R & D Activities of DYMT/PYPT, Haridwar

Avnish K. UpadhyayBAMS, PGDCR, ROTP, Ph.D.(Pursuing)

Senior Scientist & H.O.D.Department of Ayurved Research & Development

Department of Ayurved Research & Development

The primary roadblocks to the credibility and global success of the Ayurveda are lack of product standardization, quality control and absence of documental evidence for clinical efficacy. Success in clinical development is key to the long-term success of Ayurveda or any other system of Medicine.

Department will do extensive research in new traditional drug development and their safety and efficacy trials on animals and human subjects.

Aims:

To enhance the capability of the DYM(T) & PYP(T) as a premier institution for research in Ayurveda and forge strategic alliance with similar establishments and constantly strive for excellence in basic and applied knowledge for efficient understanding of the cause and prevention of human diseases and their management

Objectives:

Formulate aims and patterns of research on scientific lines in Ayurvedic systems of medicine.

Initiate, develop, encourage and coordinate scientific research in fundamental and applied aspects of Ayurveda.

To study of diseases, their prevention and cure, especially with emphasis for covering the rural population of the country and the rest world.

Propagate basic knowledge and experimental measures relating to the cause and prevention of diseases and exchange information with other institutions with similar approach.

To Fulfill the imagination of HH Swami Ramdev ji & HH Acharya Balkrishna ji to make disease free India & World by reestablishing traditional Indian knowledge.

Areas of Research:

Basic Research Clinical Research Drug Research Literary Research New Drug Development Collaborative Research Studies

Clinical Research:

Clinical Studies Survey & Surveillance Program Community Health Care Program Tribal Health Care Program

Drug Research:

Medico-Botanical Survey Cultivation of Medicinal plants Pharmacognostical Research Program Plant Tissue Culture Chemical Research Program Pharmacological Research Program including

Toxicological studies

Drug Standardisation Research Program

Literary Research:

Publication of Rare and Classical manuscripts of Ayurveda.

Publication of scientific Journal/Bulletin Publication of Activities in the form of Newsletter Publication of Pamphlets on Research findings Development of Video-films on various research

achievements

Details of Major R & D Projects/Studies Completed:

1. Botanical Identity of Astavarga Plants: The medicinal plants identified as Astavarg are viz. Jeevak-  Malaxis acuminata D. Don;  Rishbhak-   Malaxis muscifera (Lind.) Kuntze; Meda- Polygonatum verticillatum Allioni, Mahameda- Polygonatm cirrifolium Royle; Ridhi- Habenaria intermedia D. Don; Vridhi- Habenaria acuminata Lindl; Kakoli- Roscoea alpina Royle and Kshirkakoli- Lilium polyphyllum D. Don The hilly and remote forest areas have been surveyed for specific programme are viz. Auli, Joshimath, Tapovan, Badrinarayan, Valley of Flowers, Hemkund, Ukhimath, Gangotri Valley, Kedarnath, Renthal, Gangnani, Yamunotri Valley, Rishikesh, Dhanaulti in Uttarakhand and Karsog, Jalpa, Kamarunag, Rewalsar, Rewanda, Lahaul-spiti, Hatloo, Thanedhar, Narkanda, Matiana, Shilaru in Himachal Pradesh. 

2. Collection of Medicinal Plants:

There are a large number of medicinal plant diversity widely used in ayurveda are present in the Garden. The live plants are also prescribed by the Vaidyas of Patanjali Yogpeeth to patients for taking fresh extract and decoction according to their dose of administration. Some common species are viz. Abroma augusta (Ulta Kambal); Acorus calamus (Vacha);  Aloe vera (Ghee kwar); Andrographis paniculata (Kalmegh); Argyreia speciosa (Vidhara); Asparagus adscendens, A. racemosus (Shatavar);  Bauhinia variegata (Kachnar);  Berberis aristata (Daruhaldi);  Boerhavia diffusa (Punarnava);  Bryophyllum pinnata (Pashanbhed/Patharchur); Clerodendrum phlomidis (Bharangi bhed); C. serratumn (Bharangi); Cassia occidentalis (Kasmarda); C.  tora (Chakramard);  Centella asiatica (Mandook parni);  Cinnamomum camphora (Kapoor);  Clitorea ternatea (Aprajita); Cocculus villosus (Garudi, Patalgarudi);  Crinum latifolium (Sudarshana); Curculigo orchioides (Kali musali);  Desmodium gangeticum (Salparni); Erythrina indica (Paribhadra);  Ficus racemosa (Gular);  Gendarussa vulgaris (Kala bansa);  Gmelina arborea (Gambhari);  Mimosa pudica (Lajavanti);  Ocimum americanum. (Rama tulsi),  Oroxylum indicum (Shyonak); Paederia foetida (Gandha prasarani);  Phyllanthus fraternus (Bhumi Amla);  Piper longum (Pippali);  Plumbago capensis, Plumbago zeylanica (Chitraka);  Rauvolfia sepentina (Sarpagandha);  Ricinus communis (Eranda);  Sida cordifolia (Bala);  Solanum indicum (Brhati);  S. surratense (Chhoti Kateli, Kantkari); Terminalia arjuna (Arjun); T. bellirica (Bahera);  T. chebula (Harre);  Tinospora cordifolia (Giloy);  Tylophora indica (Dama buti);  Uraria picta (Prisniparni); Viola odorata (Banafsa); Vitex negundo (Nirgundi);  Withania somnifera (Ashwagandha) etc.

3. Field Experiments of Organic Farming Technology by the use of   Wormi Compost and NADEP Manure:

In nature there is a system of maintaining biological balance in soil, air and water. The human intervention has damaged this balance due to short-run gains. This is a great threat towards the sustainability of agricultural production to meet the food and nutritional requirements of growing population. Over the past four decades, the use of chemical fertilizers, pesticides, herbicides, weedicides, excessive soil erosion etc. have adversely affected the soil fertility, productivity and quality of produce. Growing awareness of people about environmental degradation, has led the scientists, policy planners and executives to take corrective steps in order to improve the quality farming. The department has developed Divya wormi compost and Divya NADEP to the farmers for scientific and safe cultivations.

4. Effect of Yoga on Physical Fitness, Work Efficiency and Psychological State in Army Recruits

Both yoga and physical training groups showed comparable improvement in leg and back strength, though the yoga group showed better spinal flexibility. The yoga group showed an increase in lean muscle mass while the physical training group showed an increase in body fat. This re-distribution in body composition is of great importance to this group. The yoga group showed no decrease in level of arousal (or alertness) but showed a consistent decrease in blood pressure suggesting maintained arousal was not associated with mental stress. Hence yoga was a state of ‘alertful rest’, especially important in this group. The physical training group showed a decrease in arousal/alertness. The yoga group showed an improvement in skilled activities requiring co-ordination, concentration, as well as muscular strength and endurance. This would be especially useful for activities such as shooting at a target.

5. Clinical evaluation of beneficial effects of yoga and pranayama in residential yoga science camps

In order to establish Yoga as a science, especially medical science we need the contribution of a sage, who is not only an accomplished yogi but also understands the process of testing and proving the concepts of Yoga using modern scientific methods and standards. One such person is Swami Ramdevji who has studied modern medical science in depth along with Yoga and has proved that Yoga practices meet modern medical standards with courage and confidence he has invited international scientists to work together and with him to evaluate and prove the scientific basis of Yoga. Selecting a few asanas from the vast repertoire of Yoga, which could be used by all for preventing diseases, and controlling recurrence of diseases, and at the same time providing benefits like awakening talents, generating positive, creative, qualitative and productive qualities, etc.

6. Effect of yoga and pranayama on bone mineral density- An open level clinical study

Dot Hemorrhage

The data of bone mineral density test collected before and after Yoga practice was analyzed statistically. The results were drawn with the theory of sign of nope-parametric test and efficiency level and confidence level of patients is being presented here in tabular form.

The efficiency and confidence level in (men) osteoporosis patients was 4.24 and 99.73 per cent respectively. The figures in case of women of the same disease were 4.017 and 99.73 per cent respectively. In the same way patients of osteopenia (men) showed efficiency and confidence level of 4.67 and 99.73 per cent, whereas the women showed of 3.66 and 99.73 per cent, respectively.

This research makes it clear that the T-score of bone mineral density shows considerable improvement along with increasing the strength of bone after 40 days of Yoga practice.

7. Immediate Effect of a Yoga Practice Session on State Anxiety in Scientists from Different Asian Countries

Hard Exudates The present study intended: (i) to assess the state anxiety

immediately before and after a yoga session in scientists who were attending an international seminar on an unrelated subject (i.e., in hydrology at IIT Roorkee), and (ii) to compare the results in scientists from different Asian countries, such as India, Nepal, Bangladesh, Pakistan, and China There was a group significant decrease in state anxiety (p<.001, paired t-test; n = 22). This has been shown as a graph below. Also irrespective of the country from which they came, the scientists showed a decrease in state anxiety after yoga.

8. Psychosomatic effects of yoga - a pioneering study based on large scale survey

The present survey clearly indicates that Yoga rejuvenates the personality, it acts like life-saving medicine, helps in overall development, guides on the path of accomplishment, fulfilling duties, achieving economic progress, fulfilling the responsibilities of household life and gaining liberation. Yoga is a holistic science and fully capable of giving desired results. It improves physical health, mental development, intellectual power, internal progress, and spiritual strength. It is not just an expression, but also an experience of life, as shared by thousands of participants. The scientists and medical practitioners are taken aback with the miraculous results of Yoga and Pranayama.

9. A novel method and scheme for Standardization of Divya Madhunasni: A Herbal Medicine

The Divya Madhunasini is a polyherbal drug constitute of a complex mixture of different herbal & mineral substances. We have undertaken a task to develop a novel scheme for sensitive, specific & accurate standardization the Divya Madhunasini.The present method will provide the sensitive, specific & accurate scheme for standardization of other polyherbal drugs for their consistency, potency and efficacy in future. Packet of 120 tablets of Madhunasini 500 mg has been taken the batch No.DPO-12 Exp. 9/2009. The Madhunasini is a mixture of 23 Poly herbal Material. We have categorized all 23 herbomineral material into the four classes categorizes. i.e. alkaloids contents, glycosides contents, bitter contents & tannins contents. The each categorizes extract of Madhunasini exhibits the characteristic spot at differed Rfs. The result indicates that for the Standardization of Polyherbal preparation the extraction of complex mixture of drug with various solvents is necessary for the detection, characterization & identification of active chemical and therapeutic ingredients. Whereas material extracted with one solvent was not exhibit any clear Rf values and always appear in the form of tail.

10. In-vivo toxicological study of Drishti eye drop

0.1 Ml of the Drishti eye drop was administered as such to the eyes and its associated mucus membranes in the albino rabbits thrice in a day (Dose: 0.3 ml).The animals were examined after 1, 24, 48 and 72 hours and thereafter daily for 21 days for any irritation in eye on following criteria’s Retinal Reflex, Papillary Reflex, Adnexa –Lids, Iris, Aqueous humour, Lens, Vitreous humour, Retina, Optic Disc, Tapedum Lucidum, Tapedum Nigrum. The results of the present study suggest that Drishti eye drop is “Non Irritant” to the eye and its associated mucus membranes at the dose of 0.3 ml. The present study is obtained information on health hazards likely to arise from a short term exposure .The study has been conducted as per “Drug and Cosmetics Rules 2005 Ref OECD GUDLINES No. 405” for non clinical laboratory studies.

11. In-vivo toxicological study on plant Ricinus Communis Linn found non toxic after the detoxification done by ayurvedic methods

Ricinus Communis Linn extract is safe and could be used therapeutically .The extract of the plant were given intervening with adjuvant for 1, 2, 7,8,14 & 30 days in equal doses. We found the best response at the dose of 0.1 mg /100gm of body weight in rabbits.

Our result shows that controlled amount of detoxified seeds extract of Ricinus Communis Linn exhibits no significant effect in the level of Total Serum proteins, SGOT and Alkaline Phosphate in the rabbit model. Our result suggests that this plant is not merely a source of laxative oil; its active constituents exhibit the highly potential anticancer property without toxic effects.

12. Efficacy evaluation of Drishti Eye drop (A product of Patanjali Ayurved Limited) in different eye disorders.

This prelimnary clinical study was conducted to evaluate the effect of Drishti Eye Drops, a poly herbal preparation in 53 subjects with various types of eye disorders at the Department of Ophthalmology and Research, Divya Yog Mandir Trust, Patanjali yog Peeth, Haridwar. At the onset of treatment i.e. screening visit baseline assessment was taken following General examination of eye, Examination of visual acuity and subjective relief of symptoms related to refractive error, cataract, Glaucoma etc. The patients were instructed to instil 2 drops of Drishti Eye Drops in each eye 3 times a day for 15 days. At the end of the therapy period, it was found that the drug had considerably reduced symptoms related to refractive error, cataract, Glaucoma etc, increase in visual acuity, inflammatory conditions of eye and improvement in general eye conditions.

13. Psychophysiological effects of an eight-day yoga training on healthy volunteers and those with metabolic syndrome conducted by divya yog mandir trust in collaboration with sahara india medical institute, lucknow, SGPGI lucknow & Swami viveaknanda yoga anusandhan samsthan (SVYASA), bangalore.

14. Effect of Pranamaya on stress hormones: a double blind randomized controlled trial conducted by divya yog mandir trust in collaboration with sahara india medical institute, lucknow, SGPGI lucknow & Swami viveaknanda yoga anusandhan samsthan (SVYASA), Bangalore

15. Effect of pranayama and yoga on lipid profile in normal healthy athletes (Footballers) conducted by divya yog mandir trust in collaboration with Mohun Bagan Athletic Club, Kolkata.

Details of Major R & D Projects/Studies in Progress:

1. Collaboration in Research with National Bureau of Plant Genetic Resources (NBPGR) and Research on Astavarg plants:

Few years back Swami Ramdevji visited NBPGR, Head Quater at New Delhi and later research collaboration has been made on Plant Biodiversity to survey, identify and collect Ashtavarga plants used as ingredients in Chayavanprash. This project provided a good opportunity to undertake exploration and collection tours in Hilly areas of Uttrakhand and Himachal Pradesh and about 120 medicinal and aromatic plants were collected and planted in the Divya Vanaushadhi Vatika.

2. Effect of a Polyherbal Ayurvedic preparation in Patients with Non-Insulin Dependent Diabetes Mellitus (NIDDM): A randomized controlled Trial

Many ayurvedic formulations and herbs given in traditional texts used by ayurvedic practitioners for long times like Trigonelle foenum-graecum, Syzigium cumini, Gymnema sylvestre etc. are looking very much useful in different types of diabetes. The assessment of relative importance of Polyherbal drug in NIDDM patients especially pathological and symptomatic changes will be done in this study. The result of this study will decide long term study on different type of Diabetes patients. This is a randomized prospective, single centre single blind study design. Sixty eight newly diagnosed NIDDM patients will be recruited and randomized in a 1 (Wait-List Control group- Placebo): 1 Polyherbal Preparation (ayurvedic regime) fashion and followed for at least two years.

3. Effects of Mukta Vati (A Nobel Herbomineral Preparation of Divya Pharmacy) in Patients With High-Normal to Stage I Hypertension : A randomized controlled comparative study

Hypertension and diabetes mellitus are prevalent disorders of middle class population. Hypertension is the major silent killer of mankind. The herbal formulation used in this project certainly helps to control and cure the hypertension. There are many drugs in different pathies but it is quite effective and less toxic in comparison to other drug. The entire work of this project is designed in such way that it will cover all the aspects of the process i.e., chemical, botanical, microbiological, pharmacological screening as well as clinical trial. Special attention will be given on the evaluation of modern technique i.e., quality and quantity assurance at each step of the process

4. Project Sponsored by National Medicinal Plant Board:

A project entitled, “To standardize agro-techniques for Gloriosa superba, Withania somnifera, Rauvolfia serpentina and Andrographis paniculata.”  for three years has been sanctioned by the Board. 

5. A Randomized, Double-Blind, Controlled Study of a Herbomineral Preparation of Divya Pharmacy in adult Patients of mild to moderate Bronchial Asthma

A Herbomineral combination of Divya Pharmacy found very much effective in Asthama patients in General Practice containing Glycyrhiza glabra, Justicia gendarussa, Solanum surattenes, Adhatoda vasica, Ocimum sanctum, Cinnamomum zeylanicum, Zingiber officinale, Abhrak Bhasma, Swarn Basant Malti, Sanjivani Vati etc.To treat this widespread disease there is a high prevalence of usage of herbal medicine. The use of plants is as old as humankind and it has been steadily increasing over the past 10 years.

6. Effect of An Ayurvedic Herbomineral preparation by Divya Pharmacy in the treatment of AIDS (Ojakshaya): A randomized controlled comparative study

Many ayurvedic formulations given in traditional texts used by ayurvedic practitioners for long times like Heerak Bhasma, Ras Manikya, Swarn Basant Malti, Amrita sat etc. are looking very much useful in different types of immune related disorders, may be due to lekhan properties of these drugs.

7. Effect of A Herbomineral Preparation by Divya Pharmacy in Women with Non-Metastatic Breast Cancer: A randomized controlled comparative study

Many ayurvedic formulations and Herbs given in traditional texts used by ayurvedic practitioners for long times like Heerak Bhasma, Ras Manikya, Swarn Basant Malti, Tal Sindur, Amrita sat etc. are looking very much useful in different types of carcinomas, may be due to lekhan properties of these drugs.

8. To study the effect of Swami Ramdev yoga (SRY) sequences on Human gene expression- a randomized control clinical trial.

9. To study the peak plasma concentration of metals after the consumption of bhasma.

10. Open prospective clinical trial and study of shilajit as potential anti aging agent that maintained telomere length in cells by increasing the activity of telomerase, the enzyme involved in telomere synthesis.

11. To study the effect of Swami Ramdev yoga (SRY) sequences on the activity of peroxidase in mitochondria increases with age in healthy mammals, in order to counter the increased oxidative stress associated with ageing.

Research Lab(s):

1. QA/QC Lab, Divya Pharmacy, A-1 Industrial Area, Haridwar- 249401, Uttarakhand, India, Ph: 01334-265857, 266583 Telefax: 01334- 265859 Email: divyapharmacya_1@rediffmail.com 2. Diagnostics and Research Lab, Patanjali Yoga Peeth, Maharshi Dayananda Gram, Delhi- Haridwar National Highway, Haridwar, Uttarakhand, India Phone No: 01334-240008, 246737,244107 Fax: 01334-244805, 240664Email: divyayoga@rediffmail.com3. R & D Lab, D-38 Industrial Area, Haridwar-249401, Uttarakhand, IndiaPhone No: 01334- 265370 Email: patanjaliayurveda@rediffmail.com 4. Patanjali Herbs & Agro Research Lab, Divya Nursery, Delhi- Haridwar National Highway, Haridwar-249408, Uttarakhand, India Email: pyp_agrotechresearch@rediffmail.com 5. Yog Research Lab Patanjali Yoga Peeth, Maharshi Dayananda Gram, Delhi- Haridwar National Highway, Haridwar, Uttarakhand, India Phone No: 01334-240008, 246737,244107 Fax: 01334-244805, 240664Email: divyayoga@rediffmail.com

Major Equipments:

S.No. Name of The Equipment Company

1. Coupled Plasma-Optical Emission Spectrometers . (ICP-OES)

Varian

2. WinCats, HPTLC system CAMAG

3. HPLC/GC/Todac Konictech

4. XL-600, Fully automated biochemistry analyzer Transasia Bio-Medicals Ltd., Mumbai

5. XL-300, Fully automated biochemistry analyzer Transasia Bio-Medicals Ltd.

6. Sysmex 2000i, Fully automated hematology count. Transasia Bio-Medicals Ltd.

7. TRITURUS, Fully automated ELISA processor GRIFOLS USA

8. BioRad D-10, HbA1c & Thalassaemia analyzer Bio-Rad Laboratories, Inc.

9. Turbox-Plus, Immuno-protein assays Orion Diagnostica

10. Fully automated Blood Gas analyzer Vedica

11. Fully automated random chemistry analyzer Transasia Bio-Medicals Ltd.

12. Fully automated Na analyzer Transasia Bio-Medicals Ltd.

13. CASE, cardiac assessment system for exercise testing GE Healthcare

14. MAC, 1200 ST, Resting/Stress ECG system GE Healthcare

15. MARS Holter analysis system GE Healthcare

16. Cardioassist, ECG system Maestros Mediline

17. CardioWin, 12 lead ECG system Genesis Medical Systems

18. SpiroWin, Spirometry system Genesis Medical Systems

19. Vivid 7 Dimension ’06 Ultrasound GE Healthcare

20. Polygraph Medicaid, Chandigarh

Recognition(s):

Recognised as Scientific and Industrial Research Organizations (SIROs), by the Department of Scientific & Industrial Research, Ministry of Science & Technology, Govt. of India

Year of Initial Approval: 2006 Recognition No.: 14/408/2005-TU-V Dated: 20th July 2006 Current Validity: From 07.07.2006 to 31.03.2009

Accredited as R & D Institution by CCRAS (Awaited)

PUBLICATIONS:

1. Acharya Balkrishna, Upadhyay A.K., Yadav A.; “Importance of Prayanama in modern life, A Scientific Review” Ayush Tomorrow,2005;1(1): 1-20

2. Acharya Balkrishna, Upadhyay A.K., Yadav A, Pant J. “An introduction of Diabetes & Physio Chemical Analysis of Active ingredients in Madhunanshini Vati” Ayush Tomorrow,2005;1(2): 25-29

3. Acharya Balkrishna, Upadhyay A.K., Ruchita T.Upadhyay “Effect of Pranayama [Voluntary Regulated Yoga Breathing] and Yogasana [Yoga Postures] in Diabetes Mellitus (DM): A Scientific Review” Journal of Complementary and Integrative Medicine, MS #1114, 2008, [The Berkeley Electronic Press, Berkeley, CA 94705]

PUBLICATIONS: (Contd.)

4. Acharya Balkrishna, Upadhyay A.K.; “Assessment of favorable and unfavorable responses from Indian System of Medicine (ISM) Professionals towards ISM as a Total Health System” Indian Journal of Psychiatry, 2008 [Under Review] [Medknow Publications, Mumbai, India]

5. Acharya Balkrishna, Upadhyay A.K., Ruchita T.Upadhyay, Kaushal K., Kumar A;” “Effect of Pranayama [Voluntary Regulated Yoga Breathing] and Yogasana [Yoga Postures] on lipid profile of normal healthy footballers” ” International Journal of Yoga, 2008 [Under Review] [Medknow Publications, Mumbai, India]

6. Acharya Balkrishna, Upadhyay A.K., “Physics & Physiology of Pranayama” in “Health for all through Ayurveda” seminar organized by Deptt. Of ISM & H, Govt. of Uttarakhand.

7. R Gokal, A Upadhyay, B Acharyaji, Ramdev Swami. The role of Yog, Pranayam and Ayurved in the prevention and treatment of Chronic Kidney Disease (CKD). 6th Annual Conference on Prevention in Renal disease, Toronto, September 28-29, 2007.

PUBLICATIONS: (Contd.)

1. Immediate effect of high-frequency yoga breathing on attentionAuthors:Shirley Telles, P. Raghuraj, Dhananjay Arankalle, K. V. NaveenPublished in: Indian Journal of Medical Sciences, 2008 [In Press][Medknow Publications, Mumbai, India]

2. An Ayurvedic basis for using honey to treat herpesAuthors:Shirley Telles, P. Raghuraj, K.V. NaveenPublished in: Medical Science Monitor, 2007; 13(11):LE17-17.[International Scientific Literature Inc., New York, USA]

3. Positive impact of yoga and pranayam on obesity, hypertension, blood sugar, and cholesterol: a pilot assessment Authors:Gokal Ram, Louise Shillito, A Upadhyay and Swami Ramdev Maharaj Published in: J Altern Complement Med. 2007;13(10):1056-8.[Mary Ann Liebert Publishers, USA]

PUBLICATIONS: (Contd.)

Hard Exudates and Macular Edema

(CSME)

Intraocular steroid injections attempt to reduce edema

4. Effects of Yoga and an Ayurveda preparation on gait, balance and mobility in older persons Authors:N.K. Manjunath, Shirley TellesPublished in: Medical Science Monitor, 2007 Dec;13(12):LE19-20.[International Scientific Literature Inc., New York, USA] 5. Effects of yoga on natural killer cell counts in early breast cancer patients undergoing conventional treatmentAuthors:Raghavendra Rao, Shirley Telles, H.R. Nagendra & othersPublished in: Medical Science Monitor, 2008; [In Press][International Scientific Literature Inc., New York, USA]

PUBLICATIONS: (Contd.)

6. In-vivo toxicological study of Drishti eye dropAuthors:Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K Published in:Progressive Agriculture,2008 [In Press]

7. Detection & Identification of alkaloid of RICINUS COMMUNIS LINN. by newly develop Thin Layer Electrochromatographic method.Authors:Kumar, A; Gaurav, S.S; Sirohi S.PS, Acharya, B.K and Sharma, S.Published in:Progressive Research,2008 [In Press]

8. In-vivo toxicological study of extract of plant Ricinus Communis Linn.for development of plant extract as safe ,accurate & effective new anticancer and anti HIV agentAuthors:Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K Published in:Progressive Agriculture,2008 [In Press]

Patents filed/ sealed:

1. Development of new extraction system for the extraction of Active Principles of Amla without denaturing the basic molecules. [“2114/DEL/2007”]2. A novel method of detection and differentiation of giloy satwa (Tinospora cordifolia) from common adulterants such as potato and maize satwa. [CBR NO. 5457]3. A novel herbal liquid solution “Drishti” for various ophthalmic disorders. Also submitting the PCT application. [ 94/DEL/2008]4. “Detection, Identification and Differentiation of urine of cow of Indian breed amongst the urines of other breed cow urine, goat and buffalo urine.” [CBR NO. 279]

Patents filed/ sealed: (Contd.) 5. A novel field test kit which enables spot test for the detection

and identification of Raw Herbs Active Constituent in the field within a few minutes. [109/DEL/2008]

6. “Isolation, Identification and characterization of specific chemical compound marker of 1000.8 KD in shilajit sample responsible for the therapeutic activity of shilajit prescribed in ayurveda as panacea”. [111/DEL/2008]

7. “A novel breathes and posture mechanism which expel the various reactive oxygen species through nasal track.” [112/DEL/2008]

8. Apparatus and method for the housing of toothbrush for prevention and destruction of microbial load deposited onto the bristle and surrounding area of used toothbrushes by novel kit for oral hygiene. [C.B.R NO.1155]

Collaboration (s):

DYMT & PYPT has collaborations with various institutions like SVYASA (Swami Vivekananda Yoga Anusandhan Samsthan), Bangalore, Patanjali Ayurved Limited, Haridwar, BEG & C, Roorkee, Divya Prem Sewa Mission, Haridwar etc for Research & Development in Yoga, Ayurveda and Ancient Indian Wisdom.

Technologies Commercialized:

Process Development of Herbo-Mineral Preparations adopting Modern Technology:Divya Pharmacy is engaged in the development and commercialization of our research. In this direction Divya Pharmacy engineered some extraction plants, vessels and many small machines which give the speed of modern manufacturing with out affecting the traditional methods of Drug preparations. The reasons for preferring Divya Pharmacy engineered machines& own process are the following; Superior Product: * Un- degraded extracts with delicacy and freshness close to nature * High concentration of desired active components * No residual solvent * Longer shelf life * Free of biological (microbial) contamination Superior Process: * Simultaneous fractionation of extract in a single step * Flexible operating conditions for multiple product extraction.

A view of Quality assurance and research lab at Divya Pharmacy

Thanks and Credits

American Optometric Association - www.aoa.org Vision Service Plan (VSP) – www.vsp.com Eye Care Community Outreach (ECCO)—

– Kelli Barker, MSW and Dewana Allen, MPH– Helping Hoosiers find and afford eye care.

Vic Malinovsky, OD, FAAO - photography – Chief of Ophthalmic Disease Services at Indiana University School of

Optometry Edwin C. Marshall, OD, MS, MPH- design

– Indiana University - Associate Dean for Academic Affairs and Student Administration, Professor of Optometry, and Adjunct Professor of Public Health, School of Medicine

Jack Downey, OD- Consultant– Indianapolis Eye Care Center

Andrew Anderson, OD- design– Gabriele Eye Institute- (574)-254-0700A view of Diagnostics and research lab at Patanjali Yog Peeth

Highly sophisticated instruments like HPTLC and ICP at Research & Development lab at D-38 Industrial area, Haridwar

Questions???

THANKS!!!