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Enzyme Kinetic Zhi Hui

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Page 1: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Enzyme Kinetic

Zhi Hui

Page 2: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Enzyme Kinetics

• Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality• Enzymes are the agents of metabolic function• What we want to be able to determine: – Maximum velocity – Substrate affinity – Inhibitor affinity• What it can tell us: – Flow through metabolic pathways – Utilization of substrates• What can we do with the information: – Control and manipulate metabolic events

Kinetics is the study of the rates of reactions

Page 3: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

The Michaelis-Menten Equation

Page 4: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Important Conclusions of Michaels - Menten Kinetics

• when [S]= KM, the equation reduces to

• when [S] >> KM, the equation reduces to

• when [S] << KM, the equation reduces to

Page 5: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Important Conclusions of Michaels - Menten Kinetics

Page 6: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Lineweaver – Burk Double Reciprocal Plots

• It is difficult to determine Vmax experimentally• The equation for a hyperbola can be transformed into

the equation for a straight line by taking the reciprocal of each side

• The formula for a straight line is y = mx + b

• A plot of 1/V versus 1/[S] will give a straight line with slope of KM/Vmax and y intercept of 1/Vmax

• Such a plot is known as a Lineweaver-Burk double reciprocal plot

Page 7: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Lineweaver – Burk Double Reciprocal Plots

Page 8: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Significance of Km

• Km is a constant• Small Km means tight binding; high Km means weak

binding• Useful to compare Km for different substrates for one

enzyme

Hexokinase : D-fructose – 1.5 mM

D-glucose – 0.15 mM• Useful to compare Km for a common substrate used b

y several enzymes

Hexokinase: D-glucose – 0.15 mM

Glucokinase: D-glucose – 20 mM

Page 9: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Kinetic vs Chemical Mechanism

• An enzyme kinetic mechanism is the order of substrate addition and product release in an enzyme catalyzed reaction

• A chemical mechanism is the chemical pathway of conversion of S → P, including the structures of any intermediates

Page 10: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Bi-substrate Reactions

• The Michaelis –Menten model of enzyme kinetics was derived for single substrate reactions

• The majority of enzymatic reactions have multiple substrates and products

• Bi-substrate reactions account for ~ 60% of the known enzymatic reactions.

Page 11: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Substrate Addition / Product Release

• The order of substrate addition and product release in most enzymatic reactions follow two reaction mechanism

– Sequential reaction - all substrates must bind to the enzyme before the reaction occurs and products are released

• Ordered sequential • Random sequential – Ping-pong reaction - one or more products are relea

sed before all substrates have been added and an alternate stable enzyme form, F, is produced in the half reaction

Page 12: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

1) Sequential Reaction• Ordered sequential

• Random sequential

Page 13: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

2) Ping-pong Reaction

Page 14: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Initial Velocity Plots• sequential reaction exhibits an intersecting pattern of lines Order and random substrate additions cannot be distinguished in this type of plot

• Ping-pong reaction shows

parallel or non- intersecting lines

Page 15: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Influence of enzyme concentration

v = k3 [E], as [S]>>[E]

Page 16: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Influence of temperature

Optimum temperature,most of them are in the range from 35 to 45 .℃

Page 17: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Influence of pH

Optimum pH

Page 18: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Enzyme Inhibition

Enzyme inhibitors are important for a variety of reasons

1) they can be used to gain information about the shape on the enzyme active site and the amino acid residues in the active site.

2) they can be used to gain information about the chemical mechanism.

3) they can be used to gain information about the regulation or control of a metabolic pathway.

4) they can be very important in drug design.

Page 19: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Enzyme Inhibition• Reversible inhibitor: a substance that binds to an en

zyme to inhibit it, but can be released – usually involves formation of non-covalent bonds

– Generally two types

• Dead end

• Product

• Irreversible inhibitor: a substance that causes inhibition that cannot be reversed

– usually involves formation or breaking of covalent

bonds to or on the enzyme

Page 20: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Inhibitors

Irreversible inhibition

Reversible inhibition

competitive inhibition

non-competitive inhibition

uncompetitive inhibition

Page 21: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Irreversible inhibition

• Irreversible inhibition: The inhibitor combine with essential group of

enzyme active center by covalent bond, resulting in enzymatic activity loss.

Page 22: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Inhibition Patterns

• An inhibitor may bind at the same site as one of the substrates

– these inhibitors structurally resemble the substrate

• An inhibitor may bind at an alternate site affecting catalytic activity without affecting substrate binding

• Many inhibitors do both• Most common types – Competitive

– Mixed or Non-competitive

– Uncompetitive

Inhibitors act in a variety of mechanisms

Page 23: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Competitive Inhibition

• Competitive inhibitor competes with a substrate for the enzyme - substrate binding site

Malonate is a

competitive

inhibitor of

succinate for

succinate

dehydrogenase

Page 24: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

• A competitive inhibitor reduces the amount of free enzyme available for substrate binding thus increasing the Km for the substrate

• The effect of a competitive inhibitor can be overcome with high concentrations of the substrate

Competitive Inhibition

Page 25: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Competitive Inhibition

Page 26: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

• Unimolecular

Reaction

• Bimolecular

Reaction

Competitive Inhibition

Page 27: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Uncompetitive Inhibition

• An uncompetitive inhibitor binds to the enzyme substrate complex but not to free enzyme

• The result is a decrease in Vmax and Km

• The effect of an uncompetitive inhibitor can not be overcome by high concentrations of the substrate

Page 28: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Uncompetitive Inhibition

Page 29: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Uncompetitive

Page 30: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Mixed or Non-Competitive Inhibition• The inhibitor can bind to both free enzyme and the ES comple

x

• The affinity of the inhibitor to the two complexes might be different

– If binding of inhibitor changes the affinity for the substrate, Km will be changed and called mixed inhibition

– If only Vmax affected called Non-competitive inhibitor

Page 31: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Mixed Inhibition

Page 32: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

• The result will be decrease in Vmax and either an increase or decrease in Km

• The effect of an non-competitive inhibitor can only be partially overcome by high concentrations of the substrate

Mixed Inhibition

Page 33: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

Non-Competitive

Page 34: Enzyme Kinetic Zhi Hui. Enzyme Kinetics Enzymes endow cells with the remarkable capacity to exert kinetic control over thermodynamic potentiality Enzymes

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