WEDNESDAY, SEPTEMBER 6 10.5

means of logistic regression. Results: The pregnancy rate was 14.6% with a clinical pregnancy rate of 5.2% in group 1,20.9% with a clinical pregnancy rate of 18% for patients in group 2 and 29.0% with a clinical pregnancy rate of 22.1% for patients in group 3. Conclusion: Overall there was firm statistical evidence that a quarter LAH is more effective in improving the pregnancy rate, when compared with partial and total LAH.

P3.10.02 A PROSPECTIVE RANDOMIZED CONTROLLED STUDY ON THE COMPARISON OF THE RESULTS BETWEEN THE DAY-3 EMBRYO TRANSFER AND DAY-S BLASTOCYST TRANSFER T. Utsunomiva, C. Ikeda, K. Hirai, K. Hirotsuru, Dept. OBIGYN, St. Luke Clinic, Oita City, Japan.

Objectives: To determine the effectiveness of day-3 and day-5 ET on the pregnancy and cancellation rate following in vitro fertilization (IVF) and intracytoplasmic sperm injection. Study Methods: One hundred sixty four cycles in patients (average age: 34 (25-45)) were treated by IVF and ICSI. All zygotes were cultured to day-3 in HTF for day-5 in Blastocyst medium or Gl/G2 medium. Pregnancy rate and cancellation rate were compared between the groups of day-3 ET and day-5 ET. Results: The results were as follows: the pregnancy rate (cycles) of day- 3 ET was 18.3% and day-5 ET was 14.8%. The cancellation rate of day- 3 ET was 2.4% and day-5 ET was 25.6%. Conclusions: The pregnancy rate of conventional day-3 ET using HTF medium was better than the new method as day-5 ET using blastocyst stage culture medium. The theories of blastocyst stage culture and day-5 ET might be very good and ideal procedures. From our results, however, the condition of blastocyst stage culture should be discussed more.

P3.10.03 CAUSES OF INFERTILITY IN INFERTILITY CENTER OF MASHHAD UNIVERSITY, IRAN FROM 1998 TO 2000 D.M. Dehnhani, D.Z. Yousefi, D.H. Salare, D.M. Amere, Dept. OBIGYN, Ghaem Hospital, Mashhad University, Khorasan, Iran.

Objectives: Evaluation of Causes of infertility in infertility center Mashhad University, Iran. Study Method: 1846 patients with infertility were evaluated with retrospective method. 1515 patients had primary infertility and 331 patients had secondary infertility. Result: 654 patients were with male factors and 715 patients had female factors and 248 patients with male and female factors and 238 patients with unexplained factors. Conclusion: Ovulatory disorder was the main cause for female factors, whereas varicocle the main cause for male factors.

P3.10.04 CHROMOSOMAL ANALYSIS OF FAILED-FERTILIZED HUMAN OOCYTES RESULTING FROM IN-VITRO FERTILIZATION AND INTRACYTOPLASMIC SPERM INJECTION S. 0. Makemaharn, S. Suksompong, Dept. OBIGYN, Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Objectives: The aim of the study was to evaluate the incidence of chromosomal abnormalities in “failed -fertilized” oocytes derived from in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure. Study Methods: 164 oocytes from 62 IVF patients and 64 oocytes from 32 ICSI patients were selected on the basis of availability of oocytes and staff. Chromosomal analysis was performed using the method described by Tarkowski. Results: 111 (67.7%) of the IVF and 56 (87.5%) of the ICSI oocytes were successfully karyotyped. Of 111 IVF oocytes, 73 (65.8%) exhibited normal haploid and 38 (34.2%) were abnormal. The abnormalities included 25 aneuploid (22.5%), 11 diploid (9.9%) and 2 structural anomalies (1.8%). Of 56 ICSI oocytes, 52 (92.9%) were normal haploid and only 4 (7.17) D were aneuploid. The sperm nuclei were observed in 43 IVF oocytes (38.7%), composed of 38 (34.2%) premature chromosome condensation (PCC) and 5 (4.5%) decondensed

sperm heads. Evidence of successful sperm delivery was found in all 56 ICSI oocytes; 25.0% (14156) showed PCC, 17.9% (10156) showed decondensed sperm heads, and 57.1% (32156) showed intact sperm heads. Conclusions: This study suggested that about one-third of unfertilized oocytes exhibited chromosomal abnormalities. The difference of aneuploidy between IVF and ICSI oocytes needs further studies analyzing larger number of oocytes.

P3.10.05 COMPARISON OF OVULATION INDUCTION WITH TWO DIFFERENT RECOMBINANT FOLLICLE-STIMULATING HORMONE PRODUCTS (PUREGON 50 AND GONAL F 75) FOR IN-VITRO FERTILIZATION N. Kanakas (l), T. Mantzavinos (2), K Arvaniti (l), G. Creatsas (2) Euromedica IVF Unit, Athens, Greece. 2”d Dept. OBIGYN, University of Athens, Athens, Greece.

Objective: Using the recombinant technology a new product of FSH has been widely introduced in the IVF programs. The purpose of this study was to compare the two commercially available recombinant FSH hormones regarding the IVF outcome and pregnancy rate. Study Methods: Retrospective data analysis between the two ret FSH hormones in an IVF centre. This study was carried out in 107 cycles (group A) receiving Puregon 50 IU (Organon, The Netherlands) and in 102 cycles receiving Gonal F 75 IU (Serono, Switzerland) (group B) who participated in a comparative study between June 1997 and December 1999. Ovulation was induced using the same short protocol of GnRH analogue (Buserelin, 1.2 mgriday) and ret FSH was administered starting on day three of the cycle. Both products were administered intramuscularly. A similar number of patients had their oocytes feritilized with the classical IVF method and the intracytoplasmic method (ICSI) in both groups. The mean age of the patient was 34.77 years and 35.06 for group A and B respectively. Results: There were no statistical significant differences observed in the average number of ampoules administered per cycle (27.37 f 8.43 Puregon 50 IU and 28.69 f 9.7 Gonal F 75 IU), in the duration of treatment 7.97 f 1.59 days (Puregon 50) and 8.71 f 1.80 days (Gonal F 75), in the cancellation rate, in the oocytes recovery rate (6.06 f 4.3 Puregon 50 - 5.82 f 4.3 Gonal F 75) and in the number of embryos transferred between group A and B (2.7 f 2.02 and 2.59 f 1.73). No severe hyperstimulation was observed in both groups. Estradiol on the day of HCG administration did not differ between both groups (1435.21 f 931.6 pg/ml in Puregon 50 IU group compared to 1608 f 720.3 pgiml, in Gonal F 75 group). Clinical pregnancy rates per embryo transfer did not differ between both groups (19.1% for group A and 20% for group B). Conclusions: Administration of the two different ret FSH products gave similar results in a significant number of cycles.

P3.10.06 DIMINISHED T CELL ADHESION TO EXTRACELLULAR MATRIX PROTEINS IN WOMEN WITH ANTIPHOSPHOLIPID ANTIBODIES M.Jerzak, W.Baranowski, J.Pajak, A.Gorski, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Weigla Street 12, Wroclaw, Poland, 53-114.

Objectives: Recent data emphasize the important role of extracellular matrix (ECM) proteins in the regulation of T cell function. Evidence is accumulating that activation of the immune system can influence implantation and may be responsible for unexplained infertility and pregnancy loss. Our previous data suggest the association of abnormally high T cell adhesion to ECM proteins with recurrent spontaneous abortion (RSA). The aim of this study was to determine T cell adhesion to ECM proteins in antiphospholipid (APA) positive women. Study Methods: Thirty women with unexplained infertility, including women with RSA, and ten normal healthy women with the previous successful pregnancy outcome were studied. We investigated phorbol acetate myristate (PMA) and phytohemaglutinin (PHA) activated peripheral blood T cell adhesion to the following proteins of extracellular matrix: collagen IV (C-IV), elastin (E) and fibronectin (FN). APA concentration was measured by ELISA method. Results: We determined that Pm-activated T cell adhesion to C-IV and