dna extraction from strawberries

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DNA Extraction from Strawberries

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DNA Extraction from Strawberries. Lab Safety and Tips. Wear Goggles Important to have clean glassware. Follow directions CAREFULLY or you will not get good results. I DNA. - PowerPoint PPT Presentation

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Page 1: DNA Extraction from  Strawberries

DNA Extraction from Strawberries

Page 2: DNA Extraction from  Strawberries

Lab Safety and Tips

• Wear Goggles• Important to have

clean glassware.• Follow directions

CAREFULLY or you will not get good results.

Page 3: DNA Extraction from  Strawberries

I DNA

• Single molecules of DNA are long and stringy. Each cell of your body contains six feet of DNA, but it's only one-millionth of an inch wide.

Page 4: DNA Extraction from  Strawberries

Chromosomes

• Strands of DNA molecules coated with protein

Page 5: DNA Extraction from  Strawberries

Start with a Strawberry

• Easy to pulverize• Already has

enzymes• Large genome

(lots of chromosomes)

Page 6: DNA Extraction from  Strawberries

Add Detergent

• Detergent/shampoo captures protein and releases DNA

Page 7: DNA Extraction from  Strawberries

Mashing Strawberries in a thick Ziploc bag

Page 8: DNA Extraction from  Strawberries

Careful!!

You don’t want Bubbles!DNA clings to the bubbles. 

Page 9: DNA Extraction from  Strawberries

Salt

• The salt in the extraction buffer keeps the DNA molecules together and separates them from the protein.

Page 10: DNA Extraction from  Strawberries

Filtering the mixture will remove cell debris and organelles.

Page 11: DNA Extraction from  Strawberries

95% Cold Alcohol

• Alcohol separates the DNA from the other components of the cell

Note: slowly pouring down the side of the test tube

Page 12: DNA Extraction from  Strawberries

Good Tip

• Be careful to pour down the side of the container to form a layer on top instead of mixing the two layers.

Page 13: DNA Extraction from  Strawberries

ETHANOL MUST BE COLD!

Page 14: DNA Extraction from  Strawberries

• The DNA will rise into the alcohol layer and can be ‘spooled’ onto the wooden stick be carefully swirling the stick at the interface of the two layers.

Page 15: DNA Extraction from  Strawberries

DNA will look like snot

• White• Stringy• You are viewing • the DNA and protein

Page 16: DNA Extraction from  Strawberries

When DNA extractions are performed, you can expect three

basic results.

• No DNA • DNA appears fluffy which means

it has sheared in the extraction process

• DNA appears as thin threads.

Page 17: DNA Extraction from  Strawberries

Does you DNA look like this?

Page 18: DNA Extraction from  Strawberries

If not…Possible Errors

• Impurities could have easily been introduced during any step, which may have altered the outcome.

• Disturbing the solution as the DNA precipitated could cause poor results (probably due to the strands of DNA breaking up).

• Not allowing enough time for each step.