do's and dont's in the lab
TRANSCRIPT
Clinical Laboratory’s Do’s and Dont’sBSMLS 2D2013-2014
MEMBERS:
Bautista, Charles Christian H.Brioso, Princess Michelle
Cabinian,JaymiluCabral, Teresa Liway A.Garcia, Maria Karissa A.Gonzales, Jemmuel E.Lontoc, Shelah Mae H.Macatangay, SophiaTatlonghari, Edmon H.Villacorta, Jon Vincent B.
Lab Safety
Why does it matter?
Safe working protects:YouOther lab workersCleanersVisitorsYour work
Protecting yourself (PPE)
Fasten laboratory coats
Don’t wear sandals or open shoes
Tie back long hair
Gloves
Use the correct ones for the job you will be doing
Remove gloves before using instruments, telephone, and leaving the laboratory
Laboratory hygiene
Do not eat, drink or smoke in a laboratory
Do not apply cosmetics
Do not touch your face, mouth or eyes
Do not suck pens or chew pencils
Glassware SafetyDo not dispose broken glassware in a regular trashcan.
When pouring liquids into glassware, make sure the container you are pouring into is resting on a table at least a hands breadth from the edge.
Spillages
Clear up spillage promptly
Dispose of any hazardous material as toxic waste“Messy workers are usually poor workers!!”
Electrical Hazard
Always do a visual check on electrical equipment before use, look for obvious wear or defects
Waste Disposal
Do not put materials down the drain or in with normal waste unless authorised to do so
Wear appropriate PPE when handling clinical waste
Chemical Safety Always add acid to water Pour from large containers to
smaller ones. Do not taste any chemical. Do not put your nose over
the container and inhale the fumes.
Acid
Water
Fire HazardUse the smallest quantities of flammable solvents practicable.
Do not use a Bunsen burner in any area where flammable solvents are handled.
Risk of Infection If exposed to a bio hazardous specimen: Immediately wash the area with soap and H2O
Flush splashes to nose, mouth or skin with H2O
Irrigate eyes with clean H2O or saline
Perform background tests
Aseptic TechniquePerform proper hand washing
Reading Volume Measurements
All readings must be made with the eye on level with the meniscus to avoid parallax error.
Pipetting techniquesHold the pipet between the thumb and forefinger
Do not pipet using your mouth, use a rubber aspirator
Operating a CentrifugeDo not remove the cover of the centrifuge until it has come to a complete stop
Make sure the loads are balanced
Using microscopeGently wipe the lens with lens paper or muslin cloth
Always carry the microscope with one hand supporting the base and the other hand around the arm.
Sharps HazardsAlways use sterile,disposable needle
Never recap a needle
Dispose sharps in puncture-resistant, leak-proof container
Order of Draw and Inversion
Always follow the correct order of draw of tubes and tube inversionPurple(8x)Blue(3-4x)Red glass (0x)Red plastic (5x)
SyringesAlways transfer immediately blood drawn from syringe into an evacuated tube
Transporting samples to the laboratory
Deliver samples to the laboratory within 45 minutes and centrifuge on arrival
Specimen Collection and Handling Procedures
Allow samples to clot fully before centrifugation to complete separation of the cells and serum
Protect bilirubin, Vit A and carotene samples from light
Laboratory CleanlinessDo maintain good lab cleanliness - it’s for your own safety and as well as for others!
Thank you!