does dna methylation facilitate phenotypic plasticity in marine invertebrates?
DESCRIPTION
Presentation given at the Friday Harbor Laboratories on August 13th, 2014TRANSCRIPT
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?Steven RobertsSchool of Aquatic and Fishery Sciencesrobertslab.info@sr320
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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
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commercially important traits
Lab Background
commercially important traits
modified from
Lab Background
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modified from
modified from
Today
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1
23
Histone Modification
Epigenetics
short RNAs
DNA Methylation
Photo credit: Flickr, Creative Commons, he-boden
Epigenetics
Epigenetics
m
m
DNA Methylation
Function?
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
Non-Vertebrates?
landscape and function is very different than what is observed in vertebrates
Absent in severalmodel
organisms
Function?Oysters?
mosaic
associated with gene bodies
Roberts and Gavery 2012
in silico analysis
Roberts and Gavery 2012
in silico analysis
Roberts and Gavery 2012
in silico analysis
Two Lineages-
Sperm + Larvae
(72h & 120h)
Claire Olson
Whole Genome BS-Seq
Claire Olson
Differentially Methylated Loci predominant in Transposable Elements
Claire Olsongithub.com/sr320/olson-dev
Summary
• Sparsely (~16 %) methylated genome
• Gene body methylation• Function specific
• TEs are not hypermethylated across genome• Preliminary evidence indicates DMRs are predominant in TEs
Role?
In species that experience a diverse range of environmental conditions, processes have evolved to
increase the number of potential phenotypes in a population in order to improve the chances for an
individual’s survival.
Roberts and Gavery 2012
in silico analysis
Stochastic Variation
housekeeping response to change
Mackenzie Gavery
housekeeping
response to change
Targeted Regulation
Mackenzie Gavery
Mackenzie Gavery
Transposable Elements providing increased diversity?
modified from
Beginning totest these
Hypotheses
Very new dataEnvironment and gene expression
stochastic or targeted?
Very new dataEnvironmental impact (Estrogens)
Very new dataOcean AcidificationSelectionEnvironmental Impact
Katie Lotterhos
Very new dataHeritabilityPlasticityLocal Adaptation
Geneticsversus
Epigenetics
Common Garden Experiment
Acknowledgements
Mackenzie GaveryClaire Ellis
Sam White
Bill HoweDan Halperin
EPASTARDNA methylation
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