Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S83
Effect of a formulation of selected dairy startercultures and probiotics on microbiological,chemical and sensory characteristics of swinedry-cured sausages
Paola Sechi 1,∗, Maria Francesca Iulietto 1, SaraMattei 1, Giovanna Traina 2, Michela Codini 2,Beniamino Terzo Cenci Goga 1
1 Dipartimento di Medicina Veterinaria, Perugia,Italy2 Dipartimento di Scienze Farmaceutiche, Perugia,Italy
E-mail address: [email protected] (P. Sechi).
Aim: Evaluation of a “formulation of selected dairy starter-culturesand commercial probiotics” in the production of nitrite-free fer-mented pork sausage produced in a small-scale plant, and theireffect on microbiological, physico-chemical and sensorial proper-ties of the products. Conditions investigated: (i) “formulation ofselected dairy starter-cultures and commercial probiotics” (PRO)vs no starter (NO-PRO); (ii) “specific ripening conditions” (SPEC) vs“classic ripening conditions” (NO-SPEC); (iii) nitrate (NIT) vs nitriteand nitrate-free sausages (NO-NIT). Two trials: (1) NIT, PRO vs NO-PRO, SPEC vs NO-SPEC: here starter cultures and probiotics had asimilar behaviour in SPEC and in NO-SPEC, sensory properties wereimproved in salami added with PRO both in SPEC and in NO-SPECand pathogens control was greater in salami added with PRO inboth ripening conditions; (2) NIT, NO SPEC vs SPEC, PRO vs NO-PRO:here starter cultures and probiotics showed a correct evolutionwhile pathogens and hygiene-indicators were reduced throughoutripening both in SPEC and in NO SPEC. PRO in SPEC did prevent thegrowth of safety and hygiene indicators, greatly reduced the rateof isolation of pathogens and increased the acceptability of salami,demonstrating the practicability of producing fermented sausageswithout nitrites yet preserving the original flavour and aroma.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.283
Bacteriophages useful for elimination ofCronobacter strains in infant formulae
Hana Drahovska ∗, Michal Kajsik, LuciaOslanecova, Jan Turna
Department of Molecular Biology, Faculty of NaturalSciences, Comenius University, Bratislava, Slovakia
E-mail address: [email protected] (H. Drahovska).
Cronobacter spp. are gram-negative opportunistic bacteria belong-ing to Enterobacteriaceae family. This pathogen is responsible forserious bacteraemia, necrotizing enterocolitis and meningitis inneonates and infants, with fatality rates as high as 80%. Powderedinfant milk formula has been implicated as the vehicle of transmis-sion. Bacteriophages can be used in phage therapy of Cronobacterinfections and also in the protection of infant formula. The aim ofthe work was to characterize Cronobacter specific bacteriophagesfor application in food control. Five phages were isolated fromsewage water. Two of them (Pet-CM-3 and Dev-CS701) possesseda broad host specificity as they lysed 78% and 98% tested strains.Based on genome sequence they belonged to the T4-like groupof Myoviridae family. The Dev2 bacteriophage had host specificitylimited to C. turicensis serotype CT O:1 strains. Its genome was sim-ilar to K1F phage belonging to T7-like group of Podoviridae family.Two other mutually similar phages (Dev-CT57 and Dev-CD23823)also belonged to T7-like group of Podoviridae. They were able toinfect more than 80% Cronobacter strains. In the study, the ability of
all phages to kill host bacteria in reconstituted infant milk formulawas also confirmed.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.284
Identification and characterization of highGABA and low biogenic amine producingindigenous yeasts isolated from Koreantraditional fermented Bokbunja (Rubuscoreanus Miquel) wine
Nho Eul Song ∗, Sang Ho Baik
Department of Food Science and Human Nutrition,and Research Institute of Makgeolli, ChonbukNational University, Jeonju, Republic of Korea
E-mail address: [email protected] (N.E. Song).
The contribution of gamma-aminobutyric acid (GABA) producingyeasts and reduction of the amines which has dose-dependenttoxicity to human is not well investigated on wine fermentation.The aim of this research was screening of the GABA releasing andlow biogenic amine (BA) producing indigenous yeasts adapted tothe Korean traditional Bokbunja wine. Among 200 strains, sevenyeast-like strains which produced over 0.2–0.7 g/L of GABA wereselected as high GABA producing strains. The selected strains wereidentified as Saccharomyces cerevisiae by 26S rRNA sequence anal-ysis. Especially, S. cerevisiae JBCC A74 strain was shown to haveboth high alcohol synthesis (above 10%) and high synthetic abilityof GABA on the Bokbunja (0.33 g/L). The BA analysis on the Bok-bunja wine fermented by S. cerevisiae JBCC A21, B40, B43 and B197showed lower BA concentration than control wine. Cadaverine wasnot detected and serotonine concentration was approximately 3to 5-fold reduced in wine. The alcohol contents in the final Bok-bunja wine were 9–12%. In this research, GABA enriched as wellas histamine-free Bokbunja wine was manufactured by functionalindigenous S. cerevisiae. Our finding could contribute to furtherfunctional wine industry by adapting as a good wine fermentationstarter.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.285
Prevention of TNBS-induced colitis by probioticsupplement in mice
Gabriele Brecchia 1,∗, Francesca Rappa 2,Francesco Cappello 2, Giovanna Traina 3
1 Department of Veterinary Medicine, University ofPerugia, Perugia, Italy2 Department of Experimental Biomedicine andClinical Neurosciences, University of Palermo,Palermo, Italy3 Department of Pharmaceutical Sciences, Universityof Perugia, Perugia, Italy
E-mail address: [email protected] (G. Brecchia).
Probiotics may protect against inflammatory bowel disease (IBD).The aim of this study was to investigate whether a commercialprobiotic mixture prevented gut inflammatory disease. CD-1 micereceived daily a probiotic mixture of Lactobacillus casei and Bifi-dobacterium lactis (Citogenex, Bromatech, Milano, Italy) for 1, 2 or 3weeks by gavage. Colitis was induced by intrarectal administrationof trinitrobenzene-sulfonic acid (TNBS) at the end of the treatmentperiods. Large bowel was stained and assessed for histological alter-ations, according to a scoring system. Large bowel was also assessedfor apoptosis by TUNEL assay. TNBS induced inflammation and
