COLD SPRING HARBOR SYMPOSIA

ON QUANTITATIVE BIOLOGY

VOLUME XXXV

TRANSCRIPTION OF

GENETIC MATERIAL

UNIVERS8TATSBBLIOTHEKHANNOVER

TECHNISCHEINFORMATIONSBIBUOTHEK

~J

COLD SPRING HARBOR LABORATORY

COLD SPRING HARBOR, L.I., NEW YORK

1970

Contents

Foreword v

List of Previous Volumes vi

Photographs of Some Symposium Participants viii

List of Those Attending The Symposium xi

RNA SEQUENCES

Cory, S., P. P. Spahr, and J. M. Adams: Untranslated Nucleotide Sequences in

R17 Bacteriophage RNA 1

Jeppesen, P. G. N., J. L. Nichols, F. Sanger, and B. G. Barbell: Nucleotide

Sequences from Bacteriophage R17 RNA 13

Smith, J. D,, K. Anderson, A. Cashmore, M. L. Hooper, and R. L. Russell:

Studies on the Structure and Synthesis of Escherichia coli Tyrosine Transfer

RNA 21

Fellner, P,, C. Ehresmann, and J. P. Ebel: Nucleotide Sequence Analysis of

Sections of the 16 S RNA from E. coli 29

Larsen, C. J,, P. Lebowitz, S. M. Weissman, and B. DuBuy: Studies of the

Primary Structure of Low Molecular Weight Ribonucleic Acid other than

tRNA 35

BACTERIAL RNA POLYMERASE—I

Zillig, W., K. Zeohel, D. Rabussay, M. Sohachner, V. S. Sethi, P. Palm, A.

Heil, and W. Seijtert: On the Role of Different Subunits of DNA-De-

pendent RNA Polymerase from E. coli in the Transcription Process 47

Cassani, G., R. R. Burgess, and H. M. Goodman: Streptolydigin Inhibition of

RNA Polymerase 69

Hinkle, D. C. and M. Chamberlin: The Role of Sigma Subunit in TemplateSite Soleotion by E. coli RNA Polymerase 65

Krakow, J. S, and K. von der Helm: Azotobact&r RNA Polymerase Tran¬

sitions and the Rolease of Sigma 73

Jacobson, A. and D. Gillespie : An RNA Polymerase Mutant Defective in ATP

Initiations 85

Davison, J., K. Brookman, L. Pilarski, and H. Eohols: The Stimulation of

RNA Synthesis by M Factor 95

BACTERIAL RNA POLYMERASE—II

Goifir, 0. G. and K. Weber: A T4-Induced RNA Polymerase a Subunit

Modification 101

Bremer, H.: Influence of KCL on the in vitro Transcription of T4 DNA 109

Roberts, J. W.; Tho p Factor: Termination and Anti-termination in Lambda 121

Richardson, J. P.: Rho Factor Function in T4 Transcription 127

Millette, II. L., C. D. Trotter, P. Herrlioh, and M. Sohweiger: In vitro

Synthesis, Termination, and Release of Active Messenger RNA 135

Maitra, U., A. H. Lookwood, J. S. Dubnoef, and A, Guiia: Termination,

Roloaso, and Reinitiation of RNA Chains from DNA Templatos by Es¬

cherichia coli RNA Polymerase 143

Goldberg, A. R.: Termination of in vitro RNA Synthosis by p Factor 157

xix

xxCONTENTS

PHAGE TRANSCRIPTION—1

Sedat, J. W. and R. L. Sinsheimer: The in vivo </>X mRNA 1(13

Hayasiii, Y., and M. Hayashi: Fractionation of <I>X174 Specific Messenger

RNA 171

Takanami, M., T. Oicamoto, and M. Sugiura: The Starting Nucleotide Sequen¬

ces and Size of RNA Transcribed in vitro on Phage DNA Templates 17!)

Young, E. T.: Control of Functional T4 Messenger Synthesis 18!)

Jayaraman, R. and E. B. Goldberg: Transcription of Bacteriophage T4

Genome in vivo1 !>7

Brody, E., R. Sederopf, A. Bolle, andR. H. Epstein: Early Transcriptionin T4-Infccted Cells 203

PHAGE TRANSCRIPTION—11

Casoino, A., S. Riva, and E. P. Geidusohek: DNA Ligation and the Coupling

of T4 Late Transcription to Replication 213

Grau, O., B. M. Ohlsson-Wilhelm, and E. P. Gioiduhciiek: Transcription

Specificity in Bacteriophage SPOl Development 221

Bautz, E. K. F. and F. A. Bautz: Studies on the Function of the RNA Poly¬

merase a Factor in Promoter Solection 227

Haqer, G., B. D. Hall, and K. L. Fields: Transcription Factors from 're¬

infected Escherichia coli 233

Travers, A.: RNA Polymerase and T4 Development 241

Summers, W. C. and R. B. Siegel: Regulation of Coliphage T7 RNA Metab-

olism in vivo and in vitro 2,r>3

Chamberlin, M. and J. MoGrath: Characterization of a T7-Specilie RNA

Polymerase Isolated from E. coli Infected with T7 Phage 25!)

Linial, M. and M, H. Malamy: The Effect of V Faoloi'H on RNA Synthesis and

Degradation after Infection of E. coli with Phage cf>\ I 2153

Davis, R. W. and R. W. Hyman: Physical Locations of the in vitro RNA Initia¬

tion Site and Termination Sites of T7M DNA 2150

PHAGE TRANSOM PT1 ON—111

Ciiadwiok, P., V. Pirrotta, R. Steinherg, N. Hopkins, and M. Ptakhnk: The

X and 434 Phage Repressors 2K3

Green, M. H., W. S. Hayward, and P. Gariglio: A Method for the Locali¬

zation of Active Promoters 2!»5

Kourilsky, P., M. F. Bourguignon, M. Bouquet, and F. Groh: Early Tran¬

scription Controls after Induction of Prophage A 305

Hahnemann, S. V. and W. G. Spiegelman; Role of the Gone N Product in

Phage Lambda 315

Champoux, J. J.: The Sequonco and Oriontation of Transcription in Bacterio¬

phage X 31!)

Spiegtslman, W. G., S. F. Heinemann, P. Bracket, L. Perkira da Silva, and

H. Eisbn: Regulation of tho Synthesis of Phage Lambda Repre+tHor 325

Kumar, S., E. Galef, and W. Szyhalhki: Regulation of the Tranxeripfinn of

Escherichia coli Phage X by its Early Couch N and iof 331

Szybalski, W., K. Bovre, M. FiANivr, K. Hayes. Z. IIkadkcna, K. Kcmak,

11. A. Lozeron, H. J. .1. Ni.JKamp, and W. K. Ktkvionk: TranscriptionalUnits and Their Controls in Escherichia coli Phage X: Operons and

Scriptons ,, ml

Hkrskowitz, 1. and E. Signkh: Control of Transcription from the r Strand of

Bacteriophage Lambda ;ift,ri

KciiLisiK, R. and J. Urkknhlatt: Transcription in the Lambda-a/*/ Phage ... 3(>!l

Naono, S. and K. Tokpyama: On the Mechanism of / DNA TnuiMTiptiotiin vitro ;!75

CONTENTS xxi

RELAXED VERSUS STRINGENT CONTROL

Lazzarini, R. A. and R. M. Winslow: The Regulation of RNA Synthesis duringGrowth Rato Transitions and Amino Acid Deprivation in E. coli 383

Primakoff, P. and P. Berg: Stringent Control of Transcription of Phage^80ps?i3 391

Gallant, J., H. Erlich, B. Hall, and T. Laffler: Analysis of the RC Func¬tion 397

Casiiel, M.: Inhibition of RNA Polymerase by ppGpp, a Nucleotide Accumu¬lated during the Stringent Response to Amino Acid Starvation in E. coli 407

Travers, A,, R. Kamen, and M. Casheiv The in vitro Synthesis of RibosomalRNA 415

POSITIVE AND NEGATIVE METHODS FOR CONTROL OF

TRANSCRIPTION

Perlman, R., B. Chen, B. deCrombrugghe, M. Emmer, M. Gottesman, H.

Varmus, and I. Pastan: The Regulation of lac Operon Transcription byCyclic Adenosine 3',5'-Monophosphate 419

Ohshima, Y., T. Horiuchi, Y. Iida, and T. Kameyama: Transcription and

Repression of the lac Operon in vitro 425

Zubay, G., D. Schwartz, and J. Beokwith: The Mechanism of Activation ofCatabolite-Sensitive Genes '. 433

Arditti, R., L. Eron, G. Zubay, G. Toooiiini-Valentini, S. Connaway, andJ. Beokwith: In vitro Transcription of the lac Operon Genes 437

Losick, R., A. L. Sonenshein, R. G. Shorenstein, and C. Hussey: Role of

RNA Polymerase in Sporulation 443

KINETICS OF RNA SYNTHESIS AND DEGRADATION

Adesnik, M. and C. Levintiial: The Synthesis and Degradation of Lactose

Operon Messenger RNA in E, coli 451

Mosteller, R. D., J. 11. Rose, and C. Yanofsky:Transcription Initiation and

Degradation of trp mRNA 461

Baker, R. and C. Yanofsky: Transcription Initiation Frequency for the

Tryptophan Operon of Escherichia coli 467

Imamoto, F., Y. Kano, and S. Tani: Transcription of the Tryptophan Operonin Nonsense Mutants of Escherichia coli 471

Lavalle, R. and G. de Hatjwer: Repression by Tryptophan at the Level of

Transcription and Translation in E. coli 491

Morse, D. E.: "Delayed-Early" mRNA for the Tryptophan Operon? An Effect

of Chloramphenicol 495

Pato, M. L. and K. von Meyenburg: Residual RNA Synthesis in Escherichia

coli after Inhibition of Initiation of Transcription by Rifampicin 497

EUCARYOTIC RNA

Miller, 0. L., Jr., B. R. Beatty, B. A. Hamkalo, and C. A. Thomas, Jr.:

Electron Microscopic Visualization of Transcription 505

Daneiiolt, B., J.-E. Edstrom, E. EgyhXzi, B. Lambert, and U. Ringborg:

RNA Synthosis in a Balbiani Ring in Ohironomus tentans 513

Pelling, C: Puff RNA in Polytene Chromosomes 521

Ashburner, M.: A Prodromus to the Genetic Analysis of Puffing in Drosophila 533

SoHEiuiER, K. and G. Spohr: Nucloar and Cytoplasmic Messenger-Like RNAand Thoir Relation to the Active Messenger RNA in Polyribosomes ofHeLa

Cells 539

Darnell, J. E., G. N. Pagoulatos, U. Lindberg, and R. Balint: Studies on

the Relationship of mRNA to Heterogeneous Nuclear RNA in Mammalian

Colls

xxii CONTENTS

Penman, S., H. Fan, S. Perlman, M. Rosbash, R. Weinberg, and E. Zylber:

Distinct RNA Synthesis Systems of the HeLa Cell 5(51

Perry, R. P., J. R. Greenberg, and K. D. Tartof: Transcription of Ribosomal,

Heterogeneous Nuclear, and Messenger RNA in Fucaryofes 577

Grierson, D., M. E. Rogers, M. L. Sartirana, and V. 10. Lokning: The

Synthesis of Ribosomal RNA in Different Organisms: Structure and

Evolution of the rRNA Precursor 58!)

Attardi, G., Y. Aloni, B. Attardi, D. Ojala, L. Pica-Mattocoia, D. L.

Robrerson, and B. Stobrie: Transcription of Mitochondrial DNA in

HeLa Colls 5!)!)

McCarthy, B. J. and J. D. Duerkren : Fractionation of Mammaliun ('hromatin (521

Chetsanga, C. J., D. L. Poocia, R. J. Hill, and P. Doty: Stago-Speeilie RNA

Transcription in Developing Sea Urchins and Their Chromatins (12!)

Tomkinh, G. M., D. W. Martin, Jr., R. H. Stellwagen, J. I). Baxter,

P. Mamont, and B. B. Ljovinhon: Regulation of Specific Protein SynthcHisin Eucaryotic Cells (535

THE RNA POLYMERASE OF MAMMALIAN CULLS

Furtii, J. J. and G. E. Austin: RNA Polymerase of Lymphoid Tissue: A

Preliminary Characterization of the Enzyme and tho RNA It Synthesizes (541

Blatti, S. P., C. J. Ingles, T. J. Lindell, P, W. Moiuua, R. F. Weavuu, F.

Weinberg, and W. J. Rutteu: Structure and Regulatory Properties of

Eucaryotic RNA Polymerase (54!)

Monjaiidino, J. P. and L. V. Crawford : RNA Polymerase from Mouse FmbryoColls '.. (55!)

Sugden, B. and J. Sambrook: RNA Polymerase from HeLa Cells (1(53

Keller, W. and R. Goor: Mammalian RNA Polymerase: Structural and

Functional Properties (571

Jacob, S. T,, E. M. Sajdel, W. Mueokb, and II. N. Munuo: Soluble RNA

Polymerases of Rat Liver Nuclei: Pro])orties, Template, Speeilicity, and

Amanitin Responses in vitro and in vivo (IK1

ClIAMBON, P., F. GlHSINGER, J. L. MANUEL, JR., C. REDING, M. GnIAZDOW-

ski, and M. Meihlao: Purification and Properties of Calf Thymus DNA-

Dopeiuk>nt RNA Polymerases A and B (i!>3

Stein, H. and P. IIausmn: Factors Influencing flic Activity of Mammalian RNA

Polmyeraso 70!)

Seifart, K, H.: A Factor Stimulating the Transcription on Double-Stranded

DNA by Purified RNA Polymerase from Rat Liver Nuclei 71!)

Roeder, R. G., R. H. Reeder, and D. I). Brown: Multiple Forms of RNA

Polymerase in Xcnopm laevis: Their Relationship to RNA Synthesis in

vivo and Their Fidelity of Transcription in vitro 727

Tqcchini-Valentini, G. P. and M. Crippa: RNA Polymerases from Xcnopuxlaevis 737

ANIMAL VIRUS GENOMICS

Kates, J.: Transcription of the Vaccinia Virus Genome and the Occurrence of

Polyriboadcnylic Acid Sequences in Messenger RNA 743

Roizman, I?., S. Baoheniietmer, E. K. Wagner, and T. Savage: Synthesis and

Transport of RNA in llerpcHviriiH-lnfootcd Mammalian Cells 753

Miliavari), S. and M.Nonoyam a: Segmented Structure of the Reovirus Genuine 773

Shatkin, A. J. and A. K, Banerjbk: In vitro Transcription of Double-Stranded

RNA by Reovirus-Associated RNA Polymerase 7K1

Joklik, W. K., J. J. Skehkl, and II. J. Zwheiunk: The Transeriittion of the

ReoviniM Gonomo 7!) 1

CONTENTS xxiii

Green, M., J. T. Parsons, M. Pina, K. Fujinaga, H. Caffier, and I. Land-

graf-Leurs: Transcription of Adenovirus Genes in Productively Infected

and in Transformed Cells 803

Westhial, H. and E. D. Kiehn: The in vitro Product of SV40 DNA Tran¬

scription and Its Specific Hybridization with DNA of SV40-Transformed

Cells'

819

Tonegawa, S., G. Walter, A. Bebnardini, and R. Dulbecco:' Transcriptionof the SV40 Genome in Transformed Cells and during Lytic Infection 823

Martin, M. A.: Characteristics of SV40 DNA Transcription during LyticInfection, Abortive Infection, and in Transformed Mouse Cells 833

Baltimore, D.: RNA-Dependent Synthesis of DNA by Virions of Mouse

Leukemia Virus 843

Mizutani, S. and H. M. Temtn: An RNA-Dependent DNA Polymerase in

Virions of Rous Sarcoma Virus 847

Chamberlin, M. J.: Transcription 1970: A Summary 851