conference ayman

7/31/2019 Conference Ayman

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Schistosomiasis is the third most devastatingtropical disease in the world, being a majorsource of morbidity and mortality for developing

countries.

The pathogenesis of schistosomiasis is mainly

due to thehosts

immune response againstSchistosoma eggs trapped in tissues leading togranuloma formation.

In late cases, hepatic fibrosis may lead to end-stage liver failure.


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Although many hopes rely upon livertransplantation as a treatment, thereare limited available donor livers for

the hundred millions of patients

worldwide.

Thus, it is very important toinvestigate new therapies.


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MSCs show great plasticity &can differentiate into differentbody cells includinghepatocytes. MSCs can replace

diseased or dysfunctioningcells with healthy functioningones.

For these reasons, the MSCsare currently being tested fortheir potential use in celltransplantation therapy for a

number of diseases.

However, this concept needs to beextensively investigated beforeclinical trials on human cases.


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Murine schistosomiasis model of hepatic

fibrosis represents an experimental modelof liver collagen deposition, the course ofwhich resembles that of the humandisease.

Thus, trials on the impact of MSCs therapyon murine model are very essential from

both the scientific and ethical points ofview before clinical trials on human cases.


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1- Infection of Swiss albino female mice withSchistosomamansoni

2- Separation of BM-MSCs from male mice andcell culture:

(x200)


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3- MSCs administration into the liver(in situ) of the test group at 10 weeks

post infection (500,000 cells/ mouse)

4- Mice scarification at 4 weeks post

administration

5- Histopathological examination &

morphometric analysis of liver slidesstained with H&E or Masson trichrome(to assess fibrosis)


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7- Electron microscopy of liver slides to

detect newly formed hepatocytes

6- Immunno-histochemical examinationof liver sections to detect -SMA

8- PCR detection of male-derived MSCs

9- Statistical analysis


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Histopathological examination &morphmetric analysis of liver slides stained

with H&E

H&E (x100)


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Histopathological examination & morphometricanalysis of Masson trichrome stained liver slides

(to assess fibrosis)

MT (x100)


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Immunno-histochemical examination ofliver sections to detect -SMA

x100


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Electron microscopy to detect newlyformed hepatocytes

x4000


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PCR detection of male-derived MSCs


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BM-MSCs represent a promising toolfor the remodelling of hepatic

granulomas & fibrosis by regeneration ofhepatic tissue & decrease fibrosis incases of hepatic injury induced by

Schistosoma mansoniinfection in mice. Further studies about theimmunological mechanisms that

backgrounds the BM-MSCs remodellingrole in hepatic granulomas & fibrosis arerecommended.


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Extensive studies in vitro as well invivo are needed to achieve a betterunderstanding of the potential benefits

and risks of MSCs (either autologous orallogenic) and their therapeutic use inclinical cases of schistosomal hepaticinjury.

Administration of MSCs at differentintervals post infection could be studied

to determine the most appropriate timingof MSCs administration.


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