Download - TP CAPSICUM ANNUUM 076-2

CPVO-TP/076/2 Final English

Date: 21/03/2007

PROTOCOL FOR DISTINCTNESS, UNIFORMITY AND STABILITY TESTS

Capsicum annuum L.

SWEET PEPPER, HOT PEPPER, PAPRIKA, CHILI

UPOV Species Code: CAPSI_ANN

Adopted on 21/03/2007

European Union Community Plant Variety Office


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I SUBJECT OF THE PROTOCOL

The protocol describes the technical procedures to be followed in order to meet the Council Regulation (EC) No. 2100/94 on Community Plant Variety Rights. The technical procedures have been agreed by the Administrative Council and are based on general UPOV Document TG/1/3 and UPOV Guideline TG/76/8 dated 05/04/2006 for the conduct of tests for Distinctness, Uniformity and Stability. This protocol applies for all varieties of Capsicum annuum L.

II SUBMISSION OF SEED AND OTHER PLANT MATERIAL

1. The Community Plant Variety Office (CPVO) is responsible for informing the applicant of

• the closing date for the receipt of plant material; • the minimum amount and quality of plant material required; • the Examination Office to which material is to be sent.

A sub-sample of the material submitted for test will be held in the variety collection as the definitive sample of the candidate variety. The applicant is responsible for ensuring compliance with any customs and plant health requirements.

2. Final dates for receipt of documentation and material by the Examination Office The final dates for receipt of requests, technical questionnaires and the final date or submission period for plant material will be decided by the CPVO and each Examination Office chosen. The Examination Office is responsible for immediately acknowledging the receipt of requests for testing, and technical questionnaires. Immediately after the closing date for the receipt of plant material the Examination Office should inform the CPVO whether acceptable plant material has been received or not. However if unsatisfactory plant material is submitted the CPVO should be informed as soon as possible.

3. Plant material requirements The current quality and quantity requirements as well as the final dates for submission of the plant material are available on the CPVO website (www.cpvo.europa.eu) and are published in the CPVO gazette ‘S2’.


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Quality of seeds: Should not be less than the standards laid down for certified seed in Annex II of Council Directive 2002/55/EC.

Seed treatment: The plant material must not have undergone any treatment unless the CPVO and the Examination Office allow or request such treatment. If it has been treated, full details of the treatment must be given.

Special requirement: - Labelling of sample: - Species - File number of the application allocated by the CPVO

- Breeder's reference - Examination office’s reference (if known) - Name of applicant - The phrase “On request of the CPVO”

III CONDUCT OF TESTS

1. Variety collection

A variety collection will be maintained for the purpose of establishing distinctness of the candidate varieties in test. A variety collection may contain both living material and descriptive information. A variety will be included in a variety collection only if plant material is available to make a technical examination. Pursuant to Article 7 of Council Regulation (EC) No. 2100/94, the basis for a collection should be the following: • varieties listed or protected at the EU level or at least in one of the EEA Member

States; • varieties protected in other UPOV Member States; • any other variety in common knowledge. The composition of the variety collection in each Examination Office depends on the environmental conditions in which the Examination Office is located. Variety collections will be held under conditions which ensure the long term maintenance of each accession. It is the responsibility of Examination Offices to replace reference material which has deteriorated or become depleted. Replacement material can only be introduced if appropriate tests confirm conformity with the existing reference material. If any difficulties arise for the replacement of reference material, Examination Offices must inform the CPVO. If authentic plant material of a variety cannot be supplied to an Examination Office the variety will be removed from the variety collection.


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2. Material to be examined Candidate varieties will be directly compared with other candidates for Community plant variety rights tested at the same Examination Office, and with appropriate varieties in the variety collection. When necessary an Examination Office may also include other candidates and varieties. Examination Offices should therefore make efforts to co-ordinate the work with other Offices involved in DUS testing of pepper. There should be at least an exchange of technical questionnaires for each candidate variety, and during the test period, Examination Offices should notify each other and the CPVO of candidate varieties which are likely to present problems in establishing distinctness. In order to solve particular problems Examination Offices may exchange plant material.

3. Characteristics to be used The characteristics to be used in DUS tests and preparation of descriptions shall be those referred to in the Annex I. All the characteristics shall be used, providing that observation of a characteristic is not rendered impossible by the expression of any other characteristic, or the expression of a characteristic is prevented by the environmental conditions under which the test is conducted. In the latter case, the CPVO should be informed. In addition the existence of some other regulation e.g. plant health, may make the observation of the characteristic impossible. The Administrative Council empowers the President, in accordance with Article 23 of Commission Regulation (EC) No. 1239/95, to insert additional characteristics and their expression in respect of a variety.

4. Grouping of varieties The varieties and candidates to be compared will be divided into groups to facilitate the assessment of distinctness. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly, within a variety and which in their various states of expression are fairly evenly distributed throughout the collection. In the case of continuous grouping characteristics overlapping states of expression between adjacent groups is required to reduce the risks of incorrect allocation of candidates to groups. The characteristics used for grouping could be the following: a) Seedling: anthocyanin coloration of hypocotyl (characteristic 1)

b) Plant: shortened internode (in upper part) (characteristic 3)

c) Fruit: colour (before maturity) (characteristic 20)

d) Fruit: shape in longitudinal section (characteristic 27)

e) Fruit: colour (at maturity) (characteristic 32)

f) Fruit: number of locules (characteristic 39)

g) Fruit: capsaicin in placenta (characteristic 44)


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h) Resistance to Tobamovirus, Pathotype P0 (Tobacco Mosaic Virus (0)) (characteristic 47.1)

i) Resistance to Tobamovirus, Pathotype P1-2 (Pepper Mild Mottle Virus (1-2)) (characteristic 47.3)

j) Resistance to Tobamovirus, Pathotype P1-2-3 (Pepper Mild Mottle Virus (1-2-3)) (characteristic 47.4)

k) Resistance to Potato Virus Y (PVY) Pathotype P0 (characteristic 48.1)

5. Trial designs and growing conditions The minimum duration of tests will normally be two independent growing cycles. For vegetatively propagated varieties, the duration of the testing may be reduced to one growing cycle if the results on distinctness and uniformity are conclusive. Tests will be carried out under conditions ensuring normal growth. The size of the plots will be such that plants or parts of plants may be removed for measuring and counting without prejudice to the observations which must be made up to the end of the growing period. The test design is as follows As a minimum, each test should include a total of 20 plants, which should be divided between two or more replicates. All observations determined by measurement or counting should be made on 20 plants or parts of 20 plants.

6. Special tests In accordance with Article 83(3) of Council Regulation (EC) No. 2100/94 an applicant may claim either in the Technical Questionnaire or during the test that a candidate variety has a characteristic which would be helpful in establishing distinctness. If such a claim is made and is supported by reliable technical data, a special test may be undertaken providing that a technically acceptable test procedure can be devised. Special tests will be undertaken, with the agreement of the President of CPVO, where distinctness is unlikely to be shown using the characters listed in the protocol.

7. Standards for decisions a) Distinctness A candidate variety will be considered to be distinct if it meets the requirements of Article 7 of Council Regulation (EC) No. 2100/94.


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b) Uniformity A hybrid variety will be considered to be sufficiently uniform if the number of off-types does not exceed the number of plants as indicated in the table below. A population standard of 1% and an acceptance probability of 95% should be applied. Table of maximum numbers of off-types allowed for uniformity standards.

Number of plants off-types allowed

6-35 1 An open-pollinated variety will be considered to be sufficiently uniform if the number of off-types does not exceed the number of plants as indicated in the table below. A population standard of 2% and an acceptance probability of 95% should be applied. Table of maximum numbers of off-types allowed for uniformity standards.

Number of plants off-types allowed

19-41 2 c) Stability A candidate will be considered to be sufficiently stable when there is no evidence to indicate that it lacks uniformity.

IV REPORTING OF RESULTS

After each recording season the results will be summarised and reported to the CPVO in the form of a UPOV model interim report in which any problems will be indicated under the headings distinctness, uniformity and stability. Candidates may meet the DUS standards after two growing periods but in some cases three growing periods may be required. When tests are completed the results will be sent by the Examination Office to the CPVO in the form of a UPOV model final report.

If it is considered that the candidate complies with the DUS standards, the final report will be accompanied by a variety description in the format recommended by UPOV. If not the reasons for failure and a summary of the test results will be included with the final report.

The CPVO must receive from the Examination Office interim reports and final reports by the date agreed between the CPVO and the examination office.

Interim reports and final examination reports shall be signed by the responsible member of the staff of the Examination Office and shall expressly acknowledge the exclusive rights of disposal of CPVO.


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V LIAISON WITH THE APPLICANT

If problems arise during the course of the test the CPVO should be informed immediately so that the information can be passed on to the applicant. Subject to prior agreement, the applicant may be directly informed at the same time as the CPVO particularly if a visit to the trial is advisable. The interim report as well as the final report shall be sent by the Examination Office to the CPVO.

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ANNEXES TO FOLLOW

ANNEX I PAGE

Table of characteristics ................................................................................................. 10 Explanations and methods ............................................................................................ 22 Legend:

Note: For the CPVO numbered characteristics, all characteristics in the table are compulsory; notwithstanding, in the case of disease resistance characteristics, only those resistances marked with an asterisk (*) in the CPVO column are compulsory. The asterisks in the UPOV numbered characteristics are there for information purposes and denote those characteristics which should always be observed when a UPOV guideline is utilised. Test with P1-2-3: For varieties claimed and testing resistance “present” (9) to Tobamovirus pathotype P1-2-3, it is not necessary to test pathotypes P0 and P1-2 anymore (because of genetics result can only be resistant, hencewith marked [*]). If however tested susceptible ‘’absent” (1) to pathotype P1-2-3 it compulsory to test for P1-2. Test with P1-2: For varieties tested susceptible ‘’absent” (1) to pathotype P1-2-3 or claimed resistant “present” to pathotype P1-2 and testing resistance “present” (9) to Tobamovirus pathotype P1-2, it is not necessary to test pathotypes P0 anymore (because of genetics result can only be resistant, hencewith marked [*]). If however tested susceptible ‘’absent” (1) to pathotype P1-2 it compulsory to test for P0. Note that if claimed and tested resistant to pathotype P1-2 it is also compulsory to check P1-2-3. Test with P1 (not compulsory): For varieties tested susceptible ‘’absent” (1) to pathotype P1-2 or claimed resistant “present” to pathotype P1 and testing resistance “present” (9) to Tobamovirus pathotype P1, it is not necessary to test pathotypes P0 anymore (because of genetics result can only be resistant, hencewith marked [*]). If however tested susceptible ‘’absent” (1) to pathotype P1 it compulsory to test for P0. Note that if claimed and tested resistant to pathotype P1 it is also compulsory to check P1-2 and eventually even P1-2-3. Test with P0: For varieties claimed and testing resistance “absent” (1) to Tobamovirus pathotype P0, it is only necessary to test pathotypes P0 (because of genetics result can only be susceptible for P1, P1-2 and P1-2-3). If however claimed resistance “absent” but tested resistant ‘’present” (9) to pathotype P0 it compulsory to test for P1-2. Note that for varieties claimed and testing resistance “present” (9) to Tobamovirus pathotype P0, it is compulsory to check pathotype P1-2 and eventually even P1-2-3.


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In general for the assessment of resistance characteristics, the facilities of other Examination Offices or specialised institutions might be used, subject to previous arrangements. Some characteristics may be discarded: if there are already phytosanitary restrictions. (+) See explanations on the Table of characteristics (a) – (b) See explanations on the table of characteristics Types of expression of characteristics: QL – Qualitative characteristic QN – Quantitative characteristic PQ – Pseudo-qualitative characteristic Type of observation of characteristics: MG – Single measurement of a group of plants or parts of plants MS – Measurement of a number of individual plants or parts of plants VG – Visual assessment by a single observation of a group of plants or parts of plants VS – Visual assessment by observation of individual plants or parts of plants When a method of observation is attributed to a certain characteristic, the first differentiation is made depending if the action taken is a visual observation (V) or a measurement (M). The second differentiation deals with the number of observations the expert attributes to each variety, thus the attribution of either G or S. If a single observation of a group consisting of an undefined number of individual plants is appropriate to assess the expression of a variety, we talk about a visual observation or a measurement made on a group of plants, thus we attribute the letter G (either VG or MG). If the expert makes more than one observation on that group of plants, the decisive part is that we have at the end only one data entry per variety which means that we have to deal with G (e.g. measurement of plant length on a plot – MG, visual observation of green colour of leaves on a plot – VG). If it is necessary to observe a number of individual plants to assess the expression of a variety, we should attribute the letter S (thus either VS or MS). Single plant data entries are kept per variety for further calculations like the variety mean (e.g. measurement of length of ears – MS, visual observation of growth habit of single plants in grasses – VS). The number of individual plants to be observed in such cases is stated in section III.5.

Literature ...................................................................................................................... 35

ANNEX II

Technical Questionnaire


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ANNEX I

TABLE OF CHARACTERISTICS TO BE USED IN DUS TESTS AND PREPARATION OF DESCRIPTIONS

CPVO N°

UPOV N°

Stage, Method

Characteristics Examples Note

1. 1. QL Seedling: anthocyanin coloration of hypocotyl

(*) VG absent Albaregia, Albena 1

G present Lamuyo 9

2. 3. QN Plant: length of stem

(+) (+) MS/VG short Delphin, Trophy 3

medium Belsir, Lamuyo 5

long Lipari, Marconi, Rouge long ordinaire

7

3. (+)

4. (+)

QL Plant: shortened internode (in upper part)

(*) VG absent California wonder, De Cayenne 1

G present Fehér, Kalocsai 601, Kalocsai 702 9

4. (+)

5. (+)

QN Varieties with shortened internodes only: Plant: number of internodes between the first flower and shortened internodes

MS none Kalocsai 601 1

one to three Fehér 2

more than three Kalocsai 702 3

5. 6. QN Varieties without shortened internodes only: Plant: length of internode (on primary side shoots)

MS/ VG very short Albaregia 1

short Bandero, Blondy, Danubia, Tenor 3

medium Dolmi, Florian, Órias 5

long Corno di toro rosso 7

very long Fenice, Kalocsai M, Sienor 9


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CPVO N°

UPOV N°

Stage, Method


6. 7. QL Plant: anthocyanin coloration of nodes

VG absent Albaregia 1

present California wonder 9

7. 8. QN Stem: intensity of anthocyanin coloration of nodes

VG very weak 1

weak California wonder, Clio, Doux d’Espagne, Doux très long des Landes, Golden calwonder

3

medium Clovis, Lamuyo, Sonar 5

strong Piquant d’Algérie, Zarai 7

very strong Alwin, Koral, Lito, Pusztagold 9

8. 9. QN Stem: hairiness of nodes

VG absent or very weak Arlequin 1

weak Andevalo, Clovis 3

medium Doux très long des Landes, Farnese 5

strong Fenice, Solario 7

very strong Mino 9

9. 10. QN Plant: height

(+) (+) VG/MS very short Kalocsai 601 1

short Albaregia 3

medium HRF 5

tall Century, Orias 7

very tall Hot chili 9


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CPVO N°

UPOV N°

Stage, Method


10. 11. QN Leaf: length of blade

MS/VG very short Macska sárga, Tüzes piros 1

short De Cayenne, Szentesi cseresznye 3

medium Atol, Blondy, Marconi, Merit, Anthea

5

long Cupido, Dolmy, Encore, Mazurka, Monte

7

very long Predi, Solario 9

11. 12. QN Leaf: width of blade

MS/VG very narrow Macska sárga, Recio, Tüzes piros 1

narrow De Cayenne, Pusztagold, Szentesi cseresznye

3

medium Albaregia, Balaton, Danubia, Marconi, Merit

5

broad California Wonder, Golden calwonder, Sienor, Solario

7

12. 13. QN Leaf: intensity of green colour

VG very light Amaryllis, Lombardo 1

light Piquant d’Algérie, Pusztagold 3

medium Doux très long des Landes, Merit 5

dark Dolmy,Tinto 7

very dark Hot Chilli, Recio, Soleor 9

13. 14. PQ Leaf: shape

(+) (+) VG lanceolate Diavolo, Recio 1

ovate Balico, Sonar 2

broad elliptic Solario 3


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CPVO N°

UPOV N°

Stage, Method


14. 15. QN Leaf: undulation of margin

VG absent or very weak De Cayenne 1

weak Doux très long des Landes 3

medium Tenor 5

strong Sucette de Provence, Tosca 7

very strong Farya 9

15. 16. QN Leaf: blistering

VG very weak Century, Recio, Sofiane 1

weak Pusztagold 3

medium Merit 5

strong Greygo, PAZ pallagi 7

very strong Florian 9

16. 17. QN Leaf: profile in cross section

(+) (+) VG strongly concave Slávy 1

moderately concave Doux italien, Favolor 3

flat De Cayenne, Recio 5

moderately convex Albaregia 7

strongly convex Tinto 9

17. 18. QN Leaf: glossiness

VG very weak Diavolo 1

weak De Cayenne, Doux très long des Landes

3

medium Alby, Eolo 5

strong Andevalo, Floridor 7

very strong Cubor, Petit Marseillais 9


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CPVO N°

UPOV N°

Stage, Method


18. 19. QN Peduncle: attitude

(+) (+) VG erect Fehér, Red Chili 1

(*) semi-drooping Blondy 2

drooping Heldor, Lamuyo 3

19. 20. QL Flower: anthocyanin coloration in anther

VG absent Danza 1

present Lamuyo 9

20. 21. PQ Fruit: colour (before maturity)

(a) (*) VG greenish white Blanc d’Espagne 1

(a) yellow Fehér, Sweet banana 2

green California wonder, Lamuyo 3

G purple Nigra 4

21. 22. QN Fruit: intensity of colour (before maturity)

(a) (a) VG very light 1

light 3

medium 5

dark 7

very dark 9

22. 23. QL Fruit: anthocyanin coloration (before maturity)

(a) (a) VG absent Lamuyo 1

present Alabástrom, Purple beauty 9

23. 24. QN Fruit: attitude

(b) (b) VG erect Kalocsai 601, Red Chili 1

horizontal PAZ szentesi, Vinedale 2

drooping De Cayenne, Lamuyo 3


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CPVO N°

UPOV N°

Stage, Method


24. 25. QN Fruit: length

(b) (b) VG/MS very short Cherry Sweet, Topgirl 1

short Delphin, Petit carré doux 3

medium Fehér, Lamuyo 5

long Doux d’Espagne, Majister 7

very long Arabal, Corno di toro, Marconi 9

25. 26. QN Fruit: diameter

(b) (b) VG/MS very narrow De Cayenne, Recio 1

narrow Doux très long des Landes 3

medium Doux italien, Corno di toro 5

broad Clovis, Lamuyo 7

very broad Floridor, Ibleor, Inca, Joly rosso, Quadrato d’Asti, Surpas

9

26. 27. QN Fruit: ratio length/diameter

(b) (*) MS very small Liebesapfel, PAZ szentesi, Rotopa 1

(b) small Bucano, Topgirl 3

medium Adra, Cherry Sweet, Daniel, Delphin, Edino

5

large Heldor, Lamuyo, Magister, Tenno, Vidi

7

very large De Cayenne, Kusamon, Spadi 9


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CPVO N°

UPOV N°

Stage, Method


27. 28. PQ Fruit: shape in longitudinal section

(+) (+) VG oblate Liebesapfel, PAZ szentesi, Topepo rosso

1

(*) circular Cherry Sweet 2

(b) (b) cordate Daniel 3

square Delphin, Yolo Wonder 4

rectangular Clovis, Nocera rosso 5

trapezoidal Delta, Piperade 6

moderately triangular Fehér, Marconi 7

narrowly triangular De Cayenne, Demon 8

G horn shaped Tauro 9

28. 29. PQ Fruit: shape in cross section (at level of placenta)

(b) (b) VG elliptic Sweet banana 1

angular Vinedale 2

circular Cherry Sweet, Doux très long des Landes

3

29. 30. QN Fruit: sinuation of pericarp at basal part

(+) (+) VG absent or very weak Delphin, Kalocsai V-2, Milord 1

(b) (b) weak Donat 3

medium Duna, Banán 5

strong Alfa 7

very strong Édes spiral, Doux italien 9


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CPVO N°

UPOV N°

Stage, Method


30. 31. QN Fruit: sinuation of pericarp excluding basal part

(+) (+) VG absent or very weak Delphin, Milord 1

(b) (b) weak Clovis, Sonar 3

medium Ursus 5

strong De Cayenne, Doux italien 7

very strong Arabal 9

31. 32. QN Fruit: texture of surface

(b) (*) VG smooth or very slightly wrinkled Milord 1

(b) slightly wrinkled Doux très long des Landes 2

strongly wrinkled Sierra Nevada 3

32. 33. PQ Fruit: colour (at maturity)

(b) (*) VG yellow Golden calwonder, Heldor 1

(b) orange Ariane 2

red Fehér, Lamuyo 3

brown Brupa, Negral 4

G green Green6203 5

33. 34. QN Fruit: intensity of colour (at maturity)

(b) (b) VG light 3

medium 5

dark 7

34. 35. QN Fruit: glossiness

(b) (b) VG very weak Macska sárga, Pikanta 1

weak Doux très long des Landes 3

medium Carré doux extra hâtif, Lamuyo, Sonar

5

strong Doux italien, Trophy 7

very strong Floridor, Kappy 9


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CPVO N°

UPOV N°

Stage, Method


35. 36. QL Fruit: stalk cavity

(b) (*) VG absent Corinto, Corno di toro, Sweet banana, Sucette de Provence

1

(b) present Bingor, Lamuyo 9

36. 37. QN Fruit: depth of stalk cavity

(b) (b) VG very shallow Flush, Kaméleon, Niagara 1

shallow Delphin, Doux italien, Fehér, Latino 3

medium Lamuyo, Magister 5

deep Osir, Quadrato d’Asti rosso, Surpas 7

very deep Cancun, Cubor, Pablor, Shy Beauty 9

37. 38. PQ Fruit: shape of apex

(b) (b) VG very acute De Cayenne, Hot Chilli 1

moderately acute 2

rounded Cherry Sweet 3

moderately depressed Quadrato d’Asti rosso 4

very depressed Kerala, Monte, Osir 5

38. 39. QN Fruit: depth of interloculary grooves

(+) (+) VG absent or very shallow De Cayenne 1

(b) (b) shallow Milord, Topgirl 3

medium Clovis, Lamuyo, Marconi 5

deep Majister, Surpas 7

39. 40. QN Fruit: number of locules

(b) (*) MG predominantly two De Cayenne 1

(b) equally two and three Fehér 2

predominantly three Century 3

equally three and four Lamuyo, Sonar 4

G predominantly four and more Palio, PAZ szentesi 5


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CPVO N°

UPOV N°

Stage, Method


40. 41. QN Fruit: thickness of flesh

(b) (*) VG very thin De Cayenne, Macska sárga, Petit marseillais, Recio

1

(b) thin Banán, Carré doux extra hâtif, Doux très long des Landes

3

medium Fehér, Lamuyo 5

thick Andevalo, Bingor, Daniel, Topgirl 7

very thick Dragox Roda, Regolo, Solario 9

41. 42. QN Stalk: length

(b) (b) VG/MS very short Greygo, Golden calwonder 1

short Surpas, Yolo Wonder, Zenith 3

medium Fehér, Sonar 5

long De Cayenne, Sierra Nevada, Sweet banana

7

very long Farnese, Lipari, Oasis 9

42. 43. QN Stalk: thickness

(b) (b) VG/MS very thin De Cayenne, Doux très long des Landes, Macska sárga, Recio

1

thin Sweet banana 3

medium Doux italien, Surpas 5

thick Lamuyo, Trophy Palio 7

very thick Domingo, Galaxy, Paraiso 9

43. 44. QL Calyx: aspect

(+) (+) VG non enveloping Lamuyo, Sonar 1

(b) (b) enveloping De Cayenne, Sweet banana 2

44. (+)

45. (+)

QL Fruit: capsaicin in placenta

(b) (*) VG absent Sonar 1

G (b) present De Cayenne 9


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CPVO N°

UPOV N°

Stage, Method


45. 46. QN Time of beginning of flowering (first flower on second flowering node)

VG early Carré doux extra hâtif, Cupido, Fehér, Flaviano, Lito, Trophy

3

medium Lamuyo, Latino 5

late Daniel, Piquant d’Algérie, Zingaro 7

46. 47. QN Time of maturity

(+) (+) VG very early Koral, Macska sárga, Madison 1

early Fehér, Lady Bell, Topgirl 3

medium Lamuyo, Latino, Sonar 5

late Daniel, Doux d’Espagne 7

very late Cancun, California wonder 9

47. (+)

48. (+)

Resistance to Tobamovirus

47.1 (*)

48.1 (*)

QL Pathotype 0 (Tobacco MosaicVirus (0))

VG absent Doux italien, Piperade 1

G present Lamuyo, Sonar, Yolo Wonder 9

47.2 QL Pathotype 1 (Tobacco MosaicVirus (1))

VG absent Piperade, Yolo Wonder 1

present ’Tabasco’ (C. frutescens) 9

47.3 (*)

48.2 (*)

QL Pathotype 1-2 (Tobacco MosaicVirus (1-2))


G present Delgado, Festos, Novi, Orion 9

47.4 (*)

48.3 (*)

QL Pathotype 1-2-3 (Tobacco MosaicVirus (1-2-3))


G present Cuby, Tasty 9


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CPVO N°

UPOV N°

Stage, Method


48. (+)

49. (+)

Resistance to Potato Virus Y (PVY )

48.1 (*)

49.1 (*)

QL Pathotype 0

VG absent Yolo Wonder 1

G present Yolo Y 9

48.2 49.2 QL Pathotype 1

VG absent Yolo Wonder, Yolo Y 1

present Florida VR2 9

48.3 49.3 QL Pathotype 1-2

VG absent Florida VR2, Yolo Wonder, Yolo Y 1

present Serrano Criollo de Morenos 9

49. 50. QL Resistance to Phytophthora capsici

(+) (+) VG absent Yolo Wonder 1

present Chistera, Favolor, Phyo 636, Solario 9

50. 51. QL Resistance to Cucumber Mosaic Virus (CMV)


present Alby, Favolor 9

51. 52. QL Resistance to Tomato Spotted Wilt Virus (TSWV) – race P0


present Galileo, Jackal, Jackpot 9

52. 53. QL Resistance to Xanthomonas campestris pv. vesicatoria

(+) (+) VG absent Fehérözön, Yolo Wonder 1

present Aladin, Camelot, ECR-20R, Kaldóm, Kalorez, Lancelot, Pasa

9


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EXPLANATIONS AND METHODS

Explanations covering several characteristics

(a) Fruit characteristics which should be examined before maturity, i.e. before the first colour change

(b) Fruit characteristics which should be examined at maturity, i.e. after the time of the

first colour change

Ad. 2: Plant: length of stem The length of the stem is measured from the cotyledons to the first flower branch. Ad. 3: Plant: shortened internode (in upper part) Ad. 4: Varieties with shortened internodes only: Plant: number of internodes between the first flower and shortened internodes The tests should be done on plants which have not been pruned. The shoot system of pepper consists of main stems, which are branched off from the main axis and side shoots. Two growth types of the main stems can be distinguished: Growth type A: the main stems grow indeterminately; one or two flowers develop per node and shortened internodes never develop. Growth type B: after the first branching of the main axis, shorter internodes appear and the growth of the main stem ends in a bunch of flowers (it appears as if there are more than two flowers per node). Side shoots develop from the nodes on the main axis and on the main stems.


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Growth type A Growth type B Char. 3: Plant: shortened internodes (in upper part) absent present

Char. 4: Varieties with shortened internodes only: Plant: number of internodes between the first flower and shortened internodes

none (1)

one to three (2)

more than three (3)

flower node main stem side shoots

Ad. 9: Plant: height To be observed after a fruit set on several nodes. Poor fruit set may influence the vigour and thus the height of the plant. Ad. 13: Leaf: shape

1 2 3 lanceolate ovate broad elliptic Ad. 16: Leaf: profile in cross section

Main axis


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1 3 5 7 9 strongly moderately flat moderately strongly concave concave convex convex Ad. 18: Peduncle: attitude

1 2 3 erect semi-drooping drooping


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Ad. 27: Fruit: shape in longitudinal section

1 2 3 oblate circular cordate

4 5 6 square rectangular trapezoidal

7 8 9 moderately triangular narrowly triangular horn shaped


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Ad. 29: Fruit: sinuation of pericarp at basal part

1 3 5 7 9 absent or very weak weak medium strong very strong


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Ad. 30: Fruit: sinuation of pericarp excluding basal part

1 3 5 absent or very weak weak medium

7 9

strong very strong Ad. 38: Fruit: depth of interloculary grooves To be observed in the middle part of the fruit.


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Ad. 43: Calyx: aspect

1 2

non enveloping enveloping Ad. 44: Fruit: capsaicin in placenta The presence of capsaicin is observed by tasting the pepper flesh together with the locules, in the placenta area. Ad. 46: Time of maturity Maturity is reached at the first colour change of the fruit. Ad. 47: Resistance to Tobamovirus Maintenance of pathotypes Type of medium: On plants or dehydrated leaves (in deep-freezer or method BOS) Special conditions: Regeneration of the virus on plant material before inoculum preparation Execution of test Growth stage of plants: When cotyledons are fully developed or at “first leaf” stage Temperature: 20-25°C Growing method: Sowing and raising of seedlings in boxes or soil blocks in glasshouse


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Method of inoculation: Rubbing of cotyledons with a virus suspension Duration of test - Sowing to inoculation: 10 to 15 days - Inoculation to reading: 10 days Number of plants tested: 20 plants Genetics of virus pathotypes and resistant genotypes: The genetic resistance to Tobamoviruses is controlled by 5 alleles located on the same locus. The table below shows the relationship between virus pathotypes and resistance genotypes: Pepper Genotype reactions to Tobamovirus Pathotypes

Pepper Tobamovirus Pathotypes Virus: TMV ToMV PMMoV

Strain: U1 Feldman

P11 Obuda Pepper Mosaic Virus

P14 Samsun latens

Genotype / mark P0 P1-2 P1-2-3

L-L- S S S

L1L1 R S S

L3L3 R R S

L4L4 R R R

Legend: S = susceptible R = resistant TMV = Tobacco Mosaic Virus ToMV = Tomato Mosaic Virus PMMoV = Pepper Mild Mottle Virus Ad. 48: Resistance to Potato Virus Y (PVY) Maintenance of pathotypes Type of medium: On susceptible plants Special conditions: For the strain PVY(0): use the line TO72(A) For the strain PVY(1): use the line Sicile 15 For the strain PVY(1-2): use the line SON41 Execution of test


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Growth stage of plants: Young plants at the stage of developed cotyledons - first pointing leaf Temperature: 18-25°C Growing method: Raising of plants in glasshouse Method of inoculation: Rubbing of cotyledons with a virus solution Composition of the solution: inoculum: 4 ml extraction solution for 1 g infected leaves + 80 g activated carbon + 80 mg carborundum; extraction solution: buffer solution diluted 1/20 with 0.2% diethyl dithiocaremate of sodium (DIECA); buffer solution: (for 100 ml sterile water) 10.8 g Na2HPO4

+ 1.18 g K2HPO4 at pH 7.1-7.2

Duration of test Sowing to inoculation: 10 to 15 days Inoculation to reading: 3 weeks (2 weeks minimum, 4 weeks maximum) Number of plants tested: 20 plants Remarks: The test should not be conducted at high temperatures.

Standard varieties: Pathotype 0 Pathotype 1 Pathotype 1-2 Sensitive varieties: Yolo Wonder Yolo Wonder,

Yolo Y Florida VR2,* Yolo Wonder, Yolo Y

Resistant varieties: Yolo Y Florida VR2 Serrano Criollo de Morenos

* Florida VR2 can exhibit diffused and very late symptoms. Ad. 49: Resistance to Phytophthora capsici Scoring must be carried out under conditions of controlled infection: Maintenance of inoculum Inoculum and type of medium: Phytophthora capsici strain 101, to be cultivated on

V8 juice-agar (1%) in Petri’s dishes. Conduct of test


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Growth stage of plants: around eight-week old plants, grown in greenhouse

(stage: first flower bud) Temperature: 22°C Light: 12 hours/day Method of inoculation: Plants are cut just below the point of first branching. A disc of

mycelium of 4 mm in diameter should be used as inoculum. The disc is placed on the freshly cut stem. The top of the stem is wrapped with a piece of aluminium foil, to keep it wet. Infected plants are transferred to a growth chamber kept at 22°C.

Duration of test: From sowing to inoculation: between 6 and 8 weeks From inoculation to scoring: first scoring: 7 days

second scoring: 14 days final scoring: 21 days

Number of plants tested: 20 plants Scoring: The length of necrosis on the stem, induced by the fungus

development, is recorded once a week during 3 weeks, on each plant. The aluminium foil on the top of the stem should be removed 7 days after the inoculation. The first reading should take place immediately after the removal of the aluminium foil. Subsequent scoring should be made on the 14th and 21st day counting from the day of inoculation. The distance (in mm) between the lowest point reached by the necrosis and the top of the stem should be recorded.

Standard varieties: Susceptible: Yolo Wonder Resistant: Chistera, Favolor, Solario, Phyo 636 (given in the order

of their level of resistance) Ad. 50: Resistance to Cucumber Mosaic Virus (CMV) Maintenance of pathotypes Strain: Fulton Type of medium: On susceptible plants: Vinca rosea Special conditions: -


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Inoculum production: Crushing of 1g of fresh leaves of Vinca rosea in 4 ml of Phosphate buffer 0.03M pH 7 + DIECA (diethyl dithiocaremate de sodium) (1 for 1000) + 300 mg of activated carbon + 80 mg of carborundum

Execution of test: Growth stage of plants: Young plants at the stage of developed cotyledons. First leaf non-

pointing Number of plants: 20 plants Growing conditions: 22°C, 12 hours of light Growing method: Raising of plants in climatised room Method of inoculation: Mechanical rubbing of cotyledons with a virus solution, the plants are

kept in darkness for 48 hours Duration of test: From sowing to inoculation: 12 to 13 days From inoculation to reading: 3 readings at 10, 15 and 21 days after inoculation Standard varieties: Susceptible variety: Yolo Wonder Tolerant (T) or resistant (R) Milord (T) varieties: Vania (R) Ad. 51: Resistance to Tomato Spotted Wilt Virus (TSWV) –race P0 Maintenance of pathotypes: Type of medium: on susceptible pepper plants or freezing at -70 oC Execution of test: Growth stage of the plants: one or two leaves expanded Temperature: day: 20 oC, night: 20 oC Light: extra light in winter Growing method: glasshouse Inoculation medium 0.01 M PBS buffer with 0.1% sodium sulfite freshly added


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Method of inoculation: mechanical, rubbing with carborundum on cotyledons, Special conditions keep inoculum suspension cool during inoculation Duration of test: from sowing to inoculation: 20 days from inoculation to reading: 14 to 20 days Number of plants tested: 20 plants Remarks: beware of thrips; resistance will break down when temperature is

higher than 25 oC Standard varieties Susceptible: Bruinsma Wonder Resistant: Explorer, Prior Ad. 52: Resistance to Xanthomonas campestris pv. vesicatoria Maintenance of pathotypes Type of medium: PDA (Potato, Dextrose, Agar) medium Special conditions: 48 hours Xanthomonas campestris pv. vesicatoria culture. Adjusting inoculum concentration of bacteria-cellular 107. Execution of test Growth stage of plants: 6th to 8th true leaves Temperature: 24 °C night, 25°C day Relative humidity: 80% Light: 30 000 lx, day length 16 hours Growing method: Sowing in boxes in climate chamber or in glasshouse Method of inoculation: Infiltration into abaxial surface of a leaf in 13-15 mm diameter spots Duration of the test: 10-14 days Number of plants tested: 15 to 30 plants


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Remarks Genetics of bacteria pathotypes and resistant genotypes: Resistant varieties: Aladin, Camelot, ECR-20R, Kaldóm, Kalorez, Lancelot, Pasa


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LITERATURE GENERAL INFORMATION Palloix, A., Phaly, T., 1996: Histoire du piment: de la plante sauvage aux variétés modernes, PHM Revue Horticole, FR, no. 365; 41-43 Pochard, E., 1987: Histoire du piment et recherche, INRA Mensuel, FR, no. 29; 5-8 Pochard, E., Palloix, A., Daubeze, A.M., 1992: Le piment, Gallais, A. (ed.), Bannerot, H. (ed.), Amelioration des especes vegetales cultivees. Objectifs et critères de selection 420-434, INRA; Paris, FR Genetic Resources Daunay, M.C., Jullian, E., Dauphin, F., 2001: Management of eggplant and pepper genetic resources in Europe: networks are emerging, EUCARPIA, European Association for Research on Plant Breeding, Paris, FR, Genetics and breeding of Capsicum and eggplant 11th EUCARPIA Meeting, Antalya, TR, 2001 1-5 Disease Resistance Caranta, C., Palloix, A., Gébré-Sélassié, K., Marchoux, G., Lefebvre, V., Daubèze, A.M., 1996: Genomic organization of multi-virus resistance factors in pepper (Capsicum annuum): Co-localization between QTLs and major genes. Poster Lefebvre, V., Caranta, C., Moury, B., Pflieger, S., Daubèze, A.M., Blattes, A., Phaly, T., Nemouchi, G., Palloix, A., 1997: Status of the intraspecific molecular map of pepper: genome distribution of multiple disease resistance loci and defence genes, Sherago International Inc., New York, US, Plant and animal genome V, International Conference on the Status of Plant and Animal Genome Research, San Diego, US, 1997/01/12-16, 115 Pflieger, S., Lefebvre, V., Blattes, A., Caranta, C., Palloix, A., 1998: Candidate gene approach for identifying QTLs involved in pepper/pathogen interactions, EUCARPIA, European Association fo Research on Plant Breeding, Avignon, FR, Genetics and breeding of Capsicum and eggplant, 10th Meeting EUCARPIA, Avignon, FR, 1998/09/07-11, 245-248 Stacey, G. (ed.), Mullin, B. (ed.), Gresshoff, P.M. (ed.), Biology of plant-microbe interactions 8. International Symposium on molecular plant-microbe interactions, Knoxville (USA), 1996/07/12-19, 1 p., International Society for Molecular Plant-Microbe Interactions, Saint-Paul, US


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Potyvirus Parrella, G., Ruffel, S., Moretti, A., Morel, C., Palloix, A., Caranta, C., 2002: Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes, Theoretical and Applied Genetics, DE, vol. 105; 855-861 Ruffel, S., Dussault, M.H., Palloix, A., Moury, B., Bendahmane, A., Robaglia, C., Caranta, C., 2002: A natural recessive resistance gene against potato virus Y in pepper corresponds to the eukariotic initiation factor 4E (elF4E), Plant Journal, GB, vol. 32 no. 6; 1067-1075 CMV Caranta, C., Daubèze, A.M., Pflieger, S., Lefebvre, V., Thabuis, A., Blattes, A., Nemouchi, G., Phaly, T., Signoret, P., Palloix, A., 2001: Identification of quantitative trait loci involved in partial restriction of cucumber mosaic virus (CMV) long-distance movement in pepper, EUCARPIA, European Association for Research on Plant Breeding, Paris (FRA), Genetics and breeding of Capsicum and eggplant, 11th EUCARPIA Meeting, Antalya, TR, 2001 176-180 Caranta, C., Palloix, A., Lefebvre, V., Daubèze, A.M., 1997: QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host-cells, Theoretical and Applied Genetics, DE, no. 94; 431-438 Caranta, C., Pflieger, S., Lefebvre, V., Daubèze, A.M., Thabuis, A., Palloix, A., 2002: QTLs involved in the restriction of cucumber mosaic virus (CMV) long-distance movement in pepper, Theoretical and Applied Genetics, DE, vol. 104; 586-591 Phytophthora Lefèbvre, V., Palloix, A., 1995: Mapping QTL's affecting the resistance to Phytophthora capsici in pepper (Capsicum annuum), Scherago International Inc., New York, US, USDA, United States Department of Agriculture, Agricultural Research Service, Washington, US, International Conference on the Status of Plant Genome Research, Plant Genome 3, San Diego, US, 1995/01/15-19 58, USDA-ARS, Washington, US Lefebvre, V., Palloix, A., 1996: Both epistatic and additive effects of QTLs are involved in polygenic induced resistance to disease: a case study, the interaction pepper Phytophthora capsici Leonian, Theoretical and Applied Genetics, DE, no. 93; 503-511 Thabuis, A., Palloix, A., Pflieger, S., Daubèze, A.M., Caranta, C., Lefebvre, V., 2003: Comparative mapping of Phytophthora resistance loci in pepper germplasm: evidence for conserved resistance loci across Solanaceae and for a large genetic diversity, Theoretical and Applied Genetics, DE, vol. 106; 1473-1485


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Xanthomonas Márkus, F., Kapitány, J., Csilléry, G. and Szarka, J., 2001 b: Xanthomonas resistance In Hungarianspice pepper varieties. Int. Jour. of Hort. Sci., Voil. 7. No. 3-4. 69-72 Szarka, J. and Csilléry, G., 1995: Defence system against Xanthomonas campestris pv. vesicatoria. Eucarpia IXth Meeting on Genetics and Breeding of Capsicum and Eggplant. Budapest, Hungary, August 21-25. 184-187 TSWV Moury, B., Pflieger, S., Blattes, A., Lefebvre, V., Palloix, A., 2000: A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper, Genome, CA, no. 43; 137-142


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ANNEX II

TECHNICAL QUESTIONNAIRE

to be completed in connection with an application for Community Plant Variety Rights Please answer all questions. A question without any answer will lead to a non-attribution of an application date. In cases where a field / question is not applicable, please state so.

1. Botanical taxon: Name of the genus, species or sub-species to which the variety belongs and common name

Capsicum annuum L. SWEET PEPPER, HOT PEPPER, PAPRIKA, CHILI

2. Applicant(s): Name(s) and address(es), phone and fax number(s), Email address, and where appropriate name and address of the procedural representative

3. Variety denomination a) Where appropriate proposal for a variety denomination: b) Provisional designation (breeder’s reference):

European Union Community Plant Variety Office


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4. Information on origin, maintenance and reproduction of the variety 4.1 Method of maintenance and reproduction

a) i) hybrid ............................................................................................................. [ ] ii) open-pollinated variety ................................................................................. [ ] iii) parent line .................................................................................................... [ ] b) (ii) seed propagated ........................................................................................... [ ] (iii) vegetatively propagated ............................................................................. [ ] c) Other information on genetic origin and breeding method ............................... [ ]

4.2 Geographical origin of the variety: the region and the country in which the variety was bred or discovered and developed

5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristic in the CPVO Protocol; please mark the state of expression which best corresponds).

Characteristics Example varieties Note

5.1 (1)

Seedling: anthocyanin coloration of hypocotyl

absent Albaregia, Albena 1 [ ]

present Lamuyo 9 [ ]

5.2 (3)

Plant: shortened internode (in upper part)

absent California wonder, De Cayenne 1 [ ]

present Fehér, Kalocsai 601, Kalocsai 702 9 [ ]


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5.3 (20)

Fruit: colour before maturity

greenish white Blanc d’Espagne 1 [ ]

yellowish Fehér, Sweet banana 2 [ ]

green California wonder, Lamuyo 3 [ ]

purple Nigra 4 [ ]

5.4 (27)

Fruit: shape in longitudinal section

oblate Liebesapfel, PAZ szentesi, Topepo rosso 1 [ ]

circular Cherry Sweet 2 [ ]

cordate Daniel 3 [ ]

square Delphin, Yolo Wonder 4 [ ]

rectangular Clovis, Nocera rosso 5 [ ]

trapezoidal Delta, Piperade 6 [ ]

moderately triangular Fehér, Marconi 7 [ ]

narrowly triangular De Cayenne, Demon 8 [ ]

horn shaped Tauro 9 [ ]

5.5 (32)

Fruit: colour at maturity

yellow Golden calwonder, Heldor 1 [ ]

orange Ariane 2 [ ]

red Fehér, Lamuyo 3 [ ]

brown Brupa, Negral 4 [ ]

green Green6203 5 [ ]

5.6 (39)

Fruit: number of locules

predominantly two De Cayenne 1 [ ]

equally two and three Fehér 2 [ ]

predominantly three Century 3 [ ]

equally three and four Lamuyo, Sonar 4 [ ]

predominantly four and more Palio, Paz Szentesi 5 [ ]


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5.7 (44)

Fruit: capsaicin in placenta

absent Sonar 1 [ ]

present De Cayenne 9 [ ]

5.8 (47.1)

Resistance to Tobamovirus pathotype P0 (Tobacco Mosaic Virus (0))

absent Doux italien, Piperade 1 [ ]

present Lamuyo, Sonar, Yolo Wonder 9 [ ]

5.9 (47.3)

Resistance to Tobamovirus pathotype P1-2 (Pepper Mild Mottle Virus (1-2))

absent Piperade, Yolo Wonder 1 [ ]

present Delgado, Festos, Novi, Orion 9 [ ]

5.10 (47.4)

Resistance to Tobamovirus pathotype P1-2-3 (Pepper Mild Mottle Virus (1-2-3))

absent Piperade, Yolo Wonder 1 [ ]

present Cuby, Tasty 9 [ ]

5.11 (48.1)

Resistance to Potato Virus Y (PVY) pathotype P0

absent Yolo Wonder 1 [ ]

present Yolo Y 9 [ ]

6. Similar varieties and differences from these varieties:

Denomination of similar variety

Characteristic in which the similar variety is different1)

State of expression of similar variety

State of expression of candidate variety

____________ 1) In the case of identical states of expressions of both varieties, please indicate the size of the difference


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7. Additional information which may help to distinguish the variety 7.1 Resistance to pests and diseases absent present not tested

i) Resistance to Tobamovirus Pathotype 1 (char. 47.2) [ ] [ ] [ ]

ii) Resistance to Potato virus Y (PVY)

b) Pathotype 1 (char. 48.2) [ ] [ ] [ ]

c) Pathotype 1-2 (char. 48.3) [ ] [ ] [ ]

iii) Resistance to Phytophthora capsici (char. 49) [ ] [ ] [ ]

iv) Resistance to Cucumber Mosaic Virus (CMV) (char. 50) [ ] [ ] [ ]

v) Resistance to Tomato Spotted Wilt Virus (TSWV) (char. 51) [ ] [ ] [ ]

vi) Resistance to Xanthomonas campestris pv. vesicatoria (char. 52) [ ] [ ] [ ]

vii) Other resistances (specify) [ ] [ ] [ ]

7.2 Special conditions for the examination of the variety [ ] YES, please specify [ ] NO


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7.3 Other information [ ] YES, please specify [ ] NO

8. GMO-information required

The variety represents a Genetically Modified Organism within the meaning of Article 2(2) of Council Directive 2001/18/EC of 12/03/2001. [ ] YES [ ] NO If yes, please add a copy of the written attestation of the responsible authorities stating that a technical examination of the variety under Articles 55 and 56 of the Basic Regulation 2100/94 does not pose risks to the environment according to the norms of the above-mentioned Directive.


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9. Information on plant material to be examined 9.1 The expression of a characteristic or several characteristics of a variety may be affected by factors, such as pests and disease, chemical treatment (e.g. growth retardants or pesticides), effects of tissue culture, different rootstocks, scions taken from different growth phases of a tree, etc. 9.2 The plant material should not have undergone any treatment which would affect the expression of the characteristics of the variety, unless the competent authorities allow or request such treatment. If the plant material has undergone such treatment, full details of the treatment must be given. In this respect, please indicate below, to the best of your knowledge, if the plant material to be examined has been subjected to: (a) Microorganisms (e.g. virus, bacteria, phytoplasma) [ ] Yes [ ] No (b) Chemical treatment (e.g. growth retardant or pesticide) [ ] Yes [ ] No (c) Tissue culture [ ] Yes [ ] No (d) Other factors [ ] Yes [ ] No Please provide details of where you have indicated “Yes”: I/we hereby declare that to the best of my/our knowledge the information given in this form is complete and correct. Date Signature Name

[End of document]

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