dna repair pathways
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DNA Repair Pathways
043_
12_K
M
Copyright © 2012 Abcam, All Rights Reserved. The Abcam logo is a registered trademark.All information / detail is correct at time of going to print.
Polε
Polε
Polε
Msh2 Msh6 (MutSα)
Msh2 Msh6 (MutSα)
MutLα endonucleaseactivity andExo1 remove mismatch by 5’ to 3’ resection
RFC/PCNA andendonucleaseactivity of MutLαallow Exo1 to resect DNA 3’ of themismatch
Single base pair mismatchon lagging strand
Single base pair mismatchon leading strand
≥ 1 base pair insertion or deletionSingle base pair mismatch
Replication Resumes
PCNA
Mlh1 Pms2 (MutLα)
and
Msh2 Msh3 (MutSβ)
Exo1
Exo1
PCNA
Polδ/ε
Polε
5’
3’
3’
5’
3’5’
DNA Pol
5’
DNA Pol
DNA Pol3’
5’
PolδPolδδPolα
P
Polα
PolδPolδδPolα
PolδPolδδPolα
PolδPolδδPolα
RPA
XPG
UVDNA crosslinkers
Transcription coupled repairGlobal genome repair
Transcriptionblock PARP1
RPA
and
PCNA
XPE
HR23BXPC
HR23BXPC
HR23BXPC
TFIID
TFB5XPBXPD
TFIID
TFB5
RPPAA
XPBXPD
Polδ/εRFC
DNALigase
XPG
XPA
ERCC1XPF
ERCC1
XPF
RPA XPA
CSA
CSB
RNA Pol I/II
BRCA1 P P
P P P
Mre11Rad50Nbs1
Rad51BCDXRCC2/3
P
P
P
P
P
P
P
P
P
P
P
P
P
P P
P
P
BRCA2
BRCC36
BRCA2
Bard1
Ubc13
BRCA1
ATM
Rad52
Rad52
Rad51BCDXRCC2/3
BRCA2
P
BRCA1Rad52
ATM
Ionizing radiationGenotoxic chemicalsFree radicalsMechanical stress
Homology searchand D-loop formation
Homologous chromosome
DNA synthesis
Rad51 monomers
histones
γ-H2AX
PPγ-H2AXH2A
Poly ubiquitinon H2A
3’
3’
3’
BR
3’
3’
5’
5’
5’
5’
5’ 5’
5’5’
3’
Resection
Resection
SMC1
Mre11Rad50Nbs1
ATM
MRNcomplex
Hollidayjunction
resolvase
ATM
CtIP
CtIP
Rad54
Rad54
Rad51BCDXRCC2/3
ATM
Mdc1
DNAPol
DNAPol
Ligation and formationof Holliday Junction
Non-crossover(cut both junctionsvertically or horizontally)
Crossover(cut both black orboth red arrows)
DNALigase1
Rap80
Abraxas P
Rnf8
ATM
Caesin Kinase2
MRNcomplex
Nucleotide Excision Repair
P
P
P
P
P
P
P P
ATM
Polβ
Mre11Rad50Nbs1
XRCC1
Artemis
DNALigase 3
Ionizing radiationGenotoxic chemicalsFree radicalsMechanical stress
Homologousrecombinations
Strand openingand processingby exonucleaseactivity of WRN
No deletionAccurate repair
DNA ligation
Hairpin DNA cutby Artemis
Flap excision
Microhomologymediated strandannealing
Ku / Lig4 independentpathway
Strand tethering
Hairpin DNAends
Ku / Lig4 dependentpathway
Histones
PP P
Histones
γ-H2AX
H4K20Me2
XLF
PNKP
Mre11Rad50Nbs1
Ku70
Ku80
Ku70
Ku80
P
P
DNA PKcs
DNA PKcs
Ku70
Ku80
Ku70
Ku80
P
P
DNA PKcs
DNA PKcs
Ku70
Ku80
Ku70
Ku80
DNALigase 4
WRN
Fen1
WRN
Short deletionInaccurate repair
PARP1
PARP1
XRCC4
Rag 1/2
V JD
V JD
ATM
ATM ATM
53BP1
Nonhomologous End-Joining
Homologous Recombination Mismatch Repair
Produced in collaboration with James Haber and Farokh Dotiwala, Brandeis University.
UbqUbq
Ubq
Ubq
P
qPP
P P P P P
P
P
P P P P P
DNA crosslinkers, CisplatinMitomycin C, Fork stalling agents
Stalledreplicationfork
FANCD2/I
γ-H2AX
Repaired by NER
P
Histones
Repaired by HR
Translesion bypassby Rev1/3
Translocation tofork stall site
3’
3’
3’
5’
5’
5’
5’
REV1/3
3’
3’
3’
5’
5’
5’
5’
FANCB
FA100
FANCL
FANCM
FANCGFANCA
FAAP24
FANCEFANCF
FANCC
FANCB
FA100
FANCL
FANCM
FANCGFANCA
FAAP24
FANCEFANCF
FANCC
UBE2T
Chk1
ATR
FANCIFANCD2
ATR
E22TT
E1 Ligase
REV1/3
ATRIP
ATRIP
Polδ/ε
Polδ/ε
Polδ/ε
DNA Strand Crosslink Repair
Featured DNA Repair antibodies
Anti-BrdU - Proliferation Marker antibody (ab8152)
Hela cells stained with ab8152 at 1/100dilution.
Trial-sized aliquots of your favorite antibodies
Optimize before you commit.• DNA damage response• Histone modifications
• RNA polymerase II
For more details visit: www.abcam.com/panels
gamma H2A.X(phospho S139) antibody (ab2893)
Pachytenespermatocytes stainedwith ab2893 at 1/200.
DAPI
BrdU
Merge
043_12_KM DNA Repair Poster:Layout 1 27/02/2012 07:26 Page 1