era7 bacterial genome annotation

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BACTERIAL GENOME ANNOTATION SERVICE

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Page 1: Era7 Bacterial Genome Annotation

BACTERIAL GENOME ANNOTATION

SERVICE

Page 2: Era7 Bacterial Genome Annotation

www.era7bioinformatics.com

Page 3: Era7 Bacterial Genome Annotation

Specifically designed for data

Our service highlights

Based on

www.era7bioinformatics.com

So it’s and

It’s according to our customer needs

Page 4: Era7 Bacterial Genome Annotation

specifically designed for data

www.era7bioinformatics.com

Page 5: Era7 Bacterial Genome Annotation

specifically designed for data

www.era7bioinformatics.com

Page 6: Era7 Bacterial Genome Annotation

specifically designed for data

www.era7bioinformatics.com

Page 7: Era7 Bacterial Genome Annotation

specifically designed for data

www.era7bioinformatics.com

Page 8: Era7 Bacterial Genome Annotation

specifically designed for data

Error-tolerant system

It can handle sequencing errors that may cause:

www.era7bioinformatics.com

Indels, Frameshifts, Base substitution

Page 9: Era7 Bacterial Genome Annotation

specifically designed for data

Error-tolerant system

It can handle sequencing errors that may cause:

www.era7bioinformatics.com

Works with

Large number of contigs: There’s no need to have the genome closed

Large Bacterial Genomes: Up to 7Mb genomes annotated successfully

Indels, Frameshifts, Base substitution

Page 10: Era7 Bacterial Genome Annotation

We use

So our service is

In 24 hours you can have your bacterial genome analyzed

www.era7bioinformatics.com

In 24 hours you can have your bacterial genome analyzed

Page 11: Era7 Bacterial Genome Annotation

We use

So our service is

In 24 hours you can have your bacterial genome analyzed

www.era7bioinformatics.com

In 24 hours you can have your bacterial genome analyzed

and

whether you need to annotate 1 genome or 100 we can handle it

Page 12: Era7 Bacterial Genome Annotation

It’s

The annotation can be focused on particular aspects you may be interested in:

www.era7bioinformatics.com

may be interested in:

The presence of a particular enzime activity

Resistance to antibiotics

Mobile elements…

Page 13: Era7 Bacterial Genome Annotation

annotation

The annotation system is based on the latest releases of

www.era7bioinformatics.com

Uniprot and GenBank databases

So you’ll get an up to date annotation

Page 14: Era7 Bacterial Genome Annotation

www.era7bioinformatics.com

Page 15: Era7 Bacterial Genome Annotation

Sequencing data

www.era7bioinformatics.com

Sequencing data

Page 16: Era7 Bacterial Genome Annotation

Sequencing data

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Page 17: Era7 Bacterial Genome Annotation

Sequencing data

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

Page 18: Era7 Bacterial Genome Annotation

Sequencing data

Manual selection by an expert in bacterial

genomics

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

Page 19: Era7 Bacterial Genome Annotation

Sequencing data

Manual selection by an expert in bacterial

genomics

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

BG7

Page 20: Era7 Bacterial Genome Annotation

Sequencing data

Preliminaryresults

Manual selection by an expert in bacterial

genomics

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

BG7

Page 21: Era7 Bacterial Genome Annotation

Sequencing data

Preliminaryresults

Manual selection by an expert in bacterial

genomics

AutomatedQC Tests

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

BG7Results

Passing QC tests

Page 22: Era7 Bacterial Genome Annotation

Sequencing data

Preliminaryresults

Manual selection by an expert in bacterial

genomics

AutomatedQC Tests

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

BG7Results

Passing QC tests

Definitiveresults

Page 23: Era7 Bacterial Genome Annotation

Sequencing data

Preliminaryresults

Manual selection by an expert in bacterial

genomics

AutomatedQC Tests

An expert in

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

Reference Proteinsand RNAs

genomics

BG7Results

Passing QC tests

Definitiveresults

An expert in Bacterial genomics checks that everything is

OK

Page 24: Era7 Bacterial Genome Annotation

Sequencing data

Preliminaryresults

Manual selection by an expert in bacterial

genomics

AutomatedQC Tests

An expert in

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

24 hrReference Proteinsand RNAs

genomics

BG7Results

Passing QC tests

Definitiveresults

An expert in Bacterial genomics checks that everything is

OK

Page 25: Era7 Bacterial Genome Annotation

AutomatedQC Tests

Sequencing data

Preliminaryresults

An expert in

Manual selection by an expert in bacterial

genomics

www.era7bioinformatics.com

Sequencing data

Info about yourproject, your needs

24 hrReference Proteinsand RNAs

BG7Results

Passing QC tests

Definitiveresults

An expert in Bacterial genomics checks that everything is

OK

genomics

Page 26: Era7 Bacterial Genome Annotation

BG7 System

Input contigs (nt)A

B

C

www.era7bioinformatics.com

Page 27: Era7 Bacterial Genome Annotation

Input contigs (nt)A

B

C

A B C

BG7 System

www.era7bioinformatics.com

Input contigs translated into the 6 reading frames (aa)

Page 28: Era7 Bacterial Genome Annotation

Input contigs (nt)A

B

C

A B C

BG7 System

www.era7bioinformatics.com

Input contigs translated into the 6 reading frames (aa)

Reference Proteins (aa)

Page 29: Era7 Bacterial Genome Annotation

Input contigs (nt)A

B

C

A B C

BG7 System

www.era7bioinformatics.com

Input contigs translated into the 6 reading frames (aa)

Reference Proteins (aa)are searched in the translated contigs

Page 30: Era7 Bacterial Genome Annotation

Genes are defined based on sequencesimilarity to proteins

BG7 System

www.era7bioinformatics.com

A

B

C

Page 31: Era7 Bacterial Genome Annotation

Genes are defined based on sequencesimilarity to proteins

BG7 System

www.era7bioinformatics.com

A

B

C

Page 32: Era7 Bacterial Genome Annotation

RNAs and then searched for in the contigsand annotated

Genes are defined based on sequencesimilarity to proteins

BG7 System

www.era7bioinformatics.com

A

B

C

Page 33: Era7 Bacterial Genome Annotation

RNAs and then searched for in the contigsand annotated

Genes are defined based on sequencesimilarity to proteins

BG7 System

www.era7bioinformatics.com

A

B

C

Page 34: Era7 Bacterial Genome Annotation

What would you get at the end?

A Fast and Accurate genome annotation rich in functional data: GO, Interpro…

Deliverables in several formats:

www.era7bioinformatics.com

Deliverables in several formats:

XML, GenBank, GFF, Fasta, .xls (Excel), .doc (Word), .txt

An user friendly annotation viewer

Page 35: Era7 Bacterial Genome Annotation

And some important things to know:

Working with us means

No IP over the results, they’re all yours

CDA

Bill by project

www.era7bioinformatics.com

Bill by project

Of course, there are other ways…

research collaboration

Page 36: Era7 Bacterial Genome Annotation

Follow us on:

eduardopareja marina_manrique rtobes

Eduardo Pareja Marina Manrique Raquel Tobes

www.era7bioinformatics.com

Marina Manrique Raquel Tobes

Join our NGS group at LinkedIn

[email protected]

[email protected]

[email protected]