facs diva training garvan mlc flow cytometry facility
DESCRIPTION
Garvan MLC flow Cytometry Facility guide to using Facs DiVaTRANSCRIPT
MLC Flow Cytometry Facility
BD FACS DiVa
Training
Flow Cytometry
Login
• Windows Login • User - Administrator
• Password – BDIS or bdis
• DiVa Login• Username - 3 letters first name + 3 letters last name
• Password – as user name
YOUR DISCIPLINE HERE
DiVa Overview
DiVa Overview
Browser
Window
DiVa Overview
Browser
Window
Cytometer
Window
DiVa Overview
Browser
Window
Cytometer
Window
Worksheet
Window
DiVa Overview
Browser
Window
Cytometer
Window
Worksheet
Window
Acquisition
Dashboard
DiVa Overview
Browser
Window
Cytometer
Window
Worksheet
Window
Acquisition
Dashboard
Inspector
DiVa Overview
Browser
Window
Cytometer
Window
Worksheet
Window
Acquisition
Dashboard
Inspector
DiVa Overview
Browser
Window
Cytometer
Window
Worksheet
Window
Acquisition
Dashboard
Inspector
File menu
DiVa Windows -Browser
Organises
data in a
hierarchical
and ordered
manner
DiVa Windows - Browser
DiVa Windows - Browser
DiVa Windows - Cytometer
Status - instrument errors
( turns red)
Parameters – controls PMT
amplification and signal
collection
Threshold – controls triggering
Laser – Laser delays and
scaling
Compensation – acquisition
defined compensation
matrix
Ratio - ???
DiVa Windows - Cytometer
DiVa Windows -Cytometer
Parameter
name
DiVa Windows - Cytometer
DiVa Windows - Cytometer
DiVa Windows - Cytometer
Worksheets• Global worksheet
• Is not linked to specific data source
• Reloads data on tube selection
• Used when batching
• Different global worksheets can be linked to different
specimens
• Sheet • Is linked to data
• Does not refresh upon tube selection
• Compensation • Is linked to compensation tubes
• Must be used to perform acquisition compensation
Worksheets
Visualising Signal Data
Dot
plot
Histogram
Density
plot
Contour
plot
Statistical
measures are
also used to
identify
changes
DiVa Windows - Acquisition
Dashboard
DiVa Windows - Acquisition
Dashboard
DiVa Windows - Acquisition
Dashboard
DiVa Windows - Acquisition
Dashboard
DiVa Windows – Inspector
DiVa Windows – Inspector
DiVa Windows – Inspector
DiVa Windows – Inspector
Creating a New Experiment
Creating a New Experiment
Creating a New Experiment
Compensation Workflow
Compensation Workflow
Compensation Workflow
Using the Layout editor
Using the Layout editor
Using the Layout editor
Fluidics Startup/Shutdown
Canto I, Canto II and Aria only
Fluidics Startup/Shutdown
Canto I, Canto II and Aria only
Fluidics Startup/Shutdown
Canto I, Canto II and Aria only
Acquiring data
• Activate tube to collect data
• Hit acquire
• Check stopping gate and numbers
• Hit record
• Hit remove tube
• Remove tube and flick aspirator arm to left
• repeate
Exporting Data
Exporting Data
Exporting Data
Cleaning Instrument
1. Run 10 % fresh bleach soln. – 5 mins*
2. Run 4 % extran – 5 mins
3. Run distilled water – 5 mins
4. Shutdown if no other users in the day
* For Biohazardous samples
Troubleshooting
Troubleshooting