Download - Supplementary Figures
Supplementary Figures
A
B
0
20
40
60
80
100
120
C DEX DEX+AG
cel
ls in
S+G
2/M
(% o
f con
trol
)***
***
*****
****
C DEX DEX+AG
***
***
***
**
***
*
MDA-MB-231
C DEX DEX+AG0
20
40
60
80
100
120
cel
ls in
Sub
G1
(% o
f con
trol
)
***
********
C DEX DEX+AG
****
**
***
**
Proliferation
Apoptosis
Supplementary Figure S1
siControlsiBRCA1
pcDNA3BRCA1wt
siControlsiBRCA1
pcDNA3BRCA1wt
Supplementary Figure S2
***
0
0.2
0.4
0.6
0.8
1.0
1.2
C DEX C DEX
siControl siBRCA1
mRN
A fo
ld in
ducti
on
***
******
***
tPa
C DEX C DEX
pcDNA3 BRCA1wt
0
0.2
0.4
0.6
0.8
1.0
1.2
C DEX C DEX
siControl siBRCA1
mRN
A fo
ld in
ducti
on
***
***IEX-1
C DEX C DEX
pcDNA3 BRCA1wt
***
***
***
***
***
C DEX C DEX
siControl siBRCA1
MKP-1
C DEX C DEXpcDNA3 BRCA1wt
***
***
***
0
2
4
6
8
10
mRN
A fo
ld in
ducti
on
G0S8
C DEX C DEX
siCtl siBRCA1
***
***
***
C DEX C DEX
pcDNA3 BRCAwt
***
***
0
1
2
3
4
5
mRN
A fo
ld in
ducti
on
B
C
A***
GRE
-Luc
fold
indu
ction
C DEX0
40
60
80
100
120
140
10.5 ***
***
***
***
C DEX
50
100
150
200
250
10.5
***
***
*
***
MDA-MB-231siControlsiBRCA1
pcDNA3BRCA1wt
MDABA
D E
0
0.5
1
1.5
2
2.5
3
3.5
C DEX
hGR
1A-L
uc fo
ld in
ducti
on
* *
***
0
0.5
1
1.5
2
2.5
3
C DEX
hGR
1B/C
-Luc
fold
indu
ction
***
***
si Control si BRCA1
BRCA1 silencing
BRCA1 overexpression
BRCA1
GR
Actin
GR P-Ser211
Ø DEX
siCo
ntro
l
siBR
CA1
siCo
ntro
l
siBR
CA1
GR
mRN
A le
vels
BRC
A1 o
vere
xpre
ssio
n co
nditi
on/C
tl co
nditi
on
0
0.5
1
1.5
2
0h 12h 24h 36h 48h
F
BRCA
1 m
RNA
leve
ls B
RCA1
ove
rexp
ress
ion
cond
ition
/Ctl
cond
ition
0
2000
4000
6000
0h 12h 24h 36h 48h
pcDNA3 BRCA1wt
hGR
1A-L
uc fo
ld in
ducti
on
**
***
***
***
0
1
2
3
4
5
C DEX
hGR
1B/C
-Luc
fold
indu
ction
**
***
***
***
0
1
2
3
4
5
C DEX
Supplementary Figure S3C
0
0.2
0.4
0.6
0.8
1
1.2m
RNA
fold
indu
ction
***
BRCA1 GR
***
si Control si BRCA1
Ø DEXpc
DN
A3
BRCA
1 w
t
pcD
NA
3
BRCA
1 w
t
BRCA1
GR
Actin
GR P-Ser211
