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  • plorer®/Eppendorf Xplorer® plusEN)nt

    Register your instrument! www.eppendorf.com/myeppendorf

    Eppendorf Xplorer®/ Eppendorf Xplorer® plus Adjustment

  • Copyright © 2015 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner.

    Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.

    epT.I.P.S.® and Eppendorf Xplorer® are registered trademarks of Eppendorf AG, Germany.

    Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

    Further information can be found on our website www.eppendorf.com.

    All Xplorer and Xplorer plus versions covered by U.S. Patent Nos. 6,499,365; 6,778,917; 7,585,468; 7,674,432; 7,694,592; 8,028,592; 8,096,198; 8,570,029

    All Xplorer and Xplorer plus versions single-channel up to 1000 μL and all multi-channel up to 300 μL additional covered by U.S. Patent Nos. 7,434,484

    All Xplorer and Xplorer plus versions multi-channel up to 300 μL additional covered by U.S. Patent No. 7,673,532

    P-TR-0000302-04/012015

  • 3 Table of contents

    Eppendorf Xplorer®/Eppendorf Xplorer® plus English (EN)

    Table of contents

    1 General information on adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

    2 Change adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Proceed as follows to perform an adjustment for special conditions, or to reset the adjustment to the factory setting.7

    2.1 Pswrd. protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2.2 Factory settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

    2.2.1 Reset to the factory setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.3 Liquid type ethanol 75% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.4 Liquid type glycerol 50% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.5 epT.I.P.S. long . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.6 Altitude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.7 Adjustment by the user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

    2.7.1 1-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.7.2 2-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 2.7.3 3-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

    2.8 Marking the Xplorer pipette to show the adjustment has been changed. . . . . . . . . . . . . . . . . . 17

    3 Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.1 Limiting values for measurement deviations according to ISO 8655 ddd . . . . . . . . . . . . . . . . . 19

    3.1.1 Xplorer pipette, single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.1.2 Xplorer pipette, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

    3.2 Eppendorf AG limiting values for measurement deviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3.2.1 Xplorer pipette, single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3.2.2 Xplorer pipette, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

    4 Speed table Eppendorf Xplorer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 4.1 Dispensing speed of single-channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 4.2 Speed table for Xplorer multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

    5 Volume limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

    6 Additional volumes during aspiration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

  • Table of contents Eppendorf Xplorer®/Eppendorf Xplorer® plus English (EN)

    4

  • 5 General information on adjustment

    Eppendorf Xplorer®/Eppendorf Xplorer® plus English (EN)

    1 General information on adjustment

    The piston stroke of the Xplorer pipettes can be changed in the Adjustment option by the user. This document provides information when you should replace the adjustment Factory settings by a different adjustment and what should be considered when doing so. A selected adjustment can be changed again at any time. It is also possible to reset to the adjustment Factory settings at any time.

    If the adjustment Factory settings is not valid for the Xplorer, this will be displayed by the symbol at the top left in the header in the display.

    The pipette was adjusted and tested before delivery.

    Changing the adjustment is sometimes recommended for solutions which are very different from water with regard to their density, viscosity, surface tension and/or vapor pressure etc. If the density of an aqueous solution changes by approximately ±10%, for example, because of the salt concentration, the volume will change by approximately ±0.2%. This statement does not apply if other relevant properties of the liquid also change.

    If the pipette is used at extremely high altitudes, it must be calibrated to the ambient air pressure. At 1 000 m above sea level, the volume error of a 100 μL pipette is about –0.3%.

    When using tips which are very different to standard tips with regard to their geometry, changing the adjustment can improve the dispensing accuracy (systematic error).

    You can reset a changed adjustment by means of a few simple steps.

    Changes to the adjustment will not affect the dispensing precision (random error). Precision (random error) can be improved by replacing worn parts. Precision (random error) is also strongly influenced by handling.

    This operating manual applies to devices with software version 2.06.00 or higher.

    NOTICE! Incorrect dispensing volume with special liquids and due to temperature differences. Solutions with physical data very different than water or differences in temperature between pipette, pipette tip and liquid can result in faulty dispensing volumes.

     Avoid temperature differences between the pipette, pipette tip and liquid.  Ensure that the temperature is between 20 °C and 27 °C and remains constant at ±0.5 °C.  Check the dispensing volume and make sure that you can affirm all the questions listed in

    the general information.

    Information on the random and systematic errors determined before delivery can be found in the enclosed Eppendorf Certificate. If the factory setting has been replaced by a different adjustment, this renders the validity of the Eppendorf Certificate invalid. If you reset the adjustment to the factory setting, the random and systematic errors determined before delivery and the enclosed Eppendorf Certificate will apply again.

  • General information on adjustment Eppendorf Xplorer®/Eppendorf Xplorer® plus English (EN)

    6

    Before making changes to the adjustment, you must check the existing adjustment in the factory setting with distilled water.

    The actual volume can be checked by weighing:

    The density of distilled water is approx. 0.9982 mg/μL at 20°C and 0.9965 mg/μL at 27°C.

    If the set volume corresponds to the actual volume, no correction is necessary.

    If there is a difference between the actual volume and the set volume of distilled water, please check the following:

    • Is there any liquid dripping from the tip? • Is the pipette tip fitted leak-proof?

    • Is the tip cone undamaged? • Are the piston and the cylinder leak-proof? • Does the temperature of the pipetted liquid correspond to:

    – the temperature of the device? – the ambient air temperature?

    • Is the weighing location free from drafts? • Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid? • Has the correct numerical value for "Density liquids at weighing temperature" been used for the

    calculation of the actual volume? • Is the set volume correct? • For very small volumes (≤ 10 μL): is the fine balance sufficiently sensitive (balance resolution:

    0.001 mg)? • Were original epT.I.P.S pipette tips used for testing?

    No adjustment changes are allowed unless you can answer all the questions with "yes".

    In all other cases, the problems associated with the questions answered with "no" must be eliminated. If the pr

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