MOLD FORM OF FUNGUS
“SUPER FUNGUS”
FRUITING BODY OF PENICILLIUM
FRUITING BODY OF ASPERGILLUS
FRUITING BODY OF ASPERGILLUS
MICROCONIDIA
MACROCONIDIA OF ALTERNARIA
ARTHROSPORES
MACROCONIDIA OF FUSARIUM
MACROCONIDIUM OF M. CANIS
MACROCONIDIUM
HYPHA
MICROCONIDIA
TRICHOPHYTON
COILED HYPHA OF TRICHOPHYTON
SPORANGIUM
MUCOR
YEAST FORM OF FUNGUS
DERMATOPHYTE INFECTIONS
• GENERA– MICROSPORUM,– TRICHOPHYTON– EPIDERMOPHYTON
DERMATOPHYTE INFECTIONS
• LESIONS– HAIR- BROKEN, THICKENED– SKIN- CIRCUMSCRIBED, MAY BE
THICKENED OR ERYTHEMATOSUS
– NAILS- BRITTLE, CHALKY
DERMATOPHYTE INFECTIONS
• ANIMAL SPECIES – DOG- M. CANIS, M. GYPSEUM, T.
MENTAGROPHYTES– CAT-M. CANIS– CATTLE- T. VERRUCOSUM– PIG- M. NANUM– HORSE- T. MENTAGROPHYTES, T.
EQUINUM, M. GYPSEUM, M. CANIS
MICROSPORUM CANIS INFECTION
MICROSPORUM GYPSEUMINFECTION
MICROSPORUM CANIS INFECTION
MICROSPORUM CANIS INFECTION
TRICHOPHYTON MENTAGROPHYTES INFECTION
TRICHOPHYTON MENTAGROPHYTES INFECTION
TRICHOPHYTON INFECTION
TRICHPHYTON
TRICHOPHYTON
TRICHOPHYTON
SCALE/SCAB FROM LESIONTRICHOPHYTON OR DERMATOPHILUS
MICROSPORUM GYPSEUM INFECTION
MICROSPORUM GYPSEUM INFECTION
MICROSPORUM NANUM INFECTION
MICROSPORUM NANUM INFECTION
TRICHOPHYTON VERUCOSSUM
TRICHOPHYTON VERUCOSSUM
TRICHOPHYTON VERUCOSSUM
DERMATOPHYTE INFECTIONS
• DIAGNOSIS– CLINICAL SIGNS– WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC
RINGWORM OR DERMATOPHYTOSIS
RINGWORM OR DERMATOPHYTOSIS
RINGWORM OR DERMATOPHYTOSIS
DERMATOPHYTE INFECTIONS
• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC
DERMATOPHYTOSISWOOD’S LIGHT POSITIVE
FLUORESCENCE
FLUORESCE
DERMATOPHYTE INFECTIONS
• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC
HYPHA IN KOH DIGEST OF SKIN
HYPHAE IN KOH DIGEST OF SKIN
HYPHA IN KOH DIGEST OF HAIR
DERMATOPHYTE INFECTIONS
• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE
•DTM, FUNGASSAY- TURNS RED •BHICC
DERMATOPHYTE INFECTIONS
• DIAGNOSIS- CULTURE– ALCOHOL PREP BEFORE SCRAPING– INOCULATE SURFACE– WATCH FOR RED COLOR WITH WHITE TO TAN
SURFACE GROWTH ON DTM BEFORE 14 DAYS– RED COLOR IS PRESUMPTIVE- DO TAPE MOUNT
TO LOOK FOR HYPHAE, MICRO-/MACROCONIDIA WHICH MAY BE CHARACTERISTIC OF A DERMATOPHYTE
DTM OR FUNGASSSAY MEDIUM
DTM OR FUNGASSAY
POSITIVE CULTURES
NEGATIVE CULTURES
NEGATIVE CULTURES
DTM OR FUNGASSAY
NEGATIVE POSITIVE
DTM OR FUNGASSAY
POSITIVEFALSE
POSITiVE
POSITIVE DTM OR FUNGASSAYTOP OF PLATE
POSITIVE DTM OR FUNGASSAYBACK OF PLATE
POSITIVE DTM OR FUNGASSAY
FALSE POSITIVE DTM OR FUNGASSAY
DTM OR FUNGASSAY
NEGATIVE
POSSIBLEPOSITIVE
DTM OR FUNGASSAYPOOR SPECIMEN
DTM OR FUNGASSAYPOOR SPECIMEN
MACROCONIDIUM OF M. CANIS
MACROCONIDIA MICROSPORUM GYPSEUM
MACROCONIDIA MICROSPORUM NANUM
TAPEMOUNT OF TRICHOPHYTON
HYPHA
MICROCONIDIA
TAPEMOUNT OF TRICHOPHYTON
MACROCONIDIA
HYPHA
MICROCONIDIA
TAPEMOUNT OF TRICHOPHYTON
COILED HYPHA
HUMAN DERMATOPHYTE INFECTION
HUMAN DERMATOPHYTE INFECTION
HUMAN DERMATOPHYTE INFECTION
HUMAN DERMATOPHYTE INFECTION
HUMAN DERMATOPHYTE INFECTION
HUMAN DERMATOPHYTE INFECTION
SKIN INFECTIONS
• DERMATOPHILUS– STREPTOTRICHOSIS
– DIAGNOSIS BY DIRECT EXAMINATION
SKIN INFECTIONS
• DERMATOPHILOSIS- DIAGNOSIS– REMOVE SCABS- ZOONOTIC ORGANISM – PLACE SCABS IN TEST TUBE. – ADD ONLY ENOUGH WATER TO BREAK UP SCAB – THE SLIGHT AMOUNT OF SUPERNATANT SHOULD
BE TURBID AFTER THE SCABS ARE BROKEN DOWN.
– SPREAD DROP OR TWO OF SUPERNATE OVER A 1 X 3 CM AREA OF SLIDE. G. AIR DRY, FIX IN ALCOHOL
SKIN INFECTIONS
• DERMATOPHYOSIS- DIAGNOSIS– STAIN WITH GIEMSA FOR 15-25
MINUTES – LOOK FOR RAILROAD TRACKS UNDER
OIL IMMERSION
MICROSPORUM CANIS INFECTION
