EXPERIMENTAL PATHWAYSEXPERIMENTAL PATHWAYS

1.What’s the Cyclins?1.What’s the Cyclins?

2.What’ the relationship between 2.What’ the relationship between cyclins and MPF?cyclins and MPF?

What’s the cyclins?What’s the cyclins?

一、 一、 MPFMPF 卵细胞促成熟因子，卵细胞促成熟因子， matuation-promotimatuation-promoti

ng factorng factor ；；细胞促分裂因子，细胞促分裂因子， mitosis-promoting factmitosis-promoting fact

oror ；；MM 期促进因子，期促进因子， M-phase-promoting factM-phase-promoting fact

oror ）。 ）。

二、二、 Cyclins Cyclins 细胞周期素细胞周期素

yeast: Cln1,Cln2,Clin3,Clb1-Clb6yeast: Cln1,Cln2,Clin3,Clb1-Clb6

Vertabrate: A1-2Vertabrate: A1-2 、、 B1-3 B1-3 、、 CC 、 、 D1- 3D1- 3 、、E1-2E1-2 、、 EE 、、 FF 、、 GG 、、 HH 等等

Every cyclins has a 100 amides keep sequence, which called cyclin-frame.It can interpose cyclin combine with CDK(cycling dependent kinase), different cyclins will recognize different CDK to make a cycling-complex.

M-

周期蛋白激酶周期蛋白激酶

CDK ( cyclin dependent kinase),

Different cycling expression in different time and combine with different CDK to accommodate different CDKase activition.

注射实验表明：注射实验表明： 1.1. 孕酮诱导卵母细胞成熟；孕酮诱导卵母细胞成熟； 2.2. 成熟卵细胞质中，含有卵母细胞成熟 成熟卵细胞质中，含有卵母细胞成熟 的因子，称做 的因子，称做 MPFMPF

P619P619

Kinetics of Kinetics of Xenopus Xenopus oocyte oocyte activation by activation by progesterone progesterone and cyclinA and cyclinA mRNAmRNA

GVBD%

What’s the relationship between What’s the relationship between cycling and MPF?cycling and MPF?

cyclinA is an activator of MPFcyclinA is an activator of MPF

MPF=CDC2+Cyclin B =a complexMPF=CDC2+Cyclin B =a complex

MPF

Cyclin BCyclin B 的降解途径 的降解途径

P34cdc2

The yeast protein was called The yeast protein was called p34cdc2 or simply cdc2.p34cdc2 or simply cdc2.

The first evidence of a link between The first evidence of a link between cdc2 and MPF came as the result of a cdc2 and MPF came as the result of a collaboration between yeast and collaboration between yeast and amphibian research groupamphibian research group

Recall from fission yeast were shown to react specifically with the 32- and 45-kDa protein

Antibodies formed against cdc2 from fission yeast were shown to react specifically with the 32-kDa component of MPF isolated from Xenpus eggs.

This component of MPF is a homologue of the 34-kDa yeast kinase and, the machinery controlling the cell cycle in yeast and vertebrates contains evolutionarily conserved components.

2001 年 10 月 8 日 , L. Hartwell 、 P. Nurse 、 T. Hunt 因对细胞周期调控机理的研究而荣获诺贝

尔生理医学奖。