Based on their

mechanism of CO2

fixation

The major reason:- C4 plant have desirable agronomic traits High photosynthetic capacity High water and nitrogen use efficiencies

(leads to rapids growth rates, higher dry matter production and larger economic yields)

Detractive effects of photorespiration

A. Bandyopadhyay, K. Datta, J. Zhang, W. Yang, S. Raychaudhuri, M. Miyao

(2007)

Related journal to the topic

OBJECTIVES Introduce enzymes involved in C4 plant

photosynthesis into C3 plant of rice Determine the effects on photosynthesis

and plant productivity

What is PEPC gene? Phosphoenolpyruvate carboxylase (PEPC)

gene The gene known to play the key role in C4

photosynthesis by catalyzing initial fixation of CO2 in C4 maize

1) TRANSFORMATION BY 1) TRANSFORMATION BY BOMBARDMENTBOMBARDMENT

2) VERIFICATION OF 2) VERIFICATION OF TRANSFORMANTTRANSFORMANT

2) MEASUREMENT OF 2) MEASUREMENT OF PHOTOSYNTHESISPHOTOSYNTHESIS

AND AGRONOMIC DATAAND AGRONOMIC DATA

300 putative transgenic hygomycin resistant plant.

9% of the transformants showed abnormal morphological and physiological.

Genomuc DNA from PCR positive T3 plant showed the presence of non-segregating band of desired sized.

Twenty T1 progenies from the four selected T0 plant were analyzed for the mode of inheritance and segregation of the transgene.

PCR and Southern blot analysis of twenty T1 plant derived from each transgenic line showed the Mendelian segregation of 3:1

Why we need transferring the C4 traits into rice?

Why we use PEPC gene ? Rice is the C3 plant. So, how the C3 can

be C4 plant?

The insertion PEPC gene in indica rice can be done by direct transformation way.

The integration of the gene was confirmed by PCR and Southern analysis.

RT-PCR and Northern blot showed expression of the gene.

The transformant enhancement in photosynthesis rate in high temperature.