colu

mns

The Acclaim RSLC 2.2 m columns feature a well-balanced integration of high column efficiency, excellent perfor-mance, complementary selectivity, as well as stable and rugged column packing. These columns generate 25 to 50% less backpressure compared to sub-2 m particle columns, and are more resistant to column fouling, making them compatible with both standard and ultrahigh pressure LC instrumentation. These features allow the Acclaim RSLC 2.2 m columns to provide rapid separation solutions for a broad range of applica-tions, including pharmaceutical, food and beverage, environmental, chemical, consumer products, and more.

Column Features Highcolumnefficiency

Easeofuse(lowbackpressureandmoreresistancetocolumnfoulingcomparedtosub-2mcolumns)

Excellentpeakshapeforbasicanalytes

Complementaryselectivity

BroadrangeofapplicationsforRapidSeparationLiquidChroma-tography(RSLC)

Easymethodtransferandaccel-erationusingtheDionexMethodSpeed-UpCalculator

A Well-Balanced SolutionAcclaimRSLC2.2mcolumnsare

basedonspherical,porous,high-puritysilicaparticles,andprovideasimpleandreliablesolutionforRSLC.AcclaimRSLC2.2mcolumnsareavailableinfourchemistries:high-densitymo-nomericC18andC8,Polar-Advantage(PA),apolar-embeddedreversed-phase(RP),andPolarAdvantageII(PA2),anamide-embeddedchemistrywhichhasexcellenthydrolyticstability(pH1.5to10).

Selectivityisthemostimportantfactorinthesuccessofaseparation.Onceselectivityisobtainedbychoos-ingtherightcolumnchemistryandoptimizingchromatographicconditions,higherthroughputorfasteranalysiscanbeachievedbyusingthesamecolumnchemistrywithasmallerparticlesize.However,smallparticle(e.g.,sub-2m)columnsimposesomepracticaldif-ficulties,suchashigherbackpressure

A c c l a i m R S L C C o l u m n s ( 2 . 2 m )

Passion. Power. Productivity.

wesrawlinsThermo

2

(oftenrequiringanultrahighpres-sureLCsystem)andsusceptibilitytocolumnfouling.Columnspackedwith2.02.5mparticlesprovideawell-balancedsolutionforRSLC.Comparedtotheirsub-2mcounterparts,AcclaimRSLCcolumnsfacilitatecompetitiveefficiency,much-reducedbackpres-sure,andbetterresistancetocolumnfouling.Therefore,AcclaimRSLC2.2mcolumnsfeatureawell-balancedintegrationofhighcolumnefficiency,excellentperformance,complementaryselectivity,aswellasstableandruggedcolumnpacking.

High Column Efficiency with Low Backpressure

Becauseoftheuseofsphericalsilicaparticlesofuniformsize,ad-vancedsurfacemodificationprocess,andoptimizedultra-high-pressurepackingmethod,AcclaimRSLC2.2mcolumnsprovidehighefficiency(Figure1)atsignificantlyreducedback-pressure25to50%lowercomparedtosub-2mparticlecolumns.ThecombinationofhighefficiencyandlowbackpressuremakesAcclaimRSLC2.2mcolumnsexcellentchoicesforhigh-throughputapplicationsonbothultrahighpressure(>500bar)andstandardpressure(

3

Broad Range of Applications

Bisphenol-A Diglycidyl Ether (BADGE) and Related Impurities

BADGEisawidely-usedepoxymonomerderivedfrombisphenol-A.Becauseepoxiesarewidelyusedinfood-contactapplications,theresiduesofthisgroupofsubstances(Figure4)areofconcerntopublichealthofficials.

Figure5demonstratesthatastan-dardAcclaim120C18column(5m,4.6150mm)canbaselineseparateallsevenanalytesofinterestina20mingradientmethod.Aftertransferringthemethodtothe2.2m,2.150mmRSLCC18column,thesameseparationcanbeachievedwithin3minasix-foldaccelerationwithonly8%mobilephaseconsumption.

Figure 4. Structures of bisphenol-A diglycidyl ether (BADGE) and related impurities.

Figure 5. Separation of bisphenol-A diglycidyl ether (BADGE) and related impurities using A) Acclaim RSLC C18 2.2 m and B) Acclaim 120 C18 5 m columns.

25397

Compound R R'

OR'

RO

Bisphenol-A H H

BADGEO O

BADGE + H2O OHOH

O

BADGE + HCl OHCl

O

BADGE + 2H2O OHOH

OHOH

BADGE + 2HCl OHCl

OHCl

BADGE + HCl + H2O OHOH

OHCl

Peak

1

2

3

4

5

6

7

0 5 10 15 20

0

125

mAU

Minutes

1

2

3 56 7

R1,2 = 2.69R1,2 = 4.55

Column: A: Acclaim120 C18, 5 m, 4.6 150 mm B: Acclaim RSLC C18, 2.2 m, 2.1 50 mmMobile Phases: (A) Water (B) Methanol

Program A: Time (min): 10.0 0.0 15.0 20.0 %A 50 50 20 20 %B 50 50 80 80 Flow: 1.20 mL/min, Backpressure: 2300 psi

Program B: Time (min): 2.5 0.0 2.6 3.5 %A 50 50 20 20 %B 50 50 80 80 Flow: 0.49 mL/min, Backpressure: 4300 psi

B

A

Temperature: 40 CInjection: A: 30 L B: 3.6 LDetector: UV, 277 nm, A: 2 Hz data rate B: 10 Hz data rate

Peaks: (50 g/mL each) 1. BADGE + 2H2O 2. Bisphenol-A 3. BADGE + H2O 4. BADGE + HCl + H2O 5. BADGE 6. BADGE + HCl 7. BADGE + 2HCl

4

25398

4

Separation of Vanilla ExtractOfthe100orsoflavorcomponents

innaturalvanilla,fourmajorcompoundsareroutinelyexaminedasameasureofquality(Figure6).AOACOfficialMethod990.25isawidelyusedfordeterminingvanillaextractquality,butspecifiesatypeofcolumnthathasonlyabout7000plates,andtakesoveranhourtorun.TheAcclaimRSLCcolumnhasthesamenumberofplates,betterselectivity,andrunsinminutes.Usingwell-knowngeometricscalingrules,theassaycanbeacceleratedsixfold.Doublingtheflowrateacceleratestheanalysis12-fold,withnosacrificeinthequalityofthechromatogram.Thisac-celerationisillustratedinFigure7.

Figure 6. Structures of vanilla components.

Figure 7. Accelerated assay of vanilla extract using A) Acclaim C18 5 m and B and C) Acclaim RSLC 120 C18 2.2 m columns.

Compound R R'p-Hydroxybenzoic acid CO2H Hp-Hydroxybenzaldehyde CHO HVanillic acid CO2H OCH3Vanillin CHO OCH3

25399

R'HO

R Peak

1234

Column: A: Acclaim 120 C18, 5 m, 4.6 150 mm B, C: Acclaim RSLC C18, 2.2 m, 2.1 50 mmMobile Phase: 200 mM HOAc in 10% (v/v) CH3OHFlow: A: 1.00 mL/min B: 0.41 mL/min C: 0.82 mL/minBackpressure: A: 1900 psi B: 3100 psi C: 5830 psiTemperature: 20 C

Injection: A: 10 L B: 1.2 L C: 1.2 LDetector: UV, 254 nm, A: 1 Hz data rate B: 5 Hz data rate C: 10 Hz data ratePeaks: 1. p-Hydroxybenzoic acid 2. p-Hydroxybenzaldehyde 3. Vanillic acid 4. Vanillin Sample: Commercial vanilla extract in 40% ethanol, filteredReference: AOAC Official Method 990.25

25400

0 10 20 30 40 50 60

0

160

mAU

A

1 23

4

0 2 4 6 8 10

0

250B

1 23

4

0 1 2 3 4 5

0

220

Minutes

C

1 23

4

Standard Column

RSLC Column6 Acceleration

Double Velocity12 Acceleration

mAU

mAU

5

Separation of Pigments in TurmericTurmericcontainsthreemajor

yellowpigmentsknownascurcumi-noids,asshowninFigure8.TheuniqueselectivityoftheAcclaimPA2columngivesresolutionfarsuperiortoC18.Threecomponentsarewell-resolvedonanAcclaimPA2column,whiletotalco-elutionisobservedonaC18columnevenwhenpackedwithsmallsilicaparticles,asshowninFigure9.

Figure 8. Structures of turmeric pigments

Figure 9. Separation of pigments in turmeric using Acclaim RSLC C18 and RSLC PA2 columns. Selectivity is more important than efficiency.

R R'

OH O

OHHO Compound R R'Curcumin OCH3 OCH3Demethoxycurcumin OCH3 HBis-demethoxycurcumin H H

25401

Peak

123

0 1 2 3 4

0

200

mAU

Minutes

C18

PA2

1

2 3

1, 2, 3

Area: 9.7 mAU*Min.

Total area: 9.9 mAU*Min.

Columns: Acclaim RSLC 120 C18 Acclaim RSLC PA2Dimensions: 2.2 m, 2.1 100 mmMobile Phase A: 15 mM H3PO4Mobile Phase B: MethanolIsocratic: C18: 70% B (v/v) PA2: 80% B (v/v)Flow: 0.41 mL/minTemperature: 30 CDetector: UV, 428 nmSample: Turmeric extractPeaks: 1. Curcumin 2. Demethoxycurcumin 3. Bis-demethoxycurcumin

25402

6

Figure 10. Accelerated separation of pigments in turmeric using A) Acclaim PA2 5 m, B) Acclaim RSLC PA2 3 m, and C) Acclaim RSLC PA2 2.2 m columns.

Figure 11. Ultrafast separation of pigments in turmeric using the Acclaim RSLC PA2 2.2 m column. Increased flow, temperature, and pressure further accelerate the method.

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2

0

200

mAU

Minutes

1

2 3

The original method took 12 min. The fast method requires only 0.60 min, a 20-fold increase in speed of analysis.

Column: Acclaim RSLC, PA2, 2.2 m 2.1 50 mmMobile Phases: (A) 15 mM H3PO4 in D.I. water (B) methanolDetector: UV, 428 nmSample: Turmeric extractPeaks: 1. Curcumin 2. Demethoxycurcumin 3. Bis-demethoxycurcumin

Boost Flow rate C %CH3OH Pressure (bar) R1,2 N11.00 0.41 30 80 181 4.0 27002.45 1.00 30 80 435 2.8 15003.66 1.50 50 75 509 2.4 1700

25404

Method Speed-UpTheseparationofthreecurcumi-

noidscanbeachievedonanAcclaimPA2,5m,4.6150mmcolumnin10min,asshowninFigure10.UsingtheMethodSpeed-UpCalculatortodetermineappropriateflowratesandinjectionvolumes,thisseparationcanbetransferredtoAcclaimRSLCcol-umnswithsubstantialtimesavingsandsubstantialreductioninmobilephaseconsumption.Inthisexample,anAcclaimRSLCPA2,3m,3.075mmcolumnprovidesthreefoldaccelerationand80%mobilephasesav-ings,whileanAcclaimRSLCPA2,2.2m,2.150mmcolumngivesfivefoldspeedupwith92%mobilephasereduc-tion.

Furtherthroughputincreasecanbeachievedbyraisingtheflowrateandcolumntemperature,asshowninFigure11.TheMethodSpeed-UpCalculatorpredictsbackpressureincreasesresultingfromchangesinflowrate,suggestingthatevenfastermethodsarefeasiblewiththeRSLCcolumns.Astheresult,a12minmethodiseasilytransformedintoa0.6minRSLCmethod(a20-foldacceleration)whileconsumingonly8%oforiginalmobilephasevolume.

0 1 2 3 4 5 6 7 8 9 10 11 12

0

450

mAU

Minutes

1

3

C. Acclaim RSLC, PA2, 2.2 m, 2.1 50 mm

B. Acclaim RSLC, PA2, 3 m, 3.0 75 mm

A. Acclaim PA2, 5 m, 4.6 150 mm

Column: Acclaim PolarAdvantage II (PA2)Mobile Phases: (A) 15 mM H3PO4 in D.I. water (B) methanolIsocratic: 80% BFlow: See table.Temperature: 30 CDetector: UV, 428 nmSample: Turmeric extractPeaks: 1. Curcumin 2. Demethoxycurcumin 3. Bis-demethoxycurcumin

Geometry Flow Rate Pressure (bar) R1,2 N1 Speed Solvent UseA. 5 m, 4.6 150 mm 1.00 78 4.0 4400 1 100%B. 3 m, 3.0 75 mm 0.61 145 3.9 3800 3 21%C. 2.2 m, 2.1 50 mm 0.41 188 4.0 2700 5 8%

2

25403

7

Figure 13. USP assay of aspirin on the A) Acclaim 120 C18 5 m and B) Acclaim RSLC 120 C18 2.2 m columns.

Figure 12. Separation of artichoke antioxidants using A) Acclaim PA2 5 m and B) Acclaim RSLC PA2 2.2 m columns.

Gradient A: Flow 1.00 mL/min, Backpressure 1200 psi Time -7 0 2.5 16 18 min %A 15 15 15 40 40 %B 85 85 85 60 60

Gradient B: Flow 0.41 mL/min, Backpressure 2300 psi Time -5.4 0 4.8 6.1 min %A 15 15 40 40 %B 85 85 60 60 Bypass injector valve at 0.20 min

Column: Acclaim PA2 Dimensions: A: 5 m, 4.6 150 mm B: 2.2 m, 2.1 100 mmLC System: UltiMate 3000 RSLCMobile Phases: (A) Acetonitrile (B) 15 mM H3PO4Inj. Volume: A: 5 L B: 0.8 LTemperature: 30 CDetection: UV, 330 nmSample: Extract of artichoke stem in 60% CH3OH

Peaks: 1. 3-Caffeoylquinic acid 2. 1,5-Dicaffeoylquinic acid *Unidentified caffeic acid derivative

25536Minutes

0 5 10 15 18 0

350

mAU

A

0.0 2.0 4.0 6.1 0

350

mAU

B

1

2

*

R = 1.43

R = 1.44

1 2

*

Column: Acclaim 120 C18 A: 5 m, 4.6 250 mm B: 2.2 m, 2.1 50 mmMobile Phase: 15% (v/v) Acetonitrile + 0.2% Na heptanesulfonate adj.to pH 3.4 with HOAcFlow Rate: A: 2.00 mL/min B: 0.81 mL/minTemperature: 25 CInj. Volume: A: 10 L B: 1.0 L

Detection: DAD-3000 RS, UV, 280 nm Peaks: 1. Salicylic acid 15 g/mL 2. Aspirin 500 In 99:1 acetonitrile:formic acidReference: USP -29 NF-24 page 200

1

1

2

2

B

A

R1,2 = 2.9

R1,2 = 5.7

0 4 8 12 16

0

50

mAU

Minutes 25537

Antioxidant Separation in AritchokeArtichoke(Cynara scolymus L.)is

reputedtohaveavarietyofbeneficialbiologicaleffects.Mono-anddicaf-feoylquinicacidderivativesarebelievedtobetheactivesubstances.TheAcclaimPolarAdvantageIIcolumnprovidessuperiorselectivityandresolutionforthisgroupofcompoundscomparedtoconventionalC18columns;theun-knownandpeak2co-eluteonC18.TheRSLCcolumnisthreetimesfasterandusesonly16%ofthesolvent,yetproducesthesamequalityofchromato-gram(Figure12).

Accelerated USP Assay of AspirinTheoriginalUSPmethodusesa

4300mmcolumnataflowrateof2mL/min.Itdeliversresolutionof5.7whenusedwiththeAcclaim120C18column.ThisisanexcellentcandidateforaccelerationwithRSLC(Figure13).Simplyre-computingtheoperatingparametersfora2.150mmcolumn,usingtheDionexMethodSpeed-UpCalculator,leadstoa10-foldincreaseinthroughputand96%savingsinmobilephaseperassay.Theacceleratedmethodstillgivesbaselineresolutionofthetwocomponents.

8

Chlorinated Acids in Water Chlorinated acids are used as herbi-

cides, and sometimes are found to con-taminate water supplies. EPA Method 555 is an HPLC method for the determination of certain chlorinated acids in ground water and finished drinking water using reversed-phase columns and diode-array detection. Figure 14 shows a fast, high-resolution separation is achieved using the Acclaim RSLC PolarAdvantage 2.2 m, 2.1 100 mm column, with an analysis cycle of about 12 min.

Pyrethrin in SeedsPyrethrin is a natural insecticide

extracted from seeds of Chrysanthemum cinerariaefolium. Pyrethroids are synthetic analogs. These are nonpersistent, have relatively low mammalian toxicity, and have replaced organophosphate insecticides in many applications. The synthetic substances are produced as a mixture of isomers, hence the complex chromatograms shown in Figure 15. The Acclaim RSLC 120 C18 2.2 m column in the 2.1 250 mm format offers extraordinary efficiency (>40,000 theoretical plates/column) and pressure rating (1200 bar) for the ultimate in high-resolution LC.

Phenols in WastewaterPhenolsareoftenfoundinmunici-

palandindustrialwastewater.Theyaretraditionallyanalyzedbygaschromato-graphic(GC)methodssuchasU.S.EPAMethod604andMethod625,butHPLCcanbeusedtoseparateanddetectavarietyoftheseimportantcompounds.Figure16showsthefast,high-resolutioncolumnAcclaimRSLCPolarAdvantage(PA)2.2m3.050mmcolumn,theanalysiscycleiscompleteinunder5min.

27990

0 2 4 6 8

0

250

mAU

Minutes

Mix A

Mix B

1 2

3

4 5 6 7

8

9

10 11 12 13

14 15

16

Chloramben

200 400 nm

213.7

315.6

Gradient Time (min): -4.0 0.0 0.2 4.0 4.0 8.0%A 80 80 80 35 10 10%B 20 20 20 65 90 90Flow Rate: 0.50 mL/minInjection Volume: 2.0 LTemperature: 40 CDetection: UV at 230 nm, 5 Hz, 0.4 s resp. time Spectra 200400 nmSamples: Calibration mixes A & B, 25 g/mL in waterPeaks: Mix A Mix B 1. Picloram 9. 4-Nitrophenol 2. Chloramben 10. MCPA 3. Dicamba 11. 3,5-Dichlorobenzoic 4. Bentazon 12. MCPP 5. 2,4-D 13. 2,4,5-T 6. Dichlorprop 14. 2,4-DB 7. 2,4,5-TP 15. Dinoseb 8. Acifluorfen 16. Pentachlorophenol

Column: Acclaim RSLC PolarAdvantage, 2.2 mDimensions: 2.1 100 mmHPLC System: UltiMate 3000 RSLC QuaternaryMobile Phases: A: 5 mM Phosphoric acid B: Acetonitrile

Figure 14. Chlorinated Acids Separation (EPA Method 555).

27973

Flow Rate: 0.35 mL/minInjection Volume: 0.5 LTemperature: 45 CDetection: Diode array, UV at 220 nmPeaks: 1. Pyrethrin II 100 g/mL 2. cis & trans Tetramethrin 3. cis & trans Allethrin 4. Pyrethrin I 5. Baythroid 6. Fenvalerate 7. cis & trans Resmethrin 8. cis & trans Permethrin 9. cis & trans Phenothrin

Column: Acclaim RSLC 120 C18, 2.2 mDimensions: 2.1 250 mmLC System: UltiMate 3000 RSLC Mobile Phases: Methanol:water 80:20 (v/v)

1

2

3

4

5 6

7

8

9

0 5 10 15 20 28

0

70

mAU

Minutes

(A)

(B)

Figure 15. Ultrahigh efficiency separation of insecticides.

27652Minutes

mAU

0

60

1

2 3

4

5

6

7

8 9 10

11

0 0.5 1.0 1.5 2.0 2.5 3.0

Gradient Time (min): -1.5 0.0 0.3 2.6 3.0 %A 70 70 70 10 10 %B 30 30 30 90 90 Flow Rate: 1.25 mL/minInjection Volume: 0.5 LTemperature: 30 CDetection: UV at 280 nm, 10 Hz, 0.5 s resp. timeSamples: Calibration mixes, 50 g/mL in waterPeaks: 1. Phenol 2. 2,4-Dinitrophenol 3. 4-Nitrophenol 4. 2-Chlorophenol 5. 2-Nitrophenol 6. 2,4-Dimethylphenol 7. 4,6-Dinitro-2-methylphenol 8. 4-Chloro-3-methylphenol 9. 2,4-Dichlorophenol 10. 2,4,6-Trichlorophenol 11. Pentachlorophenol

Column: Acclaim RSLC PolarAdvantage, 2.2 mDimensions: 3.0 50 mmSystem: UltiMate 3000 RSLC Mobile Phases: A: 10 mM formic acid + 10 mM ammonium formate, pH 3.75 0.05 B: Acetonitrile

Figure 16. Fast, high-resolution phenol separation (EPA 604).

9

specificationsColumn Acclaim120C8 Acclaim120C18 AcclaimPA AcclaimPA2BondedPhase C8 C18 Sulfonamide-embedded Amide-embeddedEndcapped Yes Yes Yes Yes

Substrate 2.2multrapuresphericalsilica2.2multrapuresphericalsilica

2.2multrapuresphericalsilica

2.2multrapuresphericalsilica

AveragePoreDiameter 120 120 120 120SurfaceArea(m2/g) 320 320 320 320TotalCarbonContent* 10% 17% 16% 16%pHRange 2.08.0 2.08.0 2.08.0 1.510MetalImpurity(ppm)Na,Fe,Al

ordering information

ToorderintheU.S.,call(800)346-6390orcontacttheDionexRegionalOfficenearestyou.OutsidetheU.S.,orderthroughyourlocalDionexofficeordistributor.Refertothefollowingpartnumbers.

Acclaim RSLC Columns

Acclaim RSLC 120 C18 Columns AcclaimRSLC120,C18,2.2m,2.130mm.........................................071400 AcclaimRSLC120C18,2.2m,2.150mm..........................................068981 AcclaimRSLC120C18,2.2m,2.1100mm........................................068982 AcclaimRSLC120C18,2.2m,2.1150mm........................................071399 AcclaimRSLC120,C18,2.2m,2.1250mm.......................................074812 AcclaimRSLC120,C18,2.2m,3.030mm.........................................071606 AcclaimRSLC120C18,2.2m,3.050mm..........................................071605 AcclaimRSLC120C18,2.2m,3.075mm..........................................075697 AcclaimRSLC120C18,2.2m,3.0100mm........................................071604 Acclaim RSLC 120 C8 Columns AcclaimRSLC120,C8,2.2m,2.130mm...........................................072614 AcclaimRSLC120C8,2.2m,2.150mm............................................072615 AcclaimRSLC120C8,2.2m,2.1100mm..........................................072616 AcclaimRSLC120C8,2.2m,2.1150mm..........................................072617 AcclaimRSLC120,C8,2.2m,2.1250mm......................................... 074811 AcclaimRSLC120,C8,2.2m,3.030mm...........................................072618 AcclaimRSLC120C8,2.2m,3.050mm............................................072619 AcclaimRSLC120C8,2.2m,3.075mm............................................075696 AcclaimRSLC120C8,2.2m,3.0100mm..........................................072620 Acclaim RSLC PolarAdvantage Columns AcclaimRSLCPolarAdvantage(PA),2.2m,2.130mm.....................072621 AcclaimRSLCPolarAdvantage(PA),2.2m,2.150mm.....................072622 AcclaimRSLCPolarAdvantage(PA),2.2m,2.1100mm...................072623 AcclaimRSLCPolarAdvantage(PA),2.2m,2.1150mm...................072624 AcclaimRSLCPolarAdvantage(PA),2.2m,2.1250mm..................074813 AcclaimRSLCPolarAdvantage(PA),2.2m,3.030mm.....................072625 AcclaimRSLCPolarAdvantage(PA),2.2m,3.050mm.....................072626 AcclaimRSLCPolarAdvantage(PA),2.2m,3.075mm....................075698 AcclaimRSLCPolarAdvantage(PA),2.2m,3.0100mm...................072627 Acclaim RSLC PolarAdvantage II Columns AcclaimRSLCPolarAdvantageII(PA2),2.2m,2.130mm................071402 AcclaimRSLCPolarAdvantageII(PA2),2.2m,2.150mm................068989 AcclaimRSLCPolarAdvantageII(PA2),2.2m,2.1100mm..............068990 AcclaimRSLCPolarAdvantageII(PA2),2.2m,2.1150mm..............071401 AcclaimRSLCPolarAdvantageII(PA2),2.2m,2.1250mm.............074814 AcclaimRSLCPolarAdvantageII(PA2),2.2m,3.030mm................071609 AcclaimRSLCPolarAdvantageII(PA2),2.2m,3.050mm................071608 AcclaimRSLCPolarAdvantageII(PA2),2.2m,3.075mm...............075699 AcclaimRSLCPolarAdvantageII(PA2),2.2m,3.0100mm..............071607

Dionex Method Speed-Up Calculator

AvailablefreeofchargefromyourDionexrepresentative.AcclaimandUltiMateareregisteredtrademarksofDionexCorporation.

Passion. Power. Productivity.

LPN 2122-02 PDF 2/11 2011 Dionex Corporation

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