chymotrypsin proteases classnucleophile(s)base covalent intermediate serine protease cysteine...
Post on 19-Dec-2015
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Chymotrypsin
Proteases
Class Nucleophile(s) BaseCovalent Intermediate
Serine protease
Cysteine protease
Aspartyl protease
Metalloprotease (Zn)
Ser/water
Cys/water
water
water
His
His
Asp
Glu
Yes
Yes
No
No
Proton Shuttle in Carbonic Anhydrase
RNA Secondary and Tertiary Structure
Hepatitis Delta Virus Ribozyme
Hydrolysis of a phosphodiester bond by a Ribozyme
Clone and test forRibozyme that Hydrolyzes ATP
(RT-PCR)
Genetic diversity
More genetic diversity!
in vitro Evolution of a Ribozyme
(affinity chromatography)