controlled rate freezing for cell preservation
DESCRIPTION
The rate of cooling has a dramatic effect on cell viability. Lethal intracellular freezing can be avoided if cooling is slow enough to permit sufficient water to leave the cell during progressive freezing of the extracellular fluid to prevent formation of intracellular ice crystals. That rate differs between cells of differing size and water permeability: a typical cooling rate of about 1°C/minute is appropriate for many mammalian cells after treatment with cryoprotectants such as glycerol or dimethyl sulphoxideTRANSCRIPT
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Custom BioGenic Systems
2101 Controlled Rate Freezer
Margaret Stava [email protected]
562 355-5859 September 23-24, 2013
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Controlled Rate Freezing Overview
Q. What are the most important arguments to use a CRF? A. CRF provides reproducibility, records, and better results.
WHEN THE SAMPLE FREEZES, LATENT HEAT IS RELEASED
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Lethal intracellular freezing can be avoided if cooling is slow enough to permit sufficient water to leave the cell during progressive freezing of the extracellular fluid. A typical cooling rate around 1°C per minute is appropriate for many cells after treatment with cryoprotectants such as glycerol or dimethyl sulphoxide (DMSO), but the rate is not a universal optimum.
Controlled Rate Freezing Overview
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What type of samples do you typically freeze (vials, blood bags, straws)?
Do you freeze more than one size or type of sample?
Is there a size limit to the samples you are freezing?
How many vials / samples will be frozen at any one time?
What temperature ranges are required?
Do you require documentation of the freeze cycle?
Is your facility equipped with liquid nitrogen?
What type of rate freezer are you currently using?
Application Considerations
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Operation
Main menu screen for the selection of all system functions
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The Program Profile Screen is used to program and save your own custom freeze cycle
Operation
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Operation
Q. Can the sample temperature run the freezing program? A. The sample temperature CANNOT operate the freezing program. Sample temperature can only be set as a target temperature switch point.
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Data base screen for input of information regarding each individual freeze run
Operation
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Operation
Q. How much time is needed to warm up the CRF for a next freeze run? A. Approximately 5 minutes.
Q. What is the temperature variation of samples in different locations in the CRF? A. + / - 3 degrees C.
Q. Can CBS advise on other ideal freezing programs? A. CBS can offer suggestions for existing freezing programs.
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Data base search screen for locating saved freeze run information and graphs
Operation
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The freeze cycle profile allows you to view the various temperatures and times of the freeze cycle
Operation
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Run Screen provides all details of actual freeze runs during operation, plus all manual override controls
Operation
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When a liquid turns to solid (freezes), latent heat is released causing a slight rise on the temperature graph.
After the heat is released, the temperature rebounds and holds steady while the samples crystallize .
Once completely frozen, the temperature will fall steadily again according to the programmed rate. When the program is complete the frozen sample can be transferred to long term liquid nitrogen storage.
Heat of Fusion
WHEN THE SAMPLE FREEZES, LATENT HEAT IS RELEASED
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2101 Specifications
System Includes: Laptop controller with 2100 software Freezing Chamber Choice of sample rack 4 Foot LN2 transfer hose Accommodates: 390 4ml / 5ml vials 650 1.2ml / 2ml vials 10-20 bag canisters 130 Canes
All Stainless Steel Construction
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2101 Specifications
Q. What makes the CBS CRF different than others? A. It is 100% programmed and operated by a small computer and software.
All Stainless Steel Construction
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2101 Specifications
Power requirements: 120 volt, 50/60 Hz, 10 amp or 230 volt, 50/60 Hz, 10 amp Interior dimensions: Depth 9.56” (243mm) Height 13.75” (349 mm) Width 14" (356 mm) Exterior dimensions: Depth 25.50" (648 mm) Height 29.25" (743 mm) Including Computer Width 19.06" (484 mm) Weight 76.5 lbs (34.7 kg) System Includes: Laptop controller with 2100 software Freezing Chamber Choice of sample rack 4 Foot LN2 transfer hose
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LN2 Usage
Q. How much LN2 does the CRF use? A. LN2 use will vary with the length of the freezing program. Typical use will be 5 to 7 liters.
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Q. What type of supply tank should be used? A. Any low pressure liquid supply. Best operating pressure = 20 psi / 1.4 bar.
Additional questions
Q. What happens with the CRF and the Laptop during a power failure? A. The system will not operate without a power source.
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Q. How is the temperature probe used?
Additional questions
Q. Is heating also used for thawing? A. Heating is not used for thawing. The heating element is used during some freeze programs and warming the chamber back to room temp
A. The chamber probe actually operates the system. The sample probe provides a trace line on the graph.
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Q. How do you calibrate the probes?
Additional questions
A. Probe calibration is executed through the CRF software.
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Why Custom BioGenic Systems, Inc.
ISO 13485 (medical device) quality system
Family owned and operated for over 25 years
Complete in house engineering and manufacturing
89,000 square foot facility in Bruce Twp., Michigan
In house electronics lab
World-wide sales and service support
Extensive selection of inventory systems
Custom configurations
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State of the art manufacturing
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CBS Freezers are manufactured to ISO 13485:2003 and are certified as MDD 93/43/EEC Class IIA Medical Devices
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Thank you for choosing Custom BioGenic Systems
For more information please contact Margaret Stava
Laboratory/Facilities Equipment Specialist [email protected]
562 355-5859 Fax 1-586-331-2588