo Code: 9399

How to order G8830A It is as easy as 1, 2, 3 We modeled the ordering process after 3000Mx

1.) You will always need the base unit

G8830A $18,000 list

2.) Choose the number of filters: Fixed choice 5 fixed filters: G8330-64000

4 fixed filters: G8330-64010

3.) Now choose your services List price includes: Option Add: 2 year warranty

Things you need to know: I &F optional delete. First 100 units, included

Step 3: Services affiliated with G8330A

Maintenance Services Preventative Maintenance

Enhanced PM Repair and Bundled Service Installation and Familiarization

Extended Warranty Advantage Silver Bench Services Return to Agilent

Return to Agilent w/Loaner Instrument Exchange

Scott Marzano to discuss in further detail We will send a screen shot cheat sheet in launch packet

How to beat the competition: Agility, Precision, Speed

Product Group COMPETITION

Life Tech Roche Bio-Rad ILMN

INSTRUMENTS

Limited temperaturerange

Well to well thermalvariability (edge effects)

CCD Camera has lessresolution than Aria

HEAVY & BIG machines

Locked in reagents andinstrumentation

Multiplex tops outat 4

Limited temperature range

Long ramp rate

Thermal precision: accuracy &Uniformity

CCD Camera instruments haveless resolution than our Optics

LED instruments are lastgeneration

Locked in choice, sameamount of money

Less value for your money

H A R V E S T

Exited business

TRADE UP

SOFTWARE

Few protocols

Difficult programing

Least protocols

Difficult programing

Biggest opportunities

Helpful Resources

AriaMx Real-Time PCR System Selected Specifications

Description SpecificationExcitation source 8 dye specific LEDs per optical module

Detection 8 silicon photodiodes per optical module

Dyes (at launch) ROX, FAM, HEX, CY3, CY56 slots, swappable cartridges, no reference channel needed

Dye selection Excitation and emission

Electrical power (input) 100-240VAC, 50/60Hz, 1100A

Thermal system Pelletier-based, 96 well

Thermal system temperature range 25.0 – 99.9 CHeating: 6C/SecCooling: 3C/secAccuracy: ± 0.2°C or better of target

Cycling speeds 40 cycles in 43 minutes

Operating environment 18-35C (65-95F), 85% non-condensing humidity7500 feet, max altitude

Weight 50 lbs. (23 kg)

Dimensions 19.7” W x 18.1 “D x 16.5” H (50cm x 46cm x 42cm)

Warranty & Reliability Two year warrantyNo install plate necessaryOn-board QC testing system: long version: 125 pain points, Short version: 65 pain points

Sample containers 0.2ml tubes, 96 well plates, strip tubes