Christoph U. Germeier, Zofia Bulinska-Radomska, Lena Dimberg, Matthias H. Herrmann, Jean Koenig, Gerard Branlard, Danela Murariu, Ivana Polisenska,

Rita Redaelli, Valeria Terzi

Avena Genetic Resources for Quality in Human Consumption

(AVEQ)

Project Partners

Owies, Kraków 2009

AVEQ - Outline

Objectives

Work plan

Fusarium pathogens and mycotoxins in oats

Introduction

A European Project on Nutritional Quality in Avena Genetic Resources

Owies, Kraków 2009

AVEQ - Introduction

Targeted action in accordance with the Council regulation (EC) No 870/2004 establishing a Community programme on the conservation, characterisation, collection and utilisation of genetic resources in agriculture

Starting date: 01. 03. 2007

End date: 28. 02. 2011

Duration: 48 months

Owies, Kraków 2009

AVEQ - Objectives

Evaluation, characterisation and documentation of selected oat genetic resources with regard to:

Contents of protein, fat, minerals, starch, ß- glucan and antioxidants

Resistance to Fusarium infection and mycotoxin contamination

Cold tolerance

Grain weight, hull content and test weight

Owies, Kraków 2009

AVEQ:15 Partners from 9 countries

Co-ordinatorP0: Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Quedlinburg, GermanyProject leader – Dr. Christoph U. Germeier

P1: Plant Breeding and Acclimatization Institute (IHAR), National Plant Genetic Resources Centre Radzików, Poland

P2: Svalöf Weibull AB Cereal Breeding Department, Svalöf, Sweden

P3: Julius Kuehn Institute, Federal Research Centre for Cultivated Plants - Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany

P4: Eurofins Analytik GmbH, Hamburg, Germany

Owies, Kraków 2009

AVEQ - Partners

P 9: Vegetal Genetic Resources Bank of Suceava, Suceava, Romania

P8: CRA-GPG, ex Istituto Sperimentale per la Cerealicoltura, Fiorenzuola d'Arda, Italy

P5: Institut National de la Recherche Agronomique (INRA), UMR Amélioration

et Santé des Plantes, Clermont-Ferrand, France

P6: CRA-MAC, ex Istituto Sperimentale Cerealicoltura, Bergamo, Italy

P7: Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden

Owies, Kraków 2009

AVEQ - Partners

P11: Jõgeva Plant Breeding Institute, Jögeva, Estonia

P10: Institute of Plant Genetic Resources “K. Malkov”, Sadovo, Bulgaria

P12: Plant Breeding and Acclimatization Institute - Laboratory of Nutritional Evaluation of Plant Materials, Radzików , Poland

P14: Agricultural Research Institute Kromeriz, Ltd., Czech Republic

P15: Agrotest Fyto, Ltd., Kromeriz, Czech Republic

Owies, Kraków 2009

AVEQ - Materials

Working collection of 600 accessions of Avena genetic resources of different improvement status :

accessions of wild species

selected land races

modern cultivars

Owies, Kraków 2009

Methods – Workpackages (WP)

WP 1: Management and Coordination- Julius Kuehn Institute, Institute for Breeding

Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0)

WP 2: Project working collection - IHAR, Poland, Zofia Bulinska-Radomska (P1);

P0, P5, P6, P8, P9, P10

Plan: 80% cultivated material - 200 landraces, 200 obsolete cultivars, 100 modern cultivars

20% wild Avena species (100 accessions).

WP 2: Project working collection

Species 2008 2009

A. sativa 287 256

A. byzantina 8 16

A. strigosa 15 31

A. abyssinica 2 3

A. barbata 3 1

A. canariensis - 1

A. damascena 2 -

A. fatua 7 9

A. hirtula 1 3

A. hybrida 1 -

A. sterilis 2 3

A. wiestii 1 -

329 323

Wild species not obtained

A. agadiriana 7

A. atlantica 10

A. insularis 1

A. longiglumis 16

A. macrostachya 2

A. murphyi 16

A. occidentalis 13

A. pilosa 9

A. prostrata 13

A. vaviloviana 12

A. ventricosa 8

11

WP 2: Origin of the Project Working Collection

Legend

1 - 2

3 - 4

5 - 7

8 - 10

11 - 14

15 - 20

21 - 24

25 - 34

35 - 38

39 - 66

Owies, Kraków 2009

Methods - Workpackages

WP 3: Field experiments and sampling seeds for quality analysis

- Vegetal Genetic Resources Bank of Suceava, Romania, Danela Murariu (P9); P1, P2, P5, P8, P10, P11

Field experiments laid out as augmented block designs Plot size from 2.0m2 to 3.0m2

establishing plots, taking observations in the field and on harvested material - descriptors

13

IHAR

SVGB

IPGR

INRA

SW

CRA-ISC

PBI

Descriptors observed in field experiments

- Days to heading- Days to maturity- Plant height- Lodging- Panicle shape- Occurrence of

diseases- Colour of lemma- Hull content- Yield- Thousand kernel

weight- Test weight

Obligatory: Optional:

- Emergence- Number of fertile tillers - Number of seeds per

panicle

Owies, Kraków 2009

Owies, Kraków 2009

Methods – Workpackages

WP 4: Field experiments with artificial Fusarium inoculation

- Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany, Matthias H. Herrmann (P3); P8 (Italy), P9 (Romania), P14 (Czech Republic)

Field trials, inoculation with Fusarium at 3 locations, in Italy

natural infection Observations in the field and on harvested material Samples for mycotoxin analysis

Owies, Kraków 2009

Methods – Workpackages

WP 5: Fusarium and mycotoxin analysis - Agrotest Fyto, Ltd., Kromeriz, Czech Republic,

Ivana Polisenska (P15); P4, P6, P8

analysis of mycotoxin content (DON, T-2, HT-2): ELISA,

LC-MS/MS

determination of Fusarium species: PCR

Owies, Kraków 2009

Fusarium on oats – basic facts

the symptoms of the infection on panicles in the field are only rarely observable (Gilbert and Tekauz, 2000)

newly described species F. langsethiae (Torp and Adler, 2004; Torp and Nirenberg, 2004) is quite often present on oats - no symptoms, T-2 and HT-2 production

Parrika et al. (2006) - in Finland: F. langsethiae, F. poae, F. culmorum,

F. graminearum, F. avenaceum Imathiu et al. (2006) - in GB: mainly F. langsethiae on oats Kiecana and Perkowski; Kiecana and Mielniczuk (2001) – in Poland:

F. avenaceum, F. culmorum, F. poae, F. sporotrichioides and

F. crookwellense

Owies, Kraków 2009

AVEQ = Avena genetic resources for quality in human consumption

Why DON, T-2 and HT-2 in AVEQ?

DON ZEA (ppb) (ppb)

unprocessed cereals other than durum wheat, oats and maize 1250 100

unprocessed durum wheat and oats 1750 100

DON and ZEA have the same producer – F.graminearum and F.culmorum

Owies, Kraków 2009

Why DON, T-2 and HT-2 in AVEQ?

T-2 and HT-2 are mentioned, but without limits - till now

(31) …… the presence of T-2 and HT-2 toxin can be of concern for public health. Therefore, the development of a reliable and sensitive method, collection of more occurrence data and more investigations/research in the factors involved in the presence of T-2 and HT-2 toxin in cereals and cereal products, in particular in oats and oat products, is necessary and of high priority.

Owies, Kraków 2009

Fusarium and mycotoxins in AVEQ

Field trials, inoculation with F.culmorum, F.graminearum, F.sporotrichioides, F.langesethiae and F.avenaceum on 3 locations, in Italy natural infection

Observations in the field and on harvested material

Mycotoxin analysis – analytical problems: (ELISA vs. LC-MS/MS) – comparison study

Harmonisation of laboratories

inoculated non -inoculated

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Owies, Kraków 2009

ELISA vs. LC-MS/MS, DON

AVEQ standard cultivars

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Owies, Kraków 2009

ELISA vs. LC-MS/MS, T-2

inoculated non -inoculated

AVEQ standard cultivars

Owies, Kraków 2009

Fusarium spp. on oat

cv. Belinda

Owies, Kraków 2009

Analysis for protein (including Avenin patterns), fat and minerals

Analytical WP: 6 - 8

Analysis for starch, fibre and ß- Glucan

Analysis for avenanthramides and tocols

WP 9: Analysis for cold and drought tolerance- Italy, Romania

Owies, Kraków 2009

WP 10: Project documentation and Internet portal

- Julius Kuehn Institute, Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany, Christoph U. Germeier (P0)

http://eadb.bafz.de/aveq/

All results will be made available online in a crop portal for Avena based on the European Avena Database (EADB).

Owies, Kraków 2009

Thank you for your attention

Acknowledgements

Peter Koelln KGaA, Elmshorn, Germany

Emco Ltd., Praha, Czech Republic

Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP), Bonn, Germany

European Commission, DG AGRI council regulation 870/2004 .