Transcript
Page 1: Artificial insemination steps for carp

Firas Abdul Malik

Page 2: Artificial insemination steps for carp

Parent Selection:Parent Selection:1. Isolating parents have a good

characteristics according to the morphological shape.

2. Isolating female from male after check the sexual maturity.

Page 3: Artificial insemination steps for carp

Transporting the male & female to the Transporting the male & female to the hatchery ponds:hatchery ponds:1. Keeping it from light &

noise.2. Watching the fish

continuously & measuring the water temperature for readying it for drugging & injection by hormone.

3. Weighing male & female alone for determined the hormone quantity.

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Preparing the parents for drugging Preparing the parents for drugging before spawning.before spawning.

1. Preparing carnations matter or oil ((القرنفل.

2. Preparing special ponds for drugging.

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Parent drugging:Parent drugging:1. Using carnations matter

(powder) -50g/100 liter water, or using carnation oil (1ml/10 Liter water).

2. Watching the fish until the end of drugging process.

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Hormone preparing:Hormone preparing:1. Determined the hormone quantity

after determining the fish weight.2. Taking the hormone in granulates

shape.3. Well bray the quantity in ceramic

mortar (هاون خزفي). 4. Adding distilled water to the

braying hormone.5. The quantity of water =0.25ml/Kg

fish weight to the first & second injection .

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Page 8: Artificial insemination steps for carp

Female injection by hormone:Female injection by hormone:1. Prepare female for injection process.2. Inject the drug fish after well fix it.3. Inject fish below pectoral fin or above

lateral line (( طDوق الخDة أو فDة الكتفيDتحت الزعنف.الجانبي

4. Give the first dose (10%) from dissolver hormone in 0.25 ml-physiological dissoluble/Kg fish.

5. Giving (90%) from hormone as the second dose after 12 hours from the first dose dissolver hormone in 0.25 ml-physiological dissoluble/Kg fish.

6. Male take only one dose of hormone within the second dose of female.

7. Fish weight must be same.

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Sewing the anus:Sewing the anus: Anus sewing by using

special sewing needle after disinfect it and using disinfect abb to prevent eggs exits.

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Watching the parents:Watching the parents:1. Continually watch parents.2. Must continue watch

parents after 8-9 hours according to the water temp.

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Eggs collection:Eggs collection:1. Egg collects by light

massage for abdomen area to anus direction.

2. Egg collects in dry plastic containers.

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Page 13: Artificial insemination steps for carp

Taking the male semen:Taking the male semen:1. Semen collects in glass jug.2. Prefer to collect semen from three

males(3) in three jugs.3. Adding the semen to the egg in (10ml)/

Kg of dry egg for fertilization.4. Mixing the semen with eggs to finalize

the fertility process using swan or turkey feathers روميDيك الDدDط او الDريش الب for 2to 3 minutes by adding few water.

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Eggs fertilization:Eggs fertilization:

1. Add few water to the egg container.

2. Mix egg continually to fertilize it.

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Prepare egg washing liquid:Prepare egg washing liquid:

1. Take (10) liter from hatchery water.

2. Add (30g) urea.3. Add (40g) salt free from iodine.

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Washing fertilized egg by water:Washing fertilized egg by water:1. Adding water for special liquid for egg

covering up.2. Mix egg continuously between (50-60)

minutes. 3. Change water continuously after (3-4)

minutes. 4. Continue process until removing the

egg limyلزوجة. 5. Increasing the egg size and never

adhesion indicate the final of process.

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Washing egg by tenen material:Washing egg by tenen material:1. Add (1g) from tenek acid to (1liter)

water.2. Prepared quickly throw washing

period-between (40-50 minutes).3. Tenek acid solid the eggs & increase

the oil materials in internal membrane.4. Wash egg by water to riddance from

tenek acid.

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Transport the zygote to the Zog Transport the zygote to the Zog jars: jars:

1. But between (150-200g) zygote for each zog jar.

2. But gradually zygote inside zog jar quit after full zog jar to the half by water.

3. Determine interred water flow rate to zog jar slowly for the first (12) hours.

4. increase water flow rate (1-2) liter/minute.

5. Another types of carp differ from egg quantity (50-70g) of egg for each zog jars with decrease water flow rate to the minimum value.

6. disinfect the eggs by malachite green one time after (12) hours and stop in when egg hatching start.

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Egg hatching period:Egg hatching period:1. Egg still in zog jars between (3-5)

days according to the water temp.2. Looking after the eggs for exiting

the destroyed eggs. 3. Disinfect the egg by malachite green

twice a day (daily & nightly).4. You can see the fry at the zog jar

sides.

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Fry transporting to dry nurse Fry transporting to dry nurse (incubator):(incubator):

1. Fry hatch in the incubator for 24 hours without artificial feeding because this fry depend on yolk sac for feeding.

2. Put between (400-500) fry for each incubator. 3. Fry feeds artificially when they can swim horizontally.

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Fry feeding inside incubators:Fry feeding inside incubators:1. Fry feeds on emulsifyلوقDبيض المسDفار الDتحلب صDمس of boiled egg.2. One Egg yolk enough for two incubators.3. Feeding continue one time over (4) four hours for (3) three

days.

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Ground ponds preparing for fry Ground ponds preparing for fry culture:culture:

1. before fry culture check pond inlet and outlet for leaking.

2. Check for a while before fry transfer to avoid aquatic organisms & insects grows which harm fry.

3. Gradually increase pond depth to (50-70cm).

4. Preferring fry culture in cages to easy control it.

5. The quantity of water must be increase after (10-15) days.

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Fry feeding in ponds:Fry feeding in ponds:1. Fry feeds on soybean emulsify

on the first days.2. Emulsify quantity about

(5mg/1 million fry).3. Emulsify quantity increased

after three days.4. After (20) days of culture, fry

weighing (0.5-1g) feeds on ground brew diet.

5. When fry reach (0.5-1g) weight prefer to transport it to another big ponds with low density for marketing purpose.

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Continue growing process:Continue growing process:1. Fishing the fish & record growth rate by recording length &

width.2. Re-record these measurements to know the grow increase to

determined the enough diet.3. Analyze the physical & chemical parameters of water such

as temperature, pH & oxygen.4. These processes must done before and after fry culture and

continue daily.


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