curcumin induces both apoptosis and autophagy in human papillary thyroid carcinoma bcpap cells zhang...
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Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells
Zhang Li Jiangsu Institute of Nuclear Medicine
Wuxi, Jiangsu, China
Contact: [email protected]
10th AOTA CongressBALI, INDONESIA, 2012
Curcumin
An acitve polyphenol derived from the plant turmeric
low toxicity
anti-inflammatoryantioxidantantiangiogenic antitumour
Chemical structure of curcumin
Curcumin Thyroid cancer ?
Apoptosis and Autophagy
Apoptosis –type I programmed cell death
Autophagy- type II programmed cell death
- a self-degradative process
- cope with stresses
- required in apoptotic-deficient cells
Three types of autophgay:
- Microautophagy- Chaperone-mediated autophagy- Macroautopahgy
Part I
Crucumin suppresses BCPAP cells growth
A B
Effects of curcumin on cell viability
Effects of curcumin on cell morphology
PI/Hoechst 33342 staining
A B
Part II
Curcumin induces apoptosis in BCPAP cells
Effects of curcumin on pro-apoptotic and anti-apoptotic proteins
A
B
Effects of curcumin on loss of mitochondrial membrane potential (MMP)
Caspase-dependent apoptosis in
curcumin-treated cells
Part III
Curcumin induces autophagy in BCPAP cells
Induction of autophagy in curcumin-treated cells
A
B
The FASEB JournalJune 2003vol. 17 no. 9 1165-1167
RF
P-L
C3
Curcumin (mM)
0 50
C
Induction of autophagy in curcumin-treated cells
Effects of curcumin on the phosphorylation of AKT pathway
A
B
Effects of curcumin on the phosphorylation of MAP kinases
Curcumin selectively induces autopagy in tumor cells
A
B
Curcumin seletively induces cancer cell death
A B
Dynamic distribution of curcumin in BCPAP cells
Enlarged
6 h
12 h
24 h
Effects of curcumin on DNA conformation
A
B
Conclusions
Curcumin induces cytotoxicity of BCPAP cells.
Induction of apoptosis and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin .
Curcumin inhibited AKT pathway and activated mitogen-activated protein kinase (MAPK) signaling thereby inducing autophagy.
Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei
China Medical University
Guan Haixia
Nangjing University Sun Yang
AcknowledgementsJiangsu Institute of Nuclear Medicine Grants
National Natural Science Foundation of China (No. 30870727)
Natural Science Foundation of Jiangsu Province (No. BK2010156)
Science and Research Foundation of Health Bureau of Jiangsu Province (No. H2008031)